Rh1CG213500

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0012325
Physical Location & Seq
Forward (+)
46025074 .. 46027463
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG213500.1

Sequence Viewer

Length: 1101 bp
ATGGCGAGTTTGCAGAGCTGCAGCAGCGTGTGCCTGAGAGAGTCGGCTCTCTTGCTTCCGCGTCGCAATGTTAACGATAGCTACAAGAGTAGGACTGTAAAATGGAGGAGTCCACGAGGAGCTGTGCTGCCCAATTTTCACCTTCCGATGCGTAGTTTCGAAATTAAAAACAGGACATCTACAGAGGAGATAAAGTCACTTAGACTGATAACAGCCATCAAAACGCCATATCTACCGGATGGCAGATTTGATCTTGAAGCGTATGATGCATTGGTTAATACGCAGATTGAACATGGAGCTGAAGGTGTGATTGTTGGTGGCACAACGGGTGAAGGTCAGTTGATGAGTTGGGACGAGCACATAATGCTCATAGGCCACACGATCAACTGTTATGGTGGATCAATCAAGGTAATAGGAAACACTGGCAGCAACTCCACAAGGGAAGCAGTTCATGCTACTGAACAAGGGTTTGCAGTTGGGATGCATGCTGCACTGCACATCAATCCTTATTATGGAAAGACCTCCTTGGAGGGATTGGTTAAGCACTTCGAAAGGGTCTTATCTATGGGACCCACTATCATATACAACGTGCCATCACGAACTGGCCAAGATATCCCCCACCAGGTGATTAACACGTTGGCACAGAATGCCAACTTGGCGGGTGTCAAGGAGTGCGTTGGAAATGATCGGATTATAGAGCACATTGACAAGGGAATTGTGGTTTGGAGTGGGAATGATGATGAGTGCCATGATTCAAGGTGGAACCACGGAGCTACTGGAGTCATATCTGTTACTAGTAATTTGGTTCCGGGTTTAATGCGAGACCTCATGTTTGGAGGGAAGAACCCTTCTCTTAATGCAAAGCTCATGCCTCTGATTGAGTGGCTTTTTCAGGAGCCAAACCCCATTGGCCTAAATACAGCACTTGCCCAACTTGGTGTCGTGAGACCAGTCTTCAGGCTGCCATATGTACCTCTTCCTTATGAAAAGAGAGTAGATTTTGTCAACCTGGTTAAGCAAATTGGGCGGGAGCATTTTGTTGGAGAAAAGGGTGTTCAAGTTCTTGATGATGATGACTTCACTTATTTGGATCGATATTAG

Protein Analysis

366

Amino Acids

40.6

Weight (kDa)

