MD02G1181300.v1.1

Sigma factor sigB regulation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013318
Physical Location & Seq
Reverse (-)
16296788 .. 16298402
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1181300.v1.1.491

Sequence Viewer

Length: 894 bp
ATGTGGTGCCAGTTGGGACCTGTCAGCCAAACTAACCAACAATCAAGCAACAGAAAGCGGAAGAAGAGACAGAGAGCCGCAATGGGAATAGTGGAAGAAGCTCACAACGTGCGCGTCTTAGGGTCGGGTCAGCAAGTCCTAGTCCTGGCCCACGGCTTCGGCACCGATCAGTCGGTCTGGAAGCACCTCGTCCCCCACCTCGTCGACGACTACCGCGTCATTATGTACGACAACATGGGCGCTGGAACCACCAACCCTGAATACTTCGACTTCGAGCGCTACTCCACCCTCGAAGGCTACGCCTACGACCTCCTCGCCATTCTCGAGGAGCTCCGCGTCGGGTCATGCATATTCGTCGGCCACTCCGTCTCAGGCATGATCGGAGCCATAGCCGCCATCACCCGACCGGACCTCTTCACCAAGCTCGTCATGATCGCCGCCTCCCCTAGGTATCTCAACGACGTGGACTACTACGGAGGATTCGAGCAAGAAGAGTTGGAGCAACTATTCGAGGCGATCCGGTCCAACTACAAGGCATGGTGCTCCGGATTTGCGCCGCTGGCGGTGGGCGGCGACATGGACTCGGTGGCGGTCCAGGAGTTCAGCCGGACCGTATTCAACATGCGGCCGGACATTGCCCTTAGCGTGGCACAAACCATATTCCAAAGCGACCTGAGACAAATGCTTGGGTTGATCACGGTGCCATGTCACATATTACAGAGTGTAAAAGACCTTGCTGTCCCTGTGGTTGTGTCCGAGTATTTGCACCAGAATCTTGGCGGCCAGTCGATCGTCGAGGTCATGTCGTCAGACGGTCATCTTCCGCAACTGAGCTCGCCGGAGATTGTGAATCCGGTGCTGCTCAGGCACATCAGATATGACATAGCCGTGTAA

Protein Analysis

298

Amino Acids

33.07

Weight (kDa)

