Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011815

Physical Location & Seq

Genomic Coordinates Forward (+)

19937300 .. 19944294

Gene Structure 6995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1242400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGATCTCGGACGGAGTTGAAGATGAAGAGAAATGGCTGGCAGCTGGAATCGCCGGCCTCCAGCAAAACGCCTTCTACATGCATCGTGCTCTGGACTCCAACAATCTCAGAGATGCCCTCAAGTATTCTGCTCAGATGCTCTCGGAGCTCCGGACCTCCAAGCTTTCCCCGCACAAGTACTACGAACTCTATATGCGGGCGTTTGACGAATTGAGGAAATTGGAAATGTTCTTCAAGGAGGAGGCTCGGCGCGGTTGCTCCATTATCGATCTCTATGAGCTCGTCCAGCACGCAGGCAACATATTGCCTAGATTGTATCTTCTCTGTACAGTAGGATCTGTGTATATCAAATCTAAGGAAGCTCCTGCTAAGGATGTCCTGAAAGATCTTGTGGAAATGTCCCGTGGCATTCAGAATCCTGTACGTGGGCTATTCTTAAGGAGTTACCTTTCTCAAGTGAGTAGGGATAAATTGCCTGATATTGGTTCTGAATATGAAGGAGATGCTGACACTGTCAAGGATGCTGTTGAGTTTGTTCTCCAAAATTTCACTGAGATGAACAAACTTTGGGTGCGGATGCAACATCAGGGACCTGCCCGGGAAAAGGAGAAGCGGGAGAAAGAAAGGAGCGAGCTACGTGATCTTGTTGGTAAAAACTTGCATGTCCTCAGCCAGATAGAAGGTGTTGATCTGGATTTGTACAAAGATACGGTGCTTCCCAGAGTCTTGGAACAGGTTGTCAATTGTAAGGATGAGCTTGCACAGTTTTATTTGATGGATTGCATAATTCAAGTCTTTCCTGATGAGTACCACTTGCAAACTCTTGATGTATTGTTAGGTGCCTGCCCCCAACTTCAGCCATCTGTTGATATCAAGACAGTTCTATCCCAGTTGATGGAAAGACTTTCAAATTATGCTGCTTCAAGTACAGAAGTGCTGCCTGAGTTTTTACAAGTAGAAGCTTTTTCAAAACTGAGCAATGCTATTGGAAAGGTGATTGAAGCACAAGTTGACATGCCTATCATCGGTGTTGTAACTCTATATTCATCTCTTCTTAAATTTACCCTCCATGTGCACCCTGATCGACTTGATTATGCTGACCAAGTGTTGGGCTCATTTGTTAAGAAACTCTCTGGAAAAGGGAAAATTCAAGACAGCAGGGCAACAAAACAAGTGGTTGCACTTTTAAGTGCTCCACTTGAGAAATATAATGATATTGTCACGGCCTTGAAGCTTACAAATTATCCTCGTGTCCTGGAATTTCTTGATAGTGGAACAAATAAAGTCATGGCTACTGTTATAATTCAGAGCATTATGAAAAATACAACTCATGTTTTAACTGCTGAGAAGGTTGAGGCATTATTTGAATTAATAAAAGGACTTATTGAGGATTTGGATGGGCCTCTTAATGATGAGGTTGATGAAGAGGATTTCAAGGAGGAGCAAAATTCAGTTGCGCGACTTATTCAAATGTTTTCTAATGATGATTCCGAAGAGATGTTTAAGATCATATGTACTGTGAAGAAGCACATCCTGAATGGAGGACCAAAGCGTTTGCCTTTTACTGTCCCTCCCCTAGTTTTCTCTTCTCTAAAGTTGGTTAGGAAATTGCAAGCCCAGGAAGAGAATCCATTTGGAGATGATGCATCAACCACACCAAAGAAAAATTTTCAGCTCTTAACTCAGACTATTGAGGCTCTGTTGAATGTTCCAGCACCTGAACTGGCATTACGGTTATACTTGCAATGTGCAGAGGCTGCAAATGACTGTGATTTGGAACCTGTTGCATATGAGTTCTTCACCCAAGCATATATTCTTTACGAAGAAGAAATTTCAGATTCGAAAGCACAGGTGACTGCCATTCATCTGATAGTTGGGACTCTCCAGAGGATGCATGTATTTGGTGTTGAAAACAGGGATACTTTAACTCACAAAGCCACAGGGTATTCGGCAAAGCTTTTAAAGAAGGCTGATCAGTGCCGAGCTGTTTATGCTTGTGCACATCTCTTCTGGGTTGACGATCAGGAGAACATGAAGGATGGAGAAAGGGTACTGATTTGCCTAAAGCGAGCTTTAAGAATTGCCAACGCTGCCCAGCAGATGTCCAATGCTACACGGGGTAGCACCGGTTCTGTGGCGCTCTTTGTTGAAATACTGAACAAGTACCTTTATTTCTTTGAGAAGGGGAACCCACAGATAACGGTTGCTTCAGTTCAAAGCCTGATTGAATTGGTTACAACTGAACTGCAAAGCGACTCGGCCTCAGCAGAGCCATCTGTTGATGCTTTCTTTGCCAGCACACAGCGGTACATCCAGTTCCAGAAGCAGAAAGGCGGCGCAGTTGGTGAAAGATATGAGCCCATCAAAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0010008 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0016482 GO:0030904 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042147 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Vps35

