Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0013554

Physical Location & Seq

Genomic Coordinates Reverse (-)

15873239 .. 15879783

Gene Structure 6545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1179100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGGAATCCCTCCTCCCTTATCCGTTCCCAATTAGAGTGACCACCAACCACCACCACTCCCTCCTACTGAGTCATCTCCCCACTACAACTCGACTCAACTCGCCCTCCCTTAAAACCATCTTCCACCACCCCCTCCTCCGCCCTCCCATCTTCCGCTTCAAATCCCTCCCTCCTCTTCCTCCTCCTCGTCTCCGCAATTCCAACACTCCCCGGTGCACCTCAGGTGCATGCGATCACGATCACGATCACGATCATGATCACCACCACCACCATAATCACGACCACCACCACCACCATCACCACCATCACCACGACTCTGCGGAGCTCACCGGATCGCAGAAACAGTTCGTCCGCTTCGCCAAGGCCGTGCGATGGACCGATCTCGCCGACTTCCTCAGGGAACACTTGCAATTGTGCTTCTGCTCCGCCGCTCTGCTTCTCGCCGCCGCCGCTTGCCCTTATTTGATGCCCAAGCCGGCCGTGAAGCCTATGCAGAACGCCTTCATACTCGTCGCTTTTCCTCTAGTGGGGGTCTCGGCGGCACTCGACGCCCTTACTGATTTAAGTGGCGGGAAGGTGAACATTCATGTATTGATGGCTTTAGCAGCATTTGCGTCTGTGTTTATGGGGAATGCATTAGAAGGAGGGTTACTTTTGGCCATGTTCAACCTTGCTCATATCGCTGAGGAGTATTTCACAAGTCGGTCTATGATTGACGTCAAAGAGTTGAAGGAAAATTATCCGGATTTTGCTCTTGTGCTGGATATCAATGACGGACAACTTCCTAATACATCCGAGTTGGAGTACAAGCAGGTTCCTGTGCACGACCTACAAGTGGGGTCGTACATTTTCGTCGGAGCTGGTGAGTCTGTGCCTGTAGATTGTGAAGTTTTCCAAGGTACTGCCACCATTACTATCGAGCACTTGACTGGAGAGATTAAACCGCTGGAGACAAAAGTTGGAGACAGGGTTCCTGGTGGTGCTAGGAACTTGGATGGCAGGATAATATTGAAGGCAACTAAAACTTGGAAAGAATCAACCCTTAGTAGAATTGTGCAATTGACTGAAGAAGCACAATTGAACAAACCAAAGCTTCAGAGGTGGTTGGATCAATTTGGAGAGCAATACAGCAAGGTGGTGGTAGTTTTGTCAGCTGCCATTGCTCTTCTTGGCCCATTCCTATTCAAGTGGCCATTTATTGGAACATCAGCTTGCAGAGGATCAGTTTATAGAGCATTGGGCCTCATGGTGGCAGCTTCACCATGTGCTTTGGCTGTAGCTCCATTGGCATATGCTACTGCAATTAGTTCCTGTGCAAGGAAGGGGATATTGCTGAAAGGTGGCCATGTGCTAGATGCTCTAGCTTCTTGTCACACTATTGCATTTGATAAAACTGGAACCTTGACAACCGGGGGGTTGGCGTTCAAAGCAATTGAACCAATTTACGGGCATCGGATGAGAAATAGTATATCAGATTTTTCTTCCTGTTGTTTCCCCAGCTGTGAAAAGCAGGCTCTTGCTGTTGCCGCTGCAATGGAGAAGGGTACCACTCACCCTATTGGAAGAGCTGTGGTCGACCATAGCGAAGGTAAAGACCTTCCTTCTGTTTCTATTGAGAGTTTTGAGTATTTTCCAGGCAGAGGTCTAATTGCTACTCTCAATGGCATCGAGTTAGGAACTGAAGGGGGTCAACTGTTGAAAGCATCTCTTGGTTCAGTAGATTTCATCACGTCACTTTGTAGATCAAAAGATGCATCAGAAAAGATCAAGGAAGCTGTTAGTGCATCTTCGTATGGAACAGAATTTGTTCGTGCTGCTCTTTCTGTCAATGAAAAGGTAACTTTGATTCACCTTGAGGATAGGCCTCGACCTGGGGTTTCGGATGTCATAGAAGAGTTAAAAGATCAAGCAAAGCTCCGTGTAATGATGTTGACTGGTGACCATGATTCAAGTGCTTGGAGAGTTGCAAATTCTGTTGGTATCAATGAAGTTTATTGCAGCCTGAAGCCTGAGGATAAGCTCAGTCATGTTAAGGATGTCTCAAGGGATACGGGTGGAGGTCTGATAATGGTCGGTGAAGGTATAAACGATGCTCCAGCGCTAGCAGCAGCCACTGTAGGGATTGTCCTTGCCCAACGTGCTAGTGCAACTGCCATAGCTGTTGCAGATGTTTTGCTGCTGCGAGACAATATATCTGCTGTACCATTCTGTATCGCCAAGTCTCGCCAAACAACTGCTTTGGTGAAGCAGAGTGTAGCCCTTGCTTTATCCTGTATAATTTTGGCTTCTCTTCCATCCGTATTAGGATTTCTTCCCCTCTGGTTAACGGTTCTATTACACGAAGGCGGTACCCTTCTTGTTTGCCTCAATTCCATCCGCGCTCTAAACCACCCAACGTGGTCCTGGAGGCAGGATTTATTGCATTTGGTGAACGAACTGAAATCTAAACTCATATCACCGAGGACACTTCAATCCAGCGGAAACACCATCCAAGCTGCAGCTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006873 GO:0006875 GO:0006878 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009642 GO:0009941 GO:0009987 GO:0015075 GO:0015318 GO:0015633 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0031967 GO:0031975 GO:0034220 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055069 GO:0055070 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072507 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase Hydrolase

Protein Analysis

836

Amino Acids

90.53

Weight (kDa)

7.01

Isoelectric Point (pI)

37.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	268 - 356	5.2e-23	P-type ATPase actuator domain
Hydrolase	PF00702	459 - 704	1e-20	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37270
fragaria_vesca	FvH4_1g16780 FvH4_1g16780 FvH4_1g16780
malus_domestica	MD02G1179100.v1.1 MD15G1288800.v1.1
prunus_persica	Prupe.6G228900_v2.0.a1
pyrus_communis	pycom02g14510
rosa_chinensis	RchiOBHm_Chr2g0106681
rosa_laevigata	RLG00000017530
rosa_multiflora	Rmu_sc0004000.1_g000005
rosa_roxburghii	Rroxscaffold_2G00136890
rosa_rugosa	Rorug02G0137700
rosa_samantha	Rh2AG188700 Rh2BG199900 Rh2CG193800 Rh2DG195000
rosa_wichuraiana	Rw2G014840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	720
Acc36I	ACCTGC	1 cut(s)	802
Acc65I	GGTACC	2 cut(s)	1544, 2348
AccB1I	GGYRCC	2 cut(s)	1544, 2348
AccB7I	CCANNNNNTGG	1 cut(s)	1199
AccBSI	CCGCTC	1 cut(s)	431
AccI	GTMKAC	1 cut(s)	1575
AccII	CGCG	1 cut(s)	2379
AccIII	TCCGGA	1 cut(s)	742
AclWI	GGATC	3 cut(s)	340, 1116, 1228
AcoI	YGGCCR	4 cut(s)	477, 657, 1190, 1342
AcsI	RAATTY	2 cut(s)	1802, 1969
