Rorug02G0158800

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000575
Physical Location & Seq
Reverse (-)
14147073 .. 14150144
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0158800.1

Sequence Viewer

Length: 675 bp
ATGGGGAGGGTGGACTATTTGGCTATGAAGACTGACCCAGTTGCGGCCACCCAGTTGATGAACTCTGATATCAATGAGCTTAAGATTGCGGCCAAGAGACTCGTTAGGGATGTTACTAGGCTCGGTGGTCTTGGTTTTGGGACTACTTTTCTCAAATGGCTTGCCTCCTTTGCTGCCATATATTTGTTGGTATTGGATCGAACAAACTGGAGAACAAATATGCTGACTTCACTCTTAGTTCCTTATATTTTCTTTAGTCTTCCTTGGGGGCTGTTCCACTTACTGAGAGGAGAGGTTGGAAGCTGGATTGCTTTTGTTGCTGTTGTATTAAGGCTTTTCTTCCCAAGACATTTCCCAGATTGGCTGGAGATGCCGGGTTCTCTGATCCTTCTTCTGGCTGTGGCTCCAAGCTTCTTCGCAGAAACCTTGAAGGAGAGCTGGGTTGGCGTCCTGATATGTCTCCTCATTGGATGTTACCTGCTGCAAGAGCACATCCGGTCATCTGGTGGATTCAGAAATTCATTCACACAAAGCCATGGAATATCAAACACTCTCGTTCAAAATTCGGTTTCTGCTTCTGCCCCGAAATTTGAACATGCGAAGACTGGAATATGGAATGGAACCTTGTTTGTGACTGAGGCTCTTCATTTATATCCTCATGAGCACTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0010035 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

224

Amino Acids

25.16

Weight (kDa)

