MD02G1183900.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009502
Physical Location & Seq
Reverse (-)
16681557 .. 16683335
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1183900.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGTTGAAGACGAAGATGGTGTTCTTCTTTCATCTTCTATTTCTCCTTTCTTTTGGTGTTTCTGGTGAAAGGCAAGACTCCATTGTTTCTGTAAAATCTGGAGATCTCAGTGAGGTTGCAAGCAGCATTTTAAAGGCAGAAACTTGGCTCAAAACCCACGTCCTTGCTCACTACCCTTCCGCCCCCATCACCGCCATTGTCGTCGGCGGCGTTGGCGGCGTCCCTCTTCTTCTCTGCCGGAAAGAGCAAGAACACACTCTCACTCTCATCCTCCCGGCCGTCAAGAACTTGCACCACTCCCTCACACGGTGGGGCCTCCAGGAACACATCAAACTCTCACCCGCCTTCGCTCCTTCTTGTTTCGTCAGAAGCTCCTATATCTTTGGGAAATTTGTGAGGCCTGTCCTAGATTTTCTCAAGGGAGCATCTCAAACTGTCTCTCCAACGTTGGTGTCTTCTCACTCCGAAAGCCTTGAGGACTTTATCGGCGTTAGCTTTAACTTTGACGATTATACTGTCGTTCTTGACAACCCCGGAGAGAGAAAACCCAGCGGCAGAAAGCTCTCTGCGGACCCCTTTCCAGCGAGACCGAAGCCATTGCCTAAAATTTCAGAAACCCCACTTCACTCCACTGTTGGTCTCTCTGTTCCCGCCAATGTGGCTAAAAATCCTCATCCTCCATTGCCCCAAACTGCCTCTCCGCCGCTCCAAGTTCCTTCGCCACAACCCCAAAGTGCCGTCTCTCCGCCACCACTCCTGCCTTCCCCAGAGGCACAAACAGCCTCTCCACCACCACCCCAGATAGCTTCTCCACCAGATACGGGGTTCTCCTCAGCCCCAGAAAACCCTCCTTTTGTTGATGATTCCCCTCCGCCGTCCCCTGCCACAATTCCACCCTGCAGGCCGACACACGTGCCACCTCCTGCCCCAGCCCCTCAGACTGCAGTGCACAACAAGCTGTGGTGTGTGGCCAAGCCCACTGTTCCTTCAGACACTCTGCAGGTGGCAATGGACTACGCCTGCGGAGACGGCGGTGCTGACTGCTCGGAGATCATGTCGGATGGCAACTGCTACAGCCCTGATTCTGTTGTGGCACATGCTTCCTACGCCTTCAACAGCTACTGGCAGAAGAACAAGAAGACCGGAGGGACTTGCAGCTTCGATGGCACTGCAATGTTGATTAACAATGATCCAAGTTATCTTCAGTGTCGATTCATTCTCACCTGA

Protein Analysis

409

Amino Acids

43.66

Weight (kDa)

6.66

Isoelectric Point (pI)

