Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004684

Physical Location & Seq

Genomic Coordinates Forward (+)

14949837 .. 14953257

Gene Structure 3421 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1119600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGCCCAGGTGAGCAAAATCTGCAGTAATGGAGCTCAAAGCATCAACCTTTTTCCCAATGTTTCCAAACCCCAAATACCCAAATCCTCAAATTTGCACCCATTGAAGTCACATTTTCTGGGTTCCTCAAATTCTTTGAGTTTGAAGCTGAAATATGGGTTTGTTGGCAGTTTTACGGTCGGTAAATTCAGGGTCGATCCCCTTAGAATTGCAGCTTCAGTTGCCACGGCAGAGAAGCCGTCGACAGTTCCGGAGATTGTGCTGCAACCCATCGAAGAAATCTCCGGCACCATAAAGTTGCCGGGTTCCAAGTCGTTGTCGAATCGGATTCTGCTTATTGCTGCTCTCTCTGAGGGAACAACTGTTGTTGACAACTTATTAGATAGTGATGATATTCATTATATGCTTGGTGCATTGAAAACCCTTGGGCTGAATGTTGAAGAGGACAAGGTAAACAAGCGAGCAGTCGTGGAGGGTTGTGGTGGTCGGTTTCCTTTGAGTAATGAATCAGTAGATGAAGTTCAACTATTCCTTGGAAATGCTGGAACAGCAATGCGGCCATTGACTGCTGCAATTGTTGCTGCTGGTGGACATGCTAGGTATGTACTTGATGGGGTGCCTCGAATGAGGGAGAGACCAATCGGAGACTTGGTTGATGGTCTTAAGCAGCTTGGTGCAGATGCTGATTGTTTTCTTGGAACAAACTGCCCTCCTGTCCGTGTGATTGGAAAGGGAGGCCTTCCAGGGGGGAAGGTGAAGCTCTCTGGATCAATTAGTAGTCAGTACTTGACTGCTTTGCTTATGGCAGCTCCTTTGGCTCTTGGAGATGTTGAGATCGATATCATTGATAAACTAATTTCCATTCCGTATGTGGAAATGACTTTGAAGTTGATGGAATGCTTTGGGGTCTCAGTGGAACACAGTGATAGCTGGGATCGGTTTTTGATCCGAGGAGGTCAAAAGTACAAGTCTCCTGGAAATGCATTTGTCGAAGGTGATGCTTCAAGTGCTAGTTACTTTCTAGCTGGTGCTGCTGTCACTGGTGGGACTGTCACTGTTGAAGGCTGTGGGACAAGCAGTTTACAGGGAGATGTAAAGTTTGCTGAAGTTCTTGAAAAGATGGGTGCTAAAGTTACCTGGACAGAGAATTCTGTTACAGTTACAGGACCTCAACGACTTTCTGCCGGAGGCAAACACTTGAAAGCTGTTGATGTTAACATGAACAAAATGCCAGATGTTGCCATGACTCTTGCCGTAGTTGCTCTTTTTGCCGATGGCCCAACTGCCATAAGAGATGTGGCAAGCTGGAGAGTGAAGGAGACGGAAAGGATGATTGCCATTTGCACTGAACTAAGAAAGCTGGGAGCAACTGTTGAAGAAGGACCAGATTACTGCATAATCACACCGCCAGAAAAATTAAACGTGACAGCAATAGACACGTATGATGACCACCGAATGGCCATGGCTTTCTCTCTTGCTGCCTGTGGAGACGTTCCAGTTACTATCAAGGATCCTGGTTGTACACGAAAAACATTCCCCGATTACTTTGAAGTCCTTGGGAGGTTCACCAAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003866 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

EPSP_synthase

Protein Analysis

526

Amino Acids

55.86

Weight (kDa)

6.29

Isoelectric Point (pI)

