MD02G1192600.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014190
Physical Location & Seq
Forward (+)
18226664 .. 18228431
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1192600.v1.1.491

Sequence Viewer

Length: 885 bp
ATGGCCCTTTACCCTAGTTGGAGTTGGAGGGCTGTCGTTGCTTCTTTCTGCTTTTGCTTTCTTTCTTTATTTTCTCTTTCATCTTTTGGACGACCAGCCACTTTTCTTCAGGACTTCCGAGTCACATGGTCTGATTCTCATATCAGGCAAATCGATGGAGGGAGGGCCATCCAACTCGTTCTTGACCAAAATTCTGGATGTGGGTTTGCCTCCAAACAGAAGTACTTGTTTGGGCGTGTGAGCATGAAGATCAAGCTCATCCCTGGAGACTCTGCTGGAACTGTCACTGCTTTTTATATGAACTCTGACACCGATACAGTTCGTGACGAGCTAGATTTTGAATTCTTGGGGAACCGGACTGGACAGCCTTATACAGTCCAAACCAATATCTATGCTCATGGGAAGGGTGATAGGGAGCAAAGGGTTAACCTCTGGTTTGACCCTGCTGCTGATTTCCACACTTACACAATCCTCTGGAATCATCACCATATTGTCTTCTATGTGGATGATATGCCTATAAGGGTGTACAAGAACAATGAAGCCAAAGGAGTCCCATACCCAAAGCTCCAACCTATGGGAGTGTTTTCGACGTTGTGGGAAGCTGATGATTGGGCTACTAGAGGAGGACTTGAGAAGATAAACTGGAGCAAAGCTCCTTTCTTTTCATATTACAAGGATTTTGACATTGAGGGATGCAGTGTGCCAGGACCAGCCTCTTGTGCCTCAAGCACCAATAACTGGTGGGAAGGCGCTTCCTACCAAGCCCTCAGCGCCCTCGATTATCGAAGATACAGGTGGGTTCGTATCAACCACATGATCTACGACTACTGCACCGACAGATCCCGGTACCCAGTAGCCCCACCGGAGTGCACCGCCGGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0010035 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

295

Amino Acids

33.77

Weight (kDa)

