Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0013554

Physical Location & Seq

Genomic Coordinates Forward (+)

74557850 .. 74565501

Gene Structure 7652 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00136890.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGGAAACCCTAAAGCTTCCAATTTCAATCAGACTCCCCAATCACCACTCTCCTCTTCTCAACCACCACCACCACCCAACCACCACCGCCACTCGTCTCATCTCCCCAAAACCCATTCTCCGCCGCCTCCGCTCTCCTCTCTTTCGGTTCAAACCGTCTCTCCTTTCTCCCCCTCGAAATTTCCCTACTCTTCTTCGTTGCTCCGCCGCCGAGGTCCACTCCGATCACGACCACCACGAGCACCGCCACGACCACGATCACGATCACGATCACGGTCACCATCATCACCACCACGGCGGAGATTGTGCGGAGCTAACCGGGACTCAGAAAGCGTTCATCGGATTTGCAAAGGCTGTGCGATGGACCGACCTCGCCGATTTCCTCAGGGAGCACTTGCATTTGTGTTTCTGCTCCGCCGCGCTGTTCCTCGCCGCCGCCGCTTGCCCTTACGTGGTTCCCAAGCTCACCGTGAAGCCTCTGCAGAACGCTTTCATGATCGTCGCTTTTCCTCTAGTCGGAGTCTCTGCGGCACTTGATGCAATGACTGACATTAGTGGCGGGAAGGTGAACATTCATGTACTAATGGCTTTGGCGGCATTTGCCTCAGTGTTTATGGGGAATGCTCTTGAAGGAGGCTTGCTTTTGGCTATGTTCAATCTTGCTCATATCGCTGAGGAGTATTTTACCAGTCGCTCCATGATCGATGTGAAAGAGTTGAAAGAAAATTATCCGGATTCTTCCCTTGTTCTGGATATGGATGATGAACAGGTTCCTGATACATCTAATTTGAAGTACAAGCAGGTTCCTGTGCACGACCTACAAGTCGGGACATATATCTTGGTTGGAGCTGGAGAGTCTGTGCCTGTAGATTGTGAAGTGTTCCAAGGTAGTGCTACCATTACTATCGAGCACTTAACGGGAGAAGTTACGCCATTGGAGACAAAAGCTGGAGACATGATACCTGGTGGTGCTAGGAATTTGGATGGCAGGATAATAGTGAAGGCAAGAAAAAATTGGAAAGACTCAACCCTTAGCAGGATTGTGCAATTGACAGAAGAAGCACAATTAAATAAACCAAAGCTTCAGAGGTGGTTAGATCAGTTTGGCGAGCAATACAGCAAGGTGGTTGTAGTTTTGTCAGTCGCTGTTGCTCTACTTGGTCCATTCCTATTCAAGTGGCCGTTCATTGGTACATCAGCTTGCAGAGGATCAGTTTATAGAGCATTAGGCCTCATGGTGGCAGCATCACCGTGTGCTTTGGCTGTAGCTCCATTGGCCTATGCTACGGCAGTTAGCTCCTGCGCAAGGAAGGGTATATTGTTGAAAGGCGGTCATGTGCTAGATGCTCTAGCTTCCTGTCACACTATTGCATTTGATAAAACTGGGACCTTGACAACTGGGGGCCTAGCGTTTAAAGCGATTGAACCAATTTATGGACATCAAGTTAGAAACAATAAATCAGATTTTTCTTCCTGTTGTGTGCCAAGCTGTGAAAAGGAGGCCCTTGCTGTCGCTGCCGCAATGGAGAAGGGTACCACTCACCCTATTGGAAGAGCTGTTGTAGACCATAGTGAAGGGAAAGACCTTCCTTCTGTTTCTGTTGAGAGTTTTGAGTATTTTCCCGGCAGAGGTCTAGTTGCTACTGTCAATGGCATCGAGCTTCTTTTAAGTGTTCTTTTGGTAGATTTCATCACTTCACTGTGTTTGTCGGAAGATGCATCCAAAAAGATCAAGGAAGCTGTGAATGCTTCTTCTTACGGAACTGATTTTGTTCGTGCTGCTCTTTCAGTCGACGAAAAGGTAACACTGATTCACCTCGAGGATAGGCCTCGACCTGGGGTCTTGGATGTCATTGCCGAGTTACAAAATCAAGCAAAGCTCCGTATAATGATGTTAACTGGTGATCATGAGTCAAGTGCTTGGAGAGTTGCAAATGCTGTAGGTATTAATGAAGTTCATTGCAGCCTGAAGCCTGAGGATAAACTGAGTCATGTCAAGGGTGTCTCAAGGGATATGGGTATTAATGATGCTCCAGCACTTGCAGCTGCAACGGTAGGGATTGTCCTTGCTCAACGTGCTAGTGCAACTGCCACAGCTGTTGCTGATGTGTTGTTGCTGCGTGACAATATATCTGCCGTGCCATTCTGTATTGCCAAGTCTCGCCAAACAACTTCCCTGGTTAAGCAGAATGTAGTCCTTGCTTTAAGCTGTATAGCTTTGGCCTCTCTTCCATCTGTTTTAGGTTTTCTTCCCCTGTGGTTAACGGTTCTATTACATGAAGGCGGTACCCTTCTTGTCTGCCTCAACTCCATCCGTGCTCTAAACCACCCGACATGGAGCTGGAGGCAGGATTTATGGGATTTATTTAACCAACTGAAATCAAGACTTGAATTGTCAAGGAGACTTGCAACGAGCTCAAACCCCACCCAAGCTGCACCTCCGGTATCCTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006873 GO:0006875 GO:0006878 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009642 GO:0009941 GO:0009987 GO:0015075 GO:0015318 GO:0015633 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0031967 GO:0031975 GO:0034220 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055069 GO:0055070 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072507 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase Hydrolase

Protein Analysis

817

Amino Acids

88.62

Weight (kDa)

6.98

Isoelectric Point (pI)

35.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	260 - 352	3.7e-23	P-type ATPase actuator domain
Hydrolase	PF00702	455 - 682	5.8e-14	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37270
fragaria_vesca	FvH4_1g16780 FvH4_1g16780 FvH4_1g16780
malus_domestica	MD02G1179100.v1.1 MD15G1288800.v1.1
prunus_persica	Prupe.6G228900_v2.0.a1
pyrus_communis	pycom02g14510
rosa_chinensis	RchiOBHm_Chr2g0106681
rosa_laevigata	RLG00000017530
rosa_multiflora	Rmu_sc0004000.1_g000005
rosa_roxburghii	Rroxscaffold_2G00136890
rosa_rugosa	Rorug02G0137700
rosa_samantha	Rh2AG188700 Rh2BG199900 Rh2CG193800 Rh2DG195000
rosa_wichuraiana	Rw2G014840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2452
AasI	GACNNNNNNGTC	1 cut(s)	821
AbsI	CCTCGAGG	1 cut(s)	1817
Acc16I	TGCGCA	1 cut(s)	1303
Acc36I	ACCTGC	1 cut(s)	790
Acc65I	GGTACC	2 cut(s)	1532, 2285
AccB1I	GGYRCC	2 cut(s)	1532, 2285
AccB7I	CCANNNNNTGG	1 cut(s)	1433
AccBSI	CCGCTC	1 cut(s)	132
