Rorug02G0112100

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015024
Physical Location & Seq
Forward (+)
9593462 .. 9596098
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0112100.1

Sequence Viewer

Length: 1107 bp
ATGGCTCGAAGAACGATACCGATCCTCCGCCACCTCCTAACAAAGCCCCGACCCGGCGTCGCGGCCCAAACCCGATCGGTCACCTACATGCCCCGACCCGGCGACGGAGCTCCGCGCGCCGTGACTCTAATCCCCGGCGACGGAATCGGACCGCTCGTCACGAACGCCGTGGAGCAGGTGATGGAGGCGATGCACGCGCCGGTCTACTTCGAGAAGTACGAGGTCACCGGCGACATGCCGAGGGTGCCGGAGGAGGTGATCGAGTCGATCAGGAAGAACAAGGTGTGCCTGAAAGGCGGGCTGGCGACGCCGATGGGCGGCGGCGTCAGCTCACTGAACATGCAGCTCCGGCGAGAGCTCGATCTCTATGCCTCGCTCGTCAACTGCTTCAATCTGCGTGGCTTGCAGACCAAGCATGACAACGTCGACATTGTGGTGATCAGGGAGAACACCGAGGGCGAGTACTCGGGGCTCGAGCACGAGGTCATTCCTGGAGTCGTTGAAAGCCTTAAGGTGATCACAAAGTTCTGTTCAGAGCGTATTGCTAGATATGCTTTTGAGTATGCTTACCTGAACAACAGAAAGAAGGTGACTGCTGTGCACAAAGCAAACATTATGAAGCTTGCAGATGGTCTGTTCTTAGAGTCTTGTCGTGAGGTGGCTACAAATTATCCAGGGATCACTTACAATGAAGTAATTGTTGACAACTGCTGCATGCAAGTTGTTGCAAGGCCCGAGCAATTTGATGTCATGGTGACACCCAATCTTTATGGTAACCTAGTTGCAAATACAGCCGCTGGTATTGCTGGAGGCACTGGTGTCATGCCAGGAGGAAATGTGGGGGCAGATCATGCTATTTTTGAGCAAGGTGCTTCAGCAGGAAATGTGGGGAATCAGAAAATATTGGATCAAAAGACAGCAAACCCAGTAGCCTTGCTTCTCTCCTCTGCCATGATGTTGAGACATTTGCAATTTCCTTCCTTCTCTGATCGATTAGAAAATGCTGTCGAAAGAGTAATCTTAGAGGGCAAGTTCAGGACAAAGGACCTTGGAGGACATAGCACTACCCAAGAGGTTGTTGATGCGGTCATTGCTGCCTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

368

Amino Acids

40.04

Weight (kDa)