Protein Analysis

791

Amino Acids

89.16

Weight (kDa)

5.34

Isoelectric Point (pI)

42.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps35	PF03635	12 - 747	2.1e-288	Vacuolar protein sorting-associated protein 35

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17790 AT3G51310
fragaria_vesca	FvH4_1g11410
malus_domestica	MD02G1126400.v1.1 MD15G1242400.v1.1
prunus_persica	Prupe.7G171800_v2.0.a1 Prupe.7G171800_v2.0.a1
pyrus_communis	pycom02g09870 pycom15g21360
rosa_chinensis	RchiOBHm_Chr2g0098981
rosa_laevigata	RLG00000016838
rosa_multiflora	Rmu_sc0000577.1_g000009
rosa_roxburghii	Rroxscaffold_2G00143530
rosa_rugosa	Rorug02G0075400
rosa_samantha	Rh2AG124900 Rh2BG128100 Rh2CG129000 Rh2DG129200
rosa_wichuraiana	Rw2G009590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1301
Acc36I	ACCTGC	1 cut(s)	601
AccB1I	GGYRCC	1 cut(s)	839
AccB7I	CCANNNNNTGG	2 cut(s)	895, 1108
AccII	CGCG	2 cut(s)	252, 1459
AccIII	TCCGGA	1 cut(s)	150
AciI	CCGC	7 cut(s)	170, 196, 252, 574, 613, 2305, 2334
AclWI	GGATC	1 cut(s)	343
AcsI	RAATTY	7 cut(s)	544, 1058, 1146, 1259, 1447, 1666, 1830
AcuI	CTGAAG	2 cut(s)	839, 2193
AfiI	CCNNNNNNNGG	6 cut(s)	425, 482, 604, 895, 1025, 1108
AflII	CTTAAG	1 cut(s)	436
AgeI	ACCGGT	1 cut(s)	2126
AjnI	CCWGG	2 cut(s)	1254, 1617
AjuI	GAANNNNNNNTTGG	2 cut(s)	1797, 1829
Alw21I	GWGCWC	6 cut(s)	91, 150, 282, 1077, 1195, 2002
Alw44I	GTGCAC	2 cut(s)	1073, 1998
AlwI	GGATC	1 cut(s)	343
AlwNI	CAGNNNCTG	2 cut(s)	1718, 1757
Ama87I	CYCGRG	1 cut(s)	597
Aor13HI	TCCGGA	1 cut(s)	150
AoxI	GGCC	4 cut(s)	55, 1224, 1400, 2259
ApaLI	GTGCAC	2 cut(s)	1073, 1998
ApeKI	GCWGC	5 cut(s)	41, 917, 937, 1757, 2090
ApoI	RAATTY	7 cut(s)	544, 1058, 1146, 1259, 1447, 1666, 1830
AseI	ATTAAT	1 cut(s)	1370
AsiGI	ACCGGT	1 cut(s)	2126
AspLEI	GCGC	4 cut(s)	252, 1459, 2140, 2339
AspS9I	GGNCC	4 cut(s)	153, 590, 1400, 1544
AsuC2I	CCSGG	2 cut(s)	598, 599
AsuHPI	GGTGA	4 cut(s)	1006, 1792, 1864, 2357
AsuII	TTCGAA	1 cut(s)	1841
AvaI	CYCGRG	1 cut(s)	597
AvaII	GGWCC	3 cut(s)	153, 590, 1544
BaeGI	GKGCMC	2 cut(s)	1077, 2002
BanI	GGYRCC	1 cut(s)	839
BanII	GRGCYC	4 cut(s)	150, 282, 1115, 2361
BauI	CACGAG	1 cut(s)	1248
Bbv12I	GWGCWC	6 cut(s)	91, 150, 282, 1077, 1195, 2002
BbvCI	CCTCAGC	2 cut(s)	668, 2263