5.45

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 44 - 261 1.3e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 46 - 279 1.1e-18 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 47 - 284 2.3e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37470
fragaria_vesca FvH4_1g17110
malus_domestica MD02G1181300.v1.1 MD02G1181500.v1.1
prunus_persica Prupe.6G225000_v2.0.a1
pyrus_communis pycom02g14720 pycom02g14730
rosa_chinensis RchiOBHm_Chr2g0107281
rosa_laevigata RLG00000017567
rosa_multiflora Rmu_co8389809.1_g000001 Rmu_sc0006416.1_g000017
rosa_roxburghii Rroxscaffold_2G00136470
rosa_rugosa Rorug02G0141400
rosa_samantha Rh2AG192400 Rh2BG204300 Rh2CG197600 Rh2DG199600
rosa_wichuraiana Rw2G015220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 215, 737
AccB1I GGYRCC 3 cut(s) 6, 161, 700
AccI GTMKAC 1 cut(s) 204
AccII CGCG 3 cut(s) 114, 216, 336
AccIII TCCGGA 1 cut(s) 545
AclWI GGATC 1 cut(s) 511
AcoI YGGCCR 3 cut(s) 358, 626, 781
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 1 cut(s) 227
AfeI AGCGCT 1 cut(s) 278
AfiI CCNNNNNNNGG 3 cut(s) 145, 447, 646
AgsI TTSAA 1 cut(s) 619
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 144, 594
AluBI AGCT 4 cut(s) 101, 331, 424, 834
AluI AGCT 4 cut(s) 101, 331, 424, 834
Alw21I GWGCWC 3 cut(s) 333, 545, 836
Alw26I GTCTC 3 cut(s) 61, 373, 670
AlwI GGATC 1 cut(s) 511
Ama87I CYCGRG 1 cut(s) 323
Aor13HI TCCGGA 1 cut(s) 545
Aor51HI AGCGCT 1 cut(s) 278
AoxI GGCC 4 cut(s) 147, 358, 626, 781
ApeKI GCWGC 1 cut(s) 859
AspA2I CCTAGG 1 cut(s) 446
AspLEI GCGC 4 cut(s) 114, 242, 279, 556
AspS9I GGNCC 6 cut(s) 17, 148, 409, 522, 592, 609
AsuHPI GGTGA 2 cut(s) 391, 409
AvaI CYCGRG 1 cut(s) 323
AvaII GGWCC 5 cut(s) 17, 409, 522, 592, 609
AvrII CCTAGG 1 cut(s) 446
BanI GGYRCC 3 cut(s) 6, 161, 700
BanII GRGCYC 2 cut(s) 333, 836
Bbv12I GWGCWC 3 cut(s) 333, 545, 836
BbvI GCAGC 1 cut(s) 846
BccI CCATC 1 cut(s) 404
BceAI ACGGC 2 cut(s) 169, 872
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 2 cut(s) 146, 596
BclI TGATCA 1 cut(s) 693
BcoDI GTCTC 3 cut(s) 61, 373, 670
BfaI CTAG 2 cut(s) 140, 447
BfoI RGCGCY 2 cut(s) 243, 280
BisI GCNGC 8 cut(s) 78, 393, 438, 557, 571, 626, 781, 860
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 8 cut(s) 79, 394, 439, 558, 572, 627, 782, 861
Bme1390I CCNGG 2 cut(s) 146, 596
Bme18I GGWCC 5 cut(s) 17, 409, 522, 592, 609
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 6 cut(s) 17, 148, 409, 522, 592, 609
BmiI GGNNCC 6 cut(s) 8, 18, 163, 247, 385, 702
BmrFI CCNGG 2 cut(s) 146, 596
BplI GAGNNNNNCTC 2 cut(s) 266, 298
Bpu10I CCTNAGC 2 cut(s) 641, 863
BsaJI CCNNGG 2 cut(s) 151, 446
BsaWI WCCGGW 4 cut(s) 406, 519, 545, 853
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 447, 646
Bse1I ACTGG 2 cut(s) 10, 784
