Rroxscaffold_2G00134370

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014190
Physical Location & Seq
Forward (+)
71859485 .. 71861302
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134370.1

Sequence Viewer

Length: 798 bp
ATGTTTCCCTCTATGAGGAGTAGTGTTACTGCTTCTATTTCCCTTTGTTTCCTTTCACTATTTTCCCTCTCAGCCTTTGCACGACCAGCCACTTTTCTTCAGGACTTCCAAGTCACATGGTCTGATTCTCATATCAGGCAAATCGATGGAGGGAGGGCCATCCAACTCGTTCTTGACCAAAACTCTGGTTGTGGCTTTTCCTCCAAACACAAGTACTTGTTCGGCCGTGTGAGCATGAAGATCAAGCTCATTCCCGGTGACTCTGCCGGAACTGTCACCGCTTTCTATATGAACTCGGACACAGATACAGTACGTGATGAGCTTGATTTTGAGTTCTTGGGGAACAGGACTGGACAGCCTTACACGGTCCAGACCAATATCTATGCTCATGGACAGGGTAACAGGGAGCAAAGGGTGAACCTCTGGTTTGACCCTGCTGCAGATTTCCACACTTACACAATTCTCTGGAACCATCACCATATTGTCTTCTATGTAGATGATGTGCCTATCAGGCTGTACAAGAACAATGAAGCTAAAGGAATCCCATACCCAAAGCTCCAACCCATGGGGGTGTTCTCAACATTGTGGGAAGCTGATGATTGGGCAACAAGAGGAGGGCTAGAGAAGATAAACTGGAGCAAAGCCCCTTCTATGCTTATTACAAGGACTTTGATATCGAAGGATGCTCTGTGCCAGGACCAGCTAATTGTGCCTCGAGCACTAATAACTGGTGGGAAGGCACTGCTTACCAAGCACTCAATGCCCTTGAATATAGAAGATACAAGTGGGTTCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0010035 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

265

Amino Acids

29.83

Weight (kDa)

