Rh2BG204300

Sigma factor sigB regulation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013318
Physical Location & Seq
Reverse (-)
18902034 .. 18904990
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG204300.1

Sequence Viewer

Length: 816 bp
ATGGGAATAGTGGCGGAAGCTCACAACGTTCGTGTTCTCGGGTCGGGTCAGCAGGTCATAGTCCTCGCCCACGGGTTCGGAACCGACCAGTCCGTCTGGAAGCACCTTGTCCCCCACCTGGTCGACGACTACCGGGTCGTCATGTACGACAACATGGGCGCCGGAACCACCAACCCCGAGTACTTTGATTTCGAGCGCTACTCCACGCTCGAGGGCTACGCGTACGACTTACTCGCCATCCTCGAAGAGTTTCAGGTCGAGAGTTGCATCTTTGTAGGTCACTCTGTTTCCGCCATGATTGGCACCATCGCCGCCGTCATGAGGCCGGAGCTTTTCTTTAAGCTCGTTATGATCGCCGCCTCTCCAAGGTATCTGAACGACACGGACTACTACGGAGGCTTCGAGGAAGAAGACCTACAACAACTCTTCGAAGCCATCCGCTCAAACTACAAGGCCTGGTGCGCCGGCTTTGCCCCGCTCGCCGTGGGTGGCGACATGGACTCGGTCGCCGTCCAAGAGTTTAGCCGGACCCTATTCAACATGCGACCCGACATAGCCCTCAGTGTGGCCCAGACCATTTTCCAAAGCGACACGAGACATATCATGGGCATGGTCACGGTGCCTTGCCACATACTCCAGAGTGTTAAGGACTTGGCGGTGCCGGTGGTGGTGTCGGAGTACCTGCACCAGAATTTGGGGGGTGTGTCAATCGTGGAGGTGATGTCATCGGACGGGCATCTCCCTCAGCTGAGCTCGCCGGATATTGTGATTCCCGTGCTGCTCCGGCACATTACACATGATATTGCGACGCGATGA

Protein Analysis

271

Amino Acids

30.05

Weight (kDa)

