MD15G1295500.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009502
Physical Location & Seq
Reverse (-)
27713304 .. 27715120
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1295500.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGTTGAAAAAGATGGTGTTCTTCTTTCATCTTCTTTTTCTCCTTTCGTTTTTTGTTTCTGGTGAAAGGCAAGACTCCATTGTTTCTGTAAAATCTGGAGATCTCAGTGAGGTGGCAAGCAGCATTTTGAAGGCAGAAACTTGGCTTAAAACCCACGTCCTCGCTCACTACCCTTCCATCTCCATCACCGCCATTGTCGTCGGCGGTGGTGGTGGCCCTCTTCTCTGCCGGAAAGAGCAAGAAAACACTCTCGCTCTCGTCCTCCCAGCCCTCAAGAACTTGCACCACTCCCTCACACGGTGGGGCCTCCAGGAACACATCAAACTCTCAGCCACCTTCGCTCCTTCTTGTTTCGACAGAAGCTCCTATATTTTTGGGAAATTTATTAGGCCTGTCCTAGATTTTATCAAGGGCGCATCTCCAACGTTTGTGTCTTCTCACTCCGAAAGCCTTGAAGATTTTATCGGCGTTAGCTTTAACTCCGACGATGTTCCTGTCGTTCTCGACAACCCGGGAGAGAGAAAACCCAGCGGCAGAAAGCTCTCTGCCGACCCCTTTCCGGCGAGACCGACTCCGTTGCCTGAAATCTCGGAAACCCCGCTTCACTCTTCCGTTGGTTTCTCAGTCCCCGCCAATGTGGCTAAAAACCCTCATCCTCCACAGCCCCAAACGGCCTCTCCGCCACTCCAAGTGCCTTCCCCACAACCCCGAACCGCCTTTCCTCCGCCGCCACTAATGCCTTCCCCGGAGGCACAAGCAGCCTCACCACCACCACCGCAAATAGCCTCTCCACCAGATATGTCTTTCTCCTCAGCCCCCGAAAACCCTCCTTTTGTTGATGAATCCCCTCCTCCTCCGCCTTCCCCTGCCACATTTCCACCCTGCCGCAATCCGATTCCGACACATGTGCCGCCTCCTGCCCTGGCCCCTCAGATGGCGGTGCACAAGCTGTGGTGTGTGGCCAAGCCTACCGTTCCTTCAGACACTCTGCAGGTGGCAATGGACTTTGCCTGCGGAGACGGCAATGCTGACTGCAAGGAGATCATGCCAGATGGCAACTGCTACAACCCTGATTCTGTTGTGGCACATGCTTCCTATGCCTTCAACAGCTACTGGCAGAAGAACAAGAGGACCGGAGGGACTTGCAGCTTCGATGGCACTGCAATGCTGATCAACAATGATCCAAGTTATCTTCAATGTCGCTTCGTTCTCACCTGA

Protein Analysis

406

Amino Acids

43.68

Weight (kDa)

6.3

Isoelectric Point (pI)