28.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EPSP_synthase	PF00275	89 - 519	3.4e-139	EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48860 AT1G48860 AT2G45300 AT2G45300 AT2G45300 AT2G45300
fragaria_vesca	FvH4_7g11530 FvH4_7g11530
malus_domestica	MD02G1207300.v1.1 MD02G1207500.v1.1 MD07G1119600.v1.1 MD10G1038600.v1.1 MD14G1236200.v1.1
prunus_persica	Prupe.2G143700_v2.0.a1
pyrus_communis	pycom02g16870 pycom04g13590 pycom06g15290 pycom07g10510
rosa_chinensis	RchiOBHm_Chr1g0349971
rosa_laevigata	RLG00000028512
rosa_multiflora	Rmu_sc0000778.1_g000001 Rmu_sc0005220.1_g000012 Rmu_sc0039385.1_g000001
rosa_roxburghii	Rroxscaffold_4G00305240
rosa_rugosa	Rorug01G0207400 Rorug01G0207500
rosa_samantha	Rh1AG224000 Rh1BG191200 Rh1CG208700 Rh1DG219800
rosa_wichuraiana	Rw0G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	287, 616
AccB7I	CCANNNNNTGG	1 cut(s)	1456
AccI	GTMKAC	1 cut(s)	242
AccIII	TCCGGA	1 cut(s)	250
AciI	CCGC	2 cut(s)	556, 1406
AclWI	GGATC	6 cut(s)	191, 775, 940, 942, 1505, 1518
AcoI	YGGCCR	2 cut(s)	557, 1458
AcsI	RAATTY	4 cut(s)	91, 130, 185, 1147
AcuI	CTGAAG	2 cut(s)	201, 1125
AfaI	GTAC	4 cut(s)	606, 785, 965, 1522
AfiI	CCNNNNNNNGG	2 cut(s)	745, 1456
AflII	CTTAAG	1 cut(s)	662
AflIII	ACRYGT	1 cut(s)	1437
AjnI	CCWGG	5 cut(s)	6, 742, 973, 1136, 1513
Alw21I	GWGCWC	1 cut(s)	37
Alw26I	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
AlwI	GGATC	6 cut(s)	191, 775, 940, 942, 1505, 1518
AlwNI	CAGNNNCTG	1 cut(s)	683
Aor13HI	TCCGGA	1 cut(s)	250
AoxI	GGCC	5 cut(s)	3, 557, 736, 1276, 1458
ApeKI	GCWGC	9 cut(s)	212, 262, 341, 569, 581, 667, 806, 1031, 1478
ApoI	RAATTY	4 cut(s)	91, 130, 185, 1147
AspS9I	GGNCC	4 cut(s)	4, 1166, 1277, 1382
AsuC2I	CCSGG	1 cut(s)	303
AsuHPI	GGTGA	4 cut(s)	22, 766, 1007, 1558
AvaII	GGWCC	2 cut(s)	1166, 1382
BalI	TGGCCA	1 cut(s)	1460
BamHI	GGATCC	1 cut(s)	1510
BanI	GGYRCC	2 cut(s)	287, 616
BanII	GRGCYC	1 cut(s)	37
Bbv12I	GWGCWC	1 cut(s)	37
BbvI	GCAGC	9 cut(s)	224, 249, 328, 556, 568, 679, 818, 1018, 1465
BccI	CCATC	6 cut(s)	278, 605, 650, 886, 1114, 1268
BceAI	ACGGC	3 cut(s)	223, 243, 1238
BcgI	CGANNNNNNTGC	2 cut(s)	980, 1014
BciT130I	CCWGG	5 cut(s)	8, 744, 975, 1138, 1515
BcnI	CCSGG	1 cut(s)	303
BcoDI	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
BfaI	CTAG	3 cut(s)	597, 1011, 1022
BfmI	CTRYAG	1 cut(s)	22
BfrI	CTTAAG	1 cut(s)	662
BmcAI	AGTACT	1 cut(s)	785
Bme1390I	CCNGG	6 cut(s)	8, 303, 744, 975, 1138, 1515
Bme18I	GGWCC	2 cut(s)	1166, 1382
BmgT120I	GGNCC	4 cut(s)	4, 1166, 1277, 1382
