Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004684

Physical Location & Seq

Genomic Coordinates Forward (+)

20938807 .. 20942379

Gene Structure 3573 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1207300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGCCCAAGTGAGCAAAATCTGCAGCAATGGAGCTCAAAGCATCAATCTTTTGCCCAATGTTTCCAAACCCCAAATGCCCAGATCATCAAATTTTCTCCCATTGAAGTCCCAGTTTCTGGGTTCCTCAAATTCTTTGAGTTTGAAGCTGAAAAATGGGCTTGTGGGCAGTTGGACCGTCGGTAAAGTCAGGGTCGATCCGCTTACAGTTGCAGCTTCAGTTGCCACAGCAGAGAAGCCGTCCACGGTTCCGGAGATTGTGCTGCAACCCATCCAAGAAATCTCGGGCACCATAAAGTTGCCGGGTTCCAAGTCGTTGTCGAATCGAATTCTGCTGATTGCTGCTCTCTCTGAGGGAACAACTGTTGTTGACAACTTGTTAGATAGTGAAGATATTCATTATATGCTTGGTGCATTGAAAACCCTTGGGCTGAATGTTGAAGAGGACAAGGAAAACAGGCGAGCGGTCGTGGAGGGTTGTGGTGGTCGGTTTCCTTTGAGTAATGAATCAGTAGATGAAGTGCAACTATTCCTTGGAAATGCTGGAACAGCAATGCGGCCATTGACTGCTGCAGTTGTTGCTGCTGGTGGACATGCTAGGTATGTACTTGATGGGGTGCCCCGAATGAGGGAGAGACCAATCGGAGACTTAGTTGATGGTCTTAAGCAGCTTGGTGCGGATGCTGATTGTTTTCTTGGAACAAACTGCCCTCCTGTCCGTGTGATTGGAAAGGGAGGCCTTCCAGGAGGGAAGGTGAAGCTCTCTGGATCAATTAGTAGTCAGTACTTGACTGCTTTGCTTATGGCAGCTCCTTTGGCCCTTGGAGATGTTGAAATAGAGATTATTGATAAACTAATTTCCATTCCGTATGTGGAAATGACTTTGAAGTTGATGGAACGCTTTGGGGTCTCAGTGGAACGCAGTGATAGTTGGGATCGGTTTTTGATCCAAGGAGGTCAAAAGTACAAGTCTCCTGGAAATGCTTTTGTCGAAGGCGATGCTTCAAGTGCTAGTTACTTTCTAGCTGGTGCTGCTGTCACTGGTGGGACTGTCACTGTTGAAGGCTGTGGGACAAGCAGTTTACAGGGAGATGTAAAGTTCGCTGAAGTTCTTGAAAAGATGGGTGCTAAAGTTACATGGACAGAGAATTCTGTCACAGTTACAGGACCTCAACGACTTTCTTCTGGAGGAAAACACTTGAAAGCTGTTGACGTCAACATGAACAAAATGCCAGATGTTGCCATGACTCTTGCTGTAGTTGCTCTTTTTGCCGATGGACAAACTGCCATAAGAGATGTGGCAAGTTGGAGAGTGAAGGAGACAGAAAGGATGATCGCCATATGCACTGAACTAAGAAAGCTGGGAGCAACCGTTGAAGAGGGACCAGATTACTGCATAATCACACCGCCAGAAAAATTAAACTTGACAGCAATAGACACGTATGATGACCACCGAATGGCCATGGCTTTCTCTCTTGCTGCCTGTGGAGACGTTCCAGTTACTATCAAGGATCCCGGTTGTACCAGAAAAACATTCCCCGATTACTTTGAAGTCCTCAGGAAGTTTACCAAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003866 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

EPSP_synthase

Protein Analysis

526

Amino Acids

55.96

Weight (kDa)

6.3

Isoelectric Point (pI)

