MD02G1157700.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015024
Physical Location & Seq
Reverse (-)
13186102 .. 13188365
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1157700.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGATGAACTGCCGGGGTCGTTGGGAACCAGCGCCAGCTTGGCTCTGCGATTCGGGCAGACCATCTTCTCCACTGCCTCCCTTCTCTTCATGTGCTTGGACGTCGAGTTCTACAGCTACACCGCCTTCTGCTATTTGGTGACAGTAATGGGTTTGGTAGTTCCATGGAGCATGACGTTGATGATCGTGGATGTCTACTCTGTCTTCTTTAGATATTTGCCTCGTCGACCAAGAATAACTACAATAATTATTTTAGGAGACTTGGCGTTGTCATATCTCTCACTCGCTGCAGCTTGCTCAACGGCTAGTACCACATATCTCCTACTTCATCTCGGTAGATCCTACTGCCCTGCCAAGTTATGTACTAGATATCAGTTATCTGCTGCAATGGCTTTCCTTTCTTGGTTTCTTTCACTAGGTTCCTCTCTATTCAATCTTTGGCTTCTTCCTTCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

153

Amino Acids

16.91

Weight (kDa)

8.31

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 137 8.4e-25 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50810 AT3G50810 AT3G50810 AT4G37235
fragaria_vesca FvH4_1g14420
malus_domestica MD02G1157700.v1.1 MD15G1272400.v1.1
prunus_persica Prupe.7G146000_v2.0.a1
pyrus_communis pycom15g23600
rosa_chinensis RchiOBHm_Chr2g0103141
rosa_roxburghii Rroxscaffold_2G00139890
rosa_rugosa Rorug02G0112100
rosa_samantha Rh2BG167600 Rh2CG167900 Rh2DG166500
rosa_wichuraiana Rw2G012610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
AccI GTMKAC 2 cut(s) 195, 226
AciI CCGC 1 cut(s) 123
AclWI GGATC 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 2 cut(s) 310, 364
AgsI TTSAA 1 cut(s) 433
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 3 cut(s) 39, 117, 293
AluI AGCT 3 cut(s) 39, 117, 293
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 1 cut(s) 333
ApeKI GCWGC 3 cut(s) 287, 290, 383
AspLEI GCGC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 196
BbvI GCAGC 3 cut(s) 274, 302, 370
BccI CCATC 1 cut(s) 71
BceAI ACGGC 1 cut(s) 318
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 3 cut(s) 306, 366, 416
BfmI CTRYAG 2 cut(s) 112, 288
BfoI RGCGCY 1 cut(s) 36
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 288, 291, 384
BlsI GCNGC 3 cut(s) 289, 292, 385
Bme1390I CCNGG 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 28, 421
BmrFI CCNGG 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 14, 164
Bse3DI GCAATG 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 14, 164
BseGI GGATG 2 cut(s) 10, 196
BseMI GCAATG 1 cut(s) 393
BseXI GCAGC 3 cut(s) 274, 302, 370
BsiSI CCGG 1 cut(s) 14
BsmAI GTCTC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 183, 338
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 28, 421
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 393
BssECI CCNNGG 2 cut(s) 14, 164
BssMI GATC 2 cut(s) 183, 338
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 37, 295
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 10, 196
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 2 cut(s) 186, 341
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 2 cut(s) 183, 338
BstMWI GCNNNNNNNGC 3 cut(s) 41, 55, 389
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 2 cut(s) 112, 288
BstV1I GCAGC 3 cut(s) 274, 302, 370
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 2 cut(s) 10, 196
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 37, 295
CfoI GCGC 1 cut(s) 35
Csp6I GTAC 2 cut(s) 309, 363
CviAII CATG 3 cut(s) 91, 165, 172
CviJI RGCY 7 cut(s) 39, 44, 117, 293, 305, 392, 442
CviKI_1 RGCY 7 cut(s) 39, 44, 117, 293, 305, 392, 442
CviQI GTAC 2 cut(s) 309, 363
DpnI GATC 2 cut(s) 185, 340
DpnII GATC 2 cut(s) 183, 338
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 164
Eco32I GATATC 1 cut(s) 371
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 3 cut(s) 94, 168, 175
FaiI YATR 6 cut(s) 92, 166, 173, 274, 316, 361
FatI CATG 3 cut(s) 90, 164, 171
FblI GTMKAC 2 cut(s) 195, 226
Fnu4HI GCNGC 3 cut(s) 288, 291, 384
FokI GGATG 2 cut(s) 17, 203
Fsp4HI GCNGC 3 cut(s) 288, 291, 384
FspBI CTAG 3 cut(s) 306, 366, 416
GlaI GCGC 1 cut(s) 34
GluI GCNGC 3 cut(s) 288, 291, 384
HaeII RGCGCY 1 cut(s) 36
HapII CCGG 1 cut(s) 14
HhaI GCGC 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 94, 168, 175
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HincII GTYRAC 1 cut(s) 227
HindII GTYRAC 1 cut(s) 227
HinfI GANTC 1 cut(s) 51
HpaII CCGG 1 cut(s) 14
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 2 cut(s) 196, 227
Hpy8I GTNNAC 2 cut(s) 196, 227
Hpy99I CGWCG 2 cut(s) 107, 228
HpyAV CCTTC 3 cut(s) 92, 136, 459
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 2 cut(s) 102, 176
HpyCH4V TGCA 2 cut(s) 290, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 55, 389
HpySE526I ACGT 2 cut(s) 102, 176
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 94, 168, 175
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 2 cut(s) 183, 338
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 4 cut(s) 27, 43, 49, 363
Lsp1109I GCAGC 3 cut(s) 274, 302, 370
MaeI CTAG 3 cut(s) 306, 366, 416
MaeII ACGT 2 cut(s) 102, 176
MaeIII GTNAC 1 cut(s) 139
MalI GATC 2 cut(s) 185, 340
MboI GATC 2 cut(s) 183, 338
MboII GAAGA 4 cut(s) 58, 79, 196, 437
MflI RGATCY 1 cut(s) 338
MluCI AATT 1 cut(s) 246
MnlI CCTC 3 cut(s) 88, 231, 433
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 41, 55, 389
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 164
NdeII GATC 2 cut(s) 183, 338
NlaIII CATG 3 cut(s) 94, 168, 175
NlaIV GGNNCC 2 cut(s) 28, 421
NmuCI GTSAC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 3 cut(s) 289, 292, 385
PspN4I GGNNCC 2 cut(s) 28, 421
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 2 cut(s) 310, 364
RsaNI GTAC 2 cut(s) 309, 363
SalI GTCGAC 1 cut(s) 225
SatI GCNGC 3 cut(s) 288, 291, 384
Sau3AI GATC 2 cut(s) 183, 338
ScrFI CCNGG 1 cut(s) 15
SetI ASST 6 cut(s) 41, 105, 119, 179, 295, 421
SfcI CTRYAG 2 cut(s) 112, 288
Sse9I AATT 1 cut(s) 246
SsiI CCGC 1 cut(s) 123
SspMI CTAG 3 cut(s) 306, 366, 416
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 2 cut(s) 105, 179
TaqI TCGA 2 cut(s) 105, 226
TasI AATT 1 cut(s) 246
TatI WGTACW 1 cut(s) 362
TfiI GAWTC 1 cut(s) 51
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 3 cut(s) 287, 290, 383
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 3 cut(s) 21, 79, 317
TspRI CASTG 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmiI GTMKAC 2 cut(s) 195, 226
XspI CTAG 3 cut(s) 306, 366, 416
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.