6.65

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 37 - 217 2.4e-65 Glycosyl hydrolases family 16
XET_C PF06955 243 - 290 2.4e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37800 AT5G65730
fragaria_vesca FvH4_1g18550
malus_domestica MD02G1192600.v1.1 MD15G1303500.v1.1
prunus_persica Prupe.6G213600_v2.0.a1 Prupe.6G213600_v2.0.a1
pyrus_communis pycom02g15680 pycom15g26640
rosa_chinensis RchiOBHm_Chr2g0109241
rosa_laevigata RLG00000017728
rosa_multiflora Rmu_sc0001807.1_g000003
rosa_roxburghii Rroxscaffold_2G00134370
rosa_rugosa Rorug02G0158800
rosa_samantha Rh2AG210200 Rh2CG212600 Rh2DG216400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 1 cut(s) 846
AccB7I CCANNNNNTGG 2 cut(s) 574, 738
AciI CCGC 1 cut(s) 873
AclWI GGATC 1 cut(s) 834
AcsI RAATTY 2 cut(s) 190, 341
AcuI CTGAAG 1 cut(s) 92
AfaI GTAC 3 cut(s) 224, 527, 848
AfiI CCNNNNNNNGG 2 cut(s) 574, 738
AgsI TTSAA 1 cut(s) 341
AjnI CCWGG 2 cut(s) 262, 703
AleI CACNNNNGTG 1 cut(s) 865
AluBI AGCT 5 cut(s) 256, 331, 565, 602, 653
AluI AGCT 5 cut(s) 256, 331, 565, 602, 653
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 1 cut(s) 261
Alw44I GTGCAC 1 cut(s) 868
AlwI GGATC 1 cut(s) 834
AoxI GGCC 2 cut(s) 3, 165
ApaLI GTGCAC 1 cut(s) 868
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 190, 341
Asp718I GGTACC 1 cut(s) 846
AspLEI GCGC 2 cut(s) 752, 773
AspS9I GGNCC 3 cut(s) 4, 165, 707
AsuC2I CCSGG 1 cut(s) 844
AsuHPI GGTGA 2 cut(s) 419, 476
AvaII GGWCC 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 872
BanI GGYRCC 1 cut(s) 846
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 872
BbvCI CCTCAGC 1 cut(s) 767
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 149, 176
BciT130I CCWGG 2 cut(s) 264, 705
BcnI CCSGG 1 cut(s) 844
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 3 cut(s) 15, 332, 618
BfoI RGCGCY 2 cut(s) 753, 774
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 3 cut(s) 264, 705, 844
Bme18I GGWCC 1 cut(s) 707
BmgT120I GGNCC 3 cut(s) 4, 165, 707
BmiI GGNNCC 2 cut(s) 353, 848
BmrFI CCNGG 3 cut(s) 264, 705, 844
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 1 cut(s) 683
BmuI ACTGGG 1 cut(s) 845
BpiI GAAGAC 1 cut(s) 487
BplI GAGNNNNNCTC 2 cut(s) 637, 669
BpmI CTGGAG 2 cut(s) 285, 664
Bpu10I CCTNAGC 1 cut(s) 767
BpuEI CTTGAG 2 cut(s) 650, 709
BpuMI CCSGG 1 cut(s) 844
Bsa29I ATCGAT 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 262
BsaWI WCCGGW 2 cut(s) 354, 862
Bsc4I CCNNNNNNNGG 2 cut(s) 574, 738
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 4 cut(s) 364, 647, 743, 851
BseBI CCWGG 2 cut(s) 264, 705
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 5 cut(s) 168, 203, 258, 511, 698
BseLI CCNNNNNNNGG 2 cut(s) 574, 738
BseMII CTCAG 1 cut(s) 781
BseNI ACTGG 4 cut(s) 364, 647, 743, 851
BseRI GAGGAG 1 cut(s) 636
BseSI GKGCMC 1 cut(s) 872
BseXI GCAGC 1 cut(s) 433
BsgI GTGCAG 1 cut(s) 814
BshFI GGCC 2 cut(s) 5, 167
BshNI GGYRCC 1 cut(s) 846
BshVI ATCGAT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 4 cut(s) 355, 844, 863, 876
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 2 cut(s) 574, 738
BsmAI GTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 2 cut(s) 5, 167
Bsp1286I GDGCHC 1 cut(s) 872
Bsp1407I TGTACA 1 cut(s) 525
Bsp143I GATC 3 cut(s) 249, 816, 839
BspACI CCGC 1 cut(s) 873
BspANI GGCC 2 cut(s) 5, 167
BspCNI CTCAG 1 cut(s) 780
BspDI ATCGAT 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 353, 848
BspPI GGATC 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 846
BsrFI RCCGGY 1 cut(s) 875
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 4 cut(s) 364, 647, 743, 851
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 3 cut(s) 249, 816, 839
Bst2UI CCWGG 2 cut(s) 264, 705
Bst4CI ACNGT 3 cut(s) 283, 319, 376
BstAUI TGTACA 1 cut(s) 525
BstC8I GCNNGC 1 cut(s) 877
BstDEI CTNAG 1 cut(s) 767
BstF5I GGATG 5 cut(s) 168, 203, 258, 511, 698
BstH2I RGCGCY 2 cut(s) 753, 774
BstHHI GCGC 2 cut(s) 752, 773
BstKTI GATC 3 cut(s) 252, 819, 842
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 3 cut(s) 249, 816, 839
BstMWI GCNNNNNNNGC 4 cut(s) 38, 719, 770, 876
BstNI CCWGG 2 cut(s) 264, 705
BstSCI CCNGG 3 cut(s) 262, 703, 842
BstSLI GKGCMC 1 cut(s) 872
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 487
BstX2I RGATCY 1 cut(s) 839
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 839
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 2 cut(s) 5, 167
BsuTUI ATCGAT 1 cut(s) 153
BtsCI GGATG 5 cut(s) 168, 203, 258, 511, 698
BtsI GCAGTG 2 cut(s) 285, 703
BtsIMutI CAGTG 2 cut(s) 285, 703
