RchiOBHm_Chr2g0107721

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009502
Physical Location & Seq
Reverse (-)
19257858 .. 19260044
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48173

Sequence Viewer

Length: 885 bp
ATGGTGAAGATGGTGTGGCTTATCTTCTTCCAGCTTCTGTTTCTGGGTCTTTCTGGTGCAAGGCAAGAATCAGCTCAAGTCCTAACTCTCTCTGAGACATCCCATGAGGTTCTACAAGATTCAACCTACTCTGTGAACCCACCTCCAAAGGTCTCACCTTTCTCTGATGAAACCCTTAAACTGGTGTCTTCTCACTCAGAAACCCTTAAAAAGCTTGGATTTTTGGGGCTCAGGAACATCAATGTTATAGTCACCAACCCAAAGGAGGCAAAACCAAGAACCAGAAAGCTCTCTTCCATTGACCCATTTCCAGCTAGACCAAACCCACTACCCAAAACCTCAGACCCCCCACTCCACTCCTCTGTTGGCTTCTCTGTTCCTGCCAATGTAGCCAAAAACCCTCAACCTCCACAAGCTCAAGTAGCCTCACCAGCACCAGCCCAAACAGCCTCACCTCCAATCCCTGAAACAGCATCACCCCCAGTCGAAATGTCTTTTCCCGAAGCCCCCGAAATCTCTCCTGTTTACCATCAAGCCACCCCTCCCCCTTTTGCTTACGAATTGCCACCGTGTAATCCGAGAGCCCCGGCACCTGGAATTGGGTTTGTGCATAAACTGTGGTGTGTAGCCAAGCCTACTGTTCCGGCAGACACATTGCAGGAGGCTGTCGACTATGCTTGTGGAGCTGGTGGTGCTGATTGCCAGGAGATTATGCCAAGCGGAAACTGCTACTATCCTGAGTCTGTTGTGGCCCATGCTTCGTATGCTTTCAACAGCTACTGGCAGAAGAACAAGAGGAATGGAGGCACTTGCAGCTTTGGTGGCACTGCTATGCTTATCAACAGTGATCCAAGTTATCAACTATGCCGTTTTGTTATCACCTAA

Protein Analysis

294

Amino Acids

31.68

Weight (kDa)

