Rh6BG171000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000575
Physical Location & Seq
Forward (+)
28219725 .. 28222088
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG171000.1

Sequence Viewer

Length: 513 bp
ATGGACGACGGCCTCAACGAGGACTTTGGTTACGATCGAGATATTTACTGCTTTGACACCTCGCTGCAAGCGTCCGATTCGTATCATGTGGACATCAATCCAGAAGTTATCTGCAATGATGATGCTGCTTGGGAAAAAGAGGTGGTACCAGCTTTTGCTGAACTTGGCTTTATATGCACATGGACCAGCGATAATAAGATGGGATTTTCGATCGATCCAAAACTAGGGCCGCTTGAGGACACGAGTGTCTTCTTCAGGGCCCGGCTTACTCACAACATCATCCTGTTTTATGATGAACCTGATATTTATCTTGTGGCAAGTAAACTATTAATTTTTCTATTGGGAGATTTCTTGGTGGAATTCATTAAGTTCGGTGGTGATTTTGAAAGCCTGGCCAAGGAGTTCTTGAAAGAGCATGAAAGACTAAAGGATCGCCCATTTCCATATGATGATACGATGGAGACTGGCAAAGCACCAAGTGGTGAACTGGGCACATGTAATCTCAGCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.34

Weight (kDa)

