Rw0G007590

3-phosphoshikimate 1-carboxyvinyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00272
OG0004684
Physical Location & Seq
Reverse (-)
12797 .. 17369
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007590.1

Sequence Viewer

Length: 1278 bp
ATGGCCCAAGTGAGCAAAATCTGTGGCAATGGAGCTCAAAGCATCAATCTTTTGCCCAATGTCTCCAAACCCCAGATACCCAAATCGTTAAATTCAATCTCTTTGAAGTCACAGTTTTTGGGTTCCTCAAATTCTGTGAGTTTGAAGATGAAAAATGGGTTTGTGGGTGGTAAAGCCAGGGCTGGTGGGCTTAGAGTTTCGGCTTCAGTTGTTACAGCAGAGAAGCCGTCGACGGTGCCGGAGATTGTGTTGCAACCCATCAAAGAAATTTCGGGTACCATAACTTTGCCGGGTTCTAAGTCCTTGTCCAATCGGATTCTGCTTATTGCTGCTCTTTCTