Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004684

Physical Location & Seq

Genomic Coordinates Forward (+)

20946589 .. 20947154

Gene Structure 566 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1207500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 204 bp

ATGAACATGTACAACGACCACAGACAGGCCATGGCATTCTCTCTCGCTGCATGTGGAGATGTTCCGGTTACCATCAAGGATCCCGGTTGTACCAGTAAAACATTCCCTGGTTGCTTTCAAGTCCTTGGGAAGTTTACCAAGCATTGGACAAAGCAATGCAGATGTAATTTTGGATGCTTACATTTTTCAAGTAGAGCACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003866 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

EPSP_synthase

Protein Analysis

68

Amino Acids

7.53

Weight (kDa)

9.1

Isoelectric Point (pI)

38.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EPSP_synthase	PF00275	3 - 42	1.4e-08	EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48860 AT1G48860 AT2G45300 AT2G45300 AT2G45300 AT2G45300
fragaria_vesca	FvH4_7g11530 FvH4_7g11530
malus_domestica	MD02G1207300.v1.1 MD02G1207500.v1.1 MD07G1119600.v1.1 MD10G1038600.v1.1 MD14G1236200.v1.1
prunus_persica	Prupe.2G143700_v2.0.a1
pyrus_communis	pycom02g16870 pycom04g13590 pycom06g15290 pycom07g10510
rosa_chinensis	RchiOBHm_Chr1g0349971
rosa_laevigata	RLG00000028512
rosa_multiflora	Rmu_sc0000778.1_g000001 Rmu_sc0005220.1_g000012 Rmu_sc0039385.1_g000001
rosa_roxburghii	Rroxscaffold_4G00305240
rosa_rugosa	Rorug01G0207400 Rorug01G0207500
rosa_samantha	Rh1AG224000 Rh1BG191200 Rh1CG208700 Rh1DG219800
rosa_wichuraiana	Rw0G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	144
AclWI	GGATC	2 cut(s)	74, 87
AfaI	GTAC	2 cut(s)	11, 91
AfiI	CCNNNNNNNGG	2 cut(s)	25, 144
AflIII	ACRYGT	1 cut(s)	6
AgsI	TTSAA	2 cut(s)	119, 189
AjnI	CCWGG	1 cut(s)	106
Alw21I	GWGCWC	1 cut(s)	199
AlwI	GGATC	2 cut(s)	74, 87
AoxI	GGCC	1 cut(s)	27
ApeKI	GCWGC	1 cut(s)	47
AsuC2I	CCSGG	1 cut(s)	84
BamHI	GGATCC	1 cut(s)	79
Bbv12I	GWGCWC	1 cut(s)	199
BbvI	GCAGC	1 cut(s)	34
BccI	CCATC	1 cut(s)	80
BciT130I	CCWGG	1 cut(s)	108
BcnI	CCSGG	1 cut(s)	84
BisI	GCNGC	1 cut(s)	48
BlsI	GCNGC	1 cut(s)	49
Bme1390I	CCNGG	2 cut(s)	84, 108
BmiI	GGNNCC	1 cut(s)	81
BmrFI	CCNGG	2 cut(s)	84, 108
BmsI	GCATC	1 cut(s)	164
BpuMI	CCSGG	1 cut(s)	84
BsaJI	CCNNGG	3 cut(s)	30, 106, 124
BsaWI	WCCGGW	1 cut(s)	64
Bsc4I	CCNNNNNNNGG	2 cut(s)	25, 144
Bse1I	ACTGG	1 cut(s)	93
Bse3DI	GCAATG	1 cut(s)	161
BseBI	CCWGG	1 cut(s)	108
BseDI	CCNNGG	3 cut(s)	30, 106, 124
BseGI	GGATG	1 cut(s)	179
BseLI	CCNNNNNNNGG	2 cut(s)	25, 144
BseMI	GCAATG	1 cut(s)	161
BseNI	ACTGG	1 cut(s)	93
BseXI	GCAGC	1 cut(s)	34
BshFI	GGCC	1 cut(s)	29
BsiHKAI	GWGCWC	1 cut(s)	199
BsiSI	CCGG	2 cut(s)	65, 84
BslI	CCNNNNNNNGG	2 cut(s)	25, 144
BsmI	GAATGC	1 cut(s)	35
BsnI	GGCC	1 cut(s)	29
Bsp1286I	GDGCHC	1 cut(s)	199
Bsp1407I	TGTACA	1 cut(s)	9
Bsp143I	GATC	1 cut(s)	79
Bsp19I	CCATGG	1 cut(s)	30