7.09

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 34 - 214 2.1e-65 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37800 AT5G65730
fragaria_vesca FvH4_1g18550
malus_domestica MD02G1192600.v1.1 MD15G1303500.v1.1
prunus_persica Prupe.6G213600_v2.0.a1 Prupe.6G213600_v2.0.a1
pyrus_communis pycom02g15680 pycom15g26640
rosa_chinensis RchiOBHm_Chr2g0109241
rosa_laevigata RLG00000017728
rosa_multiflora Rmu_sc0001807.1_g000003
rosa_roxburghii Rroxscaffold_2G00134370
rosa_rugosa Rorug02G0158800
rosa_samantha Rh2AG210200 Rh2CG212600 Rh2DG216400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 565
AciI CCGC 1 cut(s) 279
AcoI YGGCCR 1 cut(s) 223
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 3 cut(s) 215, 312, 518
AfiI CCNNNNNNNGG 2 cut(s) 15, 565
AgsI TTSAA 1 cut(s) 769
AjnI CCWGG 1 cut(s) 693
AluBI AGCT 6 cut(s) 247, 322, 533, 556, 593, 703
AluI AGCT 6 cut(s) 247, 322, 533, 556, 593, 703
Alw21I GWGCWC 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 714
AoxI GGCC 2 cut(s) 156, 223
ApeKI GCWGC 1 cut(s) 437
AspS9I GGNCC 3 cut(s) 156, 367, 697
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 4 cut(s) 268, 269, 427, 467
AvaI CYCGRG 1 cut(s) 714
AvaII GGWCC 2 cut(s) 367, 697
BbsI GAAGAC 1 cut(s) 478
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 1 cut(s) 424
BccI CCATC 3 cut(s) 140, 167, 480
BceAI ACGGC 1 cut(s) 210
BciT130I CCWGG 1 cut(s) 695
BcnI CCSGG 1 cut(s) 255
BfaI CTAG 1 cut(s) 620
BfmI CTRYAG 1 cut(s) 438
BglI GCCNNNNNGGC 1 cut(s) 511
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 255, 695
Bme18I GGWCC 2 cut(s) 367, 697
BmeT110I CYCGRG 1 cut(s) 714
BmgT120I GGNCC 3 cut(s) 156, 367, 697
BmiI GGNNCC 1 cut(s) 470
BmrFI CCNGG 2 cut(s) 255, 695
BmsI GCATC 1 cut(s) 673
BpiI GAAGAC 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 655
BpuMI CCSGG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 564
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 565
Bse1I ACTGG 3 cut(s) 355, 638, 733
BseBI CCWGG 1 cut(s) 695
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 564
BseGI GGATG 2 cut(s) 159, 688
BseLI CCNNNNNNNGG 2 cut(s) 15, 565
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 3 cut(s) 355, 638, 733
BseRI GAGGAG 2 cut(s) 31, 627
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 424
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 158, 225
BshVI ATCGAT 1 cut(s) 144
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 721
BsiHKCI CYCGRG 1 cut(s) 714
BsiSI CCGG 2 cut(s) 255, 267
BslI CCNNNNNNNGG 2 cut(s) 15, 565
BsnI GGCC 2 cut(s) 158, 225
BsoBI CYCGRG 1 cut(s) 714
Bsp1286I GDGCHC 1 cut(s) 721
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 1 cut(s) 279
BspANI GGCC 2 cut(s) 158, 225
BspCNI CTCAG 1 cut(s) 83
BspDI ATCGAT 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 470
BspMAI CTGCAG 1 cut(s) 442
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 3 cut(s) 355, 638, 733
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 695
Bst4CI ACNGT 3 cut(s) 274, 310, 367
BstAPI GCANNNNNTGC 1 cut(s) 760
BstAUI TGTACA 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 314
BstDEI CTNAG 1 cut(s) 70
BstDSI CCRYGG 1 cut(s) 564
BstENI CCTNNNNNAGG 1 cut(s) 13
BstF5I GGATG 2 cut(s) 159, 688
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 6 cut(s) 86, 231, 511, 709, 751, 760
BstNI CCWGG 1 cut(s) 695
BstSCI CCNGG 2 cut(s) 253, 693
BstSFI CTRYAG 1 cut(s) 438
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 1 cut(s) 478
BstXI CCANNNNNNTGG 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 2 cut(s) 158, 225
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 564
BtsCI GGATG 2 cut(s) 159, 688
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 1 cut(s) 740
Cfr13I GGNCC 3 cut(s) 156, 367, 697
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 3 cut(s) 214, 311, 517
CviAII CATG 4 cut(s) 117, 235, 389, 565
CviQI GTAC 3 cut(s) 214, 311, 517
DdeI CTNAG 1 cut(s) 70
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 564
Eco32I GATATC 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 367, 697
Eco52I CGGCCG 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 714
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 693
EcoRV GATATC 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 4 cut(s) 120, 238, 392, 568
FatI CATG 4 cut(s) 116, 234, 388, 564
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 2 cut(s) 146, 695
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 1 cut(s) 620
GluI GCNGC 1 cut(s) 438
GsuI CTGGAG 1 cut(s) 655
HaeIII GGCC 2 cut(s) 158, 225
HapII CCGG 2 cut(s) 255, 267
Hin1II CATG 4 cut(s) 120, 238, 392, 568
HinfI GANTC 3 cut(s) 125, 260, 540
HpaII CCGG 2 cut(s) 255, 267
HphI GGTGA 4 cut(s) 268, 269, 427, 467
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 2 cut(s) 124, 298
Hpy188III TCNNGA 4 cut(s) 101, 173, 370, 466
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 3 cut(s) 657, 673, 730
HpyCH4III ACNGT 3 cut(s) 274, 310, 367
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 2 cut(s) 80, 440
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 231, 511, 709, 751, 760
HpyF3I CTNAG 1 cut(s) 70
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 4 cut(s) 120, 238, 392, 568
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 406, 561, 636
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 673
MaeI CTAG 1 cut(s) 620
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 5 cut(s) 25, 112, 257, 274, 398
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MboII GAAGA 5 cut(s) 89, 250, 478, 637, 788
MhlI GDGCHC 1 cut(s) 721
MluCI AATT 2 cut(s) 459, 705
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 2 cut(s) 187, 583
MslI CAYNNNNRTG 1 cut(s) 569
MspI CCGG 2 cut(s) 255, 267
MspR9I CCNGG 2 cut(s) 255, 695
MvaI CCWGG 1 cut(s) 695
MwoI GCNNNNNNNGC 6 cut(s) 86, 231, 511, 709, 751, 760
NciI CCSGG 1 cut(s) 255
NcoI CCATGG 1 cut(s) 564
NdeII GATC 1 cut(s) 240
NlaIII CATG 4 cut(s) 120, 238, 392, 568
NlaIV GGNNCC 1 cut(s) 470
NmuCI GTSAC 3 cut(s) 112, 257, 274
PaeR7I CTCGAG 1 cut(s) 714
PfeI GAWTC 2 cut(s) 125, 540
PflMI CCANNNNNTGG 1 cut(s) 565
PkrI GCNGC 1 cut(s) 439
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 693
PspGI CCWGG 1 cut(s) 693
PspN4I GGNNCC 1 cut(s) 470
PspPI GGNCC 3 cut(s) 156, 367, 697
PspXI VCTCGAGB 1 cut(s) 714
PstI CTGCAG 1 cut(s) 442
RsaI GTAC 3 cut(s) 215, 312, 518
RsaNI GTAC 3 cut(s) 214, 311, 517
RseI CAYNNNNRTG 1 cut(s) 569
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 3 cut(s) 156, 367, 697
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 255, 695
SduI GDGCHC 1 cut(s) 721
SetI ASST 8 cut(s) 249, 316, 324, 423, 535, 558, 595, 705
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 714
SinI GGWCC 2 cut(s) 367, 697
SlaI CTCGAG 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 714
SmoI CTYRAG 1 cut(s) 714
Sse9I AATT 2 cut(s) 459, 705
SsiI CCGC 1 cut(s) 279
SspMI CTAG 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 253, 693
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 3 cut(s) 274, 310, 367
TaiI ACGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 144, 677, 715
TasI AATT 2 cut(s) 459, 705
TatI WGTACW 2 cut(s) 213, 516
TfiI GAWTC 2 cut(s) 125, 540
TscAI CASTG 1 cut(s) 747
TseFI GTSAC 3 cut(s) 112, 257, 274
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 3 cut(s) 112, 257, 274
TspDTI ATGAA 3 cut(s) 251, 305, 543
TspRI CASTG 1 cut(s) 747
Van91I CCANNNNNTGG 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 367, 697
XagI CCTNNNNNAGG 1 cut(s) 13
XhoI CTCGAG 1 cut(s) 714
XspI CTAG 1 cut(s) 620
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.