4.84

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 17 - 229 6.7e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 19 - 252 4.2e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 20 - 257 6.7e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37470
fragaria_vesca FvH4_1g17110
malus_domestica MD02G1181300.v1.1 MD02G1181500.v1.1
prunus_persica Prupe.6G225000_v2.0.a1
pyrus_communis pycom02g14720 pycom02g14730
rosa_chinensis RchiOBHm_Chr2g0107281
rosa_laevigata RLG00000017567
rosa_multiflora Rmu_co8389809.1_g000001 Rmu_sc0006416.1_g000017
rosa_roxburghii Rroxscaffold_2G00136470
rosa_rugosa Rorug02G0141400
rosa_samantha Rh2AG192400 Rh2BG204300 Rh2CG197600 Rh2DG199600
rosa_wichuraiana Rw2G015220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 92, 134
Acc36I ACCTGC 2 cut(s) 43, 690
AccB1I GGYRCC 4 cut(s) 158, 302, 619, 658
AccB7I CCANNNNNTGG 1 cut(s) 694
AccBSI CCGCTC 2 cut(s) 441, 478
AccI GTMKAC 1 cut(s) 123
AccII CGCG 2 cut(s) 221, 811
AciI CCGC 7 cut(s) 14, 291, 312, 357, 439, 476, 656
AclI AACGTT 1 cut(s) 27
AcsI RAATTY 1 cut(s) 691
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 4 cut(s) 146, 182, 224, 680
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 5 cut(s) 118, 321, 366, 565, 694
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 1 cut(s) 538
AjnI CCWGG 2 cut(s) 117, 455
AluBI AGCT 5 cut(s) 20, 331, 343, 748, 753
AluI AGCT 5 cut(s) 20, 331, 343, 748, 753
Alw21I GWGCWC 1 cut(s) 755
Alw26I GTCTC 1 cut(s) 589
Ama87I CYCGRG 3 cut(s) 38, 176, 209
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 3 cut(s) 323, 453, 567
ApeKI GCWGC 1 cut(s) 778
ApoI RAATTY 1 cut(s) 691
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 3 cut(s) 161, 198, 464
AspS9I GGNCC 2 cut(s) 528, 568
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 730
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 3 cut(s) 38, 176, 209
AvaII GGWCC 1 cut(s) 528
BanI GGYRCC 4 cut(s) 158, 302, 619, 658
BanII GRGCYC 1 cut(s) 755
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
BauI CACGAG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 755
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 1 cut(s) 765
BccI CCATC 3 cut(s) 245, 314, 443
BceAI ACGGC 3 cut(s) 299, 467, 494
BciT130I CCWGG 2 cut(s) 119, 457
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 589
BfoI RGCGCY 2 cut(s) 162, 199
BfuAI ACCTGC 2 cut(s) 43, 690
BisI GCNGC 3 cut(s) 312, 357, 779
BlpI GCTNAGC 1 cut(s) 749
BlsI GCNGC 3 cut(s) 313, 358, 780
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 3 cut(s) 119, 134, 457
Bme18I GGWCC 1 cut(s) 528
BmeT110I CYCGRG 3 cut(s) 38, 176, 209
BmgT120I GGNCC 2 cut(s) 528, 568
BmiI GGNNCC 7 cut(s) 82, 160, 166, 304, 530, 621, 660
BmrFI CCNGG 3 cut(s) 119, 134, 457
BmsI GCATC 2 cut(s) 276, 745
BpiI GAAGAC 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 185, 217
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 749
Bpu14I TTCGAA 1 cut(s) 429
BpuMI CCSGG 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 70, 365, 483
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 5 cut(s) 118, 321, 366, 565, 694
Bse118I RCCGGY 2 cut(s) 464, 661
Bse1I ACTGG 1 cut(s) 88
BseBI CCWGG 2 cut(s) 119, 457
BseDI CCNNGG 3 cut(s) 70, 365, 483
BseGI GGATG 2 cut(s) 237, 435
BseLI CCNNNNNNNGG 5 cut(s) 118, 321, 366, 565, 694
BseMII CTCAG 3 cut(s) 574, 740, 758
BseNI ACTGG 1 cut(s) 88
BseXI GCAGC 1 cut(s) 765
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 2 cut(s) 221, 811
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 3 cut(s) 325, 455, 569
BshNI GGYRCC 4 cut(s) 158, 302, 619, 658
BsiEI CGRYCG 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 755
BsiHKCI CYCGRG 3 cut(s) 38, 176, 209
BsiSI CCGG 8 cut(s) 133, 162, 326, 465, 526, 662, 758, 784
BsiWI CGTACG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 5 cut(s) 118, 321, 366, 565, 694
BsmAI GTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 3 cut(s) 325, 455, 569