31.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EPSP_synthase	PF00275	89 - 519	2.3e-140	EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48860 AT1G48860 AT2G45300 AT2G45300 AT2G45300 AT2G45300
fragaria_vesca	FvH4_7g11530 FvH4_7g11530
malus_domestica	MD02G1207300.v1.1 MD02G1207500.v1.1 MD07G1119600.v1.1 MD10G1038600.v1.1 MD14G1236200.v1.1
prunus_persica	Prupe.2G143700_v2.0.a1
pyrus_communis	pycom02g16870 pycom04g13590 pycom06g15290 pycom07g10510
rosa_chinensis	RchiOBHm_Chr1g0349971
rosa_laevigata	RLG00000028512
rosa_multiflora	Rmu_sc0000778.1_g000001 Rmu_sc0005220.1_g000012 Rmu_sc0039385.1_g000001
rosa_roxburghii	Rroxscaffold_4G00305240
rosa_rugosa	Rorug01G0207400 Rorug01G0207500
rosa_samantha	Rh1AG224000 Rh1BG191200 Rh1CG208700 Rh1DG219800
rosa_wichuraiana	Rw0G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1215
AccB1I	GGYRCC	2 cut(s)	287, 616
AccB7I	CCANNNNNTGG	2 cut(s)	118, 1456
AccBSI	CCGCTC	1 cut(s)	464
AccIII	TCCGGA	1 cut(s)	250
AciI	CCGC	5 cut(s)	200, 464, 556, 677, 1406
AclWI	GGATC	6 cut(s)	191, 775, 940, 942, 1505, 1518
AcoI	YGGCCR	2 cut(s)	557, 1458
AcsI	RAATTY	4 cut(s)	91, 130, 327, 1147
AcuI	CTGAAG	2 cut(s)	201, 1125
AcyI	GRCGYC	1 cut(s)	1212
AfaI	GTAC	4 cut(s)	606, 785, 965, 1522
AfiI	CCNNNNNNNGG	4 cut(s)	118, 627, 628, 1456
AflII	CTTAAG	1 cut(s)	662
AflIII	ACRYGT	1 cut(s)	1437
AjnI	CCWGG	2 cut(s)	742, 973
AluBI	AGCT	9 cut(s)	35, 148, 215, 670, 760, 809, 1025, 1205, 1360
AluI	AGCT	9 cut(s)	35, 148, 215, 670, 760, 809, 1025, 1205, 1360
Alw21I	GWGCWC	1 cut(s)	37
Alw26I	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
AlwI	GGATC	6 cut(s)	191, 775, 940, 942, 1505, 1518
AlwNI	CAGNNNCTG	1 cut(s)	118
Ama87I	CYCGRG	1 cut(s)	283
Aor13HI	TCCGGA	1 cut(s)	250
AoxI	GGCC	5 cut(s)	3, 557, 736, 816, 1458
ApoI	RAATTY	4 cut(s)	91, 130, 327, 1147
Asp700I	GAANNNNTTC	1 cut(s)	393
AspS9I	GGNCC	5 cut(s)	4, 174, 817, 1166, 1382
AsuC2I	CCSGG	2 cut(s)	303, 1515
AsuHPI	GGTGA	1 cut(s)	766
AvaI	CYCGRG	1 cut(s)	283
AvaII	GGWCC	3 cut(s)	174, 1166, 1382
AxyI	CCTNAGG	1 cut(s)	1556
BaeGI	GKGCMC	2 cut(s)	290, 621
BalI	TGGCCA	1 cut(s)	1460
BamHI	GGATCC	1 cut(s)	1510
BanI	GGYRCC	2 cut(s)	287, 616
BanII	GRGCYC	1 cut(s)	37
Bbv12I	GWGCWC	1 cut(s)	37
BccI	CCATC	6 cut(s)	278, 605, 650, 886, 1114, 1268
BceAI	ACGGC	1 cut(s)	223
BcgI	CGANNNNNNTGC	2 cut(s)	980, 1014
BciT130I	CCWGG	2 cut(s)	744, 975
BcnI	CCSGG	2 cut(s)	303, 1515
BcoDI	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
BfaI	CTAG	3 cut(s)	597, 1011, 1022
BfmI	CTRYAG	3 cut(s)	22, 570, 1254