6.99

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 206 - 278 1.4e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 669
AciI CCGC 1 cut(s) 720
AclWI GGATC 1 cut(s) 842
AfiI CCNNNNNNNGG 4 cut(s) 181, 265, 593, 599
AgsI TTSAA 2 cut(s) 123, 772
AjnI CCWGG 2 cut(s) 592, 702
AluBI AGCT 9 cut(s) 34, 74, 214, 289, 314, 416, 686, 777, 816
AluI AGCT 9 cut(s) 34, 74, 214, 289, 314, 416, 686, 777, 816
Alw26I GTCTC 2 cut(s) 89, 157
AlwI GGATC 1 cut(s) 842
AlwNI CAGNNNCTG 2 cut(s) 37, 780
AoxI GGCC 1 cut(s) 750
ApeKI GCWGC 1 cut(s) 813
AspS9I GGNCC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 587
AsuHPI GGTGA 7 cut(s) 16, 147, 244, 420, 444, 468, 871
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 231, 586
BbsI GAAGAC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 825
BccI CCATC 2 cut(s) 4, 537
BceAI ACGGC 1 cut(s) 852
BciT130I CCWGG 2 cut(s) 594, 704
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 2 cut(s) 89, 157
BfaI CTAG 1 cut(s) 315
BisI GCNGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 815
Bme1390I CCNGG 3 cut(s) 587, 594, 704
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 3 cut(s) 587, 594, 704
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 1 cut(s) 482
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 230
BpuEI CTTGAG 2 cut(s) 60, 402
BpuMI CCSGG 1 cut(s) 587
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 585
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 265, 593, 599
Bse1I ACTGG 3 cut(s) 186, 482, 785
Bse3DI GCAATG 1 cut(s) 653
BseBI CCWGG 2 cut(s) 594, 704
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 4 cut(s) 181, 265, 593, 599
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 5 cut(s) 84, 210, 244, 354, 729
BseNI ACTGG 3 cut(s) 186, 482, 785
BseRI GAGGAG 1 cut(s) 349
BseXI GCAGC 1 cut(s) 825
BshFI GGCC 1 cut(s) 752
BshNI GGYRCC 1 cut(s) 589
BsiSI CCGG 2 cut(s) 587, 644
BslI CCNNNNNNNGG 4 cut(s) 181, 265, 593, 599
BsmAI GTCTC 2 cut(s) 89, 157
BsnI GGCC 1 cut(s) 752
Bso31I GGTCTC 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 231, 586
Bsp143I GATC 1 cut(s) 847
BspACI CCGC 1 cut(s) 720
BspANI GGCC 1 cut(s) 752
BspCNI CTCAG 5 cut(s) 85, 209, 243, 353, 730
BspLI GGNNCC 1 cut(s) 591
BspPI GGATC 1 cut(s) 842
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 653
BsrI ACTGG 3 cut(s) 186, 482, 785
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 1 cut(s) 847
Bst2UI CCWGG 2 cut(s) 594, 704
Bst4CI ACNGT 4 cut(s) 570, 618, 640, 845
Bst6I CTCTTC 1 cut(s) 298
BstDEI CTNAG 5 cut(s) 93, 196, 230, 340, 738
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 1 cut(s) 850
BstMAI GTCTC 2 cut(s) 89, 157
BstMBI GATC 1 cut(s) 847
BstNI CCWGG 2 cut(s) 594, 704
BstSCI CCNGG 3 cut(s) 585, 592, 702
BstV1I GCAGC 1 cut(s) 825
BstV2I GAAGAC 1 cut(s) 180
BsuRI GGCC 1 cut(s) 752
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 2 cut(s) 825, 850
CaiI CAGNNNCTG 2 cut(s) 37, 780
Cfr13I GGNCC 1 cut(s) 751
CviAII CATG 2 cut(s) 104, 755
DdeI CTNAG 5 cut(s) 93, 196, 230, 340, 738
DpnI GATC 1 cut(s) 849
DpnII GATC 1 cut(s) 847
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Eco24I GRGCYC 2 cut(s) 231, 586
Eco31I GGTCTC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 592, 702
EcoT38I GRGCYC 2 cut(s) 231, 586
FaeI CATG 2 cut(s) 107, 758
FaiI YATR 9 cut(s) 105, 248, 612, 675, 713, 756, 765, 833, 865
FatI CATG 2 cut(s) 103, 754
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 1 cut(s) 814
FokI GGATG 1 cut(s) 85
FriOI GRGCYC 2 cut(s) 231, 586
Fsp4HI GCNGC 1 cut(s) 814
FspBI CTAG 1 cut(s) 315
GluI GCNGC 1 cut(s) 814
HaeIII GGCC 1 cut(s) 752
HapII CCGG 2 cut(s) 587, 644
Hin1II CATG 2 cut(s) 107, 758
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 3 cut(s) 68, 119, 740
HpaII CCGG 2 cut(s) 587, 644
HphI GGTGA 7 cut(s) 16, 147, 244, 420, 444, 468, 871
Hpy166II GTNNAC 3 cut(s) 136, 526, 670
Hpy188I TCNGA 5 cut(s) 94, 166, 199, 343, 579
Hpy188III TCNNGA 3 cut(s) 232, 500, 737
Hpy8I GTNNAC 3 cut(s) 136, 526, 670
HpyCH4III ACNGT 4 cut(s) 570, 618, 640, 845
HpyCH4V TGCA 4 cut(s) 59, 610, 658, 813
HpyF3I CTNAG 5 cut(s) 93, 196, 230, 340, 738
Hsp92II CATG 2 cut(s) 107, 758
Kzo9I GATC 1 cut(s) 847
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 1 cut(s) 825
LweI GCATC 1 cut(s) 482
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 250
MalI GATC 1 cut(s) 849
MboI GATC 1 cut(s) 847
MboII GAAGA 6 cut(s) 16, 19, 19, 180, 285, 799
MhlI GDGCHC 2 cut(s) 231, 586
MluCI AATT 2 cut(s) 560, 597
MlyI GAGTC 1 cut(s) 749
MseI TTAA 2 cut(s) 177, 207
MslI CAYNNNNRTG 1 cut(s) 830
MspI CCGG 2 cut(s) 587, 644
MspR9I CCNGG 3 cut(s) 587, 594, 704
MvaI CCWGG 2 cut(s) 594, 704
NciI CCSGG 1 cut(s) 587
NdeII GATC 1 cut(s) 847
NlaIII CATG 2 cut(s) 107, 758
NlaIV GGNNCC 1 cut(s) 591
NmuCI GTSAC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 68, 119
PkrI GCNGC 1 cut(s) 815
PleI GAGTC 1 cut(s) 748
PpsI GAGTC 1 cut(s) 748
Psp6I CCWGG 2 cut(s) 592, 702
PspGI CCWGG 2 cut(s) 592, 702
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 751
PstNI CAGNNNCTG 2 cut(s) 37, 780
RseI CAYNNNNRTG 1 cut(s) 830
SalI GTCGAC 1 cut(s) 668
SaqAI TTAA 2 cut(s) 177, 207
SatI GCNGC 1 cut(s) 814
Sau3AI GATC 1 cut(s) 847
Sau96I GGNCC 1 cut(s) 751
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 3 cut(s) 587, 594, 704
SduI GDGCHC 2 cut(s) 231, 586
SfaNI GCATC 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 2 cut(s) 75, 417
SmoI CTYRAG 2 cut(s) 75, 417
Sse9I AATT 2 cut(s) 560, 597
SsiI CCGC 1 cut(s) 720
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 3 cut(s) 585, 592, 702
TaaI ACNGT 4 cut(s) 570, 618, 640, 845
TaqI TCGA 2 cut(s) 486, 669
TasI AATT 2 cut(s) 560, 597
TfiI GAWTC 2 cut(s) 68, 119
Tru1I TTAA 2 cut(s) 177, 207
Tru9I TTAA 2 cut(s) 177, 207
TscAI CASTG 2 cut(s) 832, 850
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 813
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 1 cut(s) 183
TspRI CASTG 2 cut(s) 832, 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.