Cac8I GCNNGC 1 cut(s) 877
CfoI GCGC 2 cut(s) 752, 773
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 3 cut(s) 4, 165, 707
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 3 cut(s) 223, 526, 847
CviAII CATG 4 cut(s) 126, 244, 398, 814
CviQI GTAC 3 cut(s) 223, 526, 847
DdeI CTNAG 1 cut(s) 767
DpnI GATC 3 cut(s) 251, 818, 841
DpnII GATC 3 cut(s) 249, 816, 839
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 262, 703
FaeI CATG 4 cut(s) 129, 247, 401, 817
FaqI GGGAC 1 cut(s) 536
FatI CATG 4 cut(s) 125, 243, 397, 813
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 5 cut(s) 155, 210, 245, 518, 705
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 3 cut(s) 15, 332, 618
GlaI GCGC 2 cut(s) 751, 772
GluI GCNGC 1 cut(s) 447
GsuI CTGGAG 2 cut(s) 285, 664
HaeII RGCGCY 2 cut(s) 753, 774
HaeIII GGCC 2 cut(s) 5, 167
HapII CCGG 4 cut(s) 355, 844, 863, 876
HhaI GCGC 2 cut(s) 752, 773
Hin1II CATG 4 cut(s) 129, 247, 401, 817
Hin6I GCGC 2 cut(s) 750, 771
HinP1I GCGC 2 cut(s) 750, 771
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 5 cut(s) 120, 134, 269, 478, 549
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 4 cut(s) 355, 844, 863, 876
HphI GGTGA 2 cut(s) 419, 476
Hpy166II GTNNAC 3 cut(s) 427, 526, 870
Hpy188I TCNGA 3 cut(s) 119, 133, 307
Hpy188III TCNNGA 5 cut(s) 110, 182, 195, 323, 475
Hpy8I GTNNAC 3 cut(s) 427, 526, 870
Hpy99I CGWCG 1 cut(s) 592
HpyAV CCTTC 2 cut(s) 397, 740
HpyCH4III ACNGT 3 cut(s) 283, 319, 376
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 3 cut(s) 696, 831, 870
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 719, 770, 876
HpyF3I CTNAG 1 cut(s) 767
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 4 cut(s) 129, 247, 401, 817
HspAI GCGC 2 cut(s) 750, 771
KpnI GGTACC 1 cut(s) 850
KroI GCCGGC 1 cut(s) 875
KroNI GCCGGC 1 cut(s) 877
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 249, 816, 839
LmnI GCTCC 4 cut(s) 415, 570, 645, 658
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 683
MaeI CTAG 3 cut(s) 15, 332, 618
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 3 cut(s) 121, 283, 323
MalI GATC 3 cut(s) 251, 818, 841
MboI GATC 3 cut(s) 249, 816, 839
MboII GAAGA 5 cut(s) 98, 259, 487, 646, 798
MflI RGATCY 1 cut(s) 839
MhlI GDGCHC 1 cut(s) 872
MluCI AATT 2 cut(s) 190, 341
MlyI GAGTC 3 cut(s) 129, 263, 558
MmeI TCCRAC 3 cut(s) 5, 196, 592
MroNI GCCGGC 1 cut(s) 875
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 865
MspI CCGG 4 cut(s) 355, 844, 863, 876
MspR9I CCNGG 3 cut(s) 264, 705, 844
MvaI CCWGG 2 cut(s) 264, 705
MwoI GCNNNNNNNGC 4 cut(s) 38, 719, 770, 876
NaeI GCCGGC 1 cut(s) 877
NciI CCSGG 1 cut(s) 844
NdeII GATC 3 cut(s) 249, 816, 839
NgoMIV GCCGGC 1 cut(s) 875
NlaIII CATG 4 cut(s) 129, 247, 401, 817
NlaIV GGNNCC 2 cut(s) 353, 848
NmuCI GTSAC 3 cut(s) 121, 283, 323
OliI CACNNNNGTG 1 cut(s) 865
PdiI GCCGGC 1 cut(s) 877
PfeI GAWTC 2 cut(s) 134, 478
PflMI CCANNNNNTGG 2 cut(s) 574, 738
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 3 cut(s) 128, 263, 557
PpsI GAGTC 3 cut(s) 128, 263, 557
Psp6I CCWGG 2 cut(s) 262, 703
PspGI CCWGG 2 cut(s) 262, 703
PspN4I GGNNCC 2 cut(s) 353, 848
PspPI GGNCC 3 cut(s) 4, 165, 707
PsuI RGATCY 1 cut(s) 839
RsaI GTAC 3 cut(s) 224, 527, 848
RsaNI GTAC 3 cut(s) 223, 526, 847
RseI CAYNNNNRTG 1 cut(s) 865
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 3 cut(s) 249, 816, 839
Sau96I GGNCC 3 cut(s) 4, 165, 707
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 3 cut(s) 129, 263, 558
ScrFI CCNGG 3 cut(s) 264, 705, 844
SduI GDGCHC 1 cut(s) 872
SetI ASST 9 cut(s) 258, 333, 432, 567, 574, 593, 604, 655, 797
SfaNI GCATC 1 cut(s) 683
SinI GGWCC 1 cut(s) 707
SmiMI CAYNNNNRTG 1 cut(s) 865
SmlI CTYRAG 2 cut(s) 629, 724
SmoI CTYRAG 2 cut(s) 629, 724
Sse9I AATT 2 cut(s) 190, 341
SsiI CCGC 1 cut(s) 873
SspMI CTAG 3 cut(s) 15, 332, 618
StyD4I CCNGG 3 cut(s) 262, 703, 842
TaaI ACNGT 3 cut(s) 283, 319, 376
TaiI ACGT 1 cut(s) 593
TaqI TCGA 4 cut(s) 153, 587, 777, 784
TasI AATT 2 cut(s) 190, 341
TatI WGTACW 2 cut(s) 222, 525
TfiI GAWTC 2 cut(s) 134, 478
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 2 cut(s) 292, 703
TseFI GTSAC 3 cut(s) 121, 283, 323
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 3 cut(s) 121, 283, 323
TspDTI ATGAA 5 cut(s) 69, 260, 314, 552, 654
TspRI CASTG 2 cut(s) 292, 703
Van91I CCANNNNNTGG 2 cut(s) 574, 738
VneI GTGCAC 1 cut(s) 868
VpaK11BI GGWCC 1 cut(s) 707
XapI RAATTY 2 cut(s) 190, 341
XspI CTAG 3 cut(s) 15, 332, 618
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.