MD02G1158500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014059
Physical Location & Seq
Forward (+)
13212818 .. 13213291
474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1158500.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCTGCGAGAGCTTCAGTACTCGCTCTCCACCCAGAGCGCAATGCGCACCGCAGCTCCTCCTCCTCCCCGGCGCACGTTCCGAAGCCTACGCCGCCGAGTGGACTCGCATTATCCGGGCGGAGACAGCTGGCGCTGTCGCTGACGTGGACCGCGGCGATTGCGGCTGTGGGTGCAAAGGAGGCGAAGGCGGAGGACATCGGGCTGTTTGGGCTGAGGAGGAAGGTGAAGGAGGTGGAGAAGGAGGCGGAGGTGATAGTGAAGGAAGGGATTGAGGCGGCGGAGAAGGGACTGGAAACGGCGGAGAAGGGGCTGGAATCGGCGGAGCTGGGGATTGAAACGGCGGAGGAAGGGGTGGAAGCGGCGTTGAGCTTCGGGGGGTTGGCGCAGGCTGGAGCGGTGGCGGGAGCGGAGTTTGTGGGGGTTTTGGTGGCAACTTCGGTTGTGAGTGGGATTCTGGGGCCGGAGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

15.92

Weight (kDa)

5.3

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23670
fragaria_vesca FvH4_1g14510
malus_domestica MD02G1158500.v1.1 MD15G1273300.v1.1
prunus_persica Prupe.7G145300_v2.0.a1
pyrus_communis pycom02g12500
rosa_chinensis RchiOBHm_Chr2g0103251
rosa_laevigata RLG00000017218
rosa_multiflora Rmu_sc0002218.1_g000001 Rmu_sc0006743.1_g000013
rosa_roxburghii Rroxscaffold_2G00139810
rosa_rugosa Rorug02G0112800
rosa_samantha Rh2AG161500 Rh2BG168300 Rh2CG168400 Rh2DG167200
rosa_wichuraiana Rw2G012680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 48
AccBSI CCGCTC 2 cut(s) 398, 410
AccII CGCG 1 cut(s) 155
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 338
AjiI CACGTC 1 cut(s) 147
AluBI AGCT 5 cut(s) 14, 57, 130, 328, 372
AluI AGCT 5 cut(s) 14, 57, 130, 328, 372
Alw26I GTCTC 1 cut(s) 118
AoxI GGCC 1 cut(s) 461
ApeKI GCWGC 2 cut(s) 5, 54
AspLEI GCGC 5 cut(s) 42, 49, 76, 136, 388
AspS9I GGNCC 2 cut(s) 150, 461
AsuC2I CCSGG 2 cut(s) 71, 118
AsuHPI GGTGA 2 cut(s) 238, 265
AvaII GGWCC 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 66
BceAI ACGGC 2 cut(s) 315, 357
BcnI CCSGG 2 cut(s) 71, 118
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 137
BisI GCNGC 7 cut(s) 6, 55, 95, 156, 165, 279, 363
BlsI GCNGC 7 cut(s) 7, 56, 96, 157, 166, 280, 364
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 2 cut(s) 71, 118
Bme18I GGWCC 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 150, 461
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 2 cut(s) 71, 118
BpmI CTGGAG 1 cut(s) 414
Bpu10I CCTNAGC 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 71, 118
BsaJI CCNNGG 2 cut(s) 69, 153
BsaXI ACNNNNNCTCC 4 cut(s) 12, 41, 42, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 49
BseDI CCNNGG 2 cut(s) 69, 153
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 4 cut(s) 49, 52, 55, 232
BseXI GCAGC 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 463
BsiSI CCGG 3 cut(s) 71, 117, 464
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 1 cut(s) 463
BspANI GGCC 1 cut(s) 463
BspCNI CTCAG 1 cut(s) 207
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 462
BsrBI CCGCTC 2 cut(s) 398, 410
BsrDI GCAATG 1 cut(s) 49
BsrI ACTGG 1 cut(s) 297
BssECI CCNNGG 2 cut(s) 69, 153
