MD02G1201600.v1.1

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012325
Physical Location & Seq
Reverse (-)
19920959 .. 19923672
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1201600.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGCTACGTTGAAGAGCTGCAGCGTGTGTTTGCGAGAGTCTGCTCTCCAGCTTCCGCGTCACCATCTTAGCGATAACTACAAGAGGCGGAATACAGAATGGAGGTGTCCACGGGCAGCTGTAATTCCCAATTTTCATCTGCCAATGCGTAGTTTTGAAGTTAAAAACAGGACATCGGTGGATGACATAAAGTCCATTAGGTTGATAACTGCCATCAAAACCCCATATCTACCAGATGGCAGATTTGATCTTGAAGCATACGATGCTTTGGTCAATATGCAGATTGAAAATGGAGCTGAAGGTGTGATTGTTGGTGGCACGACTGGTGAAGGCCAATTGATGAGCTGGGATGAGCATATAATGCTCATTGGCCACACGATCAACTGCTATGGTGGATCAATTAAGGTAATAGGAAACACAGGAAGTAATTCTACTAGAGAAGCAATTCATGCCACCGAACAGGGTTTTGCTGTTGGAATGCATGCTGCCCTTCACATCAATCCTTATTATGGGAAAACCTCCTTGGAGGGATTGGTTGCGCACCTCGATAGTGTTTTATCTATGGGACCCACAATTGTTTACAATGTGCCATCACGAACCGGCCAAGATATCCCTCCAAGCGTGGTTCACACGTTGGCACAAAGTCCTAACTTCGCAGGTATCAAGGAGTGTGTTGGAACTGATCGGGTTCAAGAGTACACTGGGAAGAAAATTGTGGTATGGAGTGGGAATGATGATGAGTGCCATGATTCTAGGTGGAGCCATGGAGCTACCGGAGTCATATCTGTTACTAGTAACTTGGTTCCAGGTTTAATGCGTCAGCTCATGTTTGGGGGGAAGAATCCGTCTCTGAATGCAAAAATTATGCCTCTGGTTAAGTGGCTTTTCCAAGAGCCAAATCCCATCGGCTTAAACACAGCACTTGCCCAACTTGGTGTTGTGAGGCCGGTCTTCAGGCTGCCATATGTACCTCTTCCTCTTGCAAAGAGGGTAGAGTTTGTGAACTTGATTAAGCAAATTGGGCGGGAGAATTTTGTCGGAGAAAAGGATGTCCGAGTTCTTGATGATGATGAGTTCATCTTAGTCAGTCGATATTGA

Protein Analysis

366

Amino Acids

40.34

Weight (kDa)