BfrI	CTTAAG	1 cut(s)	662
BmcAI	AGTACT	1 cut(s)	785
Bme1390I	CCNGG	4 cut(s)	303, 744, 975, 1515
Bme18I	GGWCC	3 cut(s)	174, 1166, 1382
BmeT110I	CYCGRG	1 cut(s)	283
BmgT120I	GGNCC	5 cut(s)	4, 174, 817, 1166, 1382
BmiI	GGNNCC	7 cut(s)	124, 249, 289, 307, 618, 1383, 1512
BmrFI	CCNGG	4 cut(s)	303, 744, 975, 1515
BmrI	ACTGGG	1 cut(s)	106
BmsI	GCATC	3 cut(s)	51, 670, 988
BmuI	ACTGGG	1 cut(s)	106
BpmI	CTGGAG	1 cut(s)	1206
BpuMI	CCSGG	2 cut(s)	303, 1515
BsaAI	YACGTR	1 cut(s)	1440
BsaHI	GRCGYC	1 cut(s)	1212
BsaI	GGTCTC	2 cut(s)	628, 913
BsaJI	CCNNGG	6 cut(s)	243, 424, 532, 820, 949, 1461
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	6 cut(s)	636, 666, 738, 768, 1356, 1386
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 627, 628, 1456
Bse1I	ACTGG	3 cut(s)	112, 1045, 1496
Bse21I	CCTNAGG	1 cut(s)	1556
Bse3DI	GCAATG	2 cut(s)	34, 558
BseAI	TCCGGA	1 cut(s)	250
BseBI	CCWGG	2 cut(s)	744, 975
BseDI	CCNNGG	6 cut(s)	243, 424, 532, 820, 949, 1461
BseGI	GGATG	3 cut(s)	270, 685, 1335
BseLI	CCNNNNNNNGG	4 cut(s)	118, 627, 628, 1456
BseMI	GCAATG	2 cut(s)	34, 558
BseMII	CTCAG	3 cut(s)	342, 924, 1570
BseNI	ACTGG	3 cut(s)	112, 1045, 1496
BseSI	GKGCMC	2 cut(s)	290, 621
BseYI	CCCAGC	1 cut(s)	1360
Bsh1285I	CGRYCG	1 cut(s)	468
BshFI	GGCC	5 cut(s)	5, 559, 738, 818, 1460
BshNI	GGYRCC	2 cut(s)	287, 616
BsiEI	CGRYCG	1 cut(s)	468
BsiHKAI	GWGCWC	1 cut(s)	37
BsiHKCI	CYCGRG	1 cut(s)	283
BsiSI	CCGG	3 cut(s)	251, 302, 1515
BslFI	GGGAC	4 cut(s)	94, 1060, 1084, 1395
BslI	CCNNNNNNNGG	4 cut(s)	118, 627, 628, 1456
BsmAI	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
BsmBI	CGTCTC	1 cut(s)	1482
BsmFI	GGGAC	4 cut(s)	94, 1060, 1084, 1395
BsnI	GGCC	5 cut(s)	5, 559, 738, 818, 1460
Bso31I	GGTCTC	2 cut(s)	628, 913
BsoBI	CYCGRG	1 cut(s)	283
Bsp1286I	GDGCHC	3 cut(s)	37, 290, 621
Bsp13I	TCCGGA	1 cut(s)	250
Bsp143I	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
Bsp19I	CCATGG	1 cut(s)	1461
BspACI	CCGC	5 cut(s)	200, 464, 556, 677, 1406
BspANI	GGCC	5 cut(s)	5, 559, 738, 818, 1460
BspCNI	CTCAG	3 cut(s)	343, 923, 1569
BspEI	TCCGGA	1 cut(s)	250
BspLI	GGNNCC	7 cut(s)	124, 249, 289, 307, 618, 1383, 1512
BspMAI	CTGCAG	2 cut(s)	26, 574
BspPI	GGATC	6 cut(s)	191, 775, 940, 942, 1505, 1518
BspT107I	GGYRCC	2 cut(s)	287, 616
BspTI	CTTAAG	1 cut(s)	662
BspTNI	GGTCTC	2 cut(s)	628, 913
BsrBI	CCGCTC	1 cut(s)	464
BsrDI	GCAATG	2 cut(s)	34, 558
BsrI	ACTGG	3 cut(s)	112, 1045, 1496
BssECI	CCNNGG	6 cut(s)	243, 424, 532, 820, 949, 1461
BssMI	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
