Rmu_co8389809.1_g000001

Sigma factor sigB regulation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8389809.1
OG0013318
Physical Location & Seq
Forward (+)
26 .. 834
809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8389809.1_g000001.1.cds

Sequence Viewer

Length: 498 bp
ctgaacgacgcggactactacggaggcttcgaggaagaagacctacaacaactcttcgaagccatccgctcaaactacaaggcctggtgcgccggctttgccccgctcgccgtgggtggcgacatggactcggtcgccgtccaagaatttagccggaccctattcaacatgcgacccgacatagccctcagtgtggcccagaccattttccaaagcgacacgagacatatcattggcatggtcacggtgccttgccacatactccagagtgttaaggacttggcggtgccggtggtggtgtcggagtacctgcaccagaatttggggggtgagtcgatcgtggaggtgatgtcatcggacgggcatctccctcagctgagctcgccggatattgtgattcccgtccctttgttggatgtcgtgttcaaagtgaatggactcacatgtatgaaggtgggaagatttgaacgggtggtaataatgtattttcagttgtga

Protein Analysis

165

Amino Acids

18.28

Weight (kDa)

4.61

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37470
fragaria_vesca FvH4_1g17110
malus_domestica MD02G1181300.v1.1 MD02G1181500.v1.1
prunus_persica Prupe.6G225000_v2.0.a1
pyrus_communis pycom02g14720 pycom02g14730
rosa_chinensis RchiOBHm_Chr2g0107281
rosa_laevigata RLG00000017567
rosa_multiflora Rmu_co8389809.1_g000001 Rmu_sc0006416.1_g000017
rosa_roxburghii Rroxscaffold_2G00136470
rosa_rugosa Rorug02G0141400
rosa_samantha Rh2AG192400 Rh2BG204300 Rh2CG197600 Rh2DG199600
rosa_wichuraiana Rw2G015220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 318
AccB1I GGYRCC 2 cut(s) 247, 286
AccB7I CCANNNNNTGG 1 cut(s) 322
AccBSI CCGCTC 2 cut(s) 69, 106
AccII CGCG 1 cut(s) 11
AciI CCGC 4 cut(s) 11, 67, 104, 284
AcsI RAATTY 2 cut(s) 146, 319
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 3 cut(s) 193, 322, 412
AflIII ACRYGT 1 cut(s) 443
AgsI TTSAA 3 cut(s) 166, 427, 467
AjnI CCWGG 1 cut(s) 83
AloI GAACNNNNNNTCC 2 cut(s) 407, 439
AluBI AGCT 2 cut(s) 376, 381
AluI AGCT 2 cut(s) 376, 381
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 1 cut(s) 217
AoxI GGCC 2 cut(s) 81, 195
ApoI RAATTY 2 cut(s) 146, 319
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 156, 196
AsuHPI GGTGA 2 cut(s) 341, 358
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 156
BanI GGYRCC 2 cut(s) 247, 286
BanII GRGCYC 1 cut(s) 383
BarI GAAGNNNNNNTAC 2 cut(s) 27, 59
BauI CACGAG 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 372
BccI CCATC 1 cut(s) 71
BceAI ACGGC 2 cut(s) 95, 122
BciT130I CCWGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 217
BfuAI ACCTGC 1 cut(s) 318
BlpI GCTNAGC 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 156
BmgT120I GGNCC 2 cut(s) 156, 196
BmiI GGNNCC 3 cut(s) 158, 249, 288
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 248
Bpu10I CCTNAGC 1 cut(s) 372
Bpu1102I GCTNAGC 1 cut(s) 377
Bpu14I TTCGAA 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 111
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 322, 412
Bse118I RCCGGY 2 cut(s) 92, 289
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 63, 421
BseLI CCNNNNNNNGG 3 cut(s) 193, 322, 412
BseMII CTCAG 3 cut(s) 202, 368, 386
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 11
Bsh1285I CGRYCG 2 cut(s) 135, 339
BshFI GGCC 2 cut(s) 83, 197
BshNI GGYRCC 2 cut(s) 247, 286
BsiEI CGRYCG 2 cut(s) 135, 339
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 4 cut(s) 93, 154, 290, 386
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 3 cut(s) 193, 322, 412
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 2 cut(s) 83, 197
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 1 cut(s) 336
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 4 cut(s) 11, 67, 104, 284
BspANI GGCC 2 cut(s) 83, 197
BspCNI CTCAG 3 cut(s) 201, 369, 385
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 158, 249, 288
BspMI ACCTGC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 2 cut(s) 247, 286
BsrBI CCGCTC 2 cut(s) 69, 106
BsrFI RCCGGY 2 cut(s) 92, 289
BssAI RCCGGY 2 cut(s) 92, 289
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 59
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 94, 108, 383
BstDEI CTNAG 3 cut(s) 188, 372, 377
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 2 cut(s) 63, 421
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 339
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 1 cut(s) 336