66.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 320 - 392 4.7e-24 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 991
Acc36I ACCTGC 1 cut(s) 991
AccBSI CCGCTC 1 cut(s) 706
AclI AACGTT 1 cut(s) 446
AclWI GGATC 1 cut(s) 1184
AcoI YGGCCR 2 cut(s) 276, 969
AcsI RAATTY 2 cut(s) 389, 606
AcuI CTGAAG 2 cut(s) 972, 1187
AcvI CACGTG 1 cut(s) 913
AcyI GRCGYC 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 309
AfiI CCNNNNNNNGG 2 cut(s) 306, 821
AflIII ACRYGT 1 cut(s) 910
AgsI TTSAA 2 cut(s) 7, 1114
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 7 cut(s) 372, 495, 562, 806, 958, 1119, 1158
AluI AGCT 7 cut(s) 372, 495, 562, 806, 958, 1119, 1158
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 5 cut(s) 442, 580, 644, 745, 1020
Alw44I GTGCAC 1 cut(s) 947
AlwI GGATC 1 cut(s) 1184
AlwNI CAGNNNCTG 1 cut(s) 1122
AoxI GGCC 5 cut(s) 276, 313, 398, 902, 969
ApaLI GTGCAC 1 cut(s) 947
ApeKI GCWGC 2 cut(s) 123, 1155
ApoI RAATTY 2 cut(s) 389, 606
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
AspS9I GGNCC 2 cut(s) 313, 571
AsuC2I CCSGG 2 cut(s) 275, 534
AsuHPI GGTGA 4 cut(s) 77, 181, 330, 1213
AvaII GGWCC 1 cut(s) 571
BaeGI GKGCMC 1 cut(s) 951
BalI TGGCCA 1 cut(s) 971
BbrPI CACGTG 1 cut(s) 913
BbsI GAAGAC 3 cut(s) 14, 447, 1145
Bbv12I GWGCWC 1 cut(s) 951
BbvCI CCTCAGC 1 cut(s) 832
BbvI GCAGC 2 cut(s) 135, 1167
BccI CCATC 4 cut(s) 10, 194, 1055, 1157
BceAI ACGGC 4 cut(s) 263, 722, 859, 1045
BcgI CGANNNNNNTGC 6 cut(s) 580, 614, 895, 929, 1151, 1185
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 2 cut(s) 275, 534
BcoDI GTCTC 5 cut(s) 442, 580, 644, 745, 1020
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 4 cut(s) 898, 942, 998, 1072
BfuAI ACCTGC 1 cut(s) 991
BglI GCCNNNNNGGC 1 cut(s) 659
BglII AGATCT 1 cut(s) 103
BisI GCNGC 6 cut(s) 124, 208, 217, 553, 704, 1156
BlsI GCNGC 6 cut(s) 125, 209, 218, 554, 705, 1157
Bme1390I CCNGG 3 cut(s) 275, 320, 534
Bme18I GGWCC 1 cut(s) 571
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 313, 571
BmiI GGNNCC 2 cut(s) 314, 573
BmrFI CCNGG 3 cut(s) 275, 320, 534
BmsI GCATC 1 cut(s) 434
BpiI GAAGAC 3 cut(s) 14, 447, 1145
BpmI CTGGAG 2 cut(s) 120, 302
Bpu10I CCTNAGC 1 cut(s) 832
BpuEI CTTGAG 2 cut(s) 401, 494
BpuMI CCSGG 2 cut(s) 275, 534
BsaAI YACGTR 1 cut(s) 913
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 2 cut(s) 580, 644
BsaJI CCNNGG 1 cut(s) 532
BsaWI WCCGGW 1 cut(s) 1142
BsaXI ACNNNNNCTCC 8 cut(s) 424, 454, 682, 712, 769, 799, 1040, 1070
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 821
Bse1I ACTGG 1 cut(s) 1127
Bse3DI GCAATG 4 cut(s) 596, 680, 1014, 1179
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 3 cut(s) 267, 673, 1066
BseLI CCNNNNNNNGG 2 cut(s) 306, 821
BseMI GCAATG 4 cut(s) 596, 680, 1014, 1179
BseMII CTCAG 3 cut(s) 121, 846, 950
BseNI ACTGG 1 cut(s) 1127
BseRI GAGGAG 1 cut(s) 820
BseSI GKGCMC 1 cut(s) 951
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 2 cut(s) 135, 1167
BseYI CCCAGC 2 cut(s) 548, 928
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 5 cut(s) 278, 315, 400, 904, 971
BsiEI CGRYCG 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 4 cut(s) 238, 275, 534, 1143
BslFI GGGAC 3 cut(s) 206, 862, 1162
BslI CCNNNNNNNGG 2 cut(s) 306, 821
BsmAI GTCTC 5 cut(s) 442, 580, 644, 745, 1020
BsmBI CGTCTC 2 cut(s) 745, 1020
BsmFI GGGAC 3 cut(s) 206, 862, 1162
BsnI GGCC 5 cut(s) 278, 315, 400, 904, 971