BssNI	GRCGYC	1 cut(s)	1212
BssT1I	CCWWGG	5 cut(s)	424, 532, 820, 949, 1461
Bst2UI	CCWGG	2 cut(s)	744, 975
Bst4CI	ACNGT	8 cut(s)	178, 208, 247, 364, 1051, 1057, 1159, 1372
Bst6I	CTCTTC	2 cut(s)	435, 1371
BstACI	GRCGYC	1 cut(s)	1212
BstAFI	CTTAAG	1 cut(s)	662
BstAPI	GCANNNNNTGC	2 cut(s)	21, 578
BstBAI	YACGTR	1 cut(s)	1440
BstC8I	GCNNGC	1 cut(s)	462
BstDEI	CTNAG	5 cut(s)	351, 649, 910, 1352, 1556
BstDSI	CCRYGG	2 cut(s)	243, 1461
BstF5I	GGATG	3 cut(s)	270, 685, 1335
BstKTI	GATC	7 cut(s)	86, 199, 770, 937, 948, 1335, 1513
BstMAI	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
BstMBI	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
BstMCI	CGRYCG	1 cut(s)	468
BstMWI	GCNNNNNNNGC	9 cut(s)	21, 221, 548, 578, 815, 1007, 1031, 1259, 1268
BstNI	CCWGG	2 cut(s)	744, 975
BstNSI	RCATGY	1 cut(s)	596
BstSCI	CCNGG	4 cut(s)	301, 742, 973, 1513
BstSFI	CTRYAG	3 cut(s)	22, 570, 1254
BstSLI	GKGCMC	2 cut(s)	290, 621
BstX2I	RGATCY	1 cut(s)	1510
BstYI	RGATCY	1 cut(s)	1510
Bsu36I	CCTNAGG	1 cut(s)	1556
BsuRI	GGCC	5 cut(s)	5, 559, 738, 818, 1460
BtgI	CCRYGG	2 cut(s)	243, 1461
BtgZI	GCGATG	1 cut(s)	1011
BtsCI	GGATG	3 cut(s)	270, 685, 1335
BtsI	GCAGTG	1 cut(s)	928
BtsIMutI	CAGTG	5 cut(s)	918, 928, 1038, 1053, 1344
Cac8I	GCNNGC	1 cut(s)	462
CaiI	CAGNNNCTG	1 cut(s)	118
Cfr13I	GGNCC	5 cut(s)	4, 174, 817, 1166, 1382
Csp6I	GTAC	4 cut(s)	605, 784, 964, 1521
CviAII	CATG	5 cut(s)	593, 1137, 1219, 1243, 1462
CviQI	GTAC	4 cut(s)	605, 784, 964, 1521
DdeI	CTNAG	5 cut(s)	351, 649, 910, 1352, 1556
DpnI	GATC	7 cut(s)	85, 198, 769, 936, 947, 1334, 1512
DpnII	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
EaeI	YGGCCR	2 cut(s)	557, 1458
Eam1104I	CTCTTC	2 cut(s)	435, 1371
EarI	CTCTTC	2 cut(s)	435, 1371
Ecl136II	GAGCTC	1 cut(s)	35
Eco130I	CCWWGG	5 cut(s)	424, 532, 820, 949, 1461
Eco147I	AGGCCT	1 cut(s)	738
Eco24I	GRGCYC	1 cut(s)	37
Eco31I	GGTCTC	2 cut(s)	628, 913
Eco47I	GGWCC	3 cut(s)	174, 1166, 1382
Eco53kI	GAGCTC	1 cut(s)	35
Eco57I	CTGAAG	2 cut(s)	201, 1125
Eco81I	CCTNAGG	1 cut(s)	1556
Eco88I	CYCGRG	1 cut(s)	283
EcoICRI	GAGCTC	1 cut(s)	35
EcoO109I	RGGNCCY	1 cut(s)	1166
EcoRI	GAATTC	2 cut(s)	327, 1147
EcoRII	CCWGG	2 cut(s)	742, 973
EcoT14I	CCWWGG	5 cut(s)	424, 532, 820, 949, 1461
EcoT38I	GRGCYC	1 cut(s)	37
ErhI	CCWWGG	5 cut(s)	424, 532, 820, 949, 1461
Esp3I	CGTCTC	1 cut(s)	1482
FaeI	CATG	5 cut(s)	596, 1140, 1222, 1246, 1465
FaqI	GGGAC	4 cut(s)	94, 1060, 1084, 1395
FatI	CATG	5 cut(s)	592, 1136, 1218, 1242, 1461
FauNDI	CATATG	1 cut(s)	1340