BmiI	GGNNCC	5 cut(s)	124, 289, 307, 618, 1512
BmrFI	CCNGG	6 cut(s)	8, 303, 744, 975, 1138, 1515
BmsI	GCATC	3 cut(s)	51, 670, 988
BpmI	CTGGAG	1 cut(s)	1327
BpuMI	CCSGG	1 cut(s)	303
Bsa29I	ATCGAT	1 cut(s)	837
BsaAI	YACGTR	1 cut(s)	1440
BsaBI	GATNNNNATC	1 cut(s)	839
BsaI	GGTCTC	2 cut(s)	628, 913
BsaJI	CCNNGG	8 cut(s)	6, 225, 424, 532, 743, 949, 1461, 1555
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	4 cut(s)	636, 666, 1356, 1386
Bsc4I	CCNNNNNNNGG	2 cut(s)	745, 1456
Bse1I	ACTGG	2 cut(s)	1045, 1496
Bse3DI	GCAATG	1 cut(s)	558
Bse8I	GATNNNNATC	1 cut(s)	839
BseAI	TCCGGA	1 cut(s)	250
BseBI	CCWGG	5 cut(s)	8, 744, 975, 1138, 1515
BseCI	ATCGAT	1 cut(s)	837
BseDI	CCNNGG	8 cut(s)	6, 225, 424, 532, 743, 949, 1461, 1555
BseGI	GGATG	1 cut(s)	1335
BseJI	GATNNNNATC	1 cut(s)	839
BseLI	CCNNNNNNNGG	2 cut(s)	745, 1456
BseMI	GCAATG	1 cut(s)	558
BseMII	CTCAG	2 cut(s)	342, 924
BseNI	ACTGG	2 cut(s)	1045, 1496
BseRI	GAGGAG	1 cut(s)	966
BseXI	GCAGC	9 cut(s)	224, 249, 328, 556, 568, 679, 818, 1018, 1465
BseYI	CCCAGC	2 cut(s)	930, 1360
BsgI	GTGCAG	1 cut(s)	696
Bsh1285I	CGRYCG	1 cut(s)	180
BshFI	GGCC	5 cut(s)	5, 559, 738, 1278, 1460
BshNI	GGYRCC	2 cut(s)	287, 616
BshVI	ATCGAT	1 cut(s)	837
BsiEI	CGRYCG	1 cut(s)	180
BsiHKAI	GWGCWC	1 cut(s)	37
BsiSI	CCGG	4 cut(s)	251, 285, 302, 1185
BslFI	GGGAC	2 cut(s)	1060, 1084
BslI	CCNNNNNNNGG	2 cut(s)	745, 1456
BsmAI	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
BsmBI	CGTCTC	2 cut(s)	1313, 1482
BsmFI	GGGAC	2 cut(s)	1060, 1084
BsmI	GAATGC	1 cut(s)	902
BsnI	GGCC	5 cut(s)	5, 559, 738, 1278, 1460
Bso31I	GGTCTC	2 cut(s)	628, 913
Bsp1286I	GDGCHC	1 cut(s)	37
Bsp13I	TCCGGA	1 cut(s)	250
Bsp1407I	TGTACA	1 cut(s)	1520
Bsp143I	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
Bsp19I	CCATGG	1 cut(s)	1461
BspACI	CCGC	2 cut(s)	556, 1406
BspANI	GGCC	5 cut(s)	5, 559, 738, 1278, 1460
BspCNI	CTCAG	2 cut(s)	343, 923
BspDI	ATCGAT	1 cut(s)	837
BspEI	TCCGGA	1 cut(s)	250
BspLI	GGNNCC	5 cut(s)	124, 289, 307, 618, 1512
BspMAI	CTGCAG	1 cut(s)	26
BspPI	GGATC	6 cut(s)	191, 775, 940, 942, 1505, 1518
BspT107I	GGYRCC	2 cut(s)	287, 616
BspTI	CTTAAG	1 cut(s)	662
BspTNI	GGTCTC	2 cut(s)	628, 913
BsrDI	GCAATG	1 cut(s)	558
BsrGI	TGTACA	1 cut(s)	1520
BsrI	ACTGG	2 cut(s)	1045, 1496
BssECI	CCNNGG	8 cut(s)	6, 225, 424, 532, 743, 949, 1461, 1555
BssMI	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
BssT1I	CCWWGG	4 cut(s)	424, 532, 1461, 1555
Bst2UI	CCWGG	5 cut(s)	8, 744, 975, 1138, 1515
Bst4CI	ACNGT	8 cut(s)	178, 247, 364, 923, 1051, 1057, 1159, 1372