AcuI	CTGAAG	4 cut(s)	1079, 1086, 1701, 2024
AcyI	GRCGYC	2 cut(s)	549, 717
AdeI	CACNNNGTG	1 cut(s)	1265
AfaI	GTAC	6 cut(s)	806, 845, 901, 1546, 2202, 2350
AfeI	AGCGCT	1 cut(s)	2100
AjiI	CACGTC	1 cut(s)	1731
AjnI	CCWGG	4 cut(s)	973, 1633, 1870, 2402
AloI	GAACNNNNNNTCC	2 cut(s)	954, 986
Alw21I	GWGCWC	4 cut(s)	218, 327, 825, 924
Alw26I	GTCTC	7 cut(s)	194, 538, 944, 957, 2044, 2178, 2226
Alw44I	GTGCAC	2 cut(s)	214, 821
AlwI	GGATC	3 cut(s)	340, 1116, 1228
AlwNI	CAGNNNCTG	1 cut(s)	2114
Aor13HI	TCCGGA	1 cut(s)	742
Aor51HI	AGCGCT	1 cut(s)	2100
AoxI	GGCC	8 cut(s)	363, 477, 657, 1171, 1190, 1240, 1342, 1862
ApaLI	GTGCAC	2 cut(s)	214, 821
ApoI	RAATTY	2 cut(s)	1802, 1969
Asp700I	GAANNNNTTC	3 cut(s)	344, 500, 1806
Asp718I	GGTACC	2 cut(s)	1544, 2348
AspLEI	GCGC	2 cut(s)	2101, 2381
AspS9I	GGNCC	4 cut(s)	375, 1172, 1240, 2400
AsuC2I	CCSGG	2 cut(s)	211, 1411
AsuNHI	GCTAGC	1 cut(s)	2101
AvaII	GGWCC	2 cut(s)	375, 2400
AxyI	CCTNAGG	3 cut(s)	220, 395, 2010
BaeGI	GKGCMC	2 cut(s)	218, 825
BalI	TGGCCA	3 cut(s)	659, 1192, 1344
BanI	GGYRCC	2 cut(s)	1544, 2348
BanII	GRGCYC	1 cut(s)	327
BarI	GAAGNNNNNNTAC	2 cut(s)	633, 665
Bbv12I	GWGCWC	4 cut(s)	218, 327, 825, 924
BbvCI	CCTCAGC	1 cut(s)	684
BceAI	ACGGC	2 cut(s)	350, 464
BciT130I	CCWGG	4 cut(s)	975, 1635, 1872, 2404
BciVI	GTATCC	1 cut(s)	2041
BclI	TGATCA	1 cut(s)	256
BcnI	CCSGG	2 cut(s)	211, 1411
BcoDI	GTCTC	7 cut(s)	194, 538, 944, 957, 2044, 2178, 2226
BfaI	CTAG	6 cut(s)	524, 984, 1352, 1361, 2102, 2142
BfmI	CTRYAG	4 cut(s)	876, 1275, 2115, 2496
BfoI	RGCGCY	1 cut(s)	2102
BfuAI	ACCTGC	1 cut(s)	802
BfuI	GTATCC	1 cut(s)	2041
Bme1390I	CCNGG	6 cut(s)	211, 975, 1411, 1635, 1872, 2404
Bme18I	GGWCC	2 cut(s)	375, 2400
BmgBI	CACGTC	1 cut(s)	1731
BmgT120I	GGNCC	4 cut(s)	375, 1172, 1240, 2400
BmiI	GGNNCC	5 cut(s)	816, 972, 1399, 1546, 2350
BmrFI	CCNGG	6 cut(s)	211, 975, 1411, 1635, 1872, 2404
BmsI	GCATC	9 cut(s)	456, 1345, 1459, 1674, 1712, 1741, 1763, 1793, 2080
BmtI	GCTAGC	1 cut(s)	2105
BpmI	CTGGAG	4 cut(s)	951, 968, 2079, 2425
Bpu10I	CCTNAGC	1 cut(s)	684
BpuEI	CTTGAG	2 cut(s)	1874, 2026
BpuMI	CCSGG	2 cut(s)	211, 1411
BsaBI	GATNNNNATC	4 cut(s)	237, 243, 249, 255
BsaHI	GRCGYC	2 cut(s)	549, 717
BsaI	GGTCTC	1 cut(s)	538
BsaJI	CCNNGG	6 cut(s)	209, 360, 895, 1410, 1871, 2459