BspANI	GGCC	1 cut(s)	29
BspLI	GGNNCC	1 cut(s)	81
BspPI	GGATC	2 cut(s)	74, 87
BsrDI	GCAATG	1 cut(s)	161
BsrGI	TGTACA	1 cut(s)	9
BsrI	ACTGG	1 cut(s)	93
BssECI	CCNNGG	3 cut(s)	30, 106, 124
BssMI	GATC	1 cut(s)	79
BssT1I	CCWWGG	2 cut(s)	30, 124
Bst2UI	CCWGG	1 cut(s)	108
BstAUI	TGTACA	1 cut(s)	9
BstDSI	CCRYGG	1 cut(s)	30
BstEII	GGTNACC	1 cut(s)	67
BstF5I	GGATG	1 cut(s)	179
BstKTI	GATC	1 cut(s)	82
BstMBI	GATC	1 cut(s)	79
BstNI	CCWGG	1 cut(s)	108
BstNSI	RCATGY	2 cut(s)	10, 54
BstPI	GGTNACC	1 cut(s)	67
BstSCI	CCNGG	2 cut(s)	82, 106
BstV1I	GCAGC	1 cut(s)	34
BstX2I	RGATCY	1 cut(s)	79
BstYI	RGATCY	1 cut(s)	79
BsuRI	GGCC	1 cut(s)	29
BtgI	CCRYGG	1 cut(s)	30
BtsCI	GGATG	1 cut(s)	179
Csp6I	GTAC	2 cut(s)	10, 90
CviAII	CATG	3 cut(s)	7, 31, 51
CviJI	RGCY	1 cut(s)	29
CviKI_1	RGCY	1 cut(s)	29
CviQI	GTAC	2 cut(s)	10, 90
DpnI	GATC	1 cut(s)	81
DpnII	GATC	1 cut(s)	79
Eco130I	CCWWGG	2 cut(s)	30, 124
Eco91I	GGTNACC	1 cut(s)	67
EcoO65I	GGTNACC	1 cut(s)	67
EcoRII	CCWGG	1 cut(s)	106
EcoT14I	CCWWGG	2 cut(s)	30, 124
ErhI	CCWWGG	2 cut(s)	30, 124
FaeI	CATG	3 cut(s)	10, 34, 54
FaiI	YATR	3 cut(s)	8, 32, 52
FatI	CATG	3 cut(s)	6, 30, 50
Fnu4HI	GCNGC	1 cut(s)	48
FokI	GGATG	1 cut(s)	186
Fsp4HI	GCNGC	1 cut(s)	48
GluI	GCNGC	1 cut(s)	48
HaeIII	GGCC	1 cut(s)	29
HapII	CCGG	2 cut(s)	65, 84
Hin1II	CATG	3 cut(s)	10, 34, 54
HpaII	CCGG	2 cut(s)	65, 84
Hpy166II	GTNNAC	1 cut(s)	135
Hpy8I	GTNNAC	1 cut(s)	135
HpyCH4V	TGCA	2 cut(s)	50, 159
Hsp92II	CATG	3 cut(s)	10, 34, 54
Kzo9I	GATC	1 cut(s)	79
LpnPI	CCDG	6 cut(s)	11, 78, 93, 97, 106, 120
Lsp1109I	GCAGC	1 cut(s)	34
LweI	GCATC	1 cut(s)	164
MaeIII	GTNAC	1 cut(s)	67
MalI	GATC	1 cut(s)	81
MboI	GATC	1 cut(s)	79
MflI	RGATCY	1 cut(s)	79
MhlI	GDGCHC	1 cut(s)	199
MluCI	AATT	1 cut(s)	166
MseI	TTAA	1 cut(s)	202
MspI	CCGG	2 cut(s)	65, 84
MspR9I	CCNGG	2 cut(s)	84, 108
Mva1269I	GAATGC	1 cut(s)	35
MvaI	CCWGG	1 cut(s)	108
NciI	CCSGG	1 cut(s)	84
NcoI	CCATGG	1 cut(s)	30
NdeII	GATC	1 cut(s)	79
NlaIII	CATG	3 cut(s)	10, 34, 54
NlaIV	GGNNCC	1 cut(s)	81
NspI	RCATGY	2 cut(s)	10, 54
PciI	ACATGT	1 cut(s)	6
PctI	GAATGC	1 cut(s)	35
PflMI	CCANNNNNTGG	1 cut(s)	144
PkrI	GCNGC	1 cut(s)	49
PscI	ACATGT	1 cut(s)	6
Psp6I	CCWGG	1 cut(s)	106
PspEI	GGTNACC	1 cut(s)	67
PspGI	CCWGG	1 cut(s)	106
PspN4I	GGNNCC	1 cut(s)	81
PsuI	RGATCY	1 cut(s)	79
RsaI	GTAC	2 cut(s)	11, 91
RsaNI	GTAC	2 cut(s)	10, 90
SaqAI	TTAA	1 cut(s)	202
SatI	GCNGC	1 cut(s)	48
Sau3AI	GATC	1 cut(s)	79
ScrFI	CCNGG	2 cut(s)	84, 108
SduI	GDGCHC	1 cut(s)	199
SfaNI	GCATC	1 cut(s)	164
Sse9I	AATT	1 cut(s)	166
StyD4I	CCNGG	2 cut(s)	82, 106
StyI	CCWWGG	2 cut(s)	30, 124
TasI	AATT	1 cut(s)	166
TatI	WGTACW	1 cut(s)	9
Tru1I	TTAA	1 cut(s)	202
Tru9I	TTAA	1 cut(s)	202
TseI	GCWGC	1 cut(s)	47
TspDTI	ATGAA	1 cut(s)	17
Van91I	CCANNNNNTGG	1 cut(s)	144
XceI	RCATGY	2 cut(s)	10, 54

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.