RLG00000017218

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014059
Physical Location & Seq
Forward (+)
14222542 .. 14223012
471 bp
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UTR
Exon/CDS
Intron
RLM00000017218

Sequence Viewer

Length: 471 bp
ATGGCTGCGAGAGCTTCGTCAGTTGTAGTAGGCCAAGTGCTCTCACTCCACCACCCCGTCCGCAATGCCCACCGTGCCTCCTCCCCTGCGCCGCCAAGTGGGGCCTCATTTCCCGGCCGGAGGGAGCTGCTGCTGCTCTCGCTGACGTGGACGGCCTCGGCTGCTGTGGCGGCGGCCCCGGCAAAAGCGGAGGACATCGGGCTGTTCGGGATACGAAAGAAGCTGAAGGAGGCGGAGAGAGAAACAGAAGTGATAGTGAAGGAAGGGTTCGAGGCGGCGGAGAAGGGCATAGAAACGGCGGAGAAGGGTTTAGAAACCGCGGAGAAGGGCATAGAAACGGCGGAGAAGGGGATCGAAACGGTGGCGGGATTTGGCGGGTTGGCGCAGGCGGGACTGGTGGCGGGAGCCGAGGTTTTGGGGGTCGTGGTTGCCAGCTCCGTCGTCAATGCTATTCTGGGACCGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

15.89

Weight (kDa)