6.5

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHDPS PF00701 67 - 340 1.4e-93 Dihydrodipicolinate synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45440 AT3G60880 AT3G60880
fragaria_vesca FvH4_7g12060
malus_domestica MD02G1201600.v1.1
prunus_persica Prupe.2G147200_v2.0.a1
pyrus_communis pycom02g16500
rosa_chinensis RchiOBHm_Chr1g0350811 RchiOBHm_Chr7g0217051
rosa_laevigata RLG00000028454
rosa_multiflora Rmu_co8411727.1_g000001 Rmu_sc0006200.1_g000005 Rmu_sc0008591.1_g000006
rosa_roxburghii Rroxscaffold_4G00304500
rosa_rugosa Rorug01G0214100
rosa_samantha Rh1BG198700 Rh1CG213500 Rh1DG226600
rosa_wichuraiana Rw1G019790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 61
AciI CCGC 3 cut(s) 59, 659, 1027
AclWI GGATC 2 cut(s) 406, 1098
AcoI YGGCCR 1 cut(s) 604
AcuI CTGAAG 2 cut(s) 321, 940
AdeI CACNNNGTG 1 cut(s) 625
AfaI GTAC 1 cut(s) 972
AfiI CCNNNNNNNGG 2 cut(s) 512, 622
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 4 cut(s) 257, 290, 756, 1058
AhlI ACTAGT 1 cut(s) 794
AjnI CCWGG 2 cut(s) 621, 1008
AjuI GAANNNNNNNTTGG 4 cut(s) 638, 670, 706, 738
AluBI AGCT 6 cut(s) 18, 81, 122, 299, 773, 865
AluI AGCT 6 cut(s) 18, 81, 122, 299, 773, 865
Alw21I GWGCWC 2 cut(s) 360, 702
Alw26I GTCTC 2 cut(s) 816, 940
AlwI GGATC 2 cut(s) 406, 1098
AoxI GGCC 3 cut(s) 373, 604, 910
ApeKI GCWGC 7 cut(s) 18, 21, 24, 127, 426, 488, 961
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 569
AsuC2I CCSGG 1 cut(s) 810
AsuHPI GGTGA 3 cut(s) 131, 341, 637
AsuII TTCGAA 2 cut(s) 159, 549
AvaII GGWCC 1 cut(s) 569
BalI TGGCCA 1 cut(s) 606
BauI CACGAG 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 946
Bbv12I GWGCWC 2 cut(s) 360, 702
BbvI GCAGC 7 cut(s) 5, 33, 36, 114, 438, 475, 948
BccI CCATC 3 cut(s) 224, 233, 601
BciT130I CCWGG 2 cut(s) 623, 1010
BcnI CCSGG 1 cut(s) 810
BcoDI GTCTC 2 cut(s) 816, 940
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 2 cut(s) 19, 180
BglI GCCNNNNNGGC 1 cut(s) 656
BisI GCNGC 7 cut(s) 19, 22, 25, 128, 427, 489, 962
BlsI GCNGC 7 cut(s) 20, 23, 26, 129, 428, 490, 963
Bme1390I CCNGG 3 cut(s) 623, 810, 1010
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 5 cut(s) 570, 571, 764, 807, 897
BmrFI CCNGG 3 cut(s) 623, 810, 1010
BmsI GCATC 3 cut(s) 138, 256, 471
BpiI GAAGAC 1 cut(s) 946
BpmI CTGGAG 1 cut(s) 798
Bpu14I TTCGAA 2 cut(s) 159, 549
BpuMI CCSGG 1 cut(s) 810
Bsa29I ATCGAT 1 cut(s) 1093
BsaI GGTCTC 2 cut(s) 816, 940
BsaJI CCNNGG 2 cut(s) 525, 766
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 622
Bse1I ACTGG 4 cut(s) 427, 607, 781, 950
Bse3DI GCAATG 1 cut(s) 73
BseBI CCWGG 2 cut(s) 623, 1010
BseCI ATCGAT 1 cut(s) 1093
BseDI CCNNGG 2 cut(s) 525, 766
BseGI GGATG 2 cut(s) 244, 486
BseLI CCNNNNNNNGG 2 cut(s) 512, 622
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 4 cut(s) 427, 607, 781, 950
BseRI GAGGAG 3 cut(s) 121, 132, 200
BseXI GCAGC 7 cut(s) 5, 33, 36, 114, 438, 475, 948
BsgI GTGCAG 2 cut(s) 474, 479
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 3 cut(s) 375, 606, 912
BshVI ATCGAT 1 cut(s) 1093
BsiHKAI GWGCWC 2 cut(s) 360, 702
BsiSI CCGG 2 cut(s) 236, 809
BslFI GGGAC 2 cut(s) 365, 582
BslI CCNNNNNNNGG 2 cut(s) 512, 622
BsmAI GTCTC 2 cut(s) 816, 940
BsmFI GGGAC 2 cut(s) 365, 582
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 3 cut(s) 375, 606, 912
Bso31I GGTCTC 2 cut(s) 816, 940
Bsp119I TTCGAA 2 cut(s) 159, 549
Bsp1286I GDGCHC 2 cut(s) 360, 702
Bsp143I GATC 5 cut(s) 250, 381, 398, 685, 1090
BspACI CCGC 3 cut(s) 59, 659, 1027
BspANI GGCC 3 cut(s) 375, 606, 912
BspCNI CTCAG 1 cut(s) 27
