Rh6DG156100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000575
Physical Location & Seq
Forward (+)
23442958 .. 23444993
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG156100.1

Sequence Viewer

Length: 411 bp
ATGGACGACGGCCTCAATGAGGACTTCGGTTACGATCCAGATATTTACTACTTTGACACCTCGCTGCAAGCGTCCGATTCATATCATGTGGACATCAATCCAGAAGTTATCTGCAATGATGATGCTGCTTGGGAAAAAGAGGTGGTACCAGCTTTTGCTGAACTTGGCTTTATATGCACATGGACCAGCGATAATAAGATGGGATTTTCGATCGATCCAAAACTAGGGCCGCTTGAGGACACGAGTGTCTTCTTCAGGGCTGTGAATGCTTTAAATGCCTTTGCATATGGTTTTCCTGCACATTGGGTAATGACAGCAGATCTTTTGCGTTGTTTTCTTTTTCCTTTGTTCTTTCCGCACACTTATTTCTCTCCTTCACTGCAACAACATGTAAATAAATACTTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.53

Weight (kDa)

4.18

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
Acc65I GGTACC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 145
AciI CCGC 2 cut(s) 230, 356
AclWI GGATC 2 cut(s) 29, 209
AcuI CTGAAG 1 cut(s) 238
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 19, 224
AflIII ACRYGT 1 cut(s) 388
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
AlwI GGATC 2 cut(s) 29, 209
AoxI GGCC 2 cut(s) 10, 227
ApeKI GCWGC 2 cut(s) 64, 125
Asp718I GGTACC 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 183, 227
AvaII GGWCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 145
BauI CACGAG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 241
BbvI GCAGC 2 cut(s) 51, 112
BccI CCATC 1 cut(s) 193
BceAI ACGGC 1 cut(s) 25
BfaI CTAG 2 cut(s) 224, 409
BglII AGATCT 1 cut(s) 319
BisI GCNGC 3 cut(s) 65, 126, 230
BlsI GCNGC 3 cut(s) 66, 127, 231
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 183, 227
BmiI GGNNCC 1 cut(s) 147
BmsI GCATC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 81
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 224
Bse3DI GCAATG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 81
BseCI ATCGAT 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 19, 224
BseMI GCAATG 1 cut(s) 121
BseXI GCAGC 2 cut(s) 51, 112
BsgI GTGCAG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 12, 229
BshNI GGYRCC 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 213
BslI CCNNNNNNNGG 2 cut(s) 19, 224
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 2 cut(s) 12, 229
Bsp143I GATC 4 cut(s) 34, 210, 214, 319
BspACI CCGC 2 cut(s) 230, 356
BspANI GGCC 2 cut(s) 12, 229
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 147
BspPI GGATC 2 cut(s) 29, 209
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 121
BssMI GATC 4 cut(s) 34, 210, 214, 319
BssSI CACGAG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 69
BstENI CCTNNNNNAGG 1 cut(s) 17
BstKTI GATC 4 cut(s) 37, 213, 217, 322
BstMBI GATC 4 cut(s) 34, 210, 214, 319
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 174, 266, 275
BstNSI RCATGY 1 cut(s) 392
BstV1I GCAGC 2 cut(s) 51, 112
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 213
BsuRI GGCC 2 cut(s) 12, 229
BsuTUI ATCGAT 1 cut(s) 213
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 183, 227
ClaI ATCGAT 1 cut(s) 213
CseI GACGC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 146
CviAII CATG 3 cut(s) 86, 180, 389
CviJI RGCY 5 cut(s) 12, 152, 168, 229, 260
CviKI_1 RGCY 5 cut(s) 12, 152, 168, 229, 260
CviQI GTAC 1 cut(s) 146
DpnI GATC 4 cut(s) 36, 212, 216, 321
DpnII GATC 4 cut(s) 34, 210, 214, 319
DraI TTTAAA 1 cut(s) 273
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 17
FaeI CATG 3 cut(s) 89, 183, 392
FaiI YATR 8 cut(s) 82, 87, 173, 175, 181, 286, 288, 390
FatI CATG 3 cut(s) 85, 179, 388
FauNDI CATATG 1 cut(s) 286
Fnu4HI GCNGC 3 cut(s) 65, 126, 230
Fsp4HI GCNGC 3 cut(s) 65, 126, 230
FspBI CTAG 2 cut(s) 224, 409
GluI GCNGC 3 cut(s) 65, 126, 230
HaeIII GGCC 2 cut(s) 12, 229
HgaI GACGC 1 cut(s) 60
Hin1II CATG 3 cut(s) 89, 183, 392
HinfI GANTC 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 2 cut(s) 38, 101
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 1 cut(s) 384
HpyCH4V TGCA 6 cut(s) 67, 114, 177, 284, 299, 382
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 266, 275
Hsp92II CATG 3 cut(s) 89, 183, 392
KpnI GGTACC 1 cut(s) 149
Kzo9I GATC 4 cut(s) 34, 210, 214, 319
LpnPI CCDG 6 cut(s) 51, 114, 162, 199, 241, 309
Lsp1109I GCAGC 2 cut(s) 51, 112
LweI GCATC 1 cut(s) 112
MaeI CTAG 2 cut(s) 224, 409
MaeIII GTNAC 1 cut(s) 29
MalI GATC 4 cut(s) 36, 212, 216, 321
MboI GATC 4 cut(s) 34, 210, 214, 319
MboII GAAGA 2 cut(s) 241, 244
MflI RGATCY 1 cut(s) 319
MnlI CCTC 5 cut(s) 13, 23, 70, 133, 229
MseI TTAA 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 271
MwoI GCNNNNNNNGC 3 cut(s) 174, 266, 275
NdeI CATATG 1 cut(s) 286
NdeII GATC 4 cut(s) 34, 210, 214, 319
NlaIII CATG 3 cut(s) 89, 183, 392
NlaIV GGNNCC 1 cut(s) 147
NspI RCATGY 1 cut(s) 392
PciI ACATGT 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 66, 127, 231
Ple19I CGATCG 1 cut(s) 213
PscI ACATGT 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 2 cut(s) 183, 227
PsuI RGATCY 1 cut(s) 319
PvuI CGATCG 1 cut(s) 213
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 3 cut(s) 65, 126, 230
Sau3AI GATC 4 cut(s) 34, 210, 214, 319
Sau96I GGNCC 2 cut(s) 183, 227
SetI ASST 3 cut(s) 62, 144, 154
SfaNI GCATC 1 cut(s) 112
SinI GGWCC 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SsiI CCGC 2 cut(s) 230, 356
SspMI CTAG 2 cut(s) 224, 409
TaqI TCGA 2 cut(s) 209, 213
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 1 cut(s) 384
TseI GCWGC 2 cut(s) 64, 125
TspDTI ATGAA 1 cut(s) 69
TspRI CASTG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 17
XceI RCATGY 1 cut(s) 392
XspI CTAG 2 cut(s) 224, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.