66.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 317 - 389 2.6e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 982
Acc36I ACCTGC 1 cut(s) 982
AclI AACGTT 1 cut(s) 425
AclWI GGATC 1 cut(s) 1175
AcoI YGGCCR 1 cut(s) 960
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 1 cut(s) 963
AdeI CACNNNGTG 1 cut(s) 300
AfiI CCNNNNNNNGG 3 cut(s) 297, 559, 934
AflIII ACRYGT 1 cut(s) 904
AgsI TTSAA 5 cut(s) 7, 130, 455, 1105, 1196
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 2 cut(s) 309, 921
AloI GAACNNNNNNTCC 2 cut(s) 703, 735
AluBI AGCT 6 cut(s) 363, 474, 541, 949, 1110, 1149
AluI AGCT 6 cut(s) 363, 474, 541, 949, 1110, 1149
Alw21I GWGCWC 1 cut(s) 945
Alw26I GTCTC 2 cut(s) 559, 1011
Alw44I GTGCAC 1 cut(s) 941
AlwI GGATC 1 cut(s) 1175
AlwNI CAGNNNCTG 1 cut(s) 1113
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 6 cut(s) 214, 304, 389, 672, 924, 960
ApaLI GTGCAC 1 cut(s) 941
ApeKI GCWGC 3 cut(s) 120, 758, 1146
ApoI RAATTY 1 cut(s) 380
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 4 cut(s) 215, 304, 925, 1131
AsuC2I CCSGG 3 cut(s) 512, 513, 746
AsuHPI GGTGA 4 cut(s) 74, 178, 756, 1204
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 1 cut(s) 1131
BaeGI GKGCMC 1 cut(s) 945
BalI TGGCCA 1 cut(s) 962
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 945
BbvCI CCTCAGC 1 cut(s) 811
BbvI GCAGC 3 cut(s) 132, 770, 1158
BccI CCATC 6 cut(s) 7, 185, 191, 928, 1046, 1148
BceAI ACGGC 2 cut(s) 687, 1036
BcgI CGANNNNNNTGC 6 cut(s) 559, 593, 889, 923, 1142, 1176
BciT130I CCWGG 2 cut(s) 311, 923
BclI TGATCA 1 cut(s) 1170
BcnI CCSGG 3 cut(s) 512, 513, 746
BcoDI GTCTC 2 cut(s) 559, 1011
BfaI CTAG 1 cut(s) 398
BfmI CTRYAG 1 cut(s) 989
BfuAI ACCTGC 1 cut(s) 982
BglI GCCNNNNNGGC 1 cut(s) 638
BglII AGATCT 1 cut(s) 100
BisI GCNGC 7 cut(s) 121, 532, 728, 759, 886, 911, 1147
BlsI GCNGC 7 cut(s) 122, 533, 729, 760, 887, 912, 1148
Bme1390I CCNGG 5 cut(s) 311, 512, 513, 746, 923
Bme18I GGWCC 1 cut(s) 1131
BmeT110I CYCGRG 1 cut(s) 511
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 4 cut(s) 215, 304, 925, 1131
BmiI GGNNCC 2 cut(s) 305, 927
BmrFI CCNGG 5 cut(s) 311, 512, 513, 746, 923
BmsI GCATC 1 cut(s) 425
BpiI GAAGAC 1 cut(s) 426
BplI GAGNNNNNCTC 2 cut(s) 556, 588
BpmI CTGGAG 2 cut(s) 117, 293
Bpu10I CCTNAGC 1 cut(s) 811
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 3 cut(s) 512, 513, 746
BsaI GGTCTC 1 cut(s) 559
BsaJI CCNNGG 3 cut(s) 511, 744, 921
BsaWI WCCGGW 1 cut(s) 1133
BsaXI ACNNNNNCTCC 6 cut(s) 325, 347, 355, 377, 661, 691
Bsc4I CCNNNNNNNGG 3 cut(s) 297, 559, 934
Bse1I ACTGG 1 cut(s) 1118
Bse3DI GCAATG 3 cut(s) 1005, 1030, 1170
BseBI CCWGG 2 cut(s) 311, 923
BseDI CCNNGG 3 cut(s) 511, 744, 921
BseGI GGATG 1 cut(s) 652
BseLI CCNNNNNNNGG 3 cut(s) 297, 559, 934
BseMI GCAATG 3 cut(s) 1005, 1030, 1170
BseMII CTCAG 5 cut(s) 118, 342, 636, 825, 944
BseNI ACTGG 1 cut(s) 1118
BseRI GAGGAG 3 cut(s) 799, 840, 843
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 3 cut(s) 132, 770, 1158
BseYI CCCAGC 2 cut(s) 265, 527
BshFI GGCC 6 cut(s) 216, 306, 391, 674, 926, 962
BsiHKAI GWGCWC 1 cut(s) 945
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 5 cut(s) 229, 512, 560, 746, 1134
BslFI GGGAC 2 cut(s) 611, 1153
BslI CCNNNNNNNGG 3 cut(s) 297, 559, 934
BsmAI GTCTC 2 cut(s) 559, 1011
BsmBI CGTCTC 1 cut(s) 1011