Bst6I	CTCTTC	1 cut(s)	435
BstAFI	CTTAAG	1 cut(s)	662
BstAPI	GCANNNNNTGC	2 cut(s)	21, 578
BstAUI	TGTACA	1 cut(s)	1520
BstBAI	YACGTR	1 cut(s)	1440
BstC8I	GCNNGC	2 cut(s)	462, 1303
BstDEI	CTNAG	4 cut(s)	203, 351, 910, 1352
BstDSI	CCRYGG	2 cut(s)	225, 1461
BstF5I	GGATG	1 cut(s)	1335
BstKTI	GATC	6 cut(s)	199, 770, 837, 937, 948, 1513
BstMAI	GTCTC	6 cut(s)	628, 639, 913, 975, 1313, 1482
BstMBI	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
BstMCI	CGRYCG	1 cut(s)	180
BstMWI	GCNNNNNNNGC	9 cut(s)	21, 221, 548, 578, 815, 1007, 1031, 1259, 1268
BstNI	CCWGG	5 cut(s)	8, 744, 975, 1138, 1515
BstNSI	RCATGY	1 cut(s)	596
BstSCI	CCNGG	6 cut(s)	6, 301, 742, 973, 1136, 1513
BstSFI	CTRYAG	1 cut(s)	22
BstV1I	GCAGC	9 cut(s)	224, 249, 328, 556, 568, 679, 818, 1018, 1465
BstX2I	RGATCY	1 cut(s)	1510
BstYI	RGATCY	1 cut(s)	1510
Bsu15I	ATCGAT	1 cut(s)	837
BsuRI	GGCC	5 cut(s)	5, 559, 738, 1278, 1460
BsuTUI	ATCGAT	1 cut(s)	837
BtgI	CCRYGG	2 cut(s)	225, 1461
BtsCI	GGATG	1 cut(s)	1335
BtsIMutI	CAGTG	5 cut(s)	918, 928, 1038, 1053, 1344
Cac8I	GCNNGC	2 cut(s)	462, 1303
CaiI	CAGNNNCTG	1 cut(s)	683
Cfr13I	GGNCC	4 cut(s)	4, 1166, 1277, 1382
ClaI	ATCGAT	1 cut(s)	837
Csp6I	GTAC	4 cut(s)	605, 784, 964, 1521
CviAII	CATG	4 cut(s)	593, 1219, 1243, 1462
CviQI	GTAC	4 cut(s)	605, 784, 964, 1521
DdeI	CTNAG	4 cut(s)	203, 351, 910, 1352
DpnI	GATC	6 cut(s)	198, 769, 836, 936, 947, 1512
DpnII	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
EaeI	YGGCCR	2 cut(s)	557, 1458
Eam1104I	CTCTTC	1 cut(s)	435
EarI	CTCTTC	1 cut(s)	435
Ecl136II	GAGCTC	1 cut(s)	35
Eco130I	CCWWGG	4 cut(s)	424, 532, 1461, 1555
Eco147I	AGGCCT	1 cut(s)	738
Eco24I	GRGCYC	1 cut(s)	37
Eco31I	GGTCTC	2 cut(s)	628, 913
Eco32I	GATATC	1 cut(s)	841
Eco47I	GGWCC	2 cut(s)	1166, 1382
Eco53kI	GAGCTC	1 cut(s)	35
Eco57I	CTGAAG	2 cut(s)	201, 1125
EcoICRI	GAGCTC	1 cut(s)	35
EcoO109I	RGGNCCY	1 cut(s)	1166
EcoRI	GAATTC	1 cut(s)	1147
EcoRII	CCWGG	5 cut(s)	6, 742, 973, 1136, 1513
EcoRV	GATATC	1 cut(s)	841
EcoT14I	CCWWGG	4 cut(s)	424, 532, 1461, 1555
EcoT22I	ATGCAT	1 cut(s)	985
EcoT38I	GRGCYC	1 cut(s)	37
ErhI	CCWWGG	4 cut(s)	424, 532, 1461, 1555
Esp3I	CGTCTC	2 cut(s)	1313, 1482
FaeI	CATG	4 cut(s)	596, 1222, 1246, 1465
FaqI	GGGAC	2 cut(s)	1060, 1084
FatI	CATG	4 cut(s)	592, 1218, 1242, 1461
FblI	GTMKAC	1 cut(s)	242
FokI	GGATG	1 cut(s)	1342
FriOI	GRGCYC	1 cut(s)	37
FspBI	CTAG	3 cut(s)	597, 1011, 1022
GsaI	CCCAGC	2 cut(s)	934, 1364
GsuI	CTGGAG	1 cut(s)	1327
HaeIII	GGCC	5 cut(s)	5, 559, 738, 1278, 1460
HapII	CCGG	4 cut(s)	251, 285, 302, 1185