AccI	GTMKAC	2 cut(s)	1563, 1791
AccII	CGCG	1 cut(s)	419
AccIII	TCCGGA	1 cut(s)	730
AclWI	GGATC	1 cut(s)	1216
AcoI	YGGCCR	1 cut(s)	1178
AcsI	RAATTY	2 cut(s)	178, 976
AcuI	CTGAAG	2 cut(s)	1067, 1988
AdeI	CACNNNGTG	1 cut(s)	1253
AfaI	GTAC	5 cut(s)	579, 794, 1192, 1534, 2287
AhdI	GACNNNNNGTC	1 cut(s)	1838
AjnI	CCWGG	3 cut(s)	961, 1834, 2175
Alw21I	GWGCWC	6 cut(s)	243, 393, 813, 912, 2320, 2417
Alw26I	GTCTC	8 cut(s)	101, 162, 526, 932, 945, 2008, 2163, 2395
Alw44I	GTGCAC	1 cut(s)	809
AlwI	GGATC	1 cut(s)	1216
AlwNI	CAGNNNCTG	1 cut(s)	1145
Ama87I	CYCGRG	1 cut(s)	1817
Aor13HI	TCCGGA	1 cut(s)	730
AoxI	GGCC	7 cut(s)	1178, 1228, 1275, 1402, 1500, 1826, 2220
ApaLI	GTGCAC	1 cut(s)	809
ApeKI	GCWGC	8 cut(s)	1241, 1514, 1778, 1962, 2042, 2045, 2116, 2432
ApoI	RAATTY	2 cut(s)	178, 976
AseI	ATTAAT	2 cut(s)	1947, 2022
Asp700I	GAANNNNTTC	3 cut(s)	332, 488, 768
Asp718I	GGTACC	2 cut(s)	1532, 2285
AspLEI	GCGC	2 cut(s)	421, 1304
AspS9I	GGNCC	6 cut(s)	214, 363, 1160, 1386, 1402, 1501
AsuC2I	CCSGG	2 cut(s)	319, 1623
AsuHPI	GGTGA	9 cut(s)	35, 269, 278, 457, 577, 1239, 1532, 1805, 1913
AvaI	CYCGRG	1 cut(s)	1817
AvaII	GGWCC	4 cut(s)	214, 363, 1160, 1386
AxyI	CCTNAGG	2 cut(s)	383, 1974
BaeGI	GKGCMC	1 cut(s)	813
BanI	GGYRCC	2 cut(s)	1532, 2285
BanII	GRGCYC	1 cut(s)	2417
BauI	CACGAG	1 cut(s)	236
Bbv12I	GWGCWC	6 cut(s)	243, 393, 813, 912, 2320, 2417
BbvCI	CCTCAGC	1 cut(s)	672
BbvI	GCAGC	8 cut(s)	1253, 1501, 1765, 1974, 2032, 2054, 2103, 2419
BccI	CCATC	5 cut(s)	288, 354, 977, 2239, 2318
BceAI	ACGGC	4 cut(s)	310, 1165, 1302, 2120
BciT130I	CCWGG	3 cut(s)	963, 1836, 2177
BclI	TGATCA	1 cut(s)	1903
BcnI	CCSGG	2 cut(s)	319, 1623
BcoDI	GTCTC	8 cut(s)	101, 162, 526, 932, 945, 2008, 2163, 2395
BfaI	CTAG	7 cut(s)	512, 972, 1340, 1349, 1406, 1634, 2079
BfmI	CTRYAG	4 cut(s)	479, 864, 1263, 1938
BfuAI	ACCTGC	1 cut(s)	790
Bme1390I	CCNGG	5 cut(s)	319, 963, 1623, 1836, 2177
Bme18I	GGWCC	4 cut(s)	214, 363, 1160, 1386
BmeRI	GACNNNNNGTC	1 cut(s)	1838
BmeT110I	CYCGRG	1 cut(s)	1817
BmgT120I	GGNCC	6 cut(s)	214, 363, 1160, 1386, 1402, 1501
BmiI	GGNNCC	7 cut(s)	456, 771, 804, 1387, 1403, 1534, 2287
BmrFI	CCNGG	5 cut(s)	319, 963, 1623, 1836, 2177