FokI	GGATG	3 cut(s)	257, 692, 1342
FriOI	GRGCYC	1 cut(s)	37
FspBI	CTAG	3 cut(s)	597, 1011, 1022
GsaI	CCCAGC	1 cut(s)	1364
GsuI	CTGGAG	1 cut(s)	1206
HaeIII	GGCC	5 cut(s)	5, 559, 738, 818, 1460
HapII	CCGG	3 cut(s)	251, 302, 1515
Hin1I	GRCGYC	1 cut(s)	1212
Hin1II	CATG	5 cut(s)	596, 1140, 1222, 1246, 1465
HincII	GTYRAC	3 cut(s)	370, 1210, 1216
HindII	GTYRAC	3 cut(s)	370, 1210, 1216
HinfI	GANTC	3 cut(s)	322, 506, 1246
HpaII	CCGG	3 cut(s)	251, 302, 1515
HphI	GGTGA	1 cut(s)	766
Hpy166II	GTNNAC	7 cut(s)	243, 370, 590, 1082, 1210, 1216, 1566
Hpy188I	TCNGA	2 cut(s)	352, 644
Hpy188III	TCNNGA	5 cut(s)	251, 765, 1112, 1185, 1558
Hpy8I	GTNNAC	7 cut(s)	243, 370, 590, 1082, 1210, 1216, 1566
Hpy99I	CGWCG	1 cut(s)	182
HpyAV	CCTTC	5 cut(s)	745, 749, 986, 1055, 1309
HpyCH4III	ACNGT	8 cut(s)	178, 208, 247, 364, 1051, 1057, 1159, 1372
HpyCH4IV	ACGT	3 cut(s)	1212, 1439, 1491
HpyCH4V	TGCA	8 cut(s)	24, 212, 265, 413, 523, 572, 1344, 1395
HpyF10VI	GCNNNNNNNGC	9 cut(s)	21, 221, 548, 578, 815, 1007, 1031, 1259, 1268
HpyF3I	CTNAG	5 cut(s)	351, 649, 910, 1352, 1556
HpySE526I	ACGT	3 cut(s)	1212, 1439, 1491
Hsp92I	GRCGYC	1 cut(s)	1212
Hsp92II	CATG	5 cut(s)	596, 1140, 1222, 1246, 1465
Kpn2I	TCCGGA	1 cut(s)	250
Kzo9I	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
LmnI	GCTCC	3 cut(s)	32, 814, 1364
LweI	GCATC	3 cut(s)	51, 670, 988
MaeI	CTAG	3 cut(s)	597, 1011, 1022
MaeII	ACGT	3 cut(s)	1212, 1439, 1491
MaeIII	GTNAC	7 cut(s)	1013, 1036, 1051, 1132, 1153, 1159, 1498
MalI	GATC	7 cut(s)	85, 198, 769, 936, 947, 1334, 1512
MbiI	CCGCTC	1 cut(s)	464
MboI	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
MboII	GAAGA	4 cut(s)	401, 452, 1173, 1388
MflI	RGATCY	1 cut(s)	1510
MhlI	GDGCHC	3 cut(s)	37, 290, 621
MlsI	TGGCCA	1 cut(s)	1460
MluCI	AATT	7 cut(s)	91, 130, 327, 771, 855, 1147, 1415
MluNI	TGGCCA	1 cut(s)	1460
MlyI	GAGTC	1 cut(s)	1240
MmeI	TCCRAC	2 cut(s)	152, 1286
Mox20I	TGGCCA	1 cut(s)	1460
MroI	TCCGGA	1 cut(s)	250
MroXI	GAANNNNTTC	1 cut(s)	393
MscI	TGGCCA	1 cut(s)	1460
MseI	TTAA	2 cut(s)	663, 1418
Msp20I	TGGCCA	1 cut(s)	1460
MspCI	CTTAAG	1 cut(s)	662
MspI	CCGG	3 cut(s)	251, 302, 1515
MspR9I	CCNGG	4 cut(s)	303, 744, 975, 1515
MvaI	CCWGG	2 cut(s)	744, 975
MwoI	GCNNNNNNNGC	9 cut(s)	21, 221, 548, 578, 815, 1007, 1031, 1259, 1268
NciI	CCSGG	2 cut(s)	303, 1515
NcoI	CCATGG	1 cut(s)	1461
NdeI	CATATG	1 cut(s)	1340
NdeII	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
NlaIII	CATG	5 cut(s)	596, 1140, 1222, 1246, 1465
NlaIV	GGNNCC	7 cut(s)	124, 249, 289, 307, 618, 1383, 1512
