RLG00000014200

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000575
Physical Location & Seq
Reverse (-)
49246705 .. 49248560
1856 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014200

Sequence Viewer

Length: 672 bp
ATGTGTCCGGTGAATGGGATGGCTATGGAGCAGACTTCACAACCGAAGGGAACCCGATTGAACTCCCAGAAAGGGTTGTGCCTGATGCTTACAGGGAGTGGGAGGTTAAGGTTTTCGACTGAAGACCAAGTTCTTGCTTACAAGACGATAGAGCTACTTCCCACAGTTGGATGTGAAGCCGATGCTGCTACACAGTACAACATTCTTGAGAGGAATGTGGGTGGTGTGAACAACAAAGTTTCTGCCTTTGCGTATCAGTCTAATGGATGTTATGTGTCTGTTTTGCCAGTGGAGGATAAGAGTTTGAATAAGTTGTTGGAAGTAGAGTATTGCTTGATCAATCCTCAACATAAGGAGTCTCGTGTGAGGATAATTCAGGTGATTCGCATAGACAATATGAAGATGTGGTATGGGCCATTCAGAAATGGGGAGCAATTTGGTGGATATGTCATCCGTGATTCTGCTTTTGCTTCTACGCCCGCGTTGGAAGCTCCAAAAGTTGTTGGTGGGCATACTCCAGGATGGCAAGATGTGCAGCCTCATGATCCTCAAGTTCAAGATGCAGCCAACCATGTTGTGAAGAGCCTTCAACAAAAGTCCAATTCATTGTTCCCTTATGAGCTTCAAGAGATTGTCCATGCTAAGGCAGAGGTCAGTAGTGATTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

24.73

Weight (kDa)