BbvI	GCAGC	5 cut(s)	53, 904, 924, 1744, 2077
BccI	CCATC	7 cut(s)	769, 868, 889, 1391, 2033, 2281, 2369
BceAI	ACGGC	1 cut(s)	1239
BcgI	CGANNNNNNTGC	2 cut(s)	1064, 1098
BciT130I	CCWGG	2 cut(s)	1256, 1619
BciVI	GTATCC	1 cut(s)	1912
BclI	TGATCA	1 cut(s)	1972
BcnI	CCSGG	2 cut(s)	598, 599
BfaI	CTAG	2 cut(s)	309, 1577
BfoI	RGCGCY	1 cut(s)	2141
BfrI	CTTAAG	1 cut(s)	436
BfuAI	ACCTGC	1 cut(s)	601
BfuI	GTATCC	1 cut(s)	1912
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	6 cut(s)	42, 918, 938, 1758, 2091, 2335
BlsI	GCNGC	6 cut(s)	43, 919, 939, 1759, 2092, 2336
BmcAI	AGTACT	1 cut(s)	179
Bme1390I	CCNGG	4 cut(s)	598, 599, 1256, 1619
Bme18I	GGWCC	3 cut(s)	153, 590, 1544
BmeT110I	CYCGRG	1 cut(s)	597
BmgT120I	GGNCC	4 cut(s)	153, 590, 1400, 1544
BmiI	GGNNCC	4 cut(s)	591, 841, 1779, 2189
BmrFI	CCNGG	4 cut(s)	598, 599, 1256, 1619
BmrI	ACTGGG	1 cut(s)	883
BmuI	ACTGGG	1 cut(s)	883
BplI	GAGNNNNNCTC	2 cut(s)	102, 134
BpmI	CTGGAG	2 cut(s)	44, 1868
Bpu10I	CCTNAGC	3 cut(s)	369, 668, 2263
Bpu14I	TTCGAA	1 cut(s)	1841
BpuEI	CTTGAG	3 cut(s)	104, 438, 1220
BpuMI	CCSGG	2 cut(s)	598, 599
Bsa29I	ATCGAT	1 cut(s)	267
BsaAI	YACGTR	2 cut(s)	425, 638
BsaJI	CCNNGG	3 cut(s)	403, 597, 1617
BsaWI	WCCGGW	2 cut(s)	150, 2126
BsaXI	ACNNNNNCTCC	4 cut(s)	1555, 1585, 2034, 2064
Bsc4I	CCNNNNNNNGG	6 cut(s)	425, 482, 604, 895, 1025, 1108
Bse118I	RCCGGY	2 cut(s)	53, 2126
Bse1I	ACTGG	3 cut(s)	889, 1728, 2314
Bse3DI	GCAATG	2 cut(s)	985, 1751
BseAI	TCCGGA	1 cut(s)	150
BseBI	CCWGG	2 cut(s)	1256, 1619
BseCI	ATCGAT	1 cut(s)	267
BseDI	CCNNGG	3 cut(s)	403, 597, 1617
BseGI	GGATG	9 cut(s)	379, 526, 582, 757, 1402, 1530, 1896, 2044, 2310
BseLI	CCNNNNNNNGG	6 cut(s)	425, 482, 604, 895, 1025, 1108
BseMI	GCAATG	2 cut(s)	985, 1751
BseMII	CTCAG	9 cut(s)	121, 146, 543, 682, 933, 965, 1335, 1697, 2277
BseNI	ACTGG	3 cut(s)	889, 1728, 2314
BseRI	GAGGAG	2 cut(s)	254, 1454
BseSI	GKGCMC	2 cut(s)	1077, 2002
BseXI	GCAGC	5 cut(s)	53, 904, 924, 1744, 2077
BseYI	CCCAGC	1 cut(s)	2094
BsgI	GTGCAG	1 cut(s)	1770
Bsh1236I	CGCG	2 cut(s)	252, 1459
BshFI	GGCC	4 cut(s)	57, 1226, 1402, 2261
BshNI	GGYRCC	1 cut(s)	839
BshTI	ACCGGT	1 cut(s)	2126
BshVI	ATCGAT	1 cut(s)	267
BsiHKAI	GWGCWC	6 cut(s)	91, 150, 282, 1077, 1195, 2002
BsiHKCI	CYCGRG	1 cut(s)	597
BsiSI	CCGG	4 cut(s)	54, 151, 598, 2127
BslFI	GGGAC	4 cut(s)	385, 603, 1553, 1891
BslI	CCNNNNNNNGG	6 cut(s)	425, 482, 604, 895, 1025, 1108
BsmFI	GGGAC	4 cut(s)	385, 603, 1553, 1891
BsmI	GAATGC	1 cut(s)	408
BsnI	GGCC	4 cut(s)	57, 1226, 1402, 2261
BsoBI	CYCGRG	1 cut(s)	597
Bsp119I	TTCGAA	1 cut(s)	1841
Bsp1286I	GDGCHC	8 cut(s)	91, 150, 282, 1077, 1115, 1195, 2002, 2361
Bsp13I	TCCGGA	1 cut(s)	150
Bsp1407I	TGTACA	2 cut(s)	326, 699
BspACI	CCGC	7 cut(s)	170, 196, 252, 574, 613, 2305, 2334
BspANI	GGCC	4 cut(s)	57, 1226, 1402, 2261
BspCNI	CTCAG	9 cut(s)	120, 145, 544, 681, 934, 966, 1336, 1696, 2276
BspDI	ATCGAT	1 cut(s)	267
BspEI	TCCGGA	1 cut(s)	150
BspFNI	CGCG	2 cut(s)	252, 1459
BspLI	GGNNCC	4 cut(s)	591, 841, 1779, 2189
BspMI	ACCTGC	1 cut(s)	601
BspPI	GGATC	1 cut(s)	343
BspT104I	TTCGAA	1 cut(s)	1841
BspT107I	GGYRCC	1 cut(s)	839
BspTI	CTTAAG	1 cut(s)	436
BsrDI	GCAATG	2 cut(s)	985, 1751
BsrFI	RCCGGY	2 cut(s)	53, 2126
BsrGI	TGTACA	2 cut(s)	326, 699
BsrI	ACTGG	3 cut(s)	889, 1728, 2314
BssAI	RCCGGY	2 cut(s)	53, 2126
BssECI	CCNNGG	3 cut(s)	403, 597, 1617
BssSI	CACGAG	1 cut(s)	1248
Bst2BI	CACGAG	1 cut(s)	1248
Bst2UI	CCWGG	2 cut(s)	1256, 1619
Bst6I	CTCTTC	7 cut(s)	21, 1056, 1419, 1488, 1591, 1617, 2012
BstAFI	CTTAAG	1 cut(s)	436
BstAPI	GCANNNNNTGC	1 cut(s)	1757
BstAUI	TGTACA	2 cut(s)	326, 699
BstBAI	YACGTR	2 cut(s)	425, 638
BstBI	TTCGAA	1 cut(s)	1841
BstDSI	CCRYGG	1 cut(s)	403
BstF5I	GGATG	9 cut(s)	379, 526, 582, 757, 1402, 1530, 1896, 2044, 2310
BstFNI	CGCG	2 cut(s)	252, 1459
BstH2I	RGCGCY	1 cut(s)	2141
BstHHI	GCGC	4 cut(s)	252, 1459, 2140, 2339
BstMWI	GCNNNNNNNGC	8 cut(s)	50, 145, 169, 286, 1757, 1991, 2090, 2291
BstNI	CCWGG	2 cut(s)	1256, 1619
BstNSI	RCATGY	4 cut(s)	82, 665, 1018, 1898
BstSCI	CCNGG	4 cut(s)	596, 597, 1254, 1617
BstSLI	GKGCMC	2 cut(s)	1077, 2002
BstUI	CGCG	2 cut(s)	252, 1459
BstV1I	GCAGC	5 cut(s)	53, 904, 924, 1744, 2077
BstX2I	RGATCY	2 cut(s)	335, 385
BstXI	CCANNNNNNTGG	1 cut(s)	727
BstYI	RGATCY	2 cut(s)	335, 385
Bsu15I	ATCGAT	1 cut(s)	267
BsuI	GTATCC	1 cut(s)	1912
BsuRI	GGCC	4 cut(s)	57, 1226, 1402, 2261
BsuTUI	ATCGAT	1 cut(s)	267
BtgI	CCRYGG	1 cut(s)	403
BtsCI	GGATG	9 cut(s)	379, 526, 582, 757, 1402, 1530, 1896, 2044, 2310