6.47

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 41 - 363 4e-79 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50810 AT3G50810 AT3G50810 AT4G37235
fragaria_vesca FvH4_1g14420
malus_domestica MD02G1157700.v1.1 MD15G1272400.v1.1
prunus_persica Prupe.7G146000_v2.0.a1
pyrus_communis pycom15g23600
rosa_chinensis RchiOBHm_Chr2g0103141
rosa_roxburghii Rroxscaffold_2G00139890
rosa_rugosa Rorug02G0112100
rosa_samantha Rh2BG167600 Rh2CG167900 Rh2DG166500
rosa_wichuraiana Rw2G012610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AccB1I GGYRCC 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 154
AccI GTMKAC 2 cut(s) 204, 426
AccII CGCG 4 cut(s) 62, 115, 117, 197
AciI CCGC 9 cut(s) 28, 62, 113, 152, 297, 318, 321, 795, 1085
AclWI GGATC 3 cut(s) 16, 686, 915
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 3 cut(s) 57, 308, 324
AfaI GTAC 2 cut(s) 218, 464
AfiI CCNNNNNNNGG 5 cut(s) 53, 98, 104, 140, 317
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 2 cut(s) 391, 503
AhdI GACNNNNNGTC 2 cut(s) 56, 155
AjnI CCWGG 3 cut(s) 490, 673, 826
AluBI AGCT 5 cut(s) 110, 330, 346, 358, 622
AluI AGCT 5 cut(s) 110, 330, 346, 358, 622
Alw21I GWGCWC 4 cut(s) 112, 360, 480, 603
Alw26I GTCTC 1 cut(s) 955
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 3 cut(s) 16, 686, 915
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 3 cut(s) 466, 473, 734
AoxI GGCC 2 cut(s) 63, 731
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 3 cut(s) 343, 711, 1094
AspLEI GCGC 3 cut(s) 117, 119, 199
AspS9I GGNCC 4 cut(s) 64, 149, 732, 1045
AsuC2I CCSGG 3 cut(s) 54, 99, 135
AsuHPI GGTGA 8 cut(s) 73, 190, 217, 268, 448, 526, 601, 766
AvaI CYCGRG 3 cut(s) 466, 473, 734
AvaII GGWCC 2 cut(s) 149, 1045
BaeGI GKGCMC 1 cut(s) 603
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 3 cut(s) 112, 360, 474
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BauI CACGAG 1 cut(s) 479
Bbv12I GWGCWC 4 cut(s) 112, 360, 480, 603
BbvI GCAGC 3 cut(s) 355, 698, 1081
BccI CCATC 3 cut(s) 175, 307, 623
BceAI ACGGC 2 cut(s) 104, 152
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 3 cut(s) 492, 675, 828
BclI TGATCA 2 cut(s) 438, 516
BcnI CCSGG 3 cut(s) 54, 99, 135
BcoDI GTCTC 1 cut(s) 955
BfaI CTAG 2 cut(s) 546, 779
BfrI CTTAAG 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 294
BisI GCNGC 7 cut(s) 63, 319, 322, 344, 712, 795, 1095
BlsI GCNGC 7 cut(s) 64, 320, 323, 345, 713, 796, 1096
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 6 cut(s) 54, 99, 135, 492, 675, 828
Bme18I GGWCC 2 cut(s) 149, 1045
BmeRI GACNNNNNGTC 2 cut(s) 56, 155
BmeT110I CYCGRG 3 cut(s) 466, 473, 734
BmgT120I GGNCC 4 cut(s) 64, 149, 732, 1045
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 6 cut(s) 54, 99, 135, 492, 675, 828
BmrI ACTGGG 1 cut(s) 920
BmsI GCATC 2 cut(s) 180, 1072
BmuI ACTGGG 1 cut(s) 920
BpmI CTGGAG 2 cut(s) 513, 828
BpuMI CCSGG 3 cut(s) 54, 99, 135
Bsa29I ATCGAT 1 cut(s) 991
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 3 cut(s) 57, 308, 324
BsaJI CCNNGG 6 cut(s) 133, 168, 239, 453, 674, 1048
