Rw2G012680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014059
Physical Location & Seq
Forward (+)
13221857 .. 13222348
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012680.1

Sequence Viewer

Length: 492 bp
ATGGCTGCGAGAGCTCCGTCAGCTGTAGTAGGCCAAGTGCTCTCACTCCACCACCCCGTCCGCAATGCCCACCGTGCCTCCTCCCCCGCGCCGCCACATGGGGCCGCATTTCCCGGGCGGAGGGACCTGCTGCTGCTCTCGCTGACGTGGACGGCCTCGGCTGCTGTGGCGGCGCCCCCGGCAAAAGCGGATGACATCGGGCTGTTCGGGATACGAAAGAAGCTGAAGGAGGCGGAGAGAGAAACAGAAGTGATAGTGAAGGAAGGGTTCGAGGCGGCGGAGAAGGGCATAGAAACGGCGGAGAAGGGGTTAGAAACGGCTGGGAAGGGCATAGAAACGGCGGAGAAGGGGATCGAAACGGTGGAGAAGGGGATCGAAACGGTGGCGGGATTTGGCGGGTTGGCGCAGGCGGGACTCGTGGCGGGAGCCGAGGTTTTGGGGGTCGTGGTGGCCAGCTCCGTCGTCAATGCTATTTTGCGACCGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

16.69

Weight (kDa)