BtsIMutI	CAGTG	3 cut(s)	510, 549, 1982
BveI	ACCTGC	1 cut(s)	601
CaiI	CAGNNNCTG	2 cut(s)	1718, 1757
CfoI	GCGC	4 cut(s)	252, 1459, 2140, 2339
Cfr10I	RCCGGY	2 cut(s)	53, 2126
Cfr13I	GGNCC	4 cut(s)	153, 590, 1400, 1544
Cfr9I	CCCGGG	1 cut(s)	597
ClaI	ATCGAT	1 cut(s)	267
CspAI	ACCGGT	1 cut(s)	2126
CspCI	CAANNNNNGTGG	2 cut(s)	1155, 1190
CviAII	CATG	9 cut(s)	79, 662, 1015, 1070, 1288, 1331, 1895, 2032, 2370
DraI	TTTAAA	1 cut(s)	1962
Eam1104I	CTCTTC	7 cut(s)	21, 1056, 1419, 1488, 1591, 1617, 2012
EarI	CTCTTC	7 cut(s)	21, 1056, 1419, 1488, 1591, 1617, 2012
Ecl136II	GAGCTC	2 cut(s)	148, 280
Eco24I	GRGCYC	4 cut(s)	150, 282, 1115, 2361
Eco32I	GATATC	1 cut(s)	871
Eco47I	GGWCC	3 cut(s)	153, 590, 1544
Eco53kI	GAGCTC	2 cut(s)	148, 280
Eco57I	CTGAAG	2 cut(s)	839, 2193
Eco88I	CYCGRG	1 cut(s)	597
EcoICRI	GAGCTC	2 cut(s)	148, 280
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRII	CCWGG	2 cut(s)	1254, 1617
EcoRV	GATATC	1 cut(s)	871
EcoT22I	ATGCAT	3 cut(s)	84, 1648, 1896
EcoT38I	GRGCYC	4 cut(s)	150, 282, 1115, 2361
FaeI	CATG	9 cut(s)	82, 665, 1018, 1073, 1291, 1334, 1898, 2035, 2373
FaqI	GGGAC	4 cut(s)	385, 603, 1553, 1891
FatI	CATG	9 cut(s)	78, 661, 1014, 1069, 1287, 1330, 1894, 2031, 2369
FauI	CCCGC	3 cut(s)	177, 189, 606
FauNDI	CATATG	2 cut(s)	1511, 1789
FbaI	TGATCA	1 cut(s)	1972
Fnu4HI	GCNGC	6 cut(s)	42, 918, 938, 1758, 2091, 2335
FokI	GGATG	9 cut(s)	386, 533, 589, 764, 1409, 1517, 1903, 2051, 2297
FriOI	GRGCYC	4 cut(s)	150, 282, 1115, 2361
Fsp4HI	GCNGC	6 cut(s)	42, 918, 938, 1758, 2091, 2335
FspBI	CTAG	2 cut(s)	309, 1577
GlaI	GCGC	4 cut(s)	251, 1458, 2139, 2338
GluI	GCNGC	6 cut(s)	42, 918, 938, 1758, 2091, 2335
GsaI	CCCAGC	1 cut(s)	2098
GsuI	CTGGAG	2 cut(s)	44, 1868
HaeII	RGCGCY	1 cut(s)	2141
HaeIII	GGCC	4 cut(s)	57, 1226, 1402, 2261
HapII	CCGG	4 cut(s)	54, 151, 598, 2127
HhaI	GCGC	4 cut(s)	252, 1459, 2140, 2339
Hin1II	CATG	9 cut(s)	82, 665, 1018, 1073, 1291, 1334, 1898, 2035, 2373
Hin6I	GCGC	4 cut(s)	250, 1457, 2138, 2337
HinP1I	GCGC	4 cut(s)	250, 1457, 2138, 2337
HincII	GTYRAC	2 cut(s)	1012, 2017
HindII	GTYRAC	2 cut(s)	1012, 2017
HindIII	AAGCTT	4 cut(s)	161, 960, 1232, 1955
HinfI	GANTC	9 cut(s)	48, 95, 415, 723, 1487, 1627, 1838, 1879, 2255