Bso31I GGTCTC 2 cut(s) 580, 644
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 3 cut(s) 103, 1050, 1189
BspANI GGCC 5 cut(s) 278, 315, 400, 904, 971
BspCNI CTCAG 3 cut(s) 120, 845, 949
BspLI GGNNCC 2 cut(s) 314, 573
BspMAI CTGCAG 3 cut(s) 902, 946, 1002
BspMI ACCTGC 1 cut(s) 991
BspPI GGATC 1 cut(s) 1184
BspTNI GGTCTC 2 cut(s) 580, 644
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 4 cut(s) 596, 680, 1014, 1179
BsrI ACTGG 1 cut(s) 1127
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 3 cut(s) 103, 1050, 1189
BssNI GRCGYC 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 5 cut(s) 309, 436, 517, 634, 982
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 913
BstC8I GCNNGC 3 cut(s) 121, 902, 1021
BstDEI CTNAG 3 cut(s) 107, 832, 936
BstF5I GGATG 3 cut(s) 267, 673, 1066
BstKTI GATC 3 cut(s) 106, 1053, 1192
BstMAI GTCTC 5 cut(s) 442, 580, 644, 745, 1020
BstMBI GATC 3 cut(s) 103, 1050, 1189
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 8 cut(s) 213, 216, 659, 779, 955, 1029, 1106, 1164
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 1100
BstSCI CCNGG 3 cut(s) 273, 318, 532
BstSFI CTRYAG 4 cut(s) 898, 942, 998, 1072
BstSLI GKGCMC 1 cut(s) 951
BstV1I GCAGC 2 cut(s) 135, 1167
BstV2I GAAGAC 3 cut(s) 14, 447, 1145
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BstZI CGGCCG 1 cut(s) 276
BsuRI GGCC 5 cut(s) 278, 315, 400, 904, 971
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 3 cut(s) 267, 673, 1066
BtsI GCAGTG 2 cut(s) 951, 1167
BtsIMutI CAGTG 6 cut(s) 115, 630, 951, 978, 1167, 1211
BveI ACCTGC 1 cut(s) 991
Cac8I GCNNGC 3 cut(s) 121, 902, 1021
CaiI CAGNNNCTG 1 cut(s) 1122
Cfr13I GGNCC 2 cut(s) 313, 571
CseI GACGC 1 cut(s) 208
CviAII CATG 2 cut(s) 1054, 1097
DdeI CTNAG 3 cut(s) 107, 832, 936
DpnI GATC 3 cut(s) 105, 1052, 1191
DpnII GATC 3 cut(s) 103, 1050, 1189
DraI TTTAAA 1 cut(s) 132
DraIII CACNNNGTG 1 cut(s) 309
EaeI YGGCCR 2 cut(s) 276, 969
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 4 cut(s) 169, 690, 735, 861
EclXI CGGCCG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 400
Eco31I GGTCTC 2 cut(s) 580, 644
Eco47I GGWCC 1 cut(s) 571
Eco52I CGGCCG 1 cut(s) 276
Eco57I CTGAAG 2 cut(s) 972, 1187
Eco72I CACGTG 1 cut(s) 913
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 318
Esp3I CGTCTC 2 cut(s) 745, 1020
FaeI CATG 2 cut(s) 1057, 1100
FaiI YATR 4 cut(s) 378, 513, 1055, 1098
FaqI GGGAC 3 cut(s) 206, 862, 1162
FatI CATG 2 cut(s) 1053, 1096
FauI CCCGC 2 cut(s) 349, 658
Fnu4HI GCNGC 6 cut(s) 124, 208, 217, 553, 704, 1156
FokI GGATG 3 cut(s) 254, 660, 1073
Fsp4HI GCNGC 6 cut(s) 124, 208, 217, 553, 704, 1156
FspBI CTAG 1 cut(s) 407
GluI GCNGC 6 cut(s) 124, 208, 217, 553, 704, 1156
GsaI CCCAGC 2 cut(s) 552, 932
GsuI CTGGAG 2 cut(s) 120, 302
HaeIII GGCC 5 cut(s) 278, 315, 400, 904, 971
HapII CCGG 4 cut(s) 238, 275, 534, 1143
HgaI GACGC 1 cut(s) 208
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 2 cut(s) 1057, 1100
HinfI GANTC 4 cut(s) 77, 863, 1082, 1212
HpaII CCGG 4 cut(s) 238, 275, 534, 1143
HphI GGTGA 4 cut(s) 77, 181, 330, 1213
Hpy166II GTNNAC 1 cut(s) 949
Hpy188I TCNGA 7 cut(s) 368, 466, 613, 939, 991, 1048, 1060
Hpy188III TCNNGA 3 cut(s) 99, 283, 524
Hpy8I GTNNAC 1 cut(s) 949
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 7 cut(s) 186, 355, 363, 726, 771, 996, 1120
HpyCH4III ACNGT 5 cut(s) 309, 436, 517, 634, 982