7.69

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHDPS PF00701 66 - 339 4.5e-93 Dihydrodipicolinate synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45440 AT3G60880 AT3G60880
fragaria_vesca FvH4_7g12060
malus_domestica MD02G1201600.v1.1
prunus_persica Prupe.2G147200_v2.0.a1
pyrus_communis pycom02g16500
rosa_chinensis RchiOBHm_Chr1g0350811 RchiOBHm_Chr7g0217051
rosa_laevigata RLG00000028454
rosa_multiflora Rmu_co8411727.1_g000001 Rmu_sc0006200.1_g000005 Rmu_sc0008591.1_g000006
rosa_roxburghii Rroxscaffold_4G00304500
rosa_rugosa Rorug01G0214100
rosa_samantha Rh1BG198700 Rh1CG213500 Rh1DG226600
rosa_wichuraiana Rw1G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 647
AccII CGCG 1 cut(s) 58
AciI CCGC 3 cut(s) 56, 88, 1024
AclWI GGATC 1 cut(s) 403
AcoI YGGCCR 2 cut(s) 370, 601
AcsI RAATTY 1 cut(s) 1030
AcuI CTGAAG 2 cut(s) 318, 937
AfaI GTAC 2 cut(s) 698, 969
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 5 cut(s) 13, 158, 254, 287, 692
AhdI GACNNNNNGTC 1 cut(s) 190
AhlI ACTAGT 1 cut(s) 791
AjnI CCWGG 1 cut(s) 805
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 7 cut(s) 18, 52, 119, 296, 345, 770, 823
AluI AGCT 7 cut(s) 18, 52, 119, 296, 345, 770, 823
Alw26I GTCTC 1 cut(s) 852
AlwI GGATC 1 cut(s) 403
AoxI GGCC 4 cut(s) 331, 370, 601, 944
ApeKI GCWGC 5 cut(s) 18, 21, 116, 485, 958
ApoI RAATTY 1 cut(s) 1030
Asp700I GAANNNNTTC 2 cut(s) 427, 444
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 566
AsuHPI GGTGA 2 cut(s) 53, 338
AvaII GGWCC 1 cut(s) 566
BalI TGGCCA 1 cut(s) 372
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
BbsI GAAGAC 1 cut(s) 943
BbvI GCAGC 5 cut(s) 5, 33, 128, 472, 945
BccI CCATC 5 cut(s) 72, 221, 230, 598, 911
BciT130I CCWGG 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 852
BcuI ACTAGT 1 cut(s) 791
BfaI CTAG 3 cut(s) 435, 753, 792
BfmI CTRYAG 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 647
BisI GCNGC 5 cut(s) 19, 22, 117, 486, 959
BlsI GCNGC 5 cut(s) 20, 23, 118, 487, 960
Bme1390I CCNGG 1 cut(s) 807
Bme18I GGWCC 1 cut(s) 566
BmeRI GACNNNNNGTC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 4 cut(s) 567, 568, 761, 804
BmrFI CCNGG 1 cut(s) 807
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 711
BpiI GAAGAC 1 cut(s) 943
BpmI CTGGAG 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 110, 522, 763
BsaWI WCCGGW 1 cut(s) 773
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse118I RCCGGY 2 cut(s) 599, 946
Bse1I ACTGG 2 cut(s) 328, 706
BseBI CCWGG 1 cut(s) 807
BseDI CCNNGG 3 cut(s) 110, 522, 763
BseGI GGATG 3 cut(s) 187, 355, 1054
BseLI CCNNNNNNNGG 1 cut(s) 509
BseNI ACTGG 2 cut(s) 328, 706
BseXI GCAGC 5 cut(s) 5, 33, 128, 472, 945
BseYI CCCAGC 1 cut(s) 345
Bsh1236I CGCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 333, 372, 603, 946
BsiSI CCGG 3 cut(s) 600, 774, 947
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 852
BsmBI CGTCTC 1 cut(s) 852
BsmFI GGGAC 1 cut(s) 579
BsmI GAATGC 2 cut(s) 483, 859
BsnI GGCC 4 cut(s) 333, 372, 603, 946
Bsp143I GATC 4 cut(s) 247, 378, 395, 682
Bsp19I CCATGG 1 cut(s) 763
BspACI CCGC 3 cut(s) 56, 88, 1024
BspANI GGCC 4 cut(s) 333, 372, 603, 946
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 4 cut(s) 567, 568, 761, 804