BmrI	ACTGGG	2 cut(s)	1392, 1407
BmsI	GCATC	7 cut(s)	526, 1253, 1333, 1662, 1705, 1727, 2017
BmuI	ACTGGG	2 cut(s)	1392, 1407
BplI	GAGNNNNNCTC	2 cut(s)	203, 235
BpmI	CTGGAG	4 cut(s)	870, 969, 2016, 2362
Bpu10I	CCTNAGC	2 cut(s)	672, 1031
BpuEI	CTTGAG	1 cut(s)	1990
BpuMI	CCSGG	2 cut(s)	319, 1623
Bsa29I	ATCGAT	1 cut(s)	702
BsaAI	YACGTR	1 cut(s)	451
BsaBI	GATNNNNATC	2 cut(s)	261, 267
BsaJI	CCNNGG	5 cut(s)	210, 292, 883, 1835, 2175
BsaWI	WCCGGW	2 cut(s)	730, 2440
BsaXI	ACNNNNNCTCC	6 cut(s)	102, 132, 144, 174, 843, 873
Bse1I	ACTGG	4 cut(s)	687, 1387, 1402, 1903
Bse21I	CCTNAGG	2 cut(s)	383, 1974
Bse3DI	GCAATG	4 cut(s)	546, 1527, 1851, 1957
Bse8I	GATNNNNATC	2 cut(s)	261, 267
BseAI	TCCGGA	1 cut(s)	730
BseBI	CCWGG	3 cut(s)	963, 1836, 2177
BseCI	ATCGAT	1 cut(s)	702
BseDI	CCNNGG	5 cut(s)	210, 292, 883, 1835, 2175
BseGI	GGATG	5 cut(s)	763, 988, 1718, 1852, 2310
BseJI	GATNNNNATC	2 cut(s)	261, 267
BseMI	GCAATG	4 cut(s)	546, 1527, 1851, 1957
BseMII	CTCAG	6 cut(s)	338, 397, 618, 663, 1965, 1976
BseNI	ACTGG	4 cut(s)	687, 1387, 1402, 1903
BseRI	GAGGAG	3 cut(s)	42, 126, 689
BseSI	GKGCMC	1 cut(s)	813
BseXI	GCAGC	8 cut(s)	1253, 1501, 1765, 1974, 2032, 2054, 2103, 2419
BsgI	GTGCAG	1 cut(s)	2418
Bsh1236I	CGCG	1 cut(s)	419
BshFI	GGCC	7 cut(s)	1180, 1230, 1277, 1404, 1502, 1828, 2222
BshNI	GGYRCC	2 cut(s)	1532, 2285
BshVI	ATCGAT	1 cut(s)	702
BsiHKAI	GWGCWC	6 cut(s)	243, 393, 813, 912, 2320, 2417
BsiHKCI	CYCGRG	1 cut(s)	1817
BsiSI	CCGG	4 cut(s)	318, 731, 1623, 2441
BslFI	GGGAC	3 cut(s)	334, 841, 1399
BsmAI	GTCTC	8 cut(s)	101, 162, 526, 932, 945, 2008, 2163, 2395
BsmBI	CGTCTC	2 cut(s)	101, 162
BsmFI	GGGAC	3 cut(s)	334, 841, 1399
BsmI	GAATGC	2 cut(s)	625, 1750
BsnI	GGCC	7 cut(s)	1180, 1230, 1277, 1404, 1502, 1828, 2222
BsoBI	CYCGRG	1 cut(s)	1817
Bsp1286I	GDGCHC	6 cut(s)	243, 393, 813, 912, 2320, 2417
Bsp13I	TCCGGA	1 cut(s)	730
BspANI	GGCC	7 cut(s)	1180, 1230, 1277, 1404, 1502, 1828, 2222
BspCNI	CTCAG	6 cut(s)	337, 396, 617, 664, 1966, 1977
BspDI	ATCGAT	1 cut(s)	702
BspEI	TCCGGA	1 cut(s)	730
BspFNI	CGCG	1 cut(s)	419
BspHI	TCATGA	2 cut(s)	492, 1906
BspLI	GGNNCC	7 cut(s)	456, 771, 804, 1387, 1403, 1534, 2287