BspDI ATCGAT 1 cut(s) 1093
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 5 cut(s) 570, 571, 764, 807, 897
BspMAI CTGCAG 1 cut(s) 23
BspPI GGATC 2 cut(s) 406, 1098
BspT104I TTCGAA 2 cut(s) 159, 549
BspTNI GGTCTC 2 cut(s) 816, 940
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 4 cut(s) 427, 607, 781, 950
BssECI CCNNGG 2 cut(s) 525, 766
BssMI GATC 5 cut(s) 250, 381, 398, 685, 1090
BssSI CACGAG 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 623, 1010
Bst4CI ACNGT 2 cut(s) 97, 389
Bst6I CTCTTC 1 cut(s) 981
BstAPI GCANNNNNTGC 4 cut(s) 30, 364, 452, 647
BstBI TTCGAA 2 cut(s) 159, 549
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 2 cut(s) 35, 200
BstDSI CCRYGG 1 cut(s) 766
BstF5I GGATG 2 cut(s) 244, 486
BstFNI CGCG 1 cut(s) 61
BstKTI GATC 5 cut(s) 253, 384, 401, 688, 1093
BstMAI GTCTC 2 cut(s) 816, 940
BstMBI GATC 5 cut(s) 250, 381, 398, 685, 1090
BstMWI GCNNNNNNNGC 8 cut(s) 24, 30, 266, 364, 452, 647, 656, 1024
BstNI CCWGG 2 cut(s) 623, 1010
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 621, 808, 1008
BstSFI CTRYAG 2 cut(s) 19, 180
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 7 cut(s) 5, 33, 36, 114, 438, 475, 948
BstV2I GAAGAC 1 cut(s) 946
Bsu15I ATCGAT 1 cut(s) 1093
BsuRI GGCC 3 cut(s) 375, 606, 912
BsuTUI ATCGAT 1 cut(s) 1093
BtgI CCRYGG 1 cut(s) 766
BtsCI GGATG 2 cut(s) 244, 486
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 2 cut(s) 420, 491
Cac8I GCNNGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 569
ClaI ATCGAT 1 cut(s) 1093
CseI GACGC 1 cut(s) 50
CsiI ACCWGGT 2 cut(s) 621, 1008
Csp6I GTAC 1 cut(s) 971
CviAII CATG 6 cut(s) 293, 452, 485, 749, 829, 868
CviQI GTAC 1 cut(s) 971
DdeI CTNAG 2 cut(s) 35, 200
DpnI GATC 5 cut(s) 252, 383, 400, 687, 1092
DpnII GATC 5 cut(s) 250, 381, 398, 685, 1090
DraIII CACNNNGTG 1 cut(s) 625
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 1 cut(s) 981
EarI CTCTTC 1 cut(s) 981
Eco130I CCWWGG 1 cut(s) 525
Eco31I GGTCTC 2 cut(s) 816, 940
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 2 cut(s) 321, 940
EcoO109I RGGNCCY 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 621, 1008
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 525
EcoT22I ATGCAT 2 cut(s) 271, 486
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 6 cut(s) 296, 455, 488, 752, 832, 871
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FaqI GGGAC 2 cut(s) 365, 582
FatI CATG 6 cut(s) 292, 451, 484, 748, 828, 867
FauI CCCGC 2 cut(s) 652, 1020
FauNDI CATATG 1 cut(s) 967
Fnu4HI GCNGC 7 cut(s) 19, 22, 25, 128, 427, 489, 962
FokI GGATG 2 cut(s) 251, 493
Fsp4HI GCNGC 7 cut(s) 19, 22, 25, 128, 427, 489, 962
FspBI CTAG 1 cut(s) 795
GluI GCNGC 7 cut(s) 19, 22, 25, 128, 427, 489, 962
GsuI CTGGAG 1 cut(s) 798
HaeIII GGCC 3 cut(s) 375, 606, 912
HapII CCGG 2 cut(s) 236, 809
HgaI GACGC 1 cut(s) 50
Hin1II CATG 6 cut(s) 296, 455, 488, 752, 832, 871
HincII GTYRAC 2 cut(s) 73, 1006
HindII GTYRAC 2 cut(s) 73, 1006
HinfI GANTC 4 cut(s) 41, 109, 752, 780
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 2 cut(s) 236, 809
HphI GGTGA 3 cut(s) 131, 341, 637
Hpy166II GTNNAC 3 cut(s) 73, 113, 1006
Hpy188I TCNGA 3 cut(s) 147, 690, 876
Hpy188III TCNNGA 5 cut(s) 254, 597, 893, 943, 1064
Hpy8I GTNNAC 3 cut(s) 73, 113, 1006
Hpy99I CGWCG 1 cut(s) 66
HpyAV CCTTC 4 cut(s) 152, 296, 326, 858
HpyCH4III ACNGT 2 cut(s) 97, 389
HpyCH4IV ACGT 2 cut(s) 588, 635
HpyCH4V TGCA 8 cut(s) 13, 21, 269, 473, 484, 491, 496, 860
HpyF10VI GCNNNNNNNGC 8 cut(s) 24, 30, 266, 364, 452, 647, 656, 1024
HpyF3I CTNAG 2 cut(s) 35, 200
HpySE526I ACGT 2 cut(s) 588, 635
