MD15G1273300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014059
Physical Location & Seq
Forward (+)
23847138 .. 23847632
495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1273300.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGCAGCAAGAGCATCAGTACTAGCTCTCCACCAAGTAAGCAATGCGCACCGCACCTCCACCTCCTCCCCGGCGCACGCCTCCAAGCCTGCGCCGCCTCGTGGACTCGCATTACCCGGGCGGAGACAGTTGGCGCTGTCGCTGACGTGGACGGCGGCGCTGGCAGCTGTGGCTGCGAAGGAAGTGAAGGCGGAGGACATTGGGCTGTTTGGGCTGAGGAGGAAGGTGAAAGAGGCGGAGAAGGAAGTGGAGGTGATAGTGAAGGAAGGGTTCGAGGCGGCGGAGAAGGGGCTGGAAACGACGGAAAAGGGGCTAGAATCGGCGGAGAAGGGGATAGAATCGGTGGAGCGAGGGATTGAAACGGCTGAGGAAGGGGTGGAAGCGGCGTTGAGCTTCGGGGGTTTGGCACAGGCTGGAGCGGTGGTGGGAGCGGAGGTTGTGGGGGTTTTGGTGGCGACTTCGATTGTGAATGGGATTTTGGGGCCGGATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

16.79

Weight (kDa)