9.2

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 10 - 185 4.9e-85 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AciI CCGC 2 cut(s) 44, 89
AclWI GGATC 2 cut(s) 204, 379
AcoI YGGCCR 2 cut(s) 45, 90
AcsI RAATTY 3 cut(s) 517, 562, 587
AcyI GRCGYC 1 cut(s) 447
AfiI CCNNNNNNNGG 2 cut(s) 43, 394
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 3 cut(s) 430, 560, 593
AluBI AGCT 4 cut(s) 79, 303, 411, 438
AluI AGCT 4 cut(s) 79, 303, 411, 438
Alw21I GWGCWC 2 cut(s) 492, 666
Alw26I GTCTC 2 cut(s) 91, 464
AlwI GGATC 2 cut(s) 204, 379
AoxI GGCC 2 cut(s) 45, 90
ApeKI GCWGC 2 cut(s) 173, 481
ApoI RAATTY 3 cut(s) 517, 562, 587
AsuC2I CCSGG 1 cut(s) 375
BbsI GAAGAC 3 cut(s) 35, 251, 608
Bbv12I GWGCWC 2 cut(s) 492, 666
BbvI GCAGC 2 cut(s) 160, 468
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 91, 464
BfaI CTAG 1 cut(s) 117
BfrI CTTAAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 486
BisI GCNGC 4 cut(s) 45, 90, 174, 482
BlsI GCNGC 4 cut(s) 46, 91, 175, 483
Bme1390I CCNGG 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 405, 622
BmrFI CCNGG 1 cut(s) 375
BmrI ACTGGG 2 cut(s) 32, 46
BmsI GCATC 1 cut(s) 360
BmuI ACTGGG 2 cut(s) 32, 46
BpiI GAAGAC 3 cut(s) 35, 251, 608
BpmI CTGGAG 2 cut(s) 229, 386
BpuMI CCSGG 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 263, 535
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 394
Bse1I ACTGG 4 cut(s) 38, 52, 212, 610
BseDI CCNNGG 2 cut(s) 263, 535
BseGI GGATG 3 cut(s) 115, 476, 492
BseLI CCNNNNNNNGG 2 cut(s) 43, 394
BseMII CTCAG 2 cut(s) 275, 627
BseNI ACTGG 4 cut(s) 38, 52, 212, 610
BseRI GAGGAG 2 cut(s) 303, 452
BseXI GCAGC 2 cut(s) 160, 468
BseYI CCCAGC 1 cut(s) 438
BshFI GGCC 2 cut(s) 47, 92
BsiHKAI GWGCWC 2 cut(s) 492, 666
BsiSI CCGG 2 cut(s) 374, 496
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 2 cut(s) 43, 394
BsmAI GTCTC 2 cut(s) 91, 464
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 2 cut(s) 47, 92
Bsp1286I GDGCHC 2 cut(s) 492, 666
Bsp143I GATC 2 cut(s) 196, 384
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 2 cut(s) 44, 89
BspANI GGCC 2 cut(s) 47, 92
BspCNI CTCAG 2 cut(s) 276, 628
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 405, 622
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 2 cut(s) 204, 379
BspQI GCTCTTC 1 cut(s) 648
BspTI CTTAAG 1 cut(s) 80
BsrI ACTGG 4 cut(s) 38, 52, 212, 610
BssECI CCNNGG 2 cut(s) 263, 535
BssMI GATC 2 cut(s) 196, 384
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 263, 535
Bst6I CTCTTC 1 cut(s) 648
BstACI GRCGYC 1 cut(s) 447
BstAFI CTTAAG 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 3 cut(s) 235, 284, 636
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 3 cut(s) 115, 476, 492
BstKTI GATC 2 cut(s) 199, 387
BstMAI GTCTC 2 cut(s) 91, 464
BstMBI GATC 2 cut(s) 196, 384
BstMWI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 444, 487
BstNSI RCATGY 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 160, 468
BstV2I GAAGAC 3 cut(s) 35, 251, 608
BsuRI GGCC 2 cut(s) 47, 92
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 3 cut(s) 115, 476, 492
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 162
CciI TCATGA 1 cut(s) 658
CseI GACGC 1 cut(s) 436
CviAII CATG 3 cut(s) 536, 596, 659
DdeI CTNAG 3 cut(s) 235, 284, 636
DpnI GATC 2 cut(s) 198, 386
DpnII GATC 2 cut(s) 196, 384
EaeI YGGCCR 2 cut(s) 45, 90
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 263, 535
Eco32I GATATC 1 cut(s) 70
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 2 cut(s) 263, 535
ErhI CCWWGG 2 cut(s) 263, 535
FaeI CATG 3 cut(s) 539, 599, 662
FaqI GGGAC 1 cut(s) 154
FatI CATG 3 cut(s) 535, 595, 658
Fnu4HI GCNGC 4 cut(s) 45, 90, 174, 482
FokI GGATG 3 cut(s) 122, 479, 483
Fsp4HI GCNGC 4 cut(s) 45, 90, 174, 482
FspBI CTAG 1 cut(s) 117
GluI GCNGC 4 cut(s) 45, 90, 174, 482
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 2 cut(s) 229, 386
HaeIII GGCC 2 cut(s) 47, 92
HapII CCGG 2 cut(s) 374, 496
HgaI GACGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 3 cut(s) 539, 599, 662
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 2 cut(s) 99, 510
HpaII CCGG 2 cut(s) 374, 496
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 67, 384, 515
Hpy188III TCNNGA 2 cut(s) 451, 659
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 398, 424
HpyCH4V TGCA 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 444, 487
HpyF3I CTNAG 3 cut(s) 235, 284, 636
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 3 cut(s) 539, 599, 662
Kzo9I GATC 2 cut(s) 196, 384
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 2 cut(s) 160, 468
LweI GCATC 1 cut(s) 360
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 112, 473, 631
MalI GATC 2 cut(s) 198, 386
MboI GATC 2 cut(s) 196, 384
MboII GAAGA 7 cut(s) 40, 251, 331, 383, 406, 613, 635
MhlI GDGCHC 2 cut(s) 492, 666
MluCI AATT 3 cut(s) 517, 562, 587
MlyI GAGTC 1 cut(s) 93
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 6 cut(s) 175, 281, 286, 473, 631, 666
MseI TTAA 2 cut(s) 81, 329
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 2 cut(s) 374, 496
MspR9I CCNGG 1 cut(s) 375
MwoI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 444, 487
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 535
NdeII GATC 2 cut(s) 196, 384
NlaIII CATG 3 cut(s) 539, 599, 662
NlaIV GGNNCC 2 cut(s) 405, 622
NmuCI GTSAC 1 cut(s) 631
NspI RCATGY 1 cut(s) 599
PagI TCATGA 1 cut(s) 658
PciSI GCTCTTC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 510
PkrI GCNGC 4 cut(s) 46, 91, 175, 483
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 405, 622
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 2 cut(s) 81, 329
SatI GCNGC 4 cut(s) 45, 90, 174, 482
Sau3AI GATC 2 cut(s) 196, 384
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 2 cut(s) 492, 666
SetI ASST 8 cut(s) 81, 297, 305, 413, 428, 440, 480, 626
SfaNI GCATC 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 3 cut(s) 517, 562, 587
SsiI CCGC 2 cut(s) 44, 89
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 2 cut(s) 263, 535
TaqI TCGA 1 cut(s) 199
TasI AATT 3 cut(s) 517, 562, 587
TauI GCSGC 2 cut(s) 47, 92
TfiI GAWTC 1 cut(s) 510
Tru1I TTAA 2 cut(s) 81, 329
Tru9I TTAA 2 cut(s) 81, 329
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 2 cut(s) 173, 481
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 4 cut(s) 41, 74, 510, 635
Vha464I CTTAAG 1 cut(s) 80
XapI RAATTY 3 cut(s) 517, 562, 587
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.