BspMAI	CTGCAG	1 cut(s)	483
BspMI	ACCTGC	1 cut(s)	790
BspPI	GGATC	1 cut(s)	1216
BspQI	GCTCTTC	1 cut(s)	1546
BspT107I	GGYRCC	2 cut(s)	1532, 2285
BsrBI	CCGCTC	1 cut(s)	132
BsrDI	GCAATG	4 cut(s)	546, 1527, 1851, 1957
BsrI	ACTGG	4 cut(s)	687, 1387, 1402, 1903
BssECI	CCNNGG	5 cut(s)	210, 292, 883, 1835, 2175
BssSI	CACGAG	1 cut(s)	236
BssT1I	CCWWGG	1 cut(s)	883
Bst2BI	CACGAG	1 cut(s)	236
Bst2UI	CCWGG	3 cut(s)	963, 1836, 2177
Bst4CI	ACNGT	8 cut(s)	156, 275, 469, 1251, 1645, 1701, 2053, 2266
Bst6I	CTCTTC	4 cut(s)	60, 195, 1546, 2232
BstAPI	GCANNNNNTGC	1 cut(s)	536
BstBAI	YACGTR	1 cut(s)	451
BstC8I	GCNNGC	4 cut(s)	442, 638, 1109, 1201
BstDEI	CTNAG	7 cut(s)	324, 383, 604, 672, 1031, 1974, 1985
BstDSI	CCRYGG	1 cut(s)	292
BstEII	GGTNACC	1 cut(s)	275
BstENI	CCTNNNNNAGG	1 cut(s)	1303
BstF5I	GGATG	5 cut(s)	763, 988, 1718, 1852, 2310
BstFNI	CGCG	1 cut(s)	419
BstHHI	GCGC	2 cut(s)	421, 1304
BstMAI	GTCTC	8 cut(s)	101, 162, 526, 932, 945, 2008, 2163, 2395
BstNI	CCWGG	3 cut(s)	963, 1836, 2177
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	5 cut(s)	317, 961, 1621, 1834, 2175
BstSFI	CTRYAG	4 cut(s)	479, 864, 1263, 1938
BstSLI	GKGCMC	1 cut(s)	813
BstUI	CGCG	1 cut(s)	419
BstV1I	GCAGC	8 cut(s)	1253, 1501, 1765, 1974, 2032, 2054, 2103, 2419
Bsu15I	ATCGAT	1 cut(s)	702
Bsu36I	CCTNAGG	2 cut(s)	383, 1974
BsuRI	GGCC	7 cut(s)	1180, 1230, 1277, 1404, 1502, 1828, 2222
BsuTUI	ATCGAT	1 cut(s)	702
BtgI	CCRYGG	1 cut(s)	292
BtgZI	GCGATG	1 cut(s)	373
BtsCI	GGATG	5 cut(s)	763, 988, 1718, 1852, 2310
BtsIMutI	CAGTG	3 cut(s)	612, 1697, 1805
BveI	ACCTGC	1 cut(s)	790
Cac8I	GCNNGC	4 cut(s)	442, 638, 1109, 1201
CaiI	CAGNNNCTG	1 cut(s)	1145
CciI	TCATGA	2 cut(s)	492, 1906
CfoI	GCGC	2 cut(s)	421, 1304
Cfr13I	GGNCC	6 cut(s)	214, 363, 1160, 1386, 1402, 1501
ClaI	ATCGAT	1 cut(s)	702
CsiI	ACCWGGT	1 cut(s)	961
Csp6I	GTAC	5 cut(s)	578, 793, 1191, 1533, 2286
CspCI	CAANNNNNGTGG	2 cut(s)	2080, 2115
CviQI	GTAC	5 cut(s)	578, 793, 1191, 1533, 2286
DdeI	CTNAG	7 cut(s)	324, 383, 604, 672, 1031, 1974, 1985
DraI	TTTAAA	1 cut(s)	1414
DraIII	CACNNNGTG	1 cut(s)	1253
DrdI	GACNNNNNNGTC	1 cut(s)	821
DriI	GACNNNNNGTC	1 cut(s)	1838