BsmFI GGGAC 2 cut(s) 611, 1153
BsnI GGCC 6 cut(s) 216, 306, 391, 674, 926, 962
Bso31I GGTCTC 1 cut(s) 559
BsoBI CYCGRG 1 cut(s) 511
Bsp1286I GDGCHC 1 cut(s) 945
Bsp143I GATC 4 cut(s) 100, 1041, 1170, 1180
BspANI GGCC 6 cut(s) 216, 306, 391, 674, 926, 962
BspCNI CTCAG 5 cut(s) 117, 341, 635, 824, 943
BspLI GGNNCC 2 cut(s) 305, 927
BspMAI CTGCAG 1 cut(s) 993
BspMI ACCTGC 1 cut(s) 982
BspPI GGATC 1 cut(s) 1175
BspTNI GGTCTC 1 cut(s) 559
BsrDI GCAATG 3 cut(s) 1005, 1030, 1170
BsrI ACTGG 1 cut(s) 1118
BssECI CCNNGG 3 cut(s) 511, 744, 921
BssMI GATC 4 cut(s) 100, 1041, 1170, 1180
Bst2UI CCWGG 2 cut(s) 311, 923
Bst4CI ACNGT 2 cut(s) 300, 973
Bst6I CTCTTC 2 cut(s) 225, 613
BstC8I GCNNGC 2 cut(s) 118, 1012
BstDEI CTNAG 5 cut(s) 104, 328, 622, 811, 930
BstF5I GGATG 1 cut(s) 652
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 4 cut(s) 103, 1044, 1173, 1183
BstMAI GTCTC 2 cut(s) 559, 1011
BstMBI GATC 4 cut(s) 100, 1041, 1170, 1180
BstMWI GCNNNNNNNGC 7 cut(s) 338, 638, 736, 758, 1020, 1097, 1155
BstNI CCWGG 2 cut(s) 311, 923
BstNSI RCATGY 2 cut(s) 908, 1091
BstSCI CCNGG 5 cut(s) 309, 510, 511, 744, 921
BstSFI CTRYAG 1 cut(s) 989
BstSLI GKGCMC 1 cut(s) 945
BstV1I GCAGC 3 cut(s) 132, 770, 1158
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 6 cut(s) 216, 306, 391, 674, 926, 962
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 1 cut(s) 652
BtsI GCAGTG 1 cut(s) 1158
BtsIMutI CAGTG 2 cut(s) 112, 1158
BveI ACCTGC 1 cut(s) 982
Cac8I GCNNGC 2 cut(s) 118, 1012
CaiI CAGNNNCTG 1 cut(s) 1113
CfoI GCGC 1 cut(s) 416
Cfr13I GGNCC 4 cut(s) 215, 304, 925, 1131
Cfr9I CCCGGG 1 cut(s) 511
CviAII CATG 3 cut(s) 905, 1045, 1088
DdeI CTNAG 5 cut(s) 104, 328, 622, 811, 930
DpnI GATC 4 cut(s) 102, 1043, 1172, 1182
DpnII GATC 4 cut(s) 100, 1041, 1170, 1180
DraIII CACNNNGTG 1 cut(s) 300
EaeI YGGCCR 1 cut(s) 960
Eam1104I CTCTTC 2 cut(s) 225, 613
EarI CTCTTC 2 cut(s) 225, 613
EciI GGCGGA 3 cut(s) 669, 714, 846
Eco147I AGGCCT 1 cut(s) 391
Eco31I GGTCTC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 1131
Eco57I CTGAAG 1 cut(s) 963
Eco88I CYCGRG 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 309, 921
Esp3I CGTCTC 1 cut(s) 1011
FaeI CATG 3 cut(s) 908, 1048, 1091
FaiI YATR 6 cut(s) 369, 800, 906, 1046, 1089, 1098
FaqI GGGAC 2 cut(s) 611, 1153
FatI CATG 3 cut(s) 904, 1044, 1087
FauI CCCGC 2 cut(s) 606, 637
FbaI TGATCA 1 cut(s) 1170
Fnu4HI GCNGC 7 cut(s) 121, 532, 728, 759, 886, 911, 1147
FokI GGATG 1 cut(s) 639
Fsp4HI GCNGC 7 cut(s) 121, 532, 728, 759, 886, 911, 1147
FspBI CTAG 1 cut(s) 398
GlaI GCGC 1 cut(s) 415
GluI GCNGC 7 cut(s) 121, 532, 728, 759, 886, 911, 1147
GsaI CCCAGC 2 cut(s) 269, 531
GsuI CTGGAG 2 cut(s) 117, 293
HaeIII GGCC 6 cut(s) 216, 306, 391, 674, 926, 962
HapII CCGG 5 cut(s) 229, 512, 560, 746, 1134
HhaI GCGC 1 cut(s) 416
Hin1II CATG 3 cut(s) 908, 1048, 1091
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HinfI GANTC 5 cut(s) 74, 571, 842, 895, 1073
HpaII CCGG 5 cut(s) 229, 512, 560, 746, 1134
HphI GGTGA 4 cut(s) 74, 178, 756, 1204
Hpy166II GTNNAC 1 cut(s) 943
Hpy188I TCNGA 7 cut(s) 445, 484, 592, 894, 900, 933, 982
Hpy188III TCNNGA 3 cut(s) 96, 274, 503
Hpy8I GTNNAC 1 cut(s) 943
Hpy99I CGWCG 2 cut(s) 203, 488
HpyAV CCTTC 9 cut(s) 124, 183, 346, 354, 705, 750, 870, 987, 1111
HpyCH4III ACNGT 2 cut(s) 300, 973