4.22

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
Acc65I GGTACC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 145
AciI CCGC 1 cut(s) 230
AclWI GGATC 2 cut(s) 209, 438
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 238
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 3 cut(s) 19, 224, 397
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 2 cut(s) 386, 409
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 2 cut(s) 209, 438
AlwNI CAGNNNCTG 1 cut(s) 510
AoxI GGCC 4 cut(s) 10, 227, 258, 393
ApaI GGGCCC 1 cut(s) 262
ApeKI GCWGC 2 cut(s) 64, 125
ApoI RAATTY 1 cut(s) 359
AseI ATTAAT 1 cut(s) 329
Asp718I GGTACC 1 cut(s) 145
AspS9I GGNCC 4 cut(s) 183, 227, 258, 259
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 389, 494
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 2 cut(s) 262, 494
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 262
BauI CACGAG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 241
BbvI GCAGC 2 cut(s) 51, 112
BccI CCATC 2 cut(s) 193, 451
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 1 cut(s) 392
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 1 cut(s) 224
BisI GCNGC 3 cut(s) 65, 126, 230
BlsI GCNGC 3 cut(s) 66, 127, 231
Bme1390I CCNGG 2 cut(s) 262, 392
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 183, 227, 258, 259
BmiI GGNNCC 2 cut(s) 147, 260
BmrFI CCNGG 2 cut(s) 262, 392
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 213
BsaBI GATNNNNATC 2 cut(s) 81, 306
BsaJI CCNNGG 1 cut(s) 396
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 224, 397
Bse1I ACTGG 2 cut(s) 469, 492
Bse3DI GCAATG 1 cut(s) 121
Bse8I GATNNNNATC 2 cut(s) 81, 306
BseBI CCWGG 1 cut(s) 392
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 396
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 2 cut(s) 81, 306
BseLI CCNNNNNNNGG 3 cut(s) 19, 224, 397
BseMI GCAATG 1 cut(s) 121
BseNI ACTGG 2 cut(s) 469, 492
BseSI GKGCMC 2 cut(s) 262, 494
BseXI GCAGC 2 cut(s) 51, 112
Bsh1285I CGRYCG 2 cut(s) 37, 213
BshFI GGCC 4 cut(s) 12, 229, 260, 395
BshNI GGYRCC 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 213
BsiEI CGRYCG 2 cut(s) 37, 213
BsiSI CCGG 1 cut(s) 262
BslI CCNNNNNNNGG 3 cut(s) 19, 224, 397
BsmAI GTCTC 1 cut(s) 455
BsnI GGCC 4 cut(s) 12, 229, 260, 395
Bsp120I GGGCCC 1 cut(s) 258
Bsp1286I GDGCHC 2 cut(s) 262, 494
Bsp143I GATC 4 cut(s) 34, 210, 214, 430
BspACI CCGC 1 cut(s) 230
BspANI GGCC 4 cut(s) 12, 229, 260, 395
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 147, 260
BspPI GGATC 2 cut(s) 209, 438
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 121
BsrI ACTGG 2 cut(s) 469, 492
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 4 cut(s) 34, 210, 214, 430
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 396
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 503
BstENI CCTNNNNNAGG 2 cut(s) 17, 395
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 4 cut(s) 37, 213, 217, 433
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 4 cut(s) 34, 210, 214, 430
BstMCI CGRYCG 2 cut(s) 37, 213
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 2 cut(s) 260, 390
BstSLI GKGCMC 2 cut(s) 262, 494
BstV1I GCAGC 2 cut(s) 51, 112
BstV2I GAAGAC 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 213
BsuRI GGCC 4 cut(s) 12, 229, 260, 395
BsuTUI ATCGAT 1 cut(s) 213
BtsCI GGATG 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 510
Cfr13I GGNCC 4 cut(s) 183, 227, 258, 259
ClaI ATCGAT 1 cut(s) 213
CseI GACGC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 146
CviAII CATG 4 cut(s) 86, 180, 416, 495
CviJI RGCY 8 cut(s) 12, 152, 168, 229, 260, 265, 390, 395
CviKI_1 RGCY 8 cut(s) 12, 152, 168, 229, 260, 265, 390, 395
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 1 cut(s) 503
DpnI GATC 4 cut(s) 36, 212, 216, 432
DpnII GATC 4 cut(s) 34, 210, 214, 430
DraIII CACNNNGTG 1 cut(s) 479
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 238
EcoNI CCTNNNNNAGG 2 cut(s) 17, 395
EcoO109I RGGNCCY 1 cut(s) 258
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 396
FaeI CATG 4 cut(s) 89, 183, 419, 498
FaiI YATR 9 cut(s) 87, 173, 175, 181, 291, 417, 445, 447, 496
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 4 cut(s) 85, 179, 415, 494
FauNDI CATATG 1 cut(s) 445
Fnu4HI GCNGC 3 cut(s) 65, 126, 230
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 3 cut(s) 65, 126, 230
FspBI CTAG 1 cut(s) 224
GluI GCNGC 3 cut(s) 65, 126, 230
HaeIII GGCC 4 cut(s) 12, 229, 260, 395
HapII CCGG 1 cut(s) 262
HgaI GACGC 1 cut(s) 60
Hin1II CATG 4 cut(s) 89, 183, 419, 498
HinfI GANTC 1 cut(s) 77
HpaII CCGG 1 cut(s) 262
HphI GGTGA 2 cut(s) 389, 494
Hpy166II GTNNAC 3 cut(s) 91, 323, 485
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 3 cut(s) 38, 101, 406
Hpy8I GTNNAC 3 cut(s) 91, 323, 485
Hpy99I CGWCG 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 67, 114, 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 1 cut(s) 503
Hsp92II CATG 4 cut(s) 89, 183, 419, 498
KpnI GGTACC 1 cut(s) 149
Kzo9I GATC 4 cut(s) 34, 210, 214, 430
Lsp1109I GCAGC 2 cut(s) 51, 112
LweI GCATC 1 cut(s) 112
MaeI CTAG 1 cut(s) 224
MaeIII GTNAC 1 cut(s) 29
MalI GATC 4 cut(s) 36, 212, 216, 432
MboI GATC 4 cut(s) 34, 210, 214, 430
MboII GAAGA 2 cut(s) 241, 244
MhlI GDGCHC 2 cut(s) 262, 494
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 2 cut(s) 330, 359
MluNI TGGCCA 1 cut(s) 395
MnlI CCTC 5 cut(s) 13, 23, 70, 133, 229
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
MseI TTAA 2 cut(s) 329, 366
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 2 cut(s) 262, 392
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 174
NciI CCSGG 1 cut(s) 262
NdeI CATATG 1 cut(s) 445
NdeII GATC 4 cut(s) 34, 210, 214, 430
NlaIII CATG 4 cut(s) 89, 183, 419, 498
NlaIV GGNNCC 2 cut(s) 147, 260
NspI RCATGY 1 cut(s) 498
PciI ACATGT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 68
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 66, 127, 231
Ple19I CGATCG 2 cut(s) 37, 213
PscI ACATGT 1 cut(s) 494
PshBI ATTAAT 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 147, 260
PspOMI GGGCCC 1 cut(s) 258
PspPI GGNCC 4 cut(s) 183, 227, 258, 259
PstNI CAGNNNCTG 1 cut(s) 510
PvuI CGATCG 2 cut(s) 37, 213
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
SaqAI TTAA 2 cut(s) 329, 366
SatI GCNGC 3 cut(s) 65, 126, 230
Sau3AI GATC 4 cut(s) 34, 210, 214, 430
Sau96I GGNCC 4 cut(s) 183, 227, 258, 259
ScrFI CCNGG 2 cut(s) 262, 392
SduI GDGCHC 2 cut(s) 262, 494
SetI ASST 5 cut(s) 62, 144, 154, 301, 512
SfaNI GCATC 1 cut(s) 112
SinI GGWCC 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 2 cut(s) 330, 359
SsiI CCGC 1 cut(s) 230
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 2 cut(s) 260, 390
StyI CCWWGG 1 cut(s) 396
TaqI TCGA 3 cut(s) 37, 209, 213
TasI AATT 2 cut(s) 330, 359
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 2 cut(s) 329, 366
Tru9I TTAA 2 cut(s) 329, 366
TseI GCWGC 2 cut(s) 64, 125
TspDTI ATGAA 3 cut(s) 309, 352, 432
VpaK11BI GGWCC 1 cut(s) 183
VspI ATTAAT 1 cut(s) 329
XagI CCTNNNNNAGG 2 cut(s) 17, 395
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 498
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.