Bse3DI GCAATG 2 cut(s) 87, 633
BseAI TCCGGA 1 cut(s) 545
BseBI CCWGG 2 cut(s) 146, 596
BseDI CCNNGG 2 cut(s) 151, 446
BseLI CCNNNNNNNGG 3 cut(s) 145, 447, 646
BseMI GCAATG 2 cut(s) 87, 633
BseMII CTCAG 4 cut(s) 384, 665, 821, 877
BseNI ACTGG 2 cut(s) 10, 784
BseRI GAGGAG 2 cut(s) 302, 341
BseX3I CGGCCG 1 cut(s) 626
BseXI GCAGC 1 cut(s) 846
Bsh1236I CGCG 3 cut(s) 114, 216, 336
Bsh1285I CGRYCG 3 cut(s) 407, 629, 792
BshFI GGCC 4 cut(s) 149, 360, 628, 783
BshNI GGYRCC 3 cut(s) 6, 161, 700
BsiEI CGRYCG 3 cut(s) 407, 629, 792
BsiHKAI GWGCWC 3 cut(s) 333, 545, 836
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 7 cut(s) 407, 520, 546, 607, 629, 839, 854
BslFI GGGAC 3 cut(s) 30, 176, 725
BslI CCNNNNNNNGG 3 cut(s) 145, 447, 646
BsmAI GTCTC 3 cut(s) 61, 373, 670
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 3 cut(s) 30, 176, 725
BsnI GGCC 4 cut(s) 149, 360, 628, 783
BsoBI CYCGRG 1 cut(s) 323
Bsp1286I GDGCHC 3 cut(s) 333, 545, 836
Bsp13I TCCGGA 1 cut(s) 545
Bsp143I GATC 6 cut(s) 166, 378, 432, 516, 693, 789
BspANI GGCC 4 cut(s) 149, 360, 628, 783
BspCNI CTCAG 4 cut(s) 383, 666, 822, 876
BspEI TCCGGA 1 cut(s) 545
BspFNI CGCG 3 cut(s) 114, 216, 336
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 6 cut(s) 8, 18, 163, 247, 385, 702
BspPI GGATC 1 cut(s) 511
BspT107I GGYRCC 3 cut(s) 6, 161, 700
BsrDI GCAATG 2 cut(s) 87, 633
BsrI ACTGG 2 cut(s) 10, 784
BssECI CCNNGG 2 cut(s) 151, 446
BssMI GATC 6 cut(s) 166, 378, 432, 516, 693, 789
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 2 cut(s) 146, 596
Bst4CI ACNGT 3 cut(s) 613, 700, 815
Bst6I CTCTTC 3 cut(s) 59, 419, 486
BstC8I GCNNGC 2 cut(s) 561, 836
BstDEI CTNAG 6 cut(s) 118, 370, 641, 674, 830, 863
BstDSI CCRYGG 1 cut(s) 151
BstENI CCTNNNNNAGG 1 cut(s) 445
BstFNI CGCG 3 cut(s) 114, 216, 336
BstH2I RGCGCY 2 cut(s) 243, 280
BstHHI GCGC 4 cut(s) 114, 242, 279, 556
BstKTI GATC 6 cut(s) 169, 381, 435, 519, 696, 792
BstMAI GTCTC 3 cut(s) 61, 373, 670
BstMBI GATC 6 cut(s) 166, 378, 432, 516, 693, 789
BstMCI CGRYCG 3 cut(s) 407, 629, 792
BstMWI GCNNNNNNNGC 3 cut(s) 392, 560, 865
BstNI CCWGG 2 cut(s) 146, 596
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 2 cut(s) 144, 594
BstUI CGCG 3 cut(s) 114, 216, 336
BstV1I GCAGC 1 cut(s) 846
BstXI CCANNNNNNTGG 1 cut(s) 776
BstZI CGGCCG 1 cut(s) 626
BsuRI GGCC 4 cut(s) 149, 360, 628, 783
BtgI CCRYGG 1 cut(s) 151
BtrI CACGTC 1 cut(s) 463
Cac8I GCNNGC 2 cut(s) 561, 836
CciI TCATGA 1 cut(s) 429
CfoI GCGC 4 cut(s) 114, 242, 279, 556
Cfr13I GGNCC 6 cut(s) 17, 148, 409, 522, 592, 609
CpoI CGGWCCG 1 cut(s) 609
CseI GACGC 3 cut(s) 103, 205, 325
Csp6I GTAC 1 cut(s) 226
CspI CGGWCCG 1 cut(s) 609
CviAII CATG 9 cut(s) 235, 345, 376, 430, 537, 577, 622, 705, 802
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 6 cut(s) 118, 370, 641, 674, 830, 863
