MD15G1272400.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015024
Physical Location & Seq
Reverse (-)
23789009 .. 23791634
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1272400.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGATGAACTGCCGGGGTCGTTGGGAACCAGTGCCAGCTTGGCTCTGCGATTCGGGCAGACCATCTTCTCCACTGCCTCTCTTCTCTTCATGTGCTTGGACGTCGAGTTCTACAGCTACACCGCCTTCTGCTATTTGGTGACGGTAATGGGTTTGGTAGTTCCGTGGAGCATGACTCTGGTGATCGTTGATGTCTACTCTGTCTTCTTTAGATGTTTGCATCATCAACCAAGAATAATTATAATAATTATTTTAGGAGACTTGGCGTTGTCGTATCTCTCACTCGCTGCAGCTTGCACGACCGCTAGTACCACAAATCTCCTACTGTATCTCGACGGATCCTACTGCCCCGCCAAGTTATGTACTAGATATCAGTTATCTGCTGCCATGGCTTTCCTGTCTTGGTTTCTTTCACTAGGTTCCTCTCTGTTCAATCTCTGGCTTCTTGCTTCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

153

Amino Acids

16.76

Weight (kDa)

5.39

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 137 1.6e-24 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50810 AT3G50810 AT3G50810 AT4G37235
fragaria_vesca FvH4_1g14420
malus_domestica MD02G1157700.v1.1 MD15G1272400.v1.1
prunus_persica Prupe.7G146000_v2.0.a1
pyrus_communis pycom15g23600
rosa_chinensis RchiOBHm_Chr2g0103141
rosa_roxburghii Rroxscaffold_2G00139890
rosa_rugosa Rorug02G0112100
rosa_samantha Rh2BG167600 Rh2CG167900 Rh2DG166500
rosa_wichuraiana Rw2G012610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
AatII GACGTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 195
AciI CCGC 3 cut(s) 123, 303, 351
AclWI GGATC 2 cut(s) 333, 346
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 2 cut(s) 310, 364
AgsI TTSAA 1 cut(s) 433
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 3 cut(s) 39, 117, 293
AluI AGCT 3 cut(s) 39, 117, 293
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 2 cut(s) 333, 346
ApeKI GCWGC 3 cut(s) 287, 290, 383
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 2 cut(s) 151, 193
BamHI GGATCC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 196
BbvI GCAGC 3 cut(s) 274, 302, 370
BccI CCATC 1 cut(s) 71
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 3 cut(s) 306, 366, 416
BfmI CTRYAG 2 cut(s) 112, 288
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 288, 291, 384
BlsI GCNGC 3 cut(s) 289, 292, 385
Bme1390I CCNGG 1 cut(s) 15
BmiI GGNNCC 3 cut(s) 28, 340, 421
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 196
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BpuMI CCSGG 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 14, 164, 387
Bse1I ACTGG 1 cut(s) 30
BseDI CCNNGG 3 cut(s) 14, 164, 387
BseGI GGATG 1 cut(s) 10
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 3 cut(s) 274, 302, 370
Bsh1285I CGRYCG 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 14
BsmAI GTCTC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 183, 338
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 3 cut(s) 123, 303, 351
BspLI GGNNCC 3 cut(s) 28, 340, 421
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 2 cut(s) 333, 346
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 3 cut(s) 14, 164, 387
BssMI GATC 2 cut(s) 183, 338
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 145, 327
Bst6I CTCTTC 2 cut(s) 87, 92
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 37, 295
BstDSI CCRYGG 2 cut(s) 164, 387
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 2 cut(s) 186, 341
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 2 cut(s) 183, 338
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 3 cut(s) 41, 55, 389
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 2 cut(s) 112, 288
BstV1I GCAGC 3 cut(s) 274, 302, 370
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BtgI CCRYGG 2 cut(s) 164, 387
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 37, 72
Cac8I GCNNGC 2 cut(s) 37, 295
Csp6I GTAC 2 cut(s) 309, 363
CviAII CATG 3 cut(s) 91, 172, 388
CviJI RGCY 6 cut(s) 39, 44, 117, 293, 392, 442
CviKI_1 RGCY 6 cut(s) 39, 44, 117, 293, 392, 442
CviQI GTAC 2 cut(s) 309, 363
DpnI GATC 2 cut(s) 185, 340
DpnII GATC 2 cut(s) 183, 338
Eam1104I CTCTTC 2 cut(s) 87, 92
EarI CTCTTC 2 cut(s) 87, 92
Eco130I CCWWGG 1 cut(s) 387
Eco32I GATATC 1 cut(s) 371
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 3 cut(s) 94, 175, 391
FaiI YATR 5 cut(s) 92, 173, 242, 361, 389
FatI CATG 3 cut(s) 90, 171, 387
FauI CCCGC 1 cut(s) 358
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 288, 291, 384
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 288, 291, 384
FspBI CTAG 3 cut(s) 306, 366, 416
GluI GCNGC 3 cut(s) 288, 291, 384
HapII CCGG 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 94, 175, 391
HinfI GANTC 2 cut(s) 51, 175
HpaII CCGG 1 cut(s) 14
HphI GGTGA 2 cut(s) 151, 193
Hpy166II GTNNAC 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 2 cut(s) 107, 338
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 2 cut(s) 145, 327
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 3 cut(s) 220, 290, 297
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 55, 389
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 94, 175, 391
Kzo9I GATC 2 cut(s) 183, 338
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 6 cut(s) 27, 43, 49, 164, 410, 424
Lsp1109I GCAGC 3 cut(s) 274, 302, 370
LweI GCATC 1 cut(s) 229
MaeI CTAG 3 cut(s) 306, 366, 416
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 139
MalI GATC 2 cut(s) 185, 340
MboI GATC 2 cut(s) 183, 338
MboII GAAGA 4 cut(s) 58, 74, 79, 196
MflI RGATCY 1 cut(s) 338
MluCI AATT 2 cut(s) 237, 246
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 2 cut(s) 88, 433
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 41, 55, 389
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 387
NdeII GATC 2 cut(s) 183, 338
NlaIII CATG 3 cut(s) 94, 175, 391
NlaIV GGNNCC 3 cut(s) 28, 340, 421
NmuCI GTSAC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 3 cut(s) 289, 292, 385
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PsiI TTATAA 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 28, 340, 421
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 2 cut(s) 310, 364
RsaNI GTAC 2 cut(s) 309, 363
SatI GCNGC 3 cut(s) 288, 291, 384
Sau3AI GATC 2 cut(s) 183, 338
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 1 cut(s) 15
SetI ASST 5 cut(s) 41, 105, 119, 295, 421
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 2 cut(s) 112, 288
Sse9I AATT 2 cut(s) 237, 246
SsiI CCGC 3 cut(s) 123, 303, 351
SspMI CTAG 3 cut(s) 306, 366, 416
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 145, 327
TaiI ACGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 105, 333
TasI AATT 2 cut(s) 237, 246
TatI WGTACW 1 cut(s) 362
TfiI GAWTC 1 cut(s) 51
TscAI CASTG 2 cut(s) 37, 79
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 3 cut(s) 287, 290, 383
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 21, 79
TspGWI ACGGA 2 cut(s) 153, 351
TspRI CASTG 2 cut(s) 37, 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 3 cut(s) 306, 366, 416
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.