HpyCH4IV ACGT 2 cut(s) 156, 425
HpyCH4V TGCA 6 cut(s) 283, 943, 991, 1035, 1146, 1163
HpyF10VI GCNNNNNNNGC 7 cut(s) 338, 638, 736, 758, 1020, 1097, 1155
HpyF3I CTNAG 5 cut(s) 104, 328, 622, 811, 930
HpySE526I ACGT 2 cut(s) 156, 425
Hsp92II CATG 3 cut(s) 908, 1048, 1091
HspAI GCGC 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 1170
Kzo9I GATC 4 cut(s) 100, 1041, 1170, 1180
LmnI GCTCC 2 cut(s) 346, 368
Lsp1109I GCAGC 3 cut(s) 132, 770, 1158
LweI GCATC 1 cut(s) 425
MaeI CTAG 1 cut(s) 398
MaeII ACGT 2 cut(s) 156, 425
MalI GATC 4 cut(s) 102, 1043, 1172, 1182
MboI GATC 4 cut(s) 100, 1041, 1170, 1180
MboII GAAGA 8 cut(s) 13, 23, 212, 426, 467, 600, 1132, 1184
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 945
MlsI TGGCCA 1 cut(s) 962
MluCI AATT 1 cut(s) 380
MluNI TGGCCA 1 cut(s) 962
MlyI GAGTC 2 cut(s) 68, 565
MmeI TCCRAC 3 cut(s) 446, 507, 923
Mox20I TGGCCA 1 cut(s) 962
MscI TGGCCA 1 cut(s) 962
MseI TTAA 2 cut(s) 147, 477
MslI CAYNNNNRTG 1 cut(s) 1163
Msp20I TGGCCA 1 cut(s) 962
MspA1I CMGCKG 1 cut(s) 531
MspI CCGG 5 cut(s) 229, 512, 560, 746, 1134
MspR9I CCNGG 5 cut(s) 311, 512, 513, 746, 923
MvaI CCWGG 2 cut(s) 311, 923
MwoI GCNNNNNNNGC 7 cut(s) 338, 638, 736, 758, 1020, 1097, 1155
NciI CCSGG 3 cut(s) 512, 513, 746
NdeII GATC 4 cut(s) 100, 1041, 1170, 1180
NlaIII CATG 3 cut(s) 908, 1048, 1091
NlaIV GGNNCC 2 cut(s) 305, 927
NspI RCATGY 2 cut(s) 908, 1091
PaqCI CACCTGC 1 cut(s) 982
PceI AGGCCT 1 cut(s) 391
PciI ACATGT 1 cut(s) 904
PfeI GAWTC 3 cut(s) 842, 895, 1073
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 7 cut(s) 122, 533, 729, 760, 887, 912, 1148
PleI GAGTC 2 cut(s) 68, 565
PpsI GAGTC 2 cut(s) 68, 565
PscI ACATGT 1 cut(s) 904
Psp1406I AACGTT 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 309, 921
PspFI CCCAGC 2 cut(s) 265, 527
PspGI CCWGG 2 cut(s) 309, 921
PspN4I GGNNCC 2 cut(s) 305, 927
PspPI GGNCC 4 cut(s) 215, 304, 925, 1131
PstI CTGCAG 1 cut(s) 993
PstNI CAGNNNCTG 1 cut(s) 1113
PsuI RGATCY 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 1163
SaqAI TTAA 2 cut(s) 147, 477
SatI GCNGC 7 cut(s) 121, 532, 728, 759, 886, 911, 1147
Sau3AI GATC 4 cut(s) 100, 1041, 1170, 1180
Sau96I GGNCC 4 cut(s) 215, 304, 925, 1131
SchI GAGTC 2 cut(s) 68, 565
ScrFI CCNGG 5 cut(s) 311, 512, 513, 746, 923
SduI GDGCHC 1 cut(s) 945
SfaNI GCATC 1 cut(s) 425
SfcI CTRYAG 1 cut(s) 989
SinI GGWCC 1 cut(s) 1131
SmaI CCCGGG 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 1163
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 380
SseBI AGGCCT 1 cut(s) 391
SspMI CTAG 1 cut(s) 398
StuI AGGCCT 1 cut(s) 391
StyD4I CCNGG 5 cut(s) 309, 510, 511, 744, 921
TaaI ACNGT 2 cut(s) 300, 973
TaiI ACGT 2 cut(s) 159, 428
TaqI TCGA 3 cut(s) 354, 504, 1152
TaqII GACCGA 1 cut(s) 583
TasI AATT 1 cut(s) 380
TauI GCSGC 4 cut(s) 534, 730, 888, 913
TfiI GAWTC 3 cut(s) 842, 895, 1073
Tru1I TTAA 2 cut(s) 147, 477
Tru9I TTAA 2 cut(s) 147, 477
TscAI CASTG 2 cut(s) 112, 1165
TseI GCWGC 3 cut(s) 120, 758, 1146
TspDTI ATGAA 2 cut(s) 17, 855
TspGWI ACGGA 2 cut(s) 564, 601
TspMI CCCGGG 1 cut(s) 511
TspRI CASTG 2 cut(s) 112, 1165
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 1131
XapI RAATTY 1 cut(s) 380
XceI RCATGY 2 cut(s) 908, 1091
XmaI CCCGGG 1 cut(s) 511
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.