BsaWI	WCCGGW	2 cut(s)	329, 742
BsaXI	ACNNNNNCTCC	6 cut(s)	41, 71, 89, 119, 954, 984
Bse118I	RCCGGY	1 cut(s)	475
Bse1I	ACTGG	3 cut(s)	934, 1399, 1939
Bse21I	CCTNAGG	3 cut(s)	220, 395, 2010
Bse3DI	GCAATG	2 cut(s)	1158, 1539
Bse8I	GATNNNNATC	4 cut(s)	237, 243, 249, 255
BseAI	TCCGGA	1 cut(s)	742
BseBI	CCWGG	4 cut(s)	975, 1635, 1872, 2404
BseDI	CCNNGG	6 cut(s)	209, 360, 895, 1410, 1871, 2459
BseGI	GGATG	8 cut(s)	791, 1000, 1461, 1888, 2041, 2294, 2373, 2487
BseJI	GATNNNNATC	4 cut(s)	237, 243, 249, 255
BseMI	GCAATG	2 cut(s)	1158, 1539
BseMII	CTCAG	7 cut(s)	59, 234, 409, 675, 2001, 2035, 2493
BseNI	ACTGG	3 cut(s)	934, 1399, 1939
BseRI	GAGGAG	5 cut(s)	125, 162, 171, 174, 701
BseSI	GKGCMC	2 cut(s)	218, 825
BseX3I	CGGCCG	1 cut(s)	477
BseYI	CCCAGC	1 cut(s)	1496
Bsh1236I	CGCG	1 cut(s)	2379
Bsh1285I	CGRYCG	1 cut(s)	480
BshFI	GGCC	8 cut(s)	365, 479, 659, 1173, 1192, 1242, 1344, 1864
BshNI	GGYRCC	2 cut(s)	1544, 2348
BsiEI	CGRYCG	1 cut(s)	480
BsiHKAI	GWGCWC	4 cut(s)	218, 327, 825, 924
BsiSI	CCGG	5 cut(s)	211, 330, 476, 743, 1410
BsmAI	GTCTC	7 cut(s)	194, 538, 944, 957, 2044, 2178, 2226
BsmBI	CGTCTC	1 cut(s)	194
BsmI	GAATGC	1 cut(s)	637
BsnI	GGCC	8 cut(s)	365, 479, 659, 1173, 1192, 1242, 1344, 1864
Bso31I	GGTCTC	1 cut(s)	538
Bsp1286I	GDGCHC	4 cut(s)	218, 327, 825, 924
Bsp13I	TCCGGA	1 cut(s)	742
BspANI	GGCC	8 cut(s)	365, 479, 659, 1173, 1192, 1242, 1344, 1864
BspCNI	CTCAG	7 cut(s)	60, 233, 408, 676, 2002, 2034, 2494
BspEI	TCCGGA	1 cut(s)	742
BspFNI	CGCG	1 cut(s)	2379
BspHI	TCATGA	1 cut(s)	253
BspLI	GGNNCC	5 cut(s)	816, 972, 1399, 1546, 2350
BspMAI	CTGCAG	1 cut(s)	2500
BspMI	ACCTGC	1 cut(s)	802
BspOI	GCTAGC	1 cut(s)	2105
BspPI	GGATC	3 cut(s)	340, 1116, 1228
BspQI	GCTCTTC	2 cut(s)	1170, 1558
BspT107I	GGYRCC	2 cut(s)	1544, 2348
BspTNI	GGTCTC	1 cut(s)	538
BsrBI	CCGCTC	1 cut(s)	431
BsrDI	GCAATG	2 cut(s)	1158, 1539
BsrFI	RCCGGY	1 cut(s)	475
BsrI	ACTGG	3 cut(s)	934, 1399, 1939
BssAI	RCCGGY	1 cut(s)	475
BssECI	CCNNGG	6 cut(s)	209, 360, 895, 1410, 1871, 2459
BssNI	GRCGYC	2 cut(s)	549, 717
BssT1I	CCWWGG	2 cut(s)	360, 895
Bst2UI	CCWGG	4 cut(s)	975, 1635, 1872, 2404
Bst4CI	ACNGT	4 cut(s)	345, 1695, 2116, 2329
Bst6I	CTCTTC	5 cut(s)	180, 1170, 1558, 1887, 2295
BstACI	GRCGYC	2 cut(s)	549, 717