BstC8I GCNNGC 2 cut(s) 132, 390
BstDEI CTNAG 1 cut(s) 215
BstDSI CCRYGG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 5 cut(s) 42, 49, 76, 136, 388
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 9 cut(s) 11, 46, 94, 127, 161, 164, 173, 182, 211
BstSCI CCNGG 2 cut(s) 69, 116
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 66
BsuRI GGCC 1 cut(s) 463
BtgI CCRYGG 1 cut(s) 153
BtrI CACGTC 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 132, 390
CfoI GCGC 5 cut(s) 42, 49, 76, 136, 388
Cfr13I GGNCC 2 cut(s) 150, 461
Cfr42I CCGCGG 1 cut(s) 156
Csp6I GTAC 1 cut(s) 20
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 1 cut(s) 215
EciI GGCGGA 7 cut(s) 136, 206, 263, 296, 317, 338, 359
Eco47I GGWCC 1 cut(s) 150
FaqI GGGAC 1 cut(s) 303
FauI CCCGC 1 cut(s) 397
Fnu4HI GCNGC 7 cut(s) 6, 55, 95, 156, 165, 279, 363
Fsp4HI GCNGC 7 cut(s) 6, 55, 95, 156, 165, 279, 363
FspAI RTGCGCAY 1 cut(s) 48
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 5 cut(s) 41, 48, 75, 135, 387
GluI GCNGC 7 cut(s) 6, 55, 95, 156, 165, 279, 363
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 1 cut(s) 414
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 1 cut(s) 463
HapII CCGG 3 cut(s) 71, 117, 464
HhaI GCGC 5 cut(s) 42, 49, 76, 136, 388
Hin6I GCGC 5 cut(s) 40, 47, 74, 134, 386
HinP1I GCGC 5 cut(s) 40, 47, 74, 134, 386
HinfI GANTC 3 cut(s) 105, 317, 454
HpaII CCGG 3 cut(s) 71, 117, 464
HphI GGTGA 2 cut(s) 238, 265
Hpy166II GTNNAC 2 cut(s) 104, 150
Hpy188I TCNGA 1 cut(s) 84
Hpy8I GTNNAC 2 cut(s) 104, 150
HpyAV CCTTC 9 cut(s) 181, 217, 223, 235, 256, 260, 280, 301, 344
HpyCH4IV ACGT 2 cut(s) 78, 146
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 46, 94, 127, 161, 164, 173, 182, 211
HpyF3I CTNAG 1 cut(s) 215
HpySE526I ACGT 2 cut(s) 78, 146
HspAI GCGC 5 cut(s) 40, 47, 74, 134, 386
KspI CCGCGG 1 cut(s) 156
LmnI GCTCC 4 cut(s) 62, 325, 395, 407
Lsp1109I GCAGC 1 cut(s) 66
MaeII ACGT 2 cut(s) 78, 146
MbiI CCGCTC 2 cut(s) 398, 410
MlyI GAGTC 1 cut(s) 99
MspA1I CMGCKG 2 cut(s) 130, 155
MspI CCGG 3 cut(s) 71, 117, 464
MspR9I CCNGG 2 cut(s) 71, 118
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 9 cut(s) 11, 46, 94, 127, 161, 164, 173, 182, 211
NciI CCSGG 2 cut(s) 71, 118
NlaIV GGNNCC 1 cut(s) 462
NmeAIII GCCGAG 1 cut(s) 123
NsbI TGCGCA 1 cut(s) 48
PfeI GAWTC 2 cut(s) 317, 454
PkrI GCNGC 7 cut(s) 7, 56, 96, 157, 166, 280, 364
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 2 cut(s) 150, 461
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SacII CCGCGG 1 cut(s) 156
SatI GCNGC 7 cut(s) 6, 55, 95, 156, 165, 279, 363
Sau96I GGNCC 2 cut(s) 150, 461
ScaI AGTACT 1 cut(s) 21
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 71, 118
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 69, 116
TaiI ACGT 2 cut(s) 81, 149
TatI WGTACW 1 cut(s) 19
TauI GCSGC 5 cut(s) 97, 158, 167, 281, 365
TfiI GAWTC 2 cut(s) 317, 454
TseI GCWGC 2 cut(s) 5, 54
VpaK11BI GGWCC 1 cut(s) 150
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.