DpnI GATC 6 cut(s) 168, 380, 434, 518, 695, 791
DpnII GATC 6 cut(s) 166, 378, 432, 516, 693, 789
DraIII CACNNNGTG 1 cut(s) 109
DrdI GACNNNNNNGTC 2 cut(s) 215, 737
DseDI GACNNNNNNGTC 2 cut(s) 215, 737
EaeI YGGCCR 3 cut(s) 358, 626, 781
EagI CGGCCG 1 cut(s) 626
Eam1104I CTCTTC 3 cut(s) 59, 419, 486
EarI CTCTTC 3 cut(s) 59, 419, 486
Ecl136II GAGCTC 2 cut(s) 331, 834
EclXI CGGCCG 1 cut(s) 626
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 2 cut(s) 333, 836
Eco47I GGWCC 5 cut(s) 17, 409, 522, 592, 609
Eco47III AGCGCT 1 cut(s) 278
Eco52I CGGCCG 1 cut(s) 626
Eco53kI GAGCTC 2 cut(s) 331, 834
Eco88I CYCGRG 1 cut(s) 323
EcoICRI GAGCTC 2 cut(s) 331, 834
EcoNI CCTNNNNNAGG 1 cut(s) 445
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 144, 594
EcoT14I CCWWGG 1 cut(s) 446
EcoT22I ATGCAT 1 cut(s) 350
EcoT38I GRGCYC 2 cut(s) 333, 836
ErhI CCWWGG 1 cut(s) 446
Esp3I CGTCTC 1 cut(s) 373
FaeI CATG 9 cut(s) 238, 348, 379, 433, 540, 580, 625, 708, 805
FaqI GGGAC 3 cut(s) 30, 176, 725
FatI CATG 9 cut(s) 234, 344, 375, 429, 536, 576, 621, 704, 801
FbaI TGATCA 1 cut(s) 693
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 8 cut(s) 78, 393, 438, 557, 571, 626, 781, 860
FriOI GRGCYC 2 cut(s) 333, 836
Fsp4HI GCNGC 8 cut(s) 78, 393, 438, 557, 571, 626, 781, 860
FspBI CTAG 2 cut(s) 140, 447
GlaI GCGC 4 cut(s) 113, 241, 278, 555
GluI GCNGC 8 cut(s) 78, 393, 438, 557, 571, 626, 781, 860
HaeII RGCGCY 2 cut(s) 243, 280
HaeIII GGCC 4 cut(s) 149, 360, 628, 783
HapII CCGG 7 cut(s) 407, 520, 546, 607, 629, 839, 854
HgaI GACGC 3 cut(s) 103, 205, 325
HhaI GCGC 4 cut(s) 114, 242, 279, 556
Hin1II CATG 9 cut(s) 238, 348, 379, 433, 540, 580, 625, 708, 805
Hin6I GCGC 4 cut(s) 112, 240, 277, 554
HinP1I GCGC 4 cut(s) 112, 240, 277, 554
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 4 cut(s) 480, 581, 772, 850
HpaII CCGG 7 cut(s) 407, 520, 546, 607, 629, 839, 854
HphI GGTGA 2 cut(s) 391, 409
Hpy166II GTNNAC 2 cut(s) 205, 466
Hpy188I TCNGA 4 cut(s) 383, 757, 811, 875
Hpy188III TCNNGA 4 cut(s) 178, 323, 430, 546
Hpy8I GTNNAC 2 cut(s) 205, 466
Hpy99I CGWCG 6 cut(s) 206, 209, 341, 359, 464, 797
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 3 cut(s) 613, 700, 815
HpyCH4IV ACGT 2 cut(s) 108, 462
HpyCH4V TGCA 2 cut(s) 348, 766
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 560, 865
HpyF3I CTNAG 6 cut(s) 118, 370, 641, 674, 830, 863
HpySE526I ACGT 2 cut(s) 108, 462
Hsp92II CATG 9 cut(s) 238, 348, 379, 433, 540, 580, 625, 708, 805
HspAI GCGC 4 cut(s) 112, 240, 277, 554
Kpn2I TCCGGA 1 cut(s) 545
Ksp22I TGATCA 1 cut(s) 693
Kzo9I GATC 6 cut(s) 166, 378, 432, 516, 693, 789
LmnI GCTCC 5 cut(s) 328, 336, 383, 499, 548
Lsp1109I GCAGC 1 cut(s) 846
MaeI CTAG 2 cut(s) 140, 447
MaeII ACGT 2 cut(s) 108, 462
MaeIII GTNAC 1 cut(s) 707
