RLG00000013513

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000575
Physical Location & Seq
Forward (+)
32548447 .. 32550269
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013513

Sequence Viewer

Length: 507 bp
ATGGACGGCGGCTTCATCTGCGATGAGGATTACTTCGGTTCCGATCCAGATGTTTTCCGCTTTGACCCCAGCGACTCCGACGAGGTGCAAGCGACCCGTCCGTTTCATGTGGACATCAATCCAGAAGTTATCTGCAATGATGATGCTGCTTGGGAAAAAGAGGTGGATAATAAGATGGGATTTTCGATCGATCCAAAACTAGGGCCGCTGGAGGACACGAGTGTCTTCTTCAGGGCTGCACTGAAACCACTGTCGGGCGATATGGAGTTCGATTCCTTCGAGCTGACACCCGCGGATGGGAGTACTAAGGAGCTTCAGGCGTTGCCCTGTGCAATTAGGTTTTATGATGAACCTGATATTTGTCTTGTGGCAAGTAAACTATTAATTTATCTACTGGTAGATTTCTCGGTGGAATTCATTAAGTGCGGTGGTGATTTTGAAAGCCTGGCCAAGGAGTTGTTGGAAGATCATGAAAGACTAAAGGATCACCCATTTCCATATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.92

Weight (kDa)