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 822, 852
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 98, 104, 140, 317
Bse118I RCCGGY 2 cut(s) 199, 227
Bse1I ACTGG 2 cut(s) 820, 926
Bse3DI GCAATG 1 cut(s) 1089
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 3 cut(s) 492, 675, 828
BseCI ATCGAT 1 cut(s) 991
BseDI CCNNGG 6 cut(s) 133, 168, 239, 453, 674, 1048
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 5 cut(s) 53, 98, 104, 140, 317
BseMI GCAATG 1 cut(s) 1089
BseNI ACTGG 2 cut(s) 820, 926
BsePI GCGCGC 1 cut(s) 115
BseRI GAGGAG 2 cut(s) 266, 934
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 3 cut(s) 355, 698, 1081
Bsh1236I CGCG 4 cut(s) 62, 115, 117, 197
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 65, 733
BshNI GGYRCC 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 991
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 4 cut(s) 112, 360, 480, 603
BsiHKCI CYCGRG 3 cut(s) 466, 473, 734
BsiSI CCGG 7 cut(s) 54, 99, 135, 200, 228, 248, 349
BslI CCNNNNNNNGG 5 cut(s) 53, 98, 104, 140, 317
BsmAI GTCTC 1 cut(s) 955
BsnI GGCC 2 cut(s) 65, 733
BsoBI CYCGRG 3 cut(s) 466, 473, 734
Bsp1286I GDGCHC 5 cut(s) 112, 360, 474, 480, 603
BspACI CCGC 9 cut(s) 28, 62, 113, 152, 297, 318, 321, 795, 1085
BspANI GGCC 2 cut(s) 65, 733
BspDI ATCGAT 1 cut(s) 991
BspFNI CGCG 4 cut(s) 62, 115, 117, 197
BspLI GGNNCC 1 cut(s) 246
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 3 cut(s) 16, 686, 915
BspT107I GGYRCC 1 cut(s) 244
BspTI CTTAAG 1 cut(s) 509
BsrBI CCGCTC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 1089
BsrFI RCCGGY 2 cut(s) 199, 227
BsrI ACTGG 2 cut(s) 820, 926
BssAI RCCGGY 2 cut(s) 199, 227
BssECI CCNNGG 6 cut(s) 133, 168, 239, 453, 674, 1048
BssHII GCGCGC 1 cut(s) 115
BssNI GRCGYC 3 cut(s) 57, 308, 324
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 1048
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 3 cut(s) 492, 675, 828
BstACI GRCGYC 3 cut(s) 57, 308, 324
BstAFI CTTAAG 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 851
BstC8I GCNNGC 7 cut(s) 117, 195, 299, 303, 404, 624, 716
BstDEI CTNAG 3 cut(s) 640, 1021, 1099
BstDSI CCRYGG 1 cut(s) 168
BstEII GGTNACC 3 cut(s) 79, 223, 773
BstFNI CGCG 4 cut(s) 62, 115, 117, 197
BstHHI GCGC 3 cut(s) 117, 119, 199
BstMAI GTCTC 1 cut(s) 955
BstMCI CGRYCG 1 cut(s) 77
BstNI CCWGG 3 cut(s) 492, 675, 828
BstNSI RCATGY 4 cut(s) 91, 238, 343, 718
BstPI GGTNACC 3 cut(s) 79, 223, 773
BstSCI CCNGG 6 cut(s) 52, 97, 133, 490, 673, 826
BstSLI GKGCMC 1 cut(s) 603
BstUI CGCG 4 cut(s) 62, 115, 117, 197
BstV1I GCAGC 3 cut(s) 355, 698, 1081
Bsu15I ATCGAT 1 cut(s) 991
BsuRI GGCC 2 cut(s) 65, 733
BsuTUI ATCGAT 1 cut(s) 991
BtgI CCRYGG 1 cut(s) 168
BtgZI GCGATG 1 cut(s) 203
BtsIMutI CAGTG 2 cut(s) 332, 813
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 7 cut(s) 117, 195, 299, 303, 404, 624, 716
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 3 cut(s) 117, 119, 199
Cfr10I RCCGGY 2 cut(s) 199, 227
Cfr13I GGNCC 4 cut(s) 64, 149, 732, 1045
ClaI ATCGAT 1 cut(s) 991
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 3 cut(s) 46, 313, 316