DseDI	GACNNNNNNGTC	1 cut(s)	821
EaeI	YGGCCR	1 cut(s)	1178
Eam1104I	CTCTTC	4 cut(s)	60, 195, 1546, 2232
Eam1105I	GACNNNNNGTC	1 cut(s)	1838
EarI	CTCTTC	4 cut(s)	60, 195, 1546, 2232
EciI	GGCGGA	4 cut(s)	110, 193, 312, 403
Ecl136II	GAGCTC	1 cut(s)	2415
Eco130I	CCWWGG	1 cut(s)	883
Eco147I	AGGCCT	2 cut(s)	1230, 1828
Eco24I	GRGCYC	1 cut(s)	2417
Eco47I	GGWCC	4 cut(s)	214, 363, 1160, 1386
Eco53kI	GAGCTC	1 cut(s)	2415
Eco57I	CTGAAG	2 cut(s)	1067, 1988
Eco81I	CCTNAGG	2 cut(s)	383, 1974
Eco88I	CYCGRG	1 cut(s)	1817
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	2415
EcoNI	CCTNNNNNAGG	1 cut(s)	1303
EcoO109I	RGGNCCY	3 cut(s)	1386, 1402, 1501
EcoO65I	GGTNACC	1 cut(s)	275
EcoRII	CCWGG	3 cut(s)	961, 1834, 2175
EcoT14I	CCWWGG	1 cut(s)	883
EcoT22I	ATGCAT	1 cut(s)	1720
EcoT38I	GRGCYC	1 cut(s)	2417
ErhI	CCWWGG	1 cut(s)	883
Esp3I	CGTCTC	2 cut(s)	101, 162
FaqI	GGGAC	3 cut(s)	334, 841, 1399
FauI	CCCGC	1 cut(s)	551
FbaI	TGATCA	1 cut(s)	1903
FblI	GTMKAC	2 cut(s)	1563, 1791
FokI	GGATG	5 cut(s)	770, 995, 1705, 1859, 2297
FriOI	GRGCYC	1 cut(s)	2417
FspBI	CTAG	7 cut(s)	512, 972, 1340, 1349, 1406, 1634, 2079
FspI	TGCGCA	1 cut(s)	1303
GlaI	GCGC	2 cut(s)	420, 1303
GsuI	CTGGAG	4 cut(s)	870, 969, 2016, 2362
HaeIII	GGCC	7 cut(s)	1180, 1230, 1277, 1404, 1502, 1828, 2222
HapII	CCGG	4 cut(s)	318, 731, 1623, 2441
HhaI	GCGC	2 cut(s)	421, 1304
Hin6I	GCGC	2 cut(s)	419, 1302
HinP1I	GCGC	2 cut(s)	419, 1302
HincII	GTYRAC	3 cut(s)	1792, 1896, 2262
HindII	GTYRAC	3 cut(s)	1792, 1896, 2262
HindIII	AAGCTT	2 cut(s)	14, 1079
HinfI	GANTC	9 cut(s)	33, 322, 519, 734, 854, 1022, 1810, 1910, 1987
HpaI	GTTAAC	2 cut(s)	1896, 2262
HpaII	CCGG	4 cut(s)	318, 731, 1623, 2441
HphI	GGTGA	9 cut(s)	35, 269, 278, 457, 577, 1239, 1532, 1805, 1913
Hpy166II	GTNNAC	7 cut(s)	217, 568, 811, 1564, 1792, 1896, 2262
Hpy188I	TCNGA	8 cut(s)	32, 223, 327, 341, 518, 1086, 1462, 1711
Hpy8I	GTNNAC	7 cut(s)	217, 568, 811, 1564, 1792, 1896, 2262
Hpy99I	CGWCG	2 cut(s)	503, 1796
HpyCH4III	ACNGT	8 cut(s)	156, 275, 469, 1251, 1645, 1701, 2053, 2266
HpyCH4IV	ACGT	2 cut(s)	450, 2074
HpyF3I	CTNAG	7 cut(s)	324, 383, 604, 672, 1031, 1974, 1985