4.12

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccII CGCG 1 cut(s) 293
AciI CCGC 6 cut(s) 9, 58, 206, 291, 293, 426
AclWI GGATC 3 cut(s) 38, 185, 492
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 1 cut(s) 413
AcuI CTGAAG 2 cut(s) 214, 299
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 5 cut(s) 200, 254, 296, 297, 451
AgsI TTSAA 1 cut(s) 440
AjnI CCWGG 1 cut(s) 444
AluBI AGCT 2 cut(s) 283, 313
AluI AGCT 2 cut(s) 283, 313
AlwI GGATC 3 cut(s) 38, 185, 492
AoxI GGCC 2 cut(s) 203, 447
ApeKI GCWGC 2 cut(s) 146, 236
ApoI RAATTY 1 cut(s) 413
AseI ATTAAT 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 443, 479
BalI TGGCCA 1 cut(s) 449
BauI CACGAG 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 133, 223
BccI CCATC 2 cut(s) 169, 290
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 446
BfaI CTAG 1 cut(s) 200
BisI GCNGC 4 cut(s) 10, 147, 206, 237
BlsI GCNGC 4 cut(s) 11, 148, 207, 238
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 446
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 291, 450
Bsc4I CCNNNNNNNGG 5 cut(s) 200, 254, 296, 297, 451
Bse1I ACTGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 446
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 2 cut(s) 291, 450
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 5 cut(s) 200, 254, 296, 297, 451
BseMI GCAATG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 133, 223
BseYI CCCAGC 1 cut(s) 68
BsgI GTGCAG 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 293
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 205, 449
BshVI ATCGAT 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 189
BslI CCNNNNNNNGG 5 cut(s) 200, 254, 296, 297, 451
BsnI GGCC 2 cut(s) 205, 449
Bsp143I GATC 5 cut(s) 43, 186, 190, 466, 484
BspACI CCGC 6 cut(s) 9, 58, 206, 291, 293, 426
BspANI GGCC 2 cut(s) 205, 449
BspDI ATCGAT 1 cut(s) 189
BspFNI CGCG 1 cut(s) 293
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 3 cut(s) 38, 185, 492
BsrDI GCAATG 1 cut(s) 142
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 2 cut(s) 291, 450
BssMI GATC 5 cut(s) 43, 186, 190, 466, 484
BssSI CACGAG 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 291
BstENI CCTNNNNNAGG 1 cut(s) 449
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 293
BstKTI GATC 5 cut(s) 46, 189, 193, 469, 487
BstMBI GATC 5 cut(s) 43, 186, 190, 466, 484
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstNI CCWGG 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 444
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 133, 223
BstV2I GAAGAC 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 189
BsuRI GGCC 2 cut(s) 205, 449
BsuTUI ATCGAT 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 239, 248
Cac8I GCNNGC 1 cut(s) 90
CciI TCATGA 1 cut(s) 469
Cfr13I GGNCC 1 cut(s) 203
Cfr42I CCGCGG 1 cut(s) 294
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 1 cut(s) 303
CviAII CATG 2 cut(s) 107, 470
CviJI RGCY 7 cut(s) 12, 205, 236, 283, 313, 444, 449
CviKI_1 RGCY 7 cut(s) 12, 205, 236, 283, 313, 444, 449
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 1 cut(s) 306
DpnI GATC 5 cut(s) 45, 188, 192, 468, 486
DpnII GATC 5 cut(s) 43, 186, 190, 466, 484
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 450
Eco57I CTGAAG 2 cut(s) 214, 299
EcoNI CCTNNNNNAGG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 2 cut(s) 110, 473
FaiI YATR 6 cut(s) 108, 263, 345, 471, 499, 501
FatI CATG 2 cut(s) 106, 469
FauI CCCGC 1 cut(s) 298
FauNDI CATATG 1 cut(s) 499
Fnu4HI GCNGC 4 cut(s) 10, 147, 206, 237
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 4 cut(s) 10, 147, 206, 237
FspBI CTAG 1 cut(s) 200
GluI GCNGC 4 cut(s) 10, 147, 206, 237
GsaI CCCAGC 1 cut(s) 72
GsuI CTGGAG 1 cut(s) 230
HaeIII GGCC 2 cut(s) 205, 449
Hin1II CATG 2 cut(s) 110, 473
HinfI GANTC 2 cut(s) 74, 272
HphI GGTGA 2 cut(s) 443, 479
Hpy166II GTNNAC 2 cut(s) 112, 377
Hpy188I TCNGA 2 cut(s) 43, 79
Hpy188III TCNNGA 3 cut(s) 47, 122, 470
Hpy8I GTNNAC 2 cut(s) 112, 377
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4V TGCA 4 cut(s) 88, 135, 239, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 2 cut(s) 110, 473
KspI CCGCGG 1 cut(s) 294
Kzo9I GATC 5 cut(s) 43, 186, 190, 466, 484
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 2 cut(s) 133, 223
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 200
MalI GATC 5 cut(s) 45, 188, 192, 468, 486
MboI GATC 5 cut(s) 43, 186, 190, 466, 484
MboII GAAGA 3 cut(s) 217, 220, 476
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 3 cut(s) 333, 384, 413
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 2 cut(s) 102, 441
MnlI CCTC 4 cut(s) 19, 76, 154, 205
Mox20I TGGCCA 1 cut(s) 449
MscI TGGCCA 1 cut(s) 449
MseI TTAA 2 cut(s) 383, 420
MslI CAYNNNNRTG 1 cut(s) 502
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 208, 293
MspR9I CCNGG 1 cut(s) 446
MvaI CCWGG 1 cut(s) 446
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 1 cut(s) 18
NdeI CATATG 1 cut(s) 499
NdeII GATC 5 cut(s) 43, 186, 190, 466, 484
NlaIII CATG 2 cut(s) 110, 473
NlaIV GGNNCC 1 cut(s) 40
PagI TCATGA 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 11, 148, 207, 238
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PshBI ATTAAT 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 203
PvuI CGATCG 1 cut(s) 189
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 502
SacII CCGCGG 1 cut(s) 294
SaqAI TTAA 2 cut(s) 383, 420
SatI GCNGC 4 cut(s) 10, 147, 206, 237
Sau3AI GATC 5 cut(s) 43, 186, 190, 466, 484
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 304
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 446
SetI ASST 6 cut(s) 87, 165, 285, 315, 341, 355
SfaNI GCATC 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 294
SgrBI CCGCGG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 502
Sse9I AATT 3 cut(s) 333, 384, 413
SsiI CCGC 6 cut(s) 9, 58, 206, 291, 293, 426
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 1 cut(s) 252
TaqI TCGA 4 cut(s) 185, 189, 270, 279
TasI AATT 3 cut(s) 333, 384, 413
TatI WGTACW 1 cut(s) 302
TauI GCSGC 2 cut(s) 12, 208
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 2 cut(s) 383, 420
Tru9I TTAA 2 cut(s) 383, 420
TscAI CASTG 2 cut(s) 246, 255
TseI GCWGC 2 cut(s) 146, 236
TspDTI ATGAA 5 cut(s) 4, 95, 363, 406, 486
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 246, 255
VspI ATTAAT 1 cut(s) 383
XagI CCTNNNNNAGG 1 cut(s) 449
XapI RAATTY 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.