Csp6I GTAC 2 cut(s) 217, 463
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CspI CGGWCCG 1 cut(s) 149
CviAII CATG 9 cut(s) 88, 235, 340, 416, 715, 751, 823, 851, 952
CviQI GTAC 2 cut(s) 217, 463
DdeI CTNAG 3 cut(s) 640, 1021, 1099
DriI GACNNNNNGTC 2 cut(s) 56, 155
Eam1105I GACNNNNNGTC 2 cut(s) 56, 155
EciI GGCGGA 1 cut(s) 17
Ecl136II GAGCTC 2 cut(s) 110, 358
Eco130I CCWWGG 1 cut(s) 1048
Eco24I GRGCYC 3 cut(s) 112, 360, 474
Eco47I GGWCC 2 cut(s) 149, 1045
Eco53kI GAGCTC 2 cut(s) 110, 358
Eco57I CTGAAG 1 cut(s) 858
Eco88I CYCGRG 3 cut(s) 466, 473, 734
Eco91I GGTNACC 3 cut(s) 79, 223, 773
EcoICRI GAGCTC 2 cut(s) 110, 358
EcoO109I RGGNCCY 1 cut(s) 1045
EcoO65I GGTNACC 3 cut(s) 79, 223, 773
EcoRII CCWGG 3 cut(s) 490, 673, 826
EcoT14I CCWWGG 1 cut(s) 1048
EcoT38I GRGCYC 3 cut(s) 112, 360, 474
ErhI CCWWGG 1 cut(s) 1048
FaeI CATG 9 cut(s) 91, 238, 343, 419, 718, 754, 826, 854, 955
FatI CATG 9 cut(s) 87, 234, 339, 415, 714, 750, 822, 850, 951
FauI CCCGC 1 cut(s) 290
FbaI TGATCA 2 cut(s) 438, 516
FblI GTMKAC 2 cut(s) 204, 426
Fnu4HI GCNGC 7 cut(s) 63, 319, 322, 344, 712, 795, 1095
FriOI GRGCYC 3 cut(s) 112, 360, 474
Fsp4HI GCNGC 7 cut(s) 63, 319, 322, 344, 712, 795, 1095
FspBI CTAG 2 cut(s) 546, 779
GlaI GCGC 3 cut(s) 116, 118, 198
GluI GCNGC 7 cut(s) 63, 319, 322, 344, 712, 795, 1095
GsuI CTGGAG 2 cut(s) 513, 828
HaeIII GGCC 2 cut(s) 65, 733
HapII CCGG 7 cut(s) 54, 99, 135, 200, 228, 248, 349
HgaI GACGC 3 cut(s) 46, 313, 316
HhaI GCGC 3 cut(s) 117, 119, 199
Hin1I GRCGYC 3 cut(s) 57, 308, 324
Hin1II CATG 9 cut(s) 91, 238, 343, 419, 718, 754, 826, 854, 955
Hin6I GCGC 3 cut(s) 115, 117, 197
HinP1I GCGC 3 cut(s) 115, 117, 197
HincII GTYRAC 3 cut(s) 382, 427, 703
HindII GTYRAC 3 cut(s) 382, 427, 703
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 6 cut(s) 124, 144, 263, 495, 644, 892
HpaII CCGG 7 cut(s) 54, 99, 135, 200, 228, 248, 349
HphI GGTGA 8 cut(s) 73, 190, 217, 268, 448, 526, 601, 766
Hpy166II GTNNAC 5 cut(s) 205, 382, 427, 601, 703
Hpy188I TCNGA 4 cut(s) 149, 535, 897, 988
Hpy188III TCNNGA 5 cut(s) 160, 211, 271, 653, 1036
Hpy8I GTNNAC 5 cut(s) 205, 382, 427, 601, 703
Hpy99I CGWCG 5 cut(s) 62, 107, 143, 310, 428
HpyAV CCTTC 3 cut(s) 580, 987, 991
HpyCH4IV ACGT 1 cut(s) 423
HpyF3I CTNAG 3 cut(s) 640, 1021, 1099
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 3 cut(s) 57, 308, 324
Hsp92II CATG 9 cut(s) 91, 238, 343, 419, 718, 754, 826, 854, 955
HspAI GCGC 3 cut(s) 115, 117, 197
Ksp22I TGATCA 2 cut(s) 438, 516
LmnI GCTCC 4 cut(s) 107, 115, 172, 351
Lsp1109I GCAGC 3 cut(s) 355, 698, 1081
LweI GCATC 2 cut(s) 180, 1072
MaeI CTAG 2 cut(s) 546, 779
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 7 cut(s) 79, 121, 157, 223, 589, 754, 773
MbiI CCGCTC 1 cut(s) 154
MboII GAAGA 2 cut(s) 21, 286
MhlI GDGCHC 5 cut(s) 112, 360, 474, 480, 603
MluCI AATT 4 cut(s) 667, 696, 740, 971
MlyI GAGTC 4 cut(s) 118, 272, 504, 653
MseI TTAA 1 cut(s) 510
MslI CAYNNNNRTG 2 cut(s) 86, 434
MspA1I CMGCKG 1 cut(s) 797
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 7 cut(s) 54, 99, 135, 200, 228, 248, 349
MspR9I CCNGG 6 cut(s) 54, 99, 135, 492, 675, 828