BstMCI CGRYCG 2 cut(s) 135, 339
BstMWI GCNNNNNNNGC 4 cut(s) 89, 98, 107, 382
BstNI CCWGG 1 cut(s) 85
BstNSI RCATGY 2 cut(s) 172, 447
BstSCI CCNGG 1 cut(s) 83
BstUI CGCG 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 83, 197
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 63, 421
BtsIMutI CAGTG 1 cut(s) 196
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 3 cut(s) 94, 108, 383
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 2 cut(s) 92, 289
Cfr13I GGNCC 2 cut(s) 156, 196
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 124, 169, 238, 444
CviJI RGCY 9 cut(s) 27, 62, 83, 96, 153, 185, 197, 376, 381
CviKI_1 RGCY 9 cut(s) 27, 62, 83, 96, 153, 185, 197, 376, 381
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 3 cut(s) 188, 372, 377
DpnI GATC 1 cut(s) 338
DpnII GATC 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 381
Eco147I AGGCCT 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 383
FaeI CATG 4 cut(s) 127, 172, 241, 447
FaiI YATR 8 cut(s) 125, 170, 182, 228, 239, 260, 445, 449
FaqI GGGAC 1 cut(s) 389
FatI CATG 4 cut(s) 123, 168, 237, 443
FauI CCCGC 1 cut(s) 111
FokI GGATG 2 cut(s) 50, 428
FriOI GRGCYC 1 cut(s) 383
GlaI GCGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 2 cut(s) 83, 197
HapII CCGG 4 cut(s) 93, 154, 290, 386
HgaI GACGC 1 cut(s) 17
HhaI GCGC 1 cut(s) 92
Hin1II CATG 4 cut(s) 127, 172, 241, 447
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HinfI GANTC 4 cut(s) 128, 332, 397, 438
HpaII CCGG 4 cut(s) 93, 154, 290, 386
HphI GGTGA 2 cut(s) 341, 358
Hpy188I TCNGA 2 cut(s) 304, 358
Hpy188III TCNNGA 1 cut(s) 265
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 1 cut(s) 445
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 98, 107, 382
HpyF3I CTNAG 3 cut(s) 188, 372, 377
Hsp92II CATG 4 cut(s) 127, 172, 241, 447
HspAI GCGC 1 cut(s) 90
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 336
LweI GCATC 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 241
MalI GATC 1 cut(s) 338
MbiI CCGCTC 2 cut(s) 69, 106
MboI GATC 1 cut(s) 336
MboII GAAGA 4 cut(s) 46, 47, 50, 471
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 2 cut(s) 146, 319
MlyI GAGTC 3 cut(s) 122, 341, 432
MmeI TCCRAC 2 cut(s) 282, 393
MnlI CCTC 5 cut(s) 17, 25, 197, 337, 381
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 2 cut(s) 236, 446
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 4 cut(s) 93, 154, 290, 386
MspR9I CCNGG 1 cut(s) 85
MvaI CCWGG 1 cut(s) 85
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 4 cut(s) 89, 98, 107, 382
NaeI GCCGGC 1 cut(s) 94
NdeII GATC 1 cut(s) 336
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 4 cut(s) 127, 172, 241, 447
NlaIV GGNNCC 3 cut(s) 158, 249, 288
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 2 cut(s) 172, 447
NspV TTCGAA 1 cut(s) 57
PceI AGGCCT 1 cut(s) 83
PciI ACATGT 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 397
PflFI GACNNNGTC 1 cut(s) 131
PflMI CCANNNNNTGG 1 cut(s) 322
Ple19I CGATCG 1 cut(s) 339
PleI GAGTC 3 cut(s) 122, 340, 432
PpsI GAGTC 3 cut(s) 122, 340, 432
PscI ACATGT 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 3 cut(s) 158, 249, 288
PspPI GGNCC 2 cut(s) 156, 196
PsyI GACNNNGTC 1 cut(s) 131
PvuI CGATCG 1 cut(s) 339
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 236, 446
SacI GAGCTC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 273
Sau3AI GATC 1 cut(s) 336
Sau96I GGNCC 2 cut(s) 156, 196
SchI GAGTC 3 cut(s) 122, 341, 432
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 1 cut(s) 383
SetI ASST 6 cut(s) 45, 312, 348, 378, 383, 456
SfaNI GCATC 1 cut(s) 373
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 1 cut(s) 156
SmiMI CAYNNNNRTG 2 cut(s) 236, 446
Sse9I AATT 2 cut(s) 146, 319
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 4 cut(s) 11, 67, 104, 284
SstI GAGCTC 1 cut(s) 383
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 83
TaaI ACNGT 1 cut(s) 247
TaqI TCGA 3 cut(s) 30, 57, 335
TaqII GACCGA 1 cut(s) 121
TasI AATT 2 cut(s) 146, 319
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 196
Tth111I GACNNNGTC 1 cut(s) 131
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 2 cut(s) 146, 319
XceI RCATGY 2 cut(s) 172, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.