BsoBI CYCGRG 3 cut(s) 38, 176, 209
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 755
Bsp143I GATC 1 cut(s) 351
Bsp1720I GCTNAGC 1 cut(s) 749
BspACI CCGC 7 cut(s) 14, 291, 312, 357, 439, 476, 656
BspANI GGCC 3 cut(s) 325, 455, 569
BspCNI CTCAG 3 cut(s) 573, 741, 757
BspFNI CGCG 2 cut(s) 221, 811
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 7 cut(s) 82, 160, 166, 304, 530, 621, 660
BspMI ACCTGC 2 cut(s) 43, 690
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 4 cut(s) 158, 302, 619, 658
BsrBI CCGCTC 2 cut(s) 441, 478
BsrFI RCCGGY 2 cut(s) 464, 661
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 2 cut(s) 464, 661
BssECI CCNNGG 3 cut(s) 70, 365, 483
BssMI GATC 1 cut(s) 351
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 592
BssT1I CCWWGG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 592
Bst2UI CCWGG 2 cut(s) 119, 457
Bst4CI ACNGT 1 cut(s) 619
Bst6I CTCTTC 2 cut(s) 240, 431
BstACI GRCGYC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 466, 480, 755
BstDEI CTNAG 3 cut(s) 560, 744, 749
BstDSI CCRYGG 2 cut(s) 70, 483
BstENI CCTNNNNNAGG 1 cut(s) 364
BstF5I GGATG 2 cut(s) 237, 435
BstFNI CGCG 2 cut(s) 221, 811
BstH2I RGCGCY 2 cut(s) 162, 199
BstHHI GCGC 3 cut(s) 161, 198, 464
BstKTI GATC 1 cut(s) 354
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 1 cut(s) 351
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 5 cut(s) 461, 470, 479, 754, 784
BstNI CCWGG 2 cut(s) 119, 457
BstNSI RCATGY 1 cut(s) 544
BstSCI CCNGG 3 cut(s) 117, 132, 455
BstUI CGCG 2 cut(s) 221, 811
BstV1I GCAGC 1 cut(s) 765
BstV2I GAAGAC 1 cut(s) 417
BsuRI GGCC 3 cut(s) 325, 455, 569
BtgI CCRYGG 2 cut(s) 70, 483
BtgZI GCGATG 1 cut(s) 292
BtsCI GGATG 2 cut(s) 237, 435
BtsIMutI CAGTG 1 cut(s) 568
BveI ACCTGC 2 cut(s) 43, 690
Cac8I GCNNGC 3 cut(s) 466, 480, 755
CciI TCATGA 1 cut(s) 318
CfoI GCGC 3 cut(s) 161, 198, 464
Cfr10I RCCGGY 2 cut(s) 464, 661
Cfr13I GGNCC 2 cut(s) 528, 568
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 4 cut(s) 145, 181, 223, 679
CviAII CATG 9 cut(s) 142, 154, 295, 319, 496, 541, 604, 610, 797
CviQI GTAC 4 cut(s) 145, 181, 223, 679
DdeI CTNAG 3 cut(s) 560, 744, 749
DinI GGCGCC 1 cut(s) 160
DpnI GATC 1 cut(s) 353
DpnII GATC 1 cut(s) 351
DrdI GACNNNNNNGTC 2 cut(s) 92, 134
DseDI GACNNNNNNGTC 2 cut(s) 92, 134
Eam1104I CTCTTC 2 cut(s) 240, 431
EarI CTCTTC 2 cut(s) 240, 431
EciI GGCGGA 2 cut(s) 29, 280
Ecl136II GAGCTC 1 cut(s) 753
Eco130I CCWWGG 1 cut(s) 365
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 528
Eco47III AGCGCT 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 753
Eco88I CYCGRG 3 cut(s) 38, 176, 209
EcoICRI GAGCTC 1 cut(s) 753
EcoNI CCTNNNNNAGG 1 cut(s) 364
EcoRII CCWGG 2 cut(s) 117, 455
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 755
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 9 cut(s) 145, 157, 298, 322, 499, 544, 607, 613, 800
FaqI GGGAC 1 cut(s) 95
FatI CATG 9 cut(s) 141, 153, 294, 318, 495, 540, 603, 609, 796
FauI CCCGC 1 cut(s) 483
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 312, 357, 779
FokI GGATG 2 cut(s) 224, 422
FriOI GRGCYC 1 cut(s) 755
Fsp4HI GCNGC 3 cut(s) 312, 357, 779
GlaI GCGC 3 cut(s) 160, 197, 463
GluI GCNGC 3 cut(s) 312, 357, 779
GsuI CTGGAG 1 cut(s) 620
HaeII RGCGCY 2 cut(s) 162, 199
HaeIII GGCC 3 cut(s) 325, 455, 569
HapII CCGG 8 cut(s) 133, 162, 326, 465, 526, 662, 758, 784
HhaI GCGC 3 cut(s) 161, 198, 464
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 9 cut(s) 145, 157, 298, 322, 499, 544, 607, 613, 800
Hin6I GCGC 3 cut(s) 159, 196, 462
HinP1I GCGC 3 cut(s) 159, 196, 462
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 2 cut(s) 500, 769
HpaII CCGG 8 cut(s) 133, 162, 326, 465, 526, 662, 758, 784
HphI GGTGA 1 cut(s) 730
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 80, 375, 676, 730
Hpy188III TCNNGA 4 cut(s) 97, 259, 319, 637
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 2 cut(s) 128, 811