MvaI CCWGG 3 cut(s) 492, 675, 828
MvnI CGCG 4 cut(s) 62, 115, 117, 197
NciI CCSGG 3 cut(s) 54, 99, 135
NlaIII CATG 9 cut(s) 91, 238, 343, 419, 718, 754, 826, 854, 955
NlaIV GGNNCC 1 cut(s) 246
NmeAIII GCCGAG 1 cut(s) 264
NmuCI GTSAC 6 cut(s) 79, 121, 157, 223, 589, 754
NspI RCATGY 4 cut(s) 91, 238, 343, 718
PaeI GCATGC 1 cut(s) 718
PaeR7I CTCGAG 1 cut(s) 473
PaqCI CACCTGC 1 cut(s) 166
PauI GCGCGC 1 cut(s) 115
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 216
PfeI GAWTC 2 cut(s) 144, 892
PflFI GACNNNGTC 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 7 cut(s) 64, 320, 323, 345, 713, 796, 1096
Ple19I CGATCG 1 cut(s) 77
PleI GAGTC 4 cut(s) 118, 271, 503, 652
PpsI GAGTC 4 cut(s) 118, 271, 503, 652
PpuMI RGGWCCY 1 cut(s) 1045
Psp124BI GAGCTC 2 cut(s) 112, 360
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 3 cut(s) 490, 673, 826
PspEI GGTNACC 3 cut(s) 79, 223, 773
PspGI CCWGG 3 cut(s) 490, 673, 826
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 4 cut(s) 64, 149, 732, 1045
PspPPI RGGWCCY 1 cut(s) 1045
PspXI VCTCGAGB 1 cut(s) 473
PstNI CAGNNNCTG 1 cut(s) 797
PsyI GACNNNGTC 1 cut(s) 422
PteI GCGCGC 1 cut(s) 115
PvuI CGATCG 1 cut(s) 77
RsaI GTAC 2 cut(s) 218, 464
RsaNI GTAC 2 cut(s) 217, 463
RseI CAYNNNNRTG 2 cut(s) 86, 434
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 2 cut(s) 112, 360
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 7 cut(s) 63, 319, 322, 344, 712, 795, 1095
Sau96I GGNCC 4 cut(s) 64, 149, 732, 1045
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 4 cut(s) 118, 272, 504, 653
ScrFI CCNGG 6 cut(s) 54, 99, 135, 492, 675, 828
SduI GDGCHC 5 cut(s) 112, 360, 474, 480, 603
SfaNI GCATC 2 cut(s) 180, 1072
Sfr274I CTCGAG 1 cut(s) 473
SgrAI CRCCGGYG 1 cut(s) 227
SinI GGWCC 2 cut(s) 149, 1045
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 2 cut(s) 86, 434
SmlI CTYRAG 2 cut(s) 473, 509
SmoI CTYRAG 2 cut(s) 473, 509
SphI GCATGC 1 cut(s) 718
Sse9I AATT 4 cut(s) 667, 696, 740, 971
SsiI CCGC 9 cut(s) 28, 62, 113, 152, 297, 318, 321, 795, 1085
SspI AATATT 1 cut(s) 903
SspMI CTAG 2 cut(s) 546, 779
SstI GAGCTC 2 cut(s) 112, 360
StyD4I CCNGG 6 cut(s) 52, 97, 133, 490, 673, 826
StyI CCWWGG 1 cut(s) 1048
TaiI ACGT 1 cut(s) 426
TaqI TCGA 9 cut(s) 7, 210, 261, 266, 360, 426, 474, 991, 1008
TaqII GACCGA 1 cut(s) 67
TasI AATT 4 cut(s) 667, 696, 740, 971
TatI WGTACW 1 cut(s) 462
TauI GCSGC 4 cut(s) 65, 321, 324, 797
TfiI GAWTC 2 cut(s) 144, 892
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TscAI CASTG 2 cut(s) 339, 820
TseFI GTSAC 6 cut(s) 79, 121, 157, 223, 589, 754
TseI GCWGC 3 cut(s) 343, 711, 1094
Tsp45I GTSAC 6 cut(s) 79, 121, 157, 223, 589, 754
TspDTI ATGAA 2 cut(s) 632, 705
TspGWI ACGGA 2 cut(s) 120, 156
TspRI CASTG 2 cut(s) 339, 820
Tth111I GACNNNGTC 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 509
VneI GTGCAC 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 149, 1045
XceI RCATGY 4 cut(s) 91, 238, 343, 718
XhoI CTCGAG 1 cut(s) 473
XmiI GTMKAC 2 cut(s) 204, 426
XspI CTAG 2 cut(s) 546, 779
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.