Hsp92II CATG 6 cut(s) 296, 455, 488, 752, 832, 871
KflI GGGWCCC 1 cut(s) 569
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 5 cut(s) 250, 381, 398, 685, 1090
LmnI GCTCC 5 cut(s) 119, 296, 770, 895, 1030
Lsp1109I GCAGC 7 cut(s) 5, 33, 36, 114, 438, 475, 948
LweI GCATC 3 cut(s) 138, 256, 471
MabI ACCWGGT 2 cut(s) 621, 1008
MaeI CTAG 1 cut(s) 795
MaeII ACGT 2 cut(s) 588, 635
MaeIII GTNAC 2 cut(s) 195, 790
MalI GATC 5 cut(s) 252, 383, 400, 687, 1092
MboI GATC 5 cut(s) 250, 381, 398, 685, 1090
MboII GAAGA 3 cut(s) 853, 946, 968
MhlI GDGCHC 2 cut(s) 360, 702
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 5 cut(s) 133, 162, 714, 799, 1020
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 3 cut(s) 50, 118, 789
MmeI TCCRAC 2 cut(s) 658, 1021
MnlI CCTC 9 cut(s) 99, 110, 178, 523, 532, 830, 836, 882, 984
Mox20I TGGCCA 1 cut(s) 606
Mph1103I ATGCAT 2 cut(s) 271, 486
MroXI GAANNNNTTC 1 cut(s) 447
MscI TGGCCA 1 cut(s) 606
MseI TTAA 8 cut(s) 72, 165, 276, 540, 630, 815, 855, 1014
Msp20I TGGCCA 1 cut(s) 606
MspI CCGG 2 cut(s) 236, 809
MspR9I CCNGG 3 cut(s) 623, 810, 1010
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 2 cut(s) 623, 1010
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 8 cut(s) 24, 30, 266, 364, 452, 647, 656, 1024
NciI CCSGG 1 cut(s) 810
NdeI CATATG 1 cut(s) 967
NdeII GATC 5 cut(s) 250, 381, 398, 685, 1090
NlaIII CATG 6 cut(s) 296, 455, 488, 752, 832, 871
NlaIV GGNNCC 5 cut(s) 570, 571, 764, 807, 897
NmuCI GTSAC 1 cut(s) 195
NsiI ATGCAT 2 cut(s) 271, 486
NspI RCATGY 1 cut(s) 488
NspV TTCGAA 2 cut(s) 159, 549
PaeI GCATGC 1 cut(s) 488
PctI GAATGC 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 752
PkrI GCNGC 7 cut(s) 20, 23, 26, 129, 428, 490, 963
PleI GAGTC 3 cut(s) 49, 117, 788
PpsI GAGTC 3 cut(s) 49, 117, 788
PpuMI RGGWCCY 1 cut(s) 569
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 2 cut(s) 621, 1008
PspGI CCWGG 2 cut(s) 621, 1008
PspN4I GGNNCC 5 cut(s) 570, 571, 764, 807, 897
PspPI GGNCC 1 cut(s) 569
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 1 cut(s) 23
RsaI GTAC 1 cut(s) 972
RsaNI GTAC 1 cut(s) 971
SaqAI TTAA 8 cut(s) 72, 165, 276, 540, 630, 815, 855, 1014
SatI GCNGC 7 cut(s) 19, 22, 25, 128, 427, 489, 962
Sau3AI GATC 5 cut(s) 250, 381, 398, 685, 1090
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 3 cut(s) 50, 118, 789
ScrFI CCNGG 3 cut(s) 623, 810, 1010
SduI GDGCHC 2 cut(s) 360, 702
SexAI ACCWGGT 2 cut(s) 621, 1008
SfaNI GCATC 3 cut(s) 138, 256, 471
SfcI CTRYAG 2 cut(s) 19, 180
SfuI TTCGAA 2 cut(s) 159, 549
SinI GGWCC 1 cut(s) 569
SpeI ACTAGT 1 cut(s) 794
SphI GCATGC 1 cut(s) 488
Sse9I AATT 5 cut(s) 133, 162, 714, 799, 1020
SsiI CCGC 3 cut(s) 59, 659, 1027
SspMI CTAG 1 cut(s) 795
StyD4I CCNGG 3 cut(s) 621, 808, 1008
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 97, 389
TaiI ACGT 2 cut(s) 591, 638
TaqI TCGA 3 cut(s) 159, 549, 1093
TasI AATT 5 cut(s) 133, 162, 714, 799, 1020
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 8 cut(s) 72, 165, 276, 540, 630, 815, 855, 1014
Tru9I TTAA 8 cut(s) 72, 165, 276, 540, 630, 815, 855, 1014
TscAI CASTG 2 cut(s) 427, 498
TseFI GTSAC 1 cut(s) 195
TseI GCWGC 7 cut(s) 18, 21, 24, 127, 426, 488, 961
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 2 cut(s) 440, 999
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 2 cut(s) 427, 498
VpaK11BI GGWCC 1 cut(s) 569
XceI RCATGY 1 cut(s) 488
XcmI CCANNNNNNNNNTGG 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 795
Zsp2I ATGCAT 2 cut(s) 271, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.