6.21

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 482
AciI CCGC 1 cut(s) 480
AclWI GGATC 1 cut(s) 539
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 4 cut(s) 14, 72, 167, 643
AgsI TTSAA 5 cut(s) 61, 307, 557, 590, 626
AjnI CCWGG 1 cut(s) 517
AjuI GAANNNNNNNTTGG 4 cut(s) 299, 331, 593, 625
AluBI AGCT 3 cut(s) 154, 491, 622
AluI AGCT 3 cut(s) 154, 491, 622
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 539
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 3 cut(s) 185, 535, 563
AspS9I GGNCC 1 cut(s) 413
AsuHPI GGTGA 2 cut(s) 22, 391
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 172, 547, 575
BccI CCATC 2 cut(s) 13, 516
BciT130I CCWGG 1 cut(s) 519
BclI TGATCA 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 363
BisI GCNGC 3 cut(s) 186, 536, 564
BlsI GCNGC 3 cut(s) 187, 537, 565
Bme1390I CCNGG 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 3 cut(s) 75, 172, 550
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 501
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 2 cut(s) 227, 534
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 72, 167, 643
Bse1I ACTGG 1 cut(s) 287
BseBI CCWGG 1 cut(s) 519
BseGI GGATG 5 cut(s) 24, 176, 272, 450, 527
BseLI CCNNNNNNNGG 4 cut(s) 14, 72, 167, 643
BseNI ACTGG 1 cut(s) 287
BseXI GCAGC 3 cut(s) 172, 547, 575
BsgI GTGCAG 1 cut(s) 554
Bsh1236I CGCG 1 cut(s) 482
BshFI GGCC 1 cut(s) 415
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 4 cut(s) 14, 72, 167, 643
BsmAI GTCTC 1 cut(s) 363
BsnI GGCC 1 cut(s) 415
Bsp143I GATC 2 cut(s) 336, 544
BspACI CCGC 1 cut(s) 480
BspANI GGCC 1 cut(s) 415
BspFNI CGCG 1 cut(s) 482
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 52
BspPI GGATC 1 cut(s) 539
BspQI GCTCTTC 1 cut(s) 575
BsrI ACTGG 1 cut(s) 287
BssMI GATC 2 cut(s) 336, 544
BssSI CACGAG 1 cut(s) 360
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 2 cut(s) 166, 195
Bst6I CTCTTC 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 532
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 1 cut(s) 642
BstF5I GGATG 5 cut(s) 24, 176, 272, 450, 527
BstFNI CGCG 1 cut(s) 482
BstKTI GATC 2 cut(s) 339, 547
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 2 cut(s) 336, 544
BstMWI GCNNNNNNNGC 3 cut(s) 185, 488, 532
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 517
BstUI CGCG 1 cut(s) 482
BstV1I GCAGC 3 cut(s) 172, 547, 575
BstV2I GAAGAC 1 cut(s) 129
BsuRI GGCC 1 cut(s) 415
BtsCI GGATG 5 cut(s) 24, 176, 272, 450, 527
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 480
CciI TCATGA 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 1 cut(s) 196
CviAII CATG 3 cut(s) 542, 572, 638
CviJI RGCY 9 cut(s) 23, 154, 179, 415, 491, 538, 566, 585, 622
CviKI_1 RGCY 9 cut(s) 23, 154, 179, 415, 491, 538, 566, 585, 622
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 642
DpnI GATC 2 cut(s) 338, 546
DpnII GATC 2 cut(s) 336, 544
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 517
FaeI CATG 3 cut(s) 545, 575, 641
FatI CATG 3 cut(s) 541, 571, 637
FauI CCCGC 1 cut(s) 487
FbaI TGATCA 1 cut(s) 336
Fnu4HI GCNGC 3 cut(s) 186, 536, 564
FokI GGATG 5 cut(s) 31, 183, 279, 437, 534
Fsp4HI GCNGC 3 cut(s) 186, 536, 564
GluI GCNGC 3 cut(s) 186, 536, 564
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 1 cut(s) 415
HapII CCGG 1 cut(s) 8
Hin1II CATG 3 cut(s) 545, 575, 641
HinfI GANTC 3 cut(s) 356, 382, 458
HpaII CCGG 1 cut(s) 8
HphI GGTGA 2 cut(s) 22, 391
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 422
Hpy188III TCNNGA 4 cut(s) 206, 542, 557, 626
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 40, 596
HpyCH4III ACNGT 2 cut(s) 166, 195
HpyCH4V TGCA 2 cut(s) 535, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 488, 532
HpyF3I CTNAG 1 cut(s) 642
Hsp92II CATG 3 cut(s) 545, 575, 641
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 2 cut(s) 336, 544
LguI GCTCTTC 1 cut(s) 575
LmnI GCTCC 3 cut(s) 28, 430, 496
LpnPI CCDG 8 cut(s) 21, 78, 80, 95, 300, 362, 504, 531
Lsp1109I GCAGC 3 cut(s) 172, 547, 575
LweI GCATC 3 cut(s) 75, 172, 550
MalI GATC 2 cut(s) 338, 546
MboI GATC 2 cut(s) 336, 544
MboII GAAGA 3 cut(s) 134, 412, 592
MluCI AATT 3 cut(s) 372, 434, 601
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 3 cut(s) 148, 297, 465
MnlI CCTC 8 cut(s) 96, 204, 286, 354, 360, 549, 558, 643
MseI TTAA 1 cut(s) 107
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 3 cut(s) 185, 488, 532
NdeII GATC 2 cut(s) 336, 544
NlaIII CATG 3 cut(s) 545, 575, 641
NlaIV GGNNCC 1 cut(s) 52
PagI TCATGA 1 cut(s) 541
PciSI GCTCTTC 1 cut(s) 575
PfeI GAWTC 2 cut(s) 382, 458
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 3 cut(s) 187, 537, 565
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 413
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SapI GCTCTTC 1 cut(s) 575
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 3 cut(s) 186, 536, 564
Sau3AI GATC 2 cut(s) 336, 544
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 519
SetI ASST 7 cut(s) 107, 113, 156, 381, 493, 624, 654
SfaNI GCATC 3 cut(s) 75, 172, 550
SmlI CTYRAG 2 cut(s) 206, 549
SmoI CTYRAG 2 cut(s) 206, 549
Sse9I AATT 3 cut(s) 372, 434, 601
SsiI CCGC 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 517
TaaI ACNGT 2 cut(s) 166, 195
TaqI TCGA 1 cut(s) 116
TasI AATT 3 cut(s) 372, 434, 601
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 2 cut(s) 382, 458
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 1 cut(s) 294
TseI GCWGC 3 cut(s) 185, 535, 563
TspDTI ATGAA 2 cut(s) 413, 594
TspGWI ACGGA 1 cut(s) 443
TspRI CASTG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.