HpySE526I	ACGT	2 cut(s)	450, 2074
HspAI	GCGC	2 cut(s)	419, 1302
Kpn2I	TCCGGA	1 cut(s)	730
KpnI	GGTACC	2 cut(s)	1536, 2289
Ksp22I	TGATCA	1 cut(s)	1903
KspAI	GTTAAC	2 cut(s)	1896, 2262
LguI	GCTCTTC	1 cut(s)	1546
Lsp1109I	GCAGC	8 cut(s)	1253, 1501, 1765, 1974, 2032, 2054, 2103, 2419
LweI	GCATC	7 cut(s)	526, 1253, 1333, 1662, 1705, 1727, 2017
MabI	ACCWGGT	1 cut(s)	961
MaeI	CTAG	7 cut(s)	512, 972, 1340, 1349, 1406, 1634, 2079
MaeII	ACGT	2 cut(s)	450, 2074
MaeIII	GTNAC	6 cut(s)	275, 925, 1358, 1801, 1860, 2120
MbiI	CCGCTC	1 cut(s)	132
MfeI	CAATTG	1 cut(s)	1046
MhlI	GDGCHC	6 cut(s)	243, 393, 813, 912, 2320, 2417
MlyI	GAGTC	7 cut(s)	27, 316, 528, 863, 1016, 1919, 1996
MmeI	TCCRAC	3 cut(s)	496, 823, 1689
Mph1103I	ATGCAT	1 cut(s)	1720
MroI	TCCGGA	1 cut(s)	730
MroXI	GAANNNNTTC	3 cut(s)	332, 488, 768
MslI	CAYNNNNRTG	1 cut(s)	1249
MspA1I	CMGCKG	2 cut(s)	2045, 2096
MspI	CCGG	4 cut(s)	318, 731, 1623, 2441
MspR9I	CCNGG	5 cut(s)	319, 963, 1623, 1836, 2177
MunI	CAATTG	1 cut(s)	1046
Mva1269I	GAATGC	2 cut(s)	625, 1750
MvaI	CCWGG	3 cut(s)	963, 1836, 2177
MvnI	CGCG	1 cut(s)	419
NciI	CCSGG	2 cut(s)	319, 1623
NlaIV	GGNNCC	7 cut(s)	456, 771, 804, 1387, 1403, 1534, 2287
NmeAIII	GCCGAG	2 cut(s)	235, 1882
NmuCI	GTSAC	3 cut(s)	275, 1358, 2120
NsbI	TGCGCA	1 cut(s)	1303
NsiI	ATGCAT	1 cut(s)	1720
PaeR7I	CTCGAG	1 cut(s)	1817
PagI	TCATGA	2 cut(s)	492, 1906
PceI	AGGCCT	2 cut(s)	1230, 1828
PciSI	GCTCTTC	1 cut(s)	1546
PcsI	WCGNNNNNNNCGW	1 cut(s)	234
PctI	GAATGC	2 cut(s)	625, 1750
PdmI	GAANNNNTTC	3 cut(s)	332, 488, 768
PfeI	GAWTC	2 cut(s)	734, 1810
PflMI	CCANNNNNTGG	1 cut(s)	1433
PleI	GAGTC	7 cut(s)	27, 316, 527, 862, 1016, 1918, 1995
PpsI	GAGTC	7 cut(s)	27, 316, 527, 862, 1016, 1918, 1995
Ppu21I	YACGTR	1 cut(s)	451
PpuMI	RGGWCCY	1 cut(s)	1386
PshBI	ATTAAT	2 cut(s)	1947, 2022
PsiI	TTATAA	1 cut(s)	2452
Psp124BI	GAGCTC	1 cut(s)	2417
Psp5II	RGGWCCY	1 cut(s)	1386
Psp6I	CCWGG	3 cut(s)	961, 1834, 2175
PspEI	GGTNACC	1 cut(s)	275
PspGI	CCWGG	3 cut(s)	961, 1834, 2175
PspN4I	GGNNCC	7 cut(s)	456, 771, 804, 1387, 1403, 1534, 2287
PspPI	GGNCC	6 cut(s)	214, 363, 1160, 1386, 1402, 1501
PspPPI	RGGWCCY	1 cut(s)	1386
PspXI	VCTCGAGB	1 cut(s)	1817