BstAPI	GCANNNNNTGC	1 cut(s)	611
BstC8I	GCNNGC	6 cut(s)	229, 454, 477, 1213, 1512, 2103
BstDEI	CTNAG	8 cut(s)	68, 220, 395, 684, 1043, 2010, 2021, 2502
BstEII	GGTNACC	1 cut(s)	1937
BstENI	CCTNNNNNAGG	1 cut(s)	1315
BstF5I	GGATG	8 cut(s)	791, 1000, 1461, 1888, 2041, 2294, 2373, 2487
BstFNI	CGCG	1 cut(s)	2379
BstH2I	RGCGCY	1 cut(s)	2102
BstHHI	GCGC	2 cut(s)	2101, 2381
BstMAI	GTCTC	7 cut(s)	194, 538, 944, 957, 2044, 2178, 2226
BstMCI	CGRYCG	1 cut(s)	480
BstNI	CCWGG	4 cut(s)	975, 1635, 1872, 2404
BstNSI	RCATGY	1 cut(s)	231
BstPI	GGTNACC	1 cut(s)	1937
BstSCI	CCNGG	6 cut(s)	209, 973, 1409, 1633, 1870, 2402
BstSFI	CTRYAG	4 cut(s)	876, 1275, 2115, 2496
BstSLI	GKGCMC	2 cut(s)	218, 825
BstUI	CGCG	1 cut(s)	2379
BstZI	CGGCCG	1 cut(s)	477
Bsu36I	CCTNAGG	3 cut(s)	220, 395, 2010
BsuI	GTATCC	1 cut(s)	2041
BsuRI	GGCC	8 cut(s)	365, 479, 659, 1173, 1192, 1242, 1344, 1864
BtgZI	GCGATG	1 cut(s)	385
BtrI	CACGTC	1 cut(s)	1731
BtsCI	GGATG	8 cut(s)	791, 1000, 1461, 1888, 2041, 2294, 2373, 2487
BtsIMutI	CAGTG	1 cut(s)	2112
BveI	ACCTGC	1 cut(s)	802
Cac8I	GCNNGC	6 cut(s)	229, 454, 477, 1213, 1512, 2103
CaiI	CAGNNNCTG	1 cut(s)	2114
CciI	TCATGA	1 cut(s)	253
CfoI	GCGC	2 cut(s)	2101, 2381
Cfr10I	RCCGGY	1 cut(s)	475
Cfr13I	GGNCC	4 cut(s)	375, 1172, 1240, 2400
CseI	GACGC	2 cut(s)	557, 603
Csp6I	GTAC	6 cut(s)	805, 844, 900, 1545, 2201, 2349
CviAII	CATG	9 cut(s)	228, 254, 587, 661, 1246, 1263, 1346, 1943, 2027
CviQI	GTAC	6 cut(s)	805, 844, 900, 1545, 2201, 2349
DdeI	CTNAG	8 cut(s)	68, 220, 395, 684, 1043, 2010, 2021, 2502
DraIII	CACNNNGTG	1 cut(s)	1265
EaeI	YGGCCR	4 cut(s)	477, 657, 1190, 1342
EagI	CGGCCG	1 cut(s)	477
Eam1104I	CTCTTC	5 cut(s)	180, 1170, 1558, 1887, 2295
EarI	CTCTTC	5 cut(s)	180, 1170, 1558, 1887, 2295
EciI	GGCGGA	2 cut(s)	128, 415
Ecl136II	GAGCTC	1 cut(s)	325
EclXI	CGGCCG	1 cut(s)	477
Eco130I	CCWWGG	2 cut(s)	360, 895
Eco147I	AGGCCT	1 cut(s)	1864
Eco24I	GRGCYC	1 cut(s)	327
Eco31I	GGTCTC	1 cut(s)	538
Eco32I	GATATC	1 cut(s)	766
Eco47I	GGWCC	2 cut(s)	375, 2400
Eco47III	AGCGCT	1 cut(s)	2100
Eco52I	CGGCCG	1 cut(s)	477
Eco53kI	GAGCTC	1 cut(s)	325
Eco57I	CTGAAG	4 cut(s)	1079, 1086, 1701, 2024
Eco81I	CCTNAGG	3 cut(s)	220, 395, 2010
Eco91I	GGTNACC	1 cut(s)	1937