Hin1II	CATG	4 cut(s)	596, 1222, 1246, 1465
HincII	GTYRAC	3 cut(s)	243, 370, 1216
HindII	GTYRAC	3 cut(s)	243, 370, 1216
HinfI	GANTC	4 cut(s)	322, 328, 506, 1246
HpaI	GTTAAC	1 cut(s)	1216
HpaII	CCGG	4 cut(s)	251, 285, 302, 1185
HphI	GGTGA	4 cut(s)	22, 766, 1007, 1558
Hpy166II	GTNNAC	8 cut(s)	243, 370, 454, 590, 1082, 1216, 1523, 1566
Hpy188I	TCNGA	4 cut(s)	327, 352, 644, 950
Hpy188III	TCNNGA	3 cut(s)	251, 765, 1112
Hpy8I	GTNNAC	8 cut(s)	243, 370, 454, 590, 1082, 1216, 1523, 1566
Hpy99I	CGWCG	1 cut(s)	244
HpyAV	CCTTC	6 cut(s)	745, 749, 986, 1055, 1309, 1373
HpyCH4III	ACNGT	8 cut(s)	178, 247, 364, 923, 1051, 1057, 1159, 1372
HpyCH4IV	ACGT	3 cut(s)	1422, 1439, 1491
HpyF10VI	GCNNNNNNNGC	9 cut(s)	21, 221, 548, 578, 815, 1007, 1031, 1259, 1268
HpyF3I	CTNAG	4 cut(s)	203, 351, 910, 1352
HpySE526I	ACGT	3 cut(s)	1422, 1439, 1491
Hsp92II	CATG	4 cut(s)	596, 1222, 1246, 1465
Kpn2I	TCCGGA	1 cut(s)	250
KspAI	GTTAAC	1 cut(s)	1216
Kzo9I	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
LmnI	GCTCC	3 cut(s)	32, 814, 1364
Lsp1109I	GCAGC	9 cut(s)	224, 249, 328, 556, 568, 679, 818, 1018, 1465
LweI	GCATC	3 cut(s)	51, 670, 988
MaeI	CTAG	3 cut(s)	597, 1011, 1022
MaeII	ACGT	3 cut(s)	1422, 1439, 1491
MaeIII	GTNAC	9 cut(s)	108, 1013, 1036, 1051, 1132, 1153, 1159, 1423, 1498
MalI	GATC	6 cut(s)	198, 769, 836, 936, 947, 1512
MboI	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
MboII	GAAGA	3 cut(s)	287, 452, 1388
MfeI	CAATTG	1 cut(s)	573
MflI	RGATCY	1 cut(s)	1510
MhlI	GDGCHC	1 cut(s)	37
MlsI	TGGCCA	1 cut(s)	1460
MluCI	AATT	9 cut(s)	91, 130, 185, 207, 573, 771, 855, 1147, 1415
MluNI	TGGCCA	1 cut(s)	1460
MlyI	GAGTC	1 cut(s)	1240
Mox20I	TGGCCA	1 cut(s)	1460
Mph1103I	ATGCAT	1 cut(s)	985
MroI	TCCGGA	1 cut(s)	250
MscI	TGGCCA	1 cut(s)	1460
MseI	TTAA	3 cut(s)	663, 1215, 1418
Msp20I	TGGCCA	1 cut(s)	1460
MspCI	CTTAAG	1 cut(s)	662
MspI	CCGG	4 cut(s)	251, 285, 302, 1185
MspR9I	CCNGG	6 cut(s)	8, 303, 744, 975, 1138, 1515
MunI	CAATTG	1 cut(s)	573
Mva1269I	GAATGC	1 cut(s)	902
MvaI	CCWGG	5 cut(s)	8, 744, 975, 1138, 1515
MwoI	GCNNNNNNNGC	9 cut(s)	21, 221, 548, 578, 815, 1007, 1031, 1259, 1268
NciI	CCSGG	1 cut(s)	303
NcoI	CCATGG	1 cut(s)	1461
NdeII	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
NlaIII	CATG	4 cut(s)	596, 1222, 1246, 1465
NlaIV	GGNNCC	5 cut(s)	124, 289, 307, 618, 1512
NmuCI	GTSAC	4 cut(s)	108, 1036, 1051, 1423
NsiI	ATGCAT	1 cut(s)	985
NspI	RCATGY	1 cut(s)	596
PceI	AGGCCT	1 cut(s)	738
PctI	GAATGC	1 cut(s)	902
PfeI	GAWTC	3 cut(s)	322, 328, 506