MalI GATC 6 cut(s) 168, 380, 434, 518, 695, 791
MboI GATC 6 cut(s) 166, 378, 432, 516, 693, 789
MboII GAAGA 6 cut(s) 73, 76, 107, 406, 503, 812
MhlI GDGCHC 3 cut(s) 333, 545, 836
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 2 cut(s) 477, 549
Mph1103I ATGCAT 1 cut(s) 350
MroI TCCGGA 1 cut(s) 545
MslI CAYNNNNRTG 1 cut(s) 887
MspA1I CMGCKG 1 cut(s) 559
MspI CCGG 7 cut(s) 407, 520, 546, 607, 629, 839, 854
MspR9I CCNGG 2 cut(s) 146, 596
MvaI CCWGG 2 cut(s) 146, 596
MvnI CGCG 3 cut(s) 114, 216, 336
MwoI GCNNNNNNNGC 3 cut(s) 392, 560, 865
NdeII GATC 6 cut(s) 166, 378, 432, 516, 693, 789
NlaIII CATG 9 cut(s) 238, 348, 379, 433, 540, 580, 625, 708, 805
NlaIV GGNNCC 6 cut(s) 8, 18, 163, 247, 385, 702
NmuCI GTSAC 1 cut(s) 707
NsiI ATGCAT 1 cut(s) 350
NspI RCATGY 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 323
PagI TCATGA 1 cut(s) 429
PcsI WCGNNNNNNNCGW 4 cut(s) 213, 321, 363, 480
PfeI GAWTC 3 cut(s) 480, 772, 850
PfoI TCCNGGA 1 cut(s) 594
PkrI GCNGC 8 cut(s) 79, 394, 439, 558, 572, 627, 782, 861
Ple19I CGATCG 1 cut(s) 792
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
PpuMI RGGWCCY 1 cut(s) 17
Psp124BI GAGCTC 2 cut(s) 333, 836
Psp5II RGGWCCY 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 144, 594
PspGI CCWGG 2 cut(s) 144, 594
PspN4I GGNNCC 6 cut(s) 8, 18, 163, 247, 385, 702
PspPI GGNCC 6 cut(s) 17, 148, 409, 522, 592, 609
PspPPI RGGWCCY 1 cut(s) 17
PvuI CGATCG 1 cut(s) 792
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 887
Rsr2I CGGWCCG 1 cut(s) 609
RsrII CGGWCCG 1 cut(s) 609
SacI GAGCTC 2 cut(s) 333, 836
SalI GTCGAC 1 cut(s) 203
SatI GCNGC 8 cut(s) 78, 393, 438, 557, 571, 626, 781, 860
Sau3AI GATC 6 cut(s) 166, 378, 432, 516, 693, 789
Sau96I GGNCC 6 cut(s) 17, 148, 409, 522, 592, 609
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 146, 596
SduI GDGCHC 3 cut(s) 333, 545, 836
Sfr274I CTCGAG 1 cut(s) 323
SgrDI CGTCGACG 1 cut(s) 203
SinI GGWCC 5 cut(s) 17, 409, 522, 592, 609
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SspMI CTAG 2 cut(s) 140, 447
SstI GAGCTC 2 cut(s) 333, 836
StyD4I CCNGG 2 cut(s) 144, 594
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 3 cut(s) 613, 700, 815
TaiI ACGT 2 cut(s) 111, 465
TaqI TCGA 9 cut(s) 204, 267, 273, 291, 324, 483, 510, 788, 795
TaqII GACCGA 1 cut(s) 163
TauI GCSGC 7 cut(s) 80, 395, 440, 559, 573, 628, 783
TfiI GAWTC 3 cut(s) 480, 772, 850
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 1 cut(s) 859
Tsp45I GTSAC 1 cut(s) 707
TspGWI ACGGA 2 cut(s) 355, 489
VpaK11BI GGWCC 5 cut(s) 17, 409, 522, 592, 609
XagI CCTNNNNNAGG 1 cut(s) 445
XceI RCATGY 1 cut(s) 625
XhoI CTCGAG 1 cut(s) 323
XmaJI CCTAGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 2 cut(s) 140, 447
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.