HpaII	CCGG	4 cut(s)	54, 151, 598, 2127
HphI	GGTGA	4 cut(s)	1006, 1792, 1864, 2357
Hpy166II	GTNNAC	4 cut(s)	1012, 1075, 2000, 2017
Hpy8I	GTNNAC	4 cut(s)	1012, 1075, 2000, 2017
HpyAV	CCTTC	7 cut(s)	82, 491, 674, 1342, 1960, 2029, 2176
HpyCH4IV	ACGT	2 cut(s)	424, 637
HpyF10VI	GCNNNNNNNGC	8 cut(s)	50, 145, 169, 286, 1757, 1991, 2090, 2291
HpySE526I	ACGT	2 cut(s)	424, 637
Hsp92II	CATG	9 cut(s)	82, 665, 1018, 1073, 1291, 1334, 1898, 2035, 2373
HspAI	GCGC	4 cut(s)	250, 1457, 2138, 2337
Kpn2I	TCCGGA	1 cut(s)	150
KroI	GCCGGC	1 cut(s)	53
KroNI	GCCGGC	1 cut(s)	55
Ksp22I	TGATCA	1 cut(s)	1972
LmnI	GCTCC	7 cut(s)	145, 153, 263, 367, 627, 1198, 1441
Lsp1109I	GCAGC	5 cut(s)	53, 904, 924, 1744, 2077
MaeI	CTAG	2 cut(s)	309, 1577
MaeII	ACGT	2 cut(s)	424, 637
MaeIII	GTNAC	5 cut(s)	443, 1033, 1219, 1852, 2233
MfeI	CAATTG	1 cut(s)	742
MflI	RGATCY	2 cut(s)	335, 385
MhlI	GDGCHC	8 cut(s)	91, 150, 282, 1077, 1115, 1195, 2002, 2361
MlyI	GAGTC	4 cut(s)	89, 732, 1873, 2249
MmeI	TCCRAC	1 cut(s)	123
Mph1103I	ATGCAT	3 cut(s)	84, 1648, 1896
MroI	TCCGGA	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	53
MslI	CAYNNNNRTG	1 cut(s)	554
MspA1I	CMGCKG	2 cut(s)	44, 2305
MspCI	CTTAAG	1 cut(s)	436
MspI	CCGG	4 cut(s)	54, 151, 598, 2127
MspR9I	CCNGG	4 cut(s)	598, 599, 1256, 1619
MunI	CAATTG	1 cut(s)	742
Mva1269I	GAATGC	1 cut(s)	408
MvaI	CCWGG	2 cut(s)	1256, 1619
MvnI	CGCG	2 cut(s)	252, 1459
MwoI	GCNNNNNNNGC	8 cut(s)	50, 145, 169, 286, 1757, 1991, 2090, 2291
NaeI	GCCGGC	1 cut(s)	55
NciI	CCSGG	2 cut(s)	598, 599
NdeI	CATATG	2 cut(s)	1511, 1789
NgoMIV	GCCGGC	1 cut(s)	53
NlaIII	CATG	9 cut(s)	82, 665, 1018, 1073, 1291, 1334, 1898, 2035, 2373
NlaIV	GGNNCC	4 cut(s)	591, 841, 1779, 2189
NmeAIII	GCCGAG	3 cut(s)	226, 2006, 2237
NmuCI	GTSAC	2 cut(s)	1219, 1852
NsiI	ATGCAT	3 cut(s)	84, 1648, 1896
NspI	RCATGY	4 cut(s)	82, 665, 1018, 1898
NspV	TTCGAA	1 cut(s)	1841
PctI	GAATGC	1 cut(s)	408
PdiI	GCCGGC	1 cut(s)	55
PfeI	GAWTC	5 cut(s)	48, 415, 1487, 1627, 1838
PflFI	GACNNNGTC	1 cut(s)	512
PflMI	CCANNNNNTGG	2 cut(s)	895, 1108
PfoI	TCCNGGA	1 cut(s)	1254
PinAI	ACCGGT	1 cut(s)	2126
PkrI	GCNGC	6 cut(s)	43, 919, 939, 1759, 2092, 2336
PleI	GAGTC	4 cut(s)	89, 731, 1873, 2249
PpsI	GAGTC	4 cut(s)	89, 731, 1873, 2249