EcoICRI	GAGCTC	1 cut(s)	325
EcoNI	CCTNNNNNAGG	1 cut(s)	1315
EcoO65I	GGTNACC	1 cut(s)	1937
EcoRII	CCWGG	4 cut(s)	973, 1633, 1870, 2402
EcoRV	GATATC	1 cut(s)	766
EcoT14I	CCWWGG	2 cut(s)	360, 895
EcoT22I	ATGCAT	2 cut(s)	637, 1756
EcoT38I	GRGCYC	1 cut(s)	327
ErhI	CCWWGG	2 cut(s)	360, 895
Esp3I	CGTCTC	1 cut(s)	194
FaeI	CATG	9 cut(s)	231, 257, 590, 664, 1249, 1266, 1349, 1946, 2030
FalI	AAGNNNNNCTT	2 cut(s)	1692, 1724
FatI	CATG	9 cut(s)	227, 253, 586, 660, 1245, 1262, 1345, 1942, 2026
FauI	CCCGC	1 cut(s)	563
FauNDI	CATATG	1 cut(s)	1291
FbaI	TGATCA	1 cut(s)	256
FblI	GTMKAC	1 cut(s)	1575
FokI	GGATG	8 cut(s)	778, 1007, 1468, 1895, 2048, 2281, 2360, 2474
FriOI	GRGCYC	1 cut(s)	327
FspBI	CTAG	6 cut(s)	524, 984, 1352, 1361, 2102, 2142
GlaI	GCGC	2 cut(s)	2100, 2380
GsaI	CCCAGC	1 cut(s)	1500
GsuI	CTGGAG	4 cut(s)	951, 968, 2079, 2425
HaeII	RGCGCY	1 cut(s)	2102
HaeIII	GGCC	8 cut(s)	365, 479, 659, 1173, 1192, 1242, 1344, 1864
HapII	CCGG	5 cut(s)	211, 330, 476, 743, 1410
HgaI	GACGC	2 cut(s)	557, 603
HhaI	GCGC	2 cut(s)	2101, 2381
Hin1I	GRCGYC	2 cut(s)	549, 717
Hin1II	CATG	9 cut(s)	231, 257, 590, 664, 1249, 1266, 1349, 1946, 2030
Hin6I	GCGC	2 cut(s)	2099, 2379
HinP1I	GCGC	2 cut(s)	2099, 2379
HincII	GTYRAC	4 cut(s)	1576, 1691, 1932, 2325
HindII	GTYRAC	4 cut(s)	1576, 1691, 1932, 2325
HindIII	AAGCTT	1 cut(s)	1091
HinfI	GANTC	8 cut(s)	5, 70, 93, 314, 866, 1034, 1846, 1946
HpaI	GTTAAC	1 cut(s)	2325
HpaII	CCGG	5 cut(s)	211, 330, 476, 743, 1410
Hpy166II	GTNNAC	8 cut(s)	216, 580, 823, 1576, 1691, 1932, 2325, 2431
Hpy188I	TCNGA	8 cut(s)	796, 857, 1098, 1455, 1474, 1759, 1882, 2064
Hpy188III	TCNNGA	6 cut(s)	236, 242, 248, 254, 278, 743
Hpy8I	GTNNAC	8 cut(s)	216, 580, 823, 1576, 1691, 1932, 2325, 2431
Hpy99I	CGWCG	3 cut(s)	515, 551, 857
HpyCH4III	ACNGT	4 cut(s)	345, 1695, 2116, 2329
HpyCH4IV	ACGT	4 cut(s)	717, 1730, 2137, 2396
HpyF3I	CTNAG	8 cut(s)	68, 220, 395, 684, 1043, 2010, 2021, 2502
HpySE526I	ACGT	4 cut(s)	717, 1730, 2137, 2396
Hsp92I	GRCGYC	2 cut(s)	549, 717
Hsp92II	CATG	9 cut(s)	231, 257, 590, 664, 1249, 1266, 1349, 1946, 2030
HspAI	GCGC	2 cut(s)	2099, 2379
Kpn2I	TCCGGA	1 cut(s)	742
KpnI	GGTACC	2 cut(s)	1548, 2352
KroI	GCCGGC	1 cut(s)	475
KroNI	GCCGGC	1 cut(s)	477
Ksp22I	TGATCA	1 cut(s)	256