5.49

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23670
fragaria_vesca FvH4_1g14510
malus_domestica MD02G1158500.v1.1 MD15G1273300.v1.1
prunus_persica Prupe.7G145300_v2.0.a1
pyrus_communis pycom02g12500
rosa_chinensis RchiOBHm_Chr2g0103251
rosa_laevigata RLG00000017218
rosa_multiflora Rmu_sc0002218.1_g000001 Rmu_sc0006743.1_g000013
rosa_roxburghii Rroxscaffold_2G00139810
rosa_rugosa Rorug02G0112800
rosa_samantha Rh2AG161500 Rh2BG168300 Rh2CG168400 Rh2DG167200
rosa_wichuraiana Rw2G012680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 48
AccBSI CCGCTC 2 cut(s) 419, 431
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 359
AjiI CACGTC 1 cut(s) 147
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 3 cut(s) 26, 167, 393
AluI AGCT 3 cut(s) 26, 167, 393
Alw26I GTCTC 1 cut(s) 118
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 482
ApeKI GCWGC 3 cut(s) 5, 164, 173
AspLEI GCGC 5 cut(s) 49, 76, 94, 136, 160
AspS9I GGNCC 1 cut(s) 482
AsuC2I CCSGG 3 cut(s) 71, 117, 118
AsuHPI GGTGA 2 cut(s) 238, 265
AvaI CYCGRG 1 cut(s) 116
BauI CACGAG 1 cut(s) 99
BbvCI CCTCAGC 2 cut(s) 215, 366
BbvI GCAGC 3 cut(s) 17, 160, 176
BceAI ACGGC 2 cut(s) 168, 378
BcnI CCSGG 3 cut(s) 71, 117, 118
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 2 cut(s) 23, 314
BfoI RGCGCY 2 cut(s) 137, 161
BisI GCNGC 7 cut(s) 6, 95, 156, 165, 174, 279, 384
BlsI GCNGC 7 cut(s) 7, 96, 157, 166, 175, 280, 385
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 3 cut(s) 71, 117, 118
BmeT110I CYCGRG 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 3 cut(s) 71, 117, 118
BmsI GCATC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 2 cut(s) 215, 366
BpuMI CCSGG 3 cut(s) 71, 117, 118
BsaJI CCNNGG 2 cut(s) 69, 116
BsaXI ACNNNNNCTCC 8 cut(s) 12, 41, 42, 71, 408, 420, 438, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 49
BseDI CCNNGG 2 cut(s) 69, 116
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 2 cut(s) 206, 357
BseRI GAGGAG 2 cut(s) 55, 232
BseXI GCAGC 3 cut(s) 17, 160, 176
BshFI GGCC 1 cut(s) 484
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 71, 117, 485
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 1 cut(s) 484
BsoBI CYCGRG 1 cut(s) 116
BspANI GGCC 1 cut(s) 484
BspCNI CTCAG 2 cut(s) 207, 358
BspLI GGNNCC 1 cut(s) 483
BsrBI CCGCTC 2 cut(s) 419, 431
BsrDI GCAATG 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 69, 116
BssSI CACGAG 1 cut(s) 99
Bst2BI CACGAG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 78, 90, 162
BstDEI CTNAG 2 cut(s) 215, 366
BstH2I RGCGCY 2 cut(s) 137, 161
BstHHI GCGC 5 cut(s) 49, 76, 94, 136, 160
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 7 cut(s) 11, 94, 161, 164, 170, 173, 211
BstSCI CCNGG 3 cut(s) 69, 115, 116
BstV1I GCAGC 3 cut(s) 17, 160, 176
BsuRI GGCC 1 cut(s) 484
BtrI CACGTC 1 cut(s) 147
Cac8I GCNNGC 3 cut(s) 78, 90, 162
CfoI GCGC 5 cut(s) 49, 76, 94, 136, 160
Cfr13I GGNCC 1 cut(s) 482
Cfr9I CCCGGG 1 cut(s) 116
Csp6I GTAC 1 cut(s) 20
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 2 cut(s) 215, 366
EciI GGCGGA 5 cut(s) 136, 206, 251, 296, 338
Eco88I CYCGRG 1 cut(s) 116
Fnu4HI GCNGC 7 cut(s) 6, 95, 156, 165, 174, 279, 384
Fsp4HI GCNGC 7 cut(s) 6, 95, 156, 165, 174, 279, 384
FspAI RTGCGCAY 1 cut(s) 48
FspBI CTAG 2 cut(s) 23, 314
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 5 cut(s) 48, 75, 93, 135, 159
GluI GCNGC 7 cut(s) 6, 95, 156, 165, 174, 279, 384
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 2 cut(s) 137, 161
HaeIII GGCC 1 cut(s) 484
HapII CCGG 3 cut(s) 71, 117, 485
HhaI GCGC 5 cut(s) 49, 76, 94, 136, 160
Hin6I GCGC 5 cut(s) 47, 74, 92, 134, 158
HinP1I GCGC 5 cut(s) 47, 74, 92, 134, 158
HinfI GANTC 3 cut(s) 105, 317, 338
HpaII CCGG 3 cut(s) 71, 117, 485
HphI GGTGA 2 cut(s) 238, 265
Hpy166II GTNNAC 2 cut(s) 104, 150
Hpy8I GTNNAC 2 cut(s) 104, 150
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 9 cut(s) 172, 181, 217, 235, 256, 260, 280, 322, 365
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 94, 161, 164, 170, 173, 211
HpyF3I CTNAG 2 cut(s) 215, 366
HpySE526I ACGT 1 cut(s) 146
HspAI GCGC 5 cut(s) 47, 74, 92, 134, 158
LmnI GCTCC 3 cut(s) 346, 416, 428
LpnPI CCDG 7 cut(s) 84, 102, 130, 146, 278, 395, 399
Lsp1109I GCAGC 3 cut(s) 17, 160, 176
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 23, 314
MaeII ACGT 1 cut(s) 146
MbiI CCGCTC 2 cut(s) 419, 431
MlyI GAGTC 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 3 cut(s) 71, 117, 485
MspR9I CCNGG 3 cut(s) 71, 117, 118
MwoI GCNNNNNNNGC 7 cut(s) 11, 94, 161, 164, 170, 173, 211
NciI CCSGG 3 cut(s) 71, 117, 118
NlaIV GGNNCC 1 cut(s) 483
NsbI TGCGCA 1 cut(s) 48
PfeI GAWTC 2 cut(s) 317, 338
PkrI GCNGC 7 cut(s) 7, 96, 157, 166, 175, 280, 385
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SatI GCNGC 7 cut(s) 6, 95, 156, 165, 174, 279, 384
Sau96I GGNCC 1 cut(s) 482
ScaI AGTACT 1 cut(s) 21
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 3 cut(s) 71, 117, 118
SetI ASST 9 cut(s) 28, 59, 65, 149, 169, 228, 255, 395, 438
SfaNI GCATC 1 cut(s) 23
SmaI CCCGGG 1 cut(s) 118
SspMI CTAG 2 cut(s) 23, 314
StyD4I CCNGG 3 cut(s) 69, 115, 116
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 149
TaqI TCGA 2 cut(s) 273, 461
TatI WGTACW 1 cut(s) 19
TauI GCSGC 4 cut(s) 97, 158, 281, 386
TfiI GAWTC 2 cut(s) 317, 338
TseI GCWGC 3 cut(s) 5, 164, 173
TspGWI ACGGA 1 cut(s) 317
TspMI CCCGGG 1 cut(s) 116
XmaI CCCGGG 1 cut(s) 116
XspI CTAG 2 cut(s) 23, 314
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.