6.1

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23670
fragaria_vesca FvH4_1g14510
malus_domestica MD02G1158500.v1.1 MD15G1273300.v1.1
prunus_persica Prupe.7G145300_v2.0.a1
pyrus_communis pycom02g12500
rosa_chinensis RchiOBHm_Chr2g0103251
rosa_laevigata RLG00000017218
rosa_multiflora Rmu_sc0002218.1_g000001 Rmu_sc0006743.1_g000013
rosa_roxburghii Rroxscaffold_2G00139810
rosa_rugosa Rorug02G0112800
rosa_samantha Rh2AG161500 Rh2BG168300 Rh2CG168400 Rh2DG167200
rosa_wichuraiana Rw2G012680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 172
AccII CGCG 1 cut(s) 89
AclWI GGATC 2 cut(s) 359, 380
AcoI YGGCCR 1 cut(s) 450
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 98, 120
AjiI CACGTC 1 cut(s) 147
AluBI AGCT 4 cut(s) 14, 23, 223, 456
AluI AGCT 4 cut(s) 14, 23, 223, 456
Alw21I GWGCWC 2 cut(s) 16, 42
AlwI GGATC 2 cut(s) 359, 380
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 4 cut(s) 31, 102, 153, 450
ApeKI GCWGC 4 cut(s) 5, 130, 133, 161
AspLEI GCGC 3 cut(s) 91, 175, 406
AspS9I GGNCC 2 cut(s) 102, 124
AsuC2I CCSGG 3 cut(s) 114, 115, 179
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 452
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 16
BauI CACGAG 1 cut(s) 416
Bbv12I GWGCWC 2 cut(s) 16, 42
BbvI GCAGC 3 cut(s) 117, 120, 148
BceAI ACGGC 4 cut(s) 168, 312, 333, 354
BciVI GTATCC 1 cut(s) 204
BcnI CCSGG 3 cut(s) 114, 115, 179
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 135
BfuI GTATCC 1 cut(s) 204
BisI GCNGC 8 cut(s) 6, 92, 105, 131, 134, 162, 171, 276
BlsI GCNGC 8 cut(s) 7, 93, 106, 132, 135, 163, 172, 277
Bme1390I CCNGG 3 cut(s) 114, 115, 179
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 102, 124
BmiI GGNNCC 4 cut(s) 103, 125, 174, 427
BmrFI CCNGG 3 cut(s) 114, 115, 179
BpuMI CCSGG 3 cut(s) 114, 115, 179
BsaHI GRCGYC 1 cut(s) 173
BsaJI CCNNGG 4 cut(s) 113, 156, 177, 429
BsaWI WCCGGW 1 cut(s) 481
BsaXI ACNNNNNCTCC 6 cut(s) 62, 92, 293, 323, 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 120
Bse3DI GCAATG 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 113, 156, 177, 429
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 2 cut(s) 98, 120
BseMI GCAATG 1 cut(s) 70
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 3 cut(s) 117, 120, 148
BseYI CCCAGC 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 4 cut(s) 33, 104, 155, 452
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 2 cut(s) 16, 42
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 3 cut(s) 114, 179, 482
BslFI GGGAC 2 cut(s) 137, 426
BslI CCNNNNNNNGG 2 cut(s) 98, 120
BsmFI GGGAC 2 cut(s) 137, 426
BsnI GGCC 4 cut(s) 33, 104, 155, 452
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 16, 42
Bsp143I GATC 2 cut(s) 351, 372
BspANI GGCC 4 cut(s) 33, 104, 155, 452
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 4 cut(s) 103, 125, 174, 427
BspMI ACCTGC 1 cut(s) 135
BspPI GGATC 2 cut(s) 359, 380
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 70
BssECI CCNNGG 4 cut(s) 113, 156, 177, 429
BssMI GATC 2 cut(s) 351, 372
BssNI GRCGYC 1 cut(s) 173
BssSI CACGAG 1 cut(s) 416
Bst2BI CACGAG 1 cut(s) 416
Bst4CI ACNGT 3 cut(s) 74, 361, 382
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 408, 454
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 3 cut(s) 91, 175, 406
BstKTI GATC 2 cut(s) 354, 375
BstMBI GATC 2 cut(s) 351, 372
BstMCI CGRYCG 1 cut(s) 482
BstMWI GCNNNNNNNGC 8 cut(s) 11, 20, 74, 139, 161, 167, 170, 179
BstSCI CCNGG 3 cut(s) 112, 113, 177
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 3 cut(s) 117, 120, 148
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 4 cut(s) 33, 104, 155, 452
BtrI CACGTC 1 cut(s) 147
BtsCI GGATG 1 cut(s) 196
BveI ACCTGC 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 408, 454
CfoI GCGC 3 cut(s) 91, 175, 406
Cfr13I GGNCC 2 cut(s) 102, 124
Cfr9I CCCGGG 1 cut(s) 113
CviAII CATG 1 cut(s) 98
DinI GGCGCC 1 cut(s) 174
DpnI GATC 2 cut(s) 353, 374
DpnII GATC 2 cut(s) 351, 372
EaeI YGGCCR 1 cut(s) 450
EciI GGCGGA 5 cut(s) 133, 248, 293, 314, 356
Ecl136II GAGCTC 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 16
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
FaeI CATG 1 cut(s) 101
FaiI YATR 3 cut(s) 99, 290, 332
FaqI GGGAC 2 cut(s) 137, 426
FatI CATG 1 cut(s) 97
FauI CCCGC 5 cut(s) 94, 379, 389, 403, 415
Fnu4HI GCNGC 8 cut(s) 6, 92, 105, 131, 134, 162, 171, 276
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 8 cut(s) 6, 92, 105, 131, 134, 162, 171, 276
GlaI GCGC 3 cut(s) 90, 174, 405
GluI GCNGC 8 cut(s) 6, 92, 105, 131, 134, 162, 171, 276
GsaI CCCAGC 1 cut(s) 324
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 4 cut(s) 33, 104, 155, 452
HapII CCGG 3 cut(s) 114, 179, 482
HhaI GCGC 3 cut(s) 91, 175, 406
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 3 cut(s) 89, 173, 404
HinP1I GCGC 3 cut(s) 89, 173, 404
HinfI GANTC 1 cut(s) 414
HpaII CCGG 3 cut(s) 114, 179, 482
Hpy166II GTNNAC 1 cut(s) 150
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 8 cut(s) 220, 253, 257, 277, 298, 319, 340, 361
HpyCH4III ACNGT 3 cut(s) 74, 361, 382
HpyCH4IV ACGT 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 20, 74, 139, 161, 167, 170, 179
HpySE526I ACGT 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 3 cut(s) 89, 173, 404
KasI GGCGCC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 351, 372
LmnI GCTCC 3 cut(s) 19, 425, 461
LpnPI CCDG 6 cut(s) 127, 140, 192, 306, 392, 466
Lsp1109I GCAGC 3 cut(s) 117, 120, 148
MaeII ACGT 1 cut(s) 146
MalI GATC 2 cut(s) 353, 374
MboI GATC 2 cut(s) 351, 372
MhlI GDGCHC 2 cut(s) 16, 42
MlsI TGGCCA 1 cut(s) 452
MluNI TGGCCA 1 cut(s) 452
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 408
MnlI CCTC 7 cut(s) 88, 91, 114, 166, 223, 265, 424
Mox20I TGGCCA 1 cut(s) 452
MscI TGGCCA 1 cut(s) 452
Msp20I TGGCCA 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 114, 179, 482
MspR9I CCNGG 3 cut(s) 114, 115, 179
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 8 cut(s) 11, 20, 74, 139, 161, 167, 170, 179
NarI GGCGCC 1 cut(s) 173
NciI CCSGG 3 cut(s) 114, 115, 179
NdeII GATC 2 cut(s) 351, 372
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 4 cut(s) 103, 125, 174, 427
NmeAIII GCCGAG 2 cut(s) 137, 454
PkrI GCNGC 8 cut(s) 7, 93, 106, 132, 135, 163, 172, 277
PleI GAGTC 1 cut(s) 408
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 124
PspFI CCCAGC 1 cut(s) 320
PspN4I GGNNCC 4 cut(s) 103, 125, 174, 427
PspPI GGNCC 2 cut(s) 102, 124
PspPPI RGGWCCY 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 16
SatI GCNGC 8 cut(s) 6, 92, 105, 131, 134, 162, 171, 276
Sau3AI GATC 2 cut(s) 351, 372
Sau96I GGNCC 2 cut(s) 102, 124
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 3 cut(s) 114, 115, 179
SduI GDGCHC 2 cut(s) 16, 42
SetI ASST 7 cut(s) 16, 25, 129, 149, 225, 435, 458
SfcI CTRYAG 1 cut(s) 24
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 1 cut(s) 124
SmaI CCCGGG 1 cut(s) 115
SspDI GGCGCC 1 cut(s) 172
SstI GAGCTC 1 cut(s) 16
StyD4I CCNGG 3 cut(s) 112, 113, 177
TaaI ACNGT 3 cut(s) 74, 361, 382
TaiI ACGT 1 cut(s) 149
TaqI TCGA 3 cut(s) 270, 354, 375
TauI GCSGC 4 cut(s) 94, 107, 173, 278
TseI GCWGC 4 cut(s) 5, 130, 133, 161
TspGWI ACGGA 2 cut(s) 6, 448
TspMI CCCGGG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 124
XmaI CCCGGG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.