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 267, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 470, 479, 754, 784
HpyF3I CTNAG 3 cut(s) 560, 744, 749
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 9 cut(s) 145, 157, 298, 322, 499, 544, 607, 613, 800
HspAI GCGC 3 cut(s) 159, 196, 462
KasI GGCGCC 1 cut(s) 158
KroI GCCGGC 1 cut(s) 464
KroNI GCCGGC 1 cut(s) 466
Kzo9I GATC 1 cut(s) 351
LmnI GCTCC 2 cut(s) 328, 786
Lsp1109I GCAGC 1 cut(s) 765
LweI GCATC 2 cut(s) 276, 745
MabI ACCWGGT 1 cut(s) 117
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 278, 613
MalI GATC 1 cut(s) 353
MbiI CCGCTC 2 cut(s) 441, 478
MboI GATC 1 cut(s) 351
MboII GAAGA 4 cut(s) 257, 418, 419, 422
MhlI GDGCHC 1 cut(s) 755
MluCI AATT 1 cut(s) 691
MluI ACGCGT 1 cut(s) 219
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 1 cut(s) 494
MmeI TCCRAC 1 cut(s) 654
MroNI GCCGGC 1 cut(s) 464
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 2 cut(s) 339, 645
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 748
MspI CCGG 8 cut(s) 133, 162, 326, 465, 526, 662, 758, 784
MspR9I CCNGG 3 cut(s) 119, 134, 457
MvaI CCWGG 2 cut(s) 119, 457
MvnI CGCG 2 cut(s) 221, 811
MwoI GCNNNNNNNGC 5 cut(s) 461, 470, 479, 754, 784
NaeI GCCGGC 1 cut(s) 466
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 1 cut(s) 134
NdeII GATC 1 cut(s) 351
NgoMIV GCCGGC 1 cut(s) 464
NlaIII CATG 9 cut(s) 145, 157, 298, 322, 499, 544, 607, 613, 800
NlaIV GGNNCC 7 cut(s) 82, 160, 166, 304, 530, 621, 660
NmuCI GTSAC 2 cut(s) 278, 613
NspI RCATGY 1 cut(s) 544
NspV TTCGAA 1 cut(s) 429
PaeR7I CTCGAG 1 cut(s) 209
PagI TCATGA 1 cut(s) 318
PceI AGGCCT 1 cut(s) 455
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 240, 399
PdiI GCCGGC 1 cut(s) 466
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 769
Pfl23II CGTACG 1 cut(s) 222
PflFI GACNNNGTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 694
PkrI GCNGC 3 cut(s) 313, 358, 780
PleI GAGTC 1 cut(s) 494
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 755
Psp1406I AACGTT 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 117, 455
PspGI CCWGG 2 cut(s) 117, 455
PspLI CGTACG 1 cut(s) 222
PspN4I GGNNCC 7 cut(s) 82, 160, 166, 304, 530, 621, 660
PspPI GGNCC 2 cut(s) 528, 568
PspXI VCTCGAGB 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 748
RsaI GTAC 4 cut(s) 146, 182, 224, 680
RsaNI GTAC 4 cut(s) 145, 181, 223, 679
RseI CAYNNNNRTG 1 cut(s) 608
SacI GAGCTC 1 cut(s) 755
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 339, 645
SatI GCNGC 3 cut(s) 312, 357, 779
Sau3AI GATC 1 cut(s) 351
Sau96I GGNCC 2 cut(s) 528, 568
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 3 cut(s) 119, 134, 457
SduI GDGCHC 1 cut(s) 755
SexAI ACCWGGT 1 cut(s) 117
SfaNI GCATC 2 cut(s) 276, 745
SfoI GGCGCC 1 cut(s) 160
Sfr274I CTCGAG 1 cut(s) 209
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 1 cut(s) 528
SlaI CTCGAG 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 691
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 7 cut(s) 14, 291, 312, 357, 439, 476, 656
SspDI GGCGCC 1 cut(s) 158
SstI GAGCTC 1 cut(s) 755
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 117, 132, 455
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 1 cut(s) 30
TaqI TCGA 7 cut(s) 123, 192, 210, 243, 258, 402, 429
TaqII GACCGA 1 cut(s) 493
TasI AATT 1 cut(s) 691
TatI WGTACW 1 cut(s) 180
TauI GCSGC 2 cut(s) 314, 359
TfiI GAWTC 1 cut(s) 769
Tru1I TTAA 2 cut(s) 339, 645
Tru9I TTAA 2 cut(s) 339, 645
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 2 cut(s) 278, 613
TseI GCWGC 1 cut(s) 778
Tsp45I GTSAC 2 cut(s) 278, 613
TspGWI ACGGA 3 cut(s) 82, 398, 408
TspRI CASTG 1 cut(s) 568
Tth111I GACNNNGTC 1 cut(s) 503
Van91I CCANNNNNTGG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 528
XagI CCTNNNNNAGG 1 cut(s) 364
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 544
XhoI CTCGAG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 249
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.