PflMI	CCANNNNNTGG	1 cut(s)	1456
PfoI	TCCNGGA	1 cut(s)	973
PleI	GAGTC	1 cut(s)	1240
PpsI	GAGTC	1 cut(s)	1240
Ppu21I	YACGTR	1 cut(s)	1440
PpuMI	RGGWCCY	1 cut(s)	1166
Psp124BI	GAGCTC	1 cut(s)	37
Psp5II	RGGWCCY	1 cut(s)	1166
Psp6I	CCWGG	5 cut(s)	6, 742, 973, 1136, 1513
PspFI	CCCAGC	2 cut(s)	930, 1360
PspGI	CCWGG	5 cut(s)	6, 742, 973, 1136, 1513
PspN4I	GGNNCC	5 cut(s)	124, 289, 307, 618, 1512
PspPI	GGNCC	4 cut(s)	4, 1166, 1277, 1382
PspPPI	RGGWCCY	1 cut(s)	1166
PsrI	GAACNNNNNNTAC	2 cut(s)	504, 536
PstI	CTGCAG	1 cut(s)	26
PstNI	CAGNNNCTG	1 cut(s)	683
PsuI	RGATCY	1 cut(s)	1510
RsaI	GTAC	4 cut(s)	606, 785, 965, 1522
RsaNI	GTAC	4 cut(s)	605, 784, 964, 1521
SacI	GAGCTC	1 cut(s)	37
SalI	GTCGAC	1 cut(s)	241
SaqAI	TTAA	3 cut(s)	663, 1215, 1418
Sau3AI	GATC	6 cut(s)	196, 767, 834, 934, 945, 1510
Sau96I	GGNCC	4 cut(s)	4, 1166, 1277, 1382
ScaI	AGTACT	1 cut(s)	785
SchI	GAGTC	1 cut(s)	1240
ScrFI	CCNGG	6 cut(s)	8, 303, 744, 975, 1138, 1515
SduI	GDGCHC	1 cut(s)	37
SfaNI	GCATC	3 cut(s)	51, 670, 988
SfcI	CTRYAG	1 cut(s)	22
SinI	GGWCC	2 cut(s)	1166, 1382
SmlI	CTYRAG	1 cut(s)	662
SmoI	CTYRAG	1 cut(s)	662
Sse9I	AATT	9 cut(s)	91, 130, 185, 207, 573, 771, 855, 1147, 1415
SseBI	AGGCCT	1 cut(s)	738
SsiI	CCGC	2 cut(s)	556, 1406
SspMI	CTAG	3 cut(s)	597, 1011, 1022
SstI	GAGCTC	1 cut(s)	37
StuI	AGGCCT	1 cut(s)	738
StyD4I	CCNGG	6 cut(s)	6, 301, 742, 973, 1136, 1513
StyI	CCWWGG	4 cut(s)	424, 532, 1461, 1555
TaaI	ACNGT	8 cut(s)	178, 247, 364, 923, 1051, 1057, 1159, 1372
TaiI	ACGT	3 cut(s)	1425, 1442, 1494
TaqI	TCGA	7 cut(s)	195, 242, 273, 320, 622, 837, 990
TasI	AATT	9 cut(s)	91, 130, 185, 207, 573, 771, 855, 1147, 1415
TatI	WGTACW	4 cut(s)	604, 783, 963, 1520
TauI	GCSGC	1 cut(s)	559
TfiI	GAWTC	3 cut(s)	322, 328, 506
Tru1I	TTAA	3 cut(s)	663, 1215, 1418
Tru9I	TTAA	3 cut(s)	663, 1215, 1418
TscAI	CASTG	5 cut(s)	918, 928, 1045, 1060, 1351
TseFI	GTSAC	4 cut(s)	108, 1036, 1051, 1423
TseI	GCWGC	9 cut(s)	212, 262, 341, 569, 581, 667, 806, 1031, 1478
Tsp45I	GTSAC	4 cut(s)	108, 1036, 1051, 1423
TspDTI	ATGAA	4 cut(s)	386, 519, 531, 1235
TspGWI	ACGGA	3 cut(s)	707, 855, 1337
TspRI	CASTG	5 cut(s)	918, 928, 1045, 1060, 1351
Van91I	CCANNNNNTGG	1 cut(s)	1456
Vha464I	CTTAAG	1 cut(s)	662
VpaK11BI	GGWCC	2 cut(s)	1166, 1382
XapI	RAATTY	4 cut(s)	91, 130, 185, 1147
XceI	RCATGY	1 cut(s)	596
XcmI	CCANNNNNNNNNTGG	2 cut(s)	868, 1294
XmiI	GTMKAC	1 cut(s)	242
XspI	CTAG	3 cut(s)	597, 1011, 1022
ZrmI	AGTACT	1 cut(s)	785
Zsp2I	ATGCAT	1 cut(s)	985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.