NmuCI	GTSAC	3 cut(s)	1036, 1051, 1153
NspI	RCATGY	1 cut(s)	596
PceI	AGGCCT	1 cut(s)	738
PdmI	GAANNNNTTC	1 cut(s)	393
PfeI	GAWTC	2 cut(s)	322, 506
PflMI	CCANNNNNTGG	2 cut(s)	118, 1456
PfoI	TCCNGGA	2 cut(s)	742, 973
PleI	GAGTC	1 cut(s)	1240
PpsI	GAGTC	1 cut(s)	1240
Ppu21I	YACGTR	1 cut(s)	1440
PpuMI	RGGWCCY	1 cut(s)	1166
Psp124BI	GAGCTC	1 cut(s)	37
Psp5II	RGGWCCY	1 cut(s)	1166
Psp6I	CCWGG	2 cut(s)	742, 973
PspFI	CCCAGC	1 cut(s)	1360
PspGI	CCWGG	2 cut(s)	742, 973
PspN4I	GGNNCC	7 cut(s)	124, 249, 289, 307, 618, 1383, 1512
PspPI	GGNCC	5 cut(s)	4, 174, 817, 1166, 1382
PspPPI	RGGWCCY	1 cut(s)	1166
PstI	CTGCAG	2 cut(s)	26, 574
PstNI	CAGNNNCTG	1 cut(s)	118
PsuI	RGATCY	1 cut(s)	1510
RsaI	GTAC	4 cut(s)	606, 785, 965, 1522
RsaNI	GTAC	4 cut(s)	605, 784, 964, 1521
SacI	GAGCTC	1 cut(s)	37
SaqAI	TTAA	2 cut(s)	663, 1418
Sau3AI	GATC	7 cut(s)	83, 196, 767, 934, 945, 1332, 1510
Sau96I	GGNCC	5 cut(s)	4, 174, 817, 1166, 1382
ScaI	AGTACT	1 cut(s)	785
SchI	GAGTC	1 cut(s)	1240
ScrFI	CCNGG	4 cut(s)	303, 744, 975, 1515
SduI	GDGCHC	3 cut(s)	37, 290, 621
SfaNI	GCATC	3 cut(s)	51, 670, 988
SfcI	CTRYAG	3 cut(s)	22, 570, 1254
SinI	GGWCC	3 cut(s)	174, 1166, 1382
SmlI	CTYRAG	1 cut(s)	662
SmoI	CTYRAG	1 cut(s)	662
Sse9I	AATT	7 cut(s)	91, 130, 327, 771, 855, 1147, 1415
SseBI	AGGCCT	1 cut(s)	738
SsiI	CCGC	5 cut(s)	200, 464, 556, 677, 1406
SspMI	CTAG	3 cut(s)	597, 1011, 1022
SstI	GAGCTC	1 cut(s)	37
StuI	AGGCCT	1 cut(s)	738
StyD4I	CCNGG	4 cut(s)	301, 742, 973, 1513
StyI	CCWWGG	5 cut(s)	424, 532, 820, 949, 1461
TaaI	ACNGT	8 cut(s)	178, 208, 247, 364, 1051, 1057, 1159, 1372
TaiI	ACGT	3 cut(s)	1215, 1442, 1494
TaqI	TCGA	4 cut(s)	195, 320, 325, 990
TasI	AATT	7 cut(s)	91, 130, 327, 771, 855, 1147, 1415
TatI	WGTACW	3 cut(s)	604, 783, 963
TauI	GCSGC	1 cut(s)	559
TfiI	GAWTC	2 cut(s)	322, 506
Tru1I	TTAA	2 cut(s)	663, 1418
Tru9I	TTAA	2 cut(s)	663, 1418
TscAI	CASTG	5 cut(s)	918, 928, 1045, 1060, 1351
TseFI	GTSAC	3 cut(s)	1036, 1051, 1153
Tsp45I	GTSAC	3 cut(s)	1036, 1051, 1153
TspDTI	ATGAA	4 cut(s)	386, 519, 531, 1235
TspGWI	ACGGA	2 cut(s)	707, 855
TspRI	CASTG	5 cut(s)	918, 928, 1045, 1060, 1351
Van91I	CCANNNNNTGG	2 cut(s)	118, 1456
Vha464I	CTTAAG	1 cut(s)	662
VpaK11BI	GGWCC	3 cut(s)	174, 1166, 1382
XapI	RAATTY	4 cut(s)	91, 130, 327, 1147
XceI	RCATGY	1 cut(s)	596
XcmI	CCANNNNNNNNNTGG	2 cut(s)	868, 1294
XmnI	GAANNNNTTC	1 cut(s)	393
XspI	CTAG	3 cut(s)	597, 1011, 1022
ZraI	GACGTC	1 cut(s)	1213
ZrmI	AGTACT	1 cut(s)	785

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.