HpyCH4IV ACGT 3 cut(s) 159, 446, 912
HpyCH4V TGCA 8 cut(s) 119, 292, 900, 944, 949, 1000, 1155, 1172
HpyF10VI GCNNNNNNNGC 8 cut(s) 213, 216, 659, 779, 955, 1029, 1106, 1164
HpyF3I CTNAG 3 cut(s) 107, 832, 936
HpySE526I ACGT 3 cut(s) 159, 446, 912
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 2 cut(s) 1057, 1100
Kzo9I GATC 3 cut(s) 103, 1050, 1189
LmnI GCTCC 4 cut(s) 355, 377, 422, 711
Lsp1109I GCAGC 2 cut(s) 135, 1167
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 407
MaeII ACGT 3 cut(s) 159, 446, 912
MalI GATC 3 cut(s) 105, 1052, 1191
MbiI CCGCTC 1 cut(s) 706
MboI GATC 3 cut(s) 103, 1050, 1189
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 951
MlsI TGGCCA 1 cut(s) 971
MluCI AATT 3 cut(s) 389, 606, 888
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 467, 1038
Mox20I TGGCCA 1 cut(s) 971
MscI TGGCCA 1 cut(s) 971
MseI TTAA 3 cut(s) 131, 498, 1182
MslI CAYNNNNRTG 1 cut(s) 1172
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 552
MspI CCGG 4 cut(s) 238, 275, 534, 1143
MspR9I CCNGG 3 cut(s) 275, 320, 534
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 8 cut(s) 213, 216, 659, 779, 955, 1029, 1106, 1164
NciI CCSGG 2 cut(s) 275, 534
NdeII GATC 3 cut(s) 103, 1050, 1189
NlaIII CATG 2 cut(s) 1057, 1100
NlaIV GGNNCC 2 cut(s) 314, 573
NspI RCATGY 1 cut(s) 1100
PaqCI CACCTGC 1 cut(s) 991
PceI AGGCCT 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 3 cut(s) 863, 1082, 1212
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 6 cut(s) 125, 209, 218, 554, 705, 1157
PleI GAGTC 1 cut(s) 71
PmaCI CACGTG 1 cut(s) 913
PmlI CACGTG 1 cut(s) 913
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 913
Psp1406I AACGTT 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 318
PspCI CACGTG 1 cut(s) 913
PspFI CCCAGC 2 cut(s) 548, 928
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 314, 573
PspPI GGNCC 2 cut(s) 313, 571
PstI CTGCAG 3 cut(s) 902, 946, 1002
PstNI CAGNNNCTG 1 cut(s) 1122
PsuI RGATCY 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 1172
SaqAI TTAA 3 cut(s) 131, 498, 1182
SatI GCNGC 6 cut(s) 124, 208, 217, 553, 704, 1156
Sau3AI GATC 3 cut(s) 103, 1050, 1189
Sau96I GGNCC 2 cut(s) 313, 571
SbfI CCTGCAGG 1 cut(s) 902
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 275, 320, 534
SdaI CCTGCAGG 1 cut(s) 902
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 4 cut(s) 898, 942, 998, 1072
SinI GGWCC 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 1172
SmlI CTYRAG 2 cut(s) 416, 473
SmoI CTYRAG 2 cut(s) 416, 473
Sse8387I CCTGCAGG 1 cut(s) 902
Sse9I AATT 3 cut(s) 389, 606, 888
SseBI AGGCCT 1 cut(s) 400
SspMI CTAG 1 cut(s) 407
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 3 cut(s) 273, 318, 532
TaaI ACNGT 5 cut(s) 309, 436, 517, 634, 982
TaiI ACGT 3 cut(s) 162, 449, 915
TaqI TCGA 2 cut(s) 1161, 1210
TaqII GACCGA 1 cut(s) 604
TasI AATT 3 cut(s) 389, 606, 888
TauI GCSGC 4 cut(s) 210, 219, 555, 706
TfiI GAWTC 3 cut(s) 863, 1082, 1212
Tru1I TTAA 3 cut(s) 131, 498, 1182
Tru9I TTAA 3 cut(s) 131, 498, 1182
TscAI CASTG 6 cut(s) 115, 637, 951, 985, 1174, 1211
TseI GCWGC 2 cut(s) 123, 1155
TspDTI ATGAA 2 cut(s) 20, 1204
TspRI CASTG 6 cut(s) 115, 637, 951, 985, 1174, 1211
VneI GTGCAC 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 571
XapI RAATTY 2 cut(s) 389, 606
XceI RCATGY 1 cut(s) 1100
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.