Ppu21I	YACGTR	2 cut(s)	425, 638
PpuMI	RGGWCCY	1 cut(s)	590
PshBI	ATTAAT	1 cut(s)	1370
PsiI	TTATAA	1 cut(s)	1301
Psp124BI	GAGCTC	2 cut(s)	150, 282
Psp5II	RGGWCCY	1 cut(s)	590
Psp6I	CCWGG	2 cut(s)	1254, 1617
PspFI	CCCAGC	1 cut(s)	2094
PspGI	CCWGG	2 cut(s)	1254, 1617
PspN4I	GGNNCC	4 cut(s)	591, 841, 1779, 2189
PspPI	GGNCC	4 cut(s)	153, 590, 1400, 1544
PspPPI	RGGWCCY	1 cut(s)	590
PsrI	GAACNNNNNNTAC	6 cut(s)	1713, 1745, 2113, 2145, 2300, 2332
PstNI	CAGNNNCTG	2 cut(s)	1718, 1757
PsuI	RGATCY	2 cut(s)	335, 385
PsyI	GACNNNGTC	1 cut(s)	512
PvuII	CAGCTG	1 cut(s)	44
RseI	CAYNNNNRTG	1 cut(s)	554
SacI	GAGCTC	2 cut(s)	150, 282
SatI	GCNGC	6 cut(s)	42, 918, 938, 1758, 2091, 2335
Sau96I	GGNCC	4 cut(s)	153, 590, 1400, 1544
ScaI	AGTACT	1 cut(s)	179
SchI	GAGTC	4 cut(s)	89, 732, 1873, 2249
ScrFI	CCNGG	4 cut(s)	598, 599, 1256, 1619
SduI	GDGCHC	8 cut(s)	91, 150, 282, 1077, 1115, 1195, 2002, 2361
SfuI	TTCGAA	1 cut(s)	1841
SinI	GGWCC	3 cut(s)	153, 590, 1544
SmaI	CCCGGG	1 cut(s)	599
SmiMI	CAYNNNNRTG	1 cut(s)	554
SmlI	CTYRAG	4 cut(s)	119, 436, 453, 1199
SmoI	CTYRAG	4 cut(s)	119, 436, 453, 1199
SsiI	CCGC	7 cut(s)	170, 196, 252, 574, 613, 2305, 2334
SspMI	CTAG	2 cut(s)	309, 1577
SstI	GAGCTC	2 cut(s)	150, 282
StyD4I	CCNGG	4 cut(s)	596, 597, 1254, 1617
TaiI	ACGT	2 cut(s)	427, 640
TaqI	TCGA	3 cut(s)	267, 1084, 1841
TatI	WGTACW	5 cut(s)	177, 326, 699, 926, 1514
TauI	GCSGC	1 cut(s)	2337
TfiI	GAWTC	5 cut(s)	48, 415, 1487, 1627, 1838
TscAI	CASTG	3 cut(s)	517, 556, 1982
TseFI	GTSAC	2 cut(s)	1219, 1852
TseI	GCWGC	5 cut(s)	41, 917, 937, 1757, 2090
Tsp45I	GTSAC	2 cut(s)	1219, 1852
TspDTI	ATGAA	8 cut(s)	39, 510, 572, 1035, 1331, 1437, 1853, 2048
TspGWI	ACGGA	1 cut(s)	27
TspMI	CCCGGG	1 cut(s)	597
TspRI	CASTG	3 cut(s)	517, 556, 1982
Tth111I	GACNNNGTC	1 cut(s)	512
Van91I	CCANNNNNTGG	2 cut(s)	895, 1108
Vha464I	CTTAAG	1 cut(s)	436
VneI	GTGCAC	2 cut(s)	1073, 1998
VpaK11BI	GGWCC	3 cut(s)	153, 590, 1544
VspI	ATTAAT	1 cut(s)	1370
XapI	RAATTY	7 cut(s)	544, 1058, 1146, 1259, 1447, 1666, 1830
XceI	RCATGY	4 cut(s)	82, 665, 1018, 1898
XmaI	CCCGGG	1 cut(s)	597
XspI	CTAG	2 cut(s)	309, 1577
ZrmI	AGTACT	1 cut(s)	179
Zsp2I	ATGCAT	3 cut(s)	84, 1648, 1896

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.