PstI	CTGCAG	1 cut(s)	483
PstNI	CAGNNNCTG	1 cut(s)	1145
PvuII	CAGCTG	2 cut(s)	2045, 2096
RsaI	GTAC	5 cut(s)	579, 794, 1192, 1534, 2287
RsaNI	GTAC	5 cut(s)	578, 793, 1191, 1533, 2286
RseI	CAYNNNNRTG	1 cut(s)	1249
SacI	GAGCTC	1 cut(s)	2417
SalI	GTCGAC	1 cut(s)	1790
SapI	GCTCTTC	1 cut(s)	1546
Sau96I	GGNCC	6 cut(s)	214, 363, 1160, 1386, 1402, 1501
SchI	GAGTC	7 cut(s)	27, 316, 528, 863, 1016, 1919, 1996
ScrFI	CCNGG	5 cut(s)	319, 963, 1623, 1836, 2177
SduI	GDGCHC	6 cut(s)	243, 393, 813, 912, 2320, 2417
SexAI	ACCWGGT	1 cut(s)	961
SfaNI	GCATC	7 cut(s)	526, 1253, 1333, 1662, 1705, 1727, 2017
SfcI	CTRYAG	4 cut(s)	479, 864, 1263, 1938
Sfr274I	CTCGAG	1 cut(s)	1817
SinI	GGWCC	4 cut(s)	214, 363, 1160, 1386
SlaI	CTCGAG	1 cut(s)	1817
SmiMI	CAYNNNNRTG	1 cut(s)	1249
SmlI	CTYRAG	2 cut(s)	1817, 2005
SmoI	CTYRAG	2 cut(s)	1817, 2005
SseBI	AGGCCT	2 cut(s)	1230, 1828
SspMI	CTAG	7 cut(s)	512, 972, 1340, 1349, 1406, 1634, 2079
SstI	GAGCTC	1 cut(s)	2417
StuI	AGGCCT	2 cut(s)	1230, 1828
StyD4I	CCNGG	5 cut(s)	317, 961, 1621, 1834, 2175
StyI	CCWWGG	1 cut(s)	883
TaaI	ACNGT	8 cut(s)	156, 275, 469, 1251, 1645, 1701, 2053, 2266
TaiI	ACGT	2 cut(s)	453, 2077
TaqI	TCGA	7 cut(s)	175, 702, 906, 1656, 1791, 1818, 1831
TaqII	GACCGA	1 cut(s)	380
TatI	WGTACW	2 cut(s)	577, 792
TauI	GCSGC	9 cut(s)	126, 209, 419, 434, 437, 440, 530, 596, 1520
TfiI	GAWTC	2 cut(s)	734, 1810
TscAI	CASTG	3 cut(s)	612, 1704, 1812
TseFI	GTSAC	3 cut(s)	275, 1358, 2120
TseI	GCWGC	8 cut(s)	1241, 1514, 1778, 1962, 2042, 2045, 2116, 2432
Tsp45I	GTSAC	3 cut(s)	275, 1358, 2120
TspDTI	ATGAA	9 cut(s)	325, 481, 563, 777, 1174, 1678, 1946, 1965, 2292
TspGWI	ACGGA	3 cut(s)	1773, 1871, 2303
TspRI	CASTG	3 cut(s)	612, 1704, 1812
Van91I	CCANNNNNTGG	1 cut(s)	1433
VneI	GTGCAC	1 cut(s)	809
VpaK11BI	GGWCC	4 cut(s)	214, 363, 1160, 1386
VspI	ATTAAT	2 cut(s)	1947, 2022
XagI	CCTNNNNNAGG	1 cut(s)	1303
XapI	RAATTY	2 cut(s)	178, 976
XhoI	CTCGAG	1 cut(s)	1817
XmiI	GTMKAC	2 cut(s)	1563, 1791
XmnI	GAANNNNTTC	3 cut(s)	332, 488, 768
XspI	CTAG	7 cut(s)	512, 972, 1340, 1349, 1406, 1634, 2079
Zsp2I	ATGCAT	1 cut(s)	1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.