5.68

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23670
fragaria_vesca FvH4_1g14510
malus_domestica MD02G1158500.v1.1 MD15G1273300.v1.1
prunus_persica Prupe.7G145300_v2.0.a1
pyrus_communis pycom02g12500
rosa_chinensis RchiOBHm_Chr2g0103251
rosa_laevigata RLG00000017218
rosa_multiflora Rmu_sc0002218.1_g000001 Rmu_sc0006743.1_g000013
rosa_roxburghii Rroxscaffold_2G00139810
rosa_rugosa Rorug02G0112800
rosa_samantha Rh2AG161500 Rh2BG168300 Rh2CG168400 Rh2DG167200
rosa_wichuraiana Rw2G012680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 320
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 115
AcuI CTGAAG 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 98, 120
AjiI CACGTC 1 cut(s) 147
AluBI AGCT 4 cut(s) 14, 127, 223, 435
AluI AGCT 4 cut(s) 14, 127, 223, 435
Alw21I GWGCWC 1 cut(s) 42
AlwI GGATC 1 cut(s) 359
AoxI GGCC 5 cut(s) 31, 102, 115, 153, 174
ApeKI GCWGC 5 cut(s) 5, 127, 130, 133, 161
AspLEI GCGC 2 cut(s) 91, 385
AspS9I GGNCC 3 cut(s) 102, 175, 458
AsuC2I CCSGG 2 cut(s) 114, 179
AvaII GGWCC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 4 cut(s) 114, 117, 120, 148
BceAI ACGGC 3 cut(s) 168, 312, 354
BciVI GTATCC 1 cut(s) 204
BcnI CCSGG 2 cut(s) 114, 179
BfuI GTATCC 1 cut(s) 204
BisI GCNGC 9 cut(s) 6, 92, 128, 131, 134, 162, 171, 174, 276
BlsI GCNGC 9 cut(s) 7, 93, 129, 132, 135, 163, 172, 175, 277
Bme1390I CCNGG 2 cut(s) 114, 179
Bme18I GGWCC 1 cut(s) 458
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 3 cut(s) 102, 175, 458
BmiI GGNNCC 4 cut(s) 103, 177, 406, 459
BmrFI CCNGG 2 cut(s) 114, 179
BpuMI CCSGG 2 cut(s) 114, 179
BsaJI CCNNGG 4 cut(s) 156, 177, 318, 408
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 396, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 120
Bse1I ACTGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 156, 177, 318, 408
BseLI CCNNNNNNNGG 2 cut(s) 98, 120
BseMI GCAATG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 70
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 4 cut(s) 114, 117, 120, 148
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 5 cut(s) 33, 104, 117, 155, 176
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 4 cut(s) 114, 118, 179, 461
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 2 cut(s) 98, 120
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 5 cut(s) 33, 104, 117, 155, 176
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 1 cut(s) 351
BspANI GGCC 5 cut(s) 33, 104, 117, 155, 176
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 4 cut(s) 103, 177, 406, 459
BspPI GGATC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 70
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 4 cut(s) 156, 177, 318, 408
BssMI GATC 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 74, 361
BstC8I GCNNGC 2 cut(s) 387, 433
BstDSI CCRYGG 1 cut(s) 318
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 2 cut(s) 91, 385
BstKTI GATC 1 cut(s) 354
BstMBI GATC 1 cut(s) 351
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 8 cut(s) 11, 74, 133, 139, 161, 167, 170, 179
BstSCI CCNGG 2 cut(s) 112, 177
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 4 cut(s) 114, 117, 120, 148
BstZI CGGCCG 1 cut(s) 115
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 5 cut(s) 33, 104, 117, 155, 176
BtgI CCRYGG 1 cut(s) 318
BtrI CACGTC 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 387, 433
CfoI GCGC 2 cut(s) 91, 385
Cfr13I GGNCC 3 cut(s) 102, 175, 458
Cfr42I CCGCGG 1 cut(s) 321
DpnI GATC 1 cut(s) 353
DpnII GATC 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 115
EagI CGGCCG 1 cut(s) 115
EciI GGCGGA 4 cut(s) 248, 293, 314, 356
EclXI CGGCCG 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 458
Eco52I CGGCCG 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 102
FaiI YATR 2 cut(s) 290, 332
FaqI GGGAC 1 cut(s) 405
FauI CCCGC 4 cut(s) 358, 368, 382, 394
Fnu4HI GCNGC 9 cut(s) 6, 92, 128, 131, 134, 162, 171, 174, 276
Fsp4HI GCNGC 9 cut(s) 6, 92, 128, 131, 134, 162, 171, 174, 276
GlaI GCGC 2 cut(s) 90, 384
GluI GCNGC 9 cut(s) 6, 92, 128, 131, 134, 162, 171, 174, 276
HaeIII GGCC 5 cut(s) 33, 104, 117, 155, 176
HapII CCGG 4 cut(s) 114, 118, 179, 461
HhaI GCGC 2 cut(s) 91, 385
Hin6I GCGC 2 cut(s) 89, 383
HinP1I GCGC 2 cut(s) 89, 383
HpaII CCGG 4 cut(s) 114, 118, 179, 461
Hpy166II GTNNAC 1 cut(s) 150
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 7 cut(s) 220, 253, 257, 277, 298, 319, 340
HpyCH4III ACNGT 2 cut(s) 74, 361
HpyCH4IV ACGT 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 74, 133, 139, 161, 167, 170, 179
HpySE526I ACGT 1 cut(s) 146
HspAI GCGC 2 cut(s) 89, 383
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 1 cut(s) 351
LmnI GCTCC 3 cut(s) 124, 404, 440
LpnPI CCDG 8 cut(s) 99, 127, 131, 192, 371, 380, 440, 445
Lsp1109I GCAGC 4 cut(s) 114, 117, 120, 148
MaeII ACGT 1 cut(s) 146
MalI GATC 1 cut(s) 353
MboI GATC 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 42
MnlI CCTC 9 cut(s) 88, 91, 114, 115, 166, 184, 223, 265, 403
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 4 cut(s) 114, 118, 179, 461
MspR9I CCNGG 2 cut(s) 114, 179
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 8 cut(s) 11, 74, 133, 139, 161, 167, 170, 179
NciI CCSGG 2 cut(s) 114, 179
NdeII GATC 1 cut(s) 351
NlaIV GGNNCC 4 cut(s) 103, 177, 406, 459
NmeAIII GCCGAG 2 cut(s) 137, 433
PkrI GCNGC 9 cut(s) 7, 93, 129, 132, 135, 163, 172, 175, 277
PspN4I GGNNCC 4 cut(s) 103, 177, 406, 459
PspPI GGNCC 3 cut(s) 102, 175, 458
SacII CCGCGG 1 cut(s) 321
SatI GCNGC 9 cut(s) 6, 92, 128, 131, 134, 162, 171, 174, 276
Sau3AI GATC 1 cut(s) 351
Sau96I GGNCC 3 cut(s) 102, 175, 458
ScrFI CCNGG 2 cut(s) 114, 179
SduI GDGCHC 1 cut(s) 42
SetI ASST 6 cut(s) 16, 129, 149, 225, 414, 437
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 1 cut(s) 458
StyD4I CCNGG 2 cut(s) 112, 177
TaaI ACNGT 2 cut(s) 74, 361
TaiI ACGT 1 cut(s) 149
TaqI TCGA 2 cut(s) 270, 354
TauI GCSGC 4 cut(s) 94, 173, 176, 278
TseI GCWGC 5 cut(s) 5, 127, 130, 133, 161
TspGWI ACGGA 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.