KspAI	GTTAAC	1 cut(s)	2325
LguI	GCTCTTC	2 cut(s)	1170, 1558
LmnI	GCTCC	6 cut(s)	322, 428, 857, 1285, 1920, 2098
LweI	GCATC	9 cut(s)	456, 1345, 1459, 1674, 1712, 1741, 1763, 1793, 2080
MaeI	CTAG	6 cut(s)	524, 984, 1352, 1361, 2102, 2142
MaeII	ACGT	4 cut(s)	717, 1730, 2137, 2396
MaeIII	GTNAC	6 cut(s)	37, 648, 1370, 1731, 1837, 1937
MbiI	CCGCTC	1 cut(s)	431
MfeI	CAATTG	4 cut(s)	410, 1058, 1076, 1431
MhlI	GDGCHC	4 cut(s)	218, 327, 825, 924
MlsI	TGGCCA	3 cut(s)	659, 1192, 1344
MluNI	TGGCCA	3 cut(s)	659, 1192, 1344
MlyI	GAGTC	4 cut(s)	79, 87, 308, 875
MmeI	TCCRAC	5 cut(s)	225, 780, 835, 940, 1086
Mox20I	TGGCCA	3 cut(s)	659, 1192, 1344
Mph1103I	ATGCAT	2 cut(s)	637, 1756
MroI	TCCGGA	1 cut(s)	742
MroNI	GCCGGC	1 cut(s)	475
MroXI	GAANNNNTTC	3 cut(s)	344, 500, 1806
MscI	TGGCCA	3 cut(s)	659, 1192, 1344
MseI	TTAA	6 cut(s)	111, 563, 939, 1898, 2031, 2324
MslI	CAYNNNNRTG	1 cut(s)	252
Msp20I	TGGCCA	3 cut(s)	659, 1192, 1344
MspA1I	CMGCKG	6 cut(s)	946, 1154, 1500, 1529, 2478, 2501
MspI	CCGG	5 cut(s)	211, 330, 476, 743, 1410
MspR9I	CCNGG	6 cut(s)	211, 975, 1411, 1635, 1872, 2404
MunI	CAATTG	4 cut(s)	410, 1058, 1076, 1431
Mva1269I	GAATGC	1 cut(s)	637
MvaI	CCWGG	4 cut(s)	975, 1635, 1872, 2404
MvnI	CGCG	1 cut(s)	2379
NaeI	GCCGGC	1 cut(s)	477
NciI	CCSGG	2 cut(s)	211, 1411
NdeI	CATATG	1 cut(s)	1291
NgoMIV	GCCGGC	1 cut(s)	475
NheI	GCTAGC	1 cut(s)	2101
NlaIII	CATG	9 cut(s)	231, 257, 590, 664, 1249, 1266, 1349, 1946, 2030
NlaIV	GGNNCC	5 cut(s)	816, 972, 1399, 1546, 2350
NmeAIII	GCCGAG	1 cut(s)	515
NmuCI	GTSAC	4 cut(s)	37, 1370, 1731, 1937
NsiI	ATGCAT	2 cut(s)	637, 1756
NspI	RCATGY	1 cut(s)	231
PaeI	GCATGC	1 cut(s)	231
PagI	TCATGA	1 cut(s)	253
PceI	AGGCCT	1 cut(s)	1864
PciSI	GCTCTTC	2 cut(s)	1170, 1558
PcsI	WCGNNNNNNNCGW	2 cut(s)	363, 1581
PctI	GAATGC	1 cut(s)	637
PdiI	GCCGGC	1 cut(s)	477
PdmI	GAANNNNTTC	3 cut(s)	344, 500, 1806
PfeI	GAWTC	4 cut(s)	5, 1034, 1846, 1946
PflMI	CCANNNNNTGG	1 cut(s)	1199
PfoI	TCCNGGA	1 cut(s)	2402
PleI	GAGTC	4 cut(s)	78, 87, 308, 874
PpsI	GAGTC	4 cut(s)	78, 87, 308, 874
Psp124BI	GAGCTC	1 cut(s)	327
Psp6I	CCWGG	4 cut(s)	973, 1633, 1870, 2402
PspEI	GGTNACC	1 cut(s)	1937
PspFI	CCCAGC	1 cut(s)	1496
PspGI	CCWGG	4 cut(s)	973, 1633, 1870, 2402