BspMAI CTGCAG 1 cut(s) 23
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 1 cut(s) 403
BspQI GCTCTTC 1 cut(s) 8
BsrFI RCCGGY 2 cut(s) 599, 946
BsrI ACTGG 2 cut(s) 328, 706
BssAI RCCGGY 2 cut(s) 599, 946
BssECI CCNNGG 3 cut(s) 110, 522, 763
BssMI GATC 4 cut(s) 247, 378, 395, 682
BssT1I CCWWGG 2 cut(s) 522, 763
Bst2UI CCWGG 1 cut(s) 807
Bst6I CTCTTC 2 cut(s) 8, 978
BstAPI GCANNNNNTGC 3 cut(s) 263, 361, 449
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 2 cut(s) 68, 1081
BstDSI CCRYGG 2 cut(s) 110, 763
BstF5I GGATG 3 cut(s) 187, 355, 1054
BstFNI CGCG 1 cut(s) 58
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 4 cut(s) 250, 381, 398, 685
BstMAI GTCTC 1 cut(s) 852
BstMBI GATC 4 cut(s) 247, 378, 395, 682
BstMWI GCNNNNNNNGC 4 cut(s) 263, 361, 449, 1021
BstNI CCWGG 1 cut(s) 807
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 805
BstSFI CTRYAG 1 cut(s) 19
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 5 cut(s) 5, 33, 128, 472, 945
BstV2I GAAGAC 1 cut(s) 943
BsuRI GGCC 4 cut(s) 333, 372, 603, 946
BtgI CCRYGG 2 cut(s) 110, 763
BtsCI GGATG 3 cut(s) 187, 355, 1054
BtsIMutI CAGTG 1 cut(s) 699
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 1 cut(s) 541
Cfr10I RCCGGY 2 cut(s) 599, 946
Cfr13I GGNCC 1 cut(s) 566
CseI GACGC 2 cut(s) 47, 806
Csp6I GTAC 2 cut(s) 697, 968
CviAII CATG 5 cut(s) 449, 482, 746, 764, 826
CviQI GTAC 2 cut(s) 697, 968
DdeI CTNAG 2 cut(s) 68, 1081
DpnI GATC 4 cut(s) 249, 380, 397, 684
DpnII GATC 4 cut(s) 247, 378, 395, 682
DriI GACNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 2 cut(s) 370, 601
Eam1104I CTCTTC 2 cut(s) 8, 978
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 2 cut(s) 8, 978
EciI GGCGGA 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 522, 763
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 566
Eco57I CTGAAG 2 cut(s) 318, 937
EcoO109I RGGNCCY 1 cut(s) 566
EcoRII CCWGG 1 cut(s) 805
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 2 cut(s) 522, 763
EcoT22I ATGCAT 1 cut(s) 483
ErhI CCWWGG 2 cut(s) 522, 763
Esp3I CGTCTC 1 cut(s) 852
FaeI CATG 5 cut(s) 452, 485, 749, 767, 829
FaqI GGGAC 1 cut(s) 579
FatI CATG 5 cut(s) 448, 481, 745, 763, 825
FauI CCCGC 1 cut(s) 1017
FauNDI CATATG 1 cut(s) 964
Fnu4HI GCNGC 5 cut(s) 19, 22, 117, 486, 959
FokI GGATG 3 cut(s) 194, 362, 1061
Fsp4HI GCNGC 5 cut(s) 19, 22, 117, 486, 959
FspBI CTAG 3 cut(s) 435, 753, 792
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 1 cut(s) 540
GluI GCNGC 5 cut(s) 19, 22, 117, 486, 959
GsaI CCCAGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 4 cut(s) 333, 372, 603, 946
HapII CCGG 3 cut(s) 600, 774, 947
HgaI GACGC 2 cut(s) 47, 806
HhaI GCGC 1 cut(s) 541
Hin1II CATG 5 cut(s) 452, 485, 749, 767, 829
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HinfI GANTC 4 cut(s) 38, 749, 777, 841
HpaII CCGG 3 cut(s) 600, 774, 947
HphI GGTGA 2 cut(s) 53, 338
Hpy166II GTNNAC 5 cut(s) 110, 580, 628, 699, 1003
Hpy188I TCNGA 3 cut(s) 852, 1040, 1055
Hpy188III TCNNGA 4 cut(s) 251, 594, 692, 1061
Hpy8I GTNNAC 5 cut(s) 110, 580, 628, 699, 1003
HpyAV CCTTC 3 cut(s) 293, 323, 500
HpyCH4IV ACGT 2 cut(s) 8, 632
HpyCH4V TGCA 5 cut(s) 21, 280, 481, 857, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 361, 449, 1021
HpyF3I CTNAG 2 cut(s) 68, 1081
