Rh2DG166500

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015024
Physical Location & Seq
Reverse (-)
14509408 .. 14512114
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG166500.1

Sequence Viewer

Length: 459 bp
ATGGACGAACTGCCGGGCTCGTTGGGCACCAGTGCCAGCTTGGCTCTGCGTTTCGGCCAGACCCTATTCTCCACCGCATCTCTTCTCTTTATGTGTTTGGACGTCGAGTTCTACAGCTACACAGCTTTCTGCTATTTGGTAACAGTAATGGGTTTGGTAGTTCCATGGGGCATGACTTTAGTGATAGTGGATGCCTACTCTGTTTTTGTTAGATGCTTACCTCGTCAACCAAGAATAATTATAATCCTCATTGTAGGAGACTTGGCTTTATCTTATCTCTCACTAGCTGCAGCCTGCTCAACAGCTAGTACCACAATGCTCCTACATGATGCTGGTCGATCCTTATGCCCAGCAAAATTATGCACTAGATATCAGTTGTCTGCTGCAATGGCTTTTCTTTCTTGGTTTCTCTCTATGGGTTCTTGTCTATTCAATCTTTGGCTTCTTCCTTCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

152

Amino Acids

16.6

Weight (kDa)

6.5

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 137 7.4e-25 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50810 AT3G50810 AT3G50810 AT4G37235
fragaria_vesca FvH4_1g14420
malus_domestica MD02G1157700.v1.1 MD15G1272400.v1.1
prunus_persica Prupe.7G146000_v2.0.a1
pyrus_communis pycom15g23600
rosa_chinensis RchiOBHm_Chr2g0103141
rosa_roxburghii Rroxscaffold_2G00139890
rosa_rugosa Rorug02G0112100
rosa_samantha Rh2BG167600 Rh2CG167900 Rh2DG166500
rosa_wichuraiana Rw2G012610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 242, 457
AatII GACGTC 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 26
AciI CCGC 1 cut(s) 75
AclWI GGATC 1 cut(s) 333
AcoI YGGCCR 1 cut(s) 55
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 310
AgsI TTSAA 1 cut(s) 433
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 5 cut(s) 39, 117, 125, 287, 305
AluI AGCT 5 cut(s) 39, 117, 125, 287, 305
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 1 cut(s) 333
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 3 cut(s) 287, 290, 383
AsuC2I CCSGG 1 cut(s) 15
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 274, 302, 370
BcgI CGANNNNNNTGC 2 cut(s) 327, 361
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 3 cut(s) 284, 306, 366
BfmI CTRYAG 2 cut(s) 112, 288
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 288, 291, 384
BlsI GCNGC 3 cut(s) 289, 292, 385
Bme1390I CCNGG 1 cut(s) 15
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 4 cut(s) 86, 181, 203, 319
BpuMI CCSGG 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 164
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 393
BseNI ACTGG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 3 cut(s) 274, 302, 370
BseYI CCCAGC 1 cut(s) 349
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 26
BsiSI CCGG 1 cut(s) 14
BsmAI GTCTC 1 cut(s) 252
BsnI GGCC 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 20, 29
Bsp143I GATC 1 cut(s) 338
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 1 cut(s) 75
BspANI GGCC 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 393
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 1 cut(s) 338
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 37, 295
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 1 cut(s) 341
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 1 cut(s) 338
BstMWI GCNNNNNNNGC 3 cut(s) 24, 41, 389
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 2 cut(s) 112, 288
BstSLI GKGCMC 1 cut(s) 29
BstV1I GCAGC 3 cut(s) 274, 302, 370
BsuRI GGCC 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 37, 295
Csp6I GTAC 1 cut(s) 309
CviAII CATG 3 cut(s) 165, 172, 326
CviQI GTAC 1 cut(s) 309
DpnI GATC 1 cut(s) 340
DpnII GATC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 371
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 164
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 3 cut(s) 168, 175, 329
FaiI YATR 9 cut(s) 92, 166, 173, 242, 327, 346, 361, 416, 457
FatI CATG 3 cut(s) 164, 171, 325
Fnu4HI GCNGC 3 cut(s) 288, 291, 384
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 288, 291, 384
FspBI CTAG 3 cut(s) 284, 306, 366
GluI GCNGC 3 cut(s) 288, 291, 384
GsaI CCCAGC 1 cut(s) 353
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 168, 175, 329
HincII GTYRAC 1 cut(s) 227
HindII GTYRAC 1 cut(s) 227
HpaII CCGG 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 227
Hpy8I GTNNAC 1 cut(s) 227
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 459
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 3 cut(s) 290, 363, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 41, 389
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 168, 175, 329
Kzo9I GATC 1 cut(s) 338
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 7 cut(s) 27, 43, 49, 71, 307, 318, 363
Lsp1109I GCAGC 3 cut(s) 274, 302, 370
LweI GCATC 4 cut(s) 86, 181, 203, 319
MaeI CTAG 3 cut(s) 284, 306, 366
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 139
MalI GATC 1 cut(s) 340
MboI GATC 1 cut(s) 338
MboII GAAGA 2 cut(s) 74, 437
MhlI GDGCHC 2 cut(s) 20, 29
MluCI AATT 2 cut(s) 237, 356
MnlI CCTC 2 cut(s) 231, 257
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 24, 41, 389
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 164
NdeII GATC 1 cut(s) 338
NlaIII CATG 3 cut(s) 168, 175, 329
NlaIV GGNNCC 1 cut(s) 28
PkrI GCNGC 3 cut(s) 289, 292, 385
PsiI TTATAA 2 cut(s) 242, 457
PspFI CCCAGC 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 292
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
SatI GCNGC 3 cut(s) 288, 291, 384
Sau3AI GATC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 2 cut(s) 20, 29
SetI ASST 7 cut(s) 41, 105, 119, 127, 223, 289, 307
SfaNI GCATC 4 cut(s) 86, 181, 203, 319
SfcI CTRYAG 2 cut(s) 112, 288
Sse9I AATT 2 cut(s) 237, 356
SsiI CCGC 1 cut(s) 75
SspMI CTAG 3 cut(s) 284, 306, 366
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 105, 337
TasI AATT 2 cut(s) 237, 356
TscAI CASTG 1 cut(s) 37
TseI GCWGC 3 cut(s) 287, 290, 383
TspRI CASTG 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 3 cut(s) 284, 306, 366
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.