PspN4I	GGNNCC	5 cut(s)	816, 972, 1399, 1546, 2350
PspPI	GGNCC	4 cut(s)	375, 1172, 1240, 2400
PstI	CTGCAG	1 cut(s)	2500
PstNI	CAGNNNCTG	1 cut(s)	2114
PvuII	CAGCTG	3 cut(s)	1154, 1500, 2501
RsaI	GTAC	6 cut(s)	806, 845, 901, 1546, 2202, 2350
RsaNI	GTAC	6 cut(s)	805, 844, 900, 1545, 2201, 2349
RseI	CAYNNNNRTG	1 cut(s)	252
SacI	GAGCTC	1 cut(s)	327
SalI	GTCGAC	1 cut(s)	1574
SapI	GCTCTTC	2 cut(s)	1170, 1558
SaqAI	TTAA	6 cut(s)	111, 563, 939, 1898, 2031, 2324
Sau96I	GGNCC	4 cut(s)	375, 1172, 1240, 2400
SchI	GAGTC	4 cut(s)	79, 87, 308, 875
ScrFI	CCNGG	6 cut(s)	211, 975, 1411, 1635, 1872, 2404
SduI	GDGCHC	4 cut(s)	218, 327, 825, 924
SfaNI	GCATC	9 cut(s)	456, 1345, 1459, 1674, 1712, 1741, 1763, 1793, 2080
SfcI	CTRYAG	4 cut(s)	876, 1275, 2115, 2496
SinI	GGWCC	2 cut(s)	375, 2400
SmiMI	CAYNNNNRTG	1 cut(s)	252
SmlI	CTYRAG	2 cut(s)	1853, 2041
SmoI	CTYRAG	2 cut(s)	1853, 2041
SphI	GCATGC	1 cut(s)	231
SseBI	AGGCCT	1 cut(s)	1864
SspI	AATATT	1 cut(s)	1008
SspMI	CTAG	6 cut(s)	524, 984, 1352, 1361, 2102, 2142
SstI	GAGCTC	1 cut(s)	327
StuI	AGGCCT	1 cut(s)	1864
StyD4I	CCNGG	6 cut(s)	209, 973, 1409, 1633, 1870, 2402
StyI	CCWWGG	2 cut(s)	360, 895
TaaI	ACNGT	4 cut(s)	345, 1695, 2116, 2329
TaiI	ACGT	4 cut(s)	720, 1733, 2140, 2399
TaqI	TCGA	6 cut(s)	91, 546, 918, 1575, 1668, 1867
TaqII	GACCGA	2 cut(s)	392, 693
TatI	WGTACW	1 cut(s)	804
TauI	GCSGC	6 cut(s)	431, 446, 449, 452, 542, 1529
TfiI	GAWTC	4 cut(s)	5, 1034, 1846, 1946
Tru1I	TTAA	6 cut(s)	111, 563, 939, 1898, 2031, 2324
Tru9I	TTAA	6 cut(s)	111, 563, 939, 1898, 2031, 2324
TscAI	CASTG	1 cut(s)	2119
TseFI	GTSAC	4 cut(s)	37, 1370, 1731, 1937
Tsp45I	GTSAC	4 cut(s)	37, 1370, 1731, 1937
TspDTI	ATGAA	5 cut(s)	493, 575, 1714, 1845, 2001
TspGWI	ACGGA	4 cut(s)	12, 789, 1907, 2287
TspRI	CASTG	1 cut(s)	2119
Van91I	CCANNNNNTGG	1 cut(s)	1199
VneI	GTGCAC	2 cut(s)	214, 821
VpaK11BI	GGWCC	2 cut(s)	375, 2400
XagI	CCTNNNNNAGG	1 cut(s)	1315
XapI	RAATTY	2 cut(s)	1802, 1969
XceI	RCATGY	1 cut(s)	231
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2400
XmiI	GTMKAC	1 cut(s)	1575
XmnI	GAANNNNTTC	3 cut(s)	344, 500, 1806
XspI	CTAG	6 cut(s)	524, 984, 1352, 1361, 2102, 2142
ZraI	GACGTC	1 cut(s)	718
Zsp2I	ATGCAT	2 cut(s)	637, 1756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.