HpySE526I ACGT 2 cut(s) 8, 632
Hsp92II CATG 5 cut(s) 452, 485, 749, 767, 829
HspAI GCGC 1 cut(s) 539
KflI GGGWCCC 1 cut(s) 566
Kzo9I GATC 4 cut(s) 247, 378, 395, 682
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 3 cut(s) 293, 759, 767
Lsp1109I GCAGC 5 cut(s) 5, 33, 128, 472, 945
LweI GCATC 1 cut(s) 253
MaeI CTAG 3 cut(s) 435, 753, 792
MaeII ACGT 2 cut(s) 8, 632
MaeIII GTNAC 3 cut(s) 59, 787, 794
MalI GATC 4 cut(s) 249, 380, 397, 684
MboI GATC 4 cut(s) 247, 378, 395, 682
MboII GAAGA 5 cut(s) 25, 718, 850, 943, 965
MfeI CAATTG 2 cut(s) 335, 573
MlsI TGGCCA 1 cut(s) 372
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 2 cut(s) 47, 786
MmeI TCCRAC 3 cut(s) 454, 655, 1018
Mox20I TGGCCA 1 cut(s) 372
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 2 cut(s) 427, 444
MscI TGGCCA 1 cut(s) 372
MseI TTAA 6 cut(s) 162, 402, 812, 876, 911, 1011
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 3 cut(s) 600, 774, 947
MspR9I CCNGG 1 cut(s) 807
MunI CAATTG 2 cut(s) 335, 573
Mva1269I GAATGC 2 cut(s) 483, 859
MvaI CCWGG 1 cut(s) 807
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 4 cut(s) 263, 361, 449, 1021
NcoI CCATGG 1 cut(s) 763
NdeI CATATG 1 cut(s) 964
NdeII GATC 4 cut(s) 247, 378, 395, 682
NlaIII CATG 5 cut(s) 452, 485, 749, 767, 829
NlaIV GGNNCC 4 cut(s) 567, 568, 761, 804
NmuCI GTSAC 1 cut(s) 59
NsbI TGCGCA 1 cut(s) 540
NsiI ATGCAT 1 cut(s) 483
NspI RCATGY 1 cut(s) 485
PaeI GCATGC 1 cut(s) 485
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 2 cut(s) 483, 859
PdmI GAANNNNTTC 2 cut(s) 427, 444
PfeI GAWTC 2 cut(s) 749, 841
PkrI GCNGC 5 cut(s) 20, 23, 118, 487, 960
PleI GAGTC 2 cut(s) 46, 785
PpsI GAGTC 2 cut(s) 46, 785
PpuMI RGGWCCY 1 cut(s) 566
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 805
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 1 cut(s) 805
PspN4I GGNNCC 4 cut(s) 567, 568, 761, 804
PspPI GGNCC 1 cut(s) 566
PspPPI RGGWCCY 1 cut(s) 566
PstI CTGCAG 1 cut(s) 23
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 2 cut(s) 698, 969
RsaNI GTAC 2 cut(s) 697, 968
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 6 cut(s) 162, 402, 812, 876, 911, 1011
SatI GCNGC 5 cut(s) 19, 22, 117, 486, 959
Sau3AI GATC 4 cut(s) 247, 378, 395, 682
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 2 cut(s) 47, 786
ScrFI CCNGG 1 cut(s) 807
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 19
SinI GGWCC 1 cut(s) 566
SpeI ACTAGT 1 cut(s) 791
SphI GCATGC 1 cut(s) 485
SsiI CCGC 3 cut(s) 56, 88, 1024
SspMI CTAG 3 cut(s) 435, 753, 792
StyD4I CCNGG 1 cut(s) 805
StyI CCWWGG 2 cut(s) 522, 763
TaiI ACGT 2 cut(s) 11, 635
TaqI TCGA 2 cut(s) 546, 1090
TatI WGTACW 1 cut(s) 696
TfiI GAWTC 2 cut(s) 749, 841
Tru1I TTAA 6 cut(s) 162, 402, 812, 876, 911, 1011
Tru9I TTAA 6 cut(s) 162, 402, 812, 876, 911, 1011
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 5 cut(s) 18, 21, 116, 485, 958
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 3 cut(s) 125, 437, 1066
TspGWI ACGGA 1 cut(s) 834
TspRI CASTG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 566
XapI RAATTY 1 cut(s) 1030
XceI RCATGY 1 cut(s) 485
XmnI GAANNNNTTC 2 cut(s) 427, 444
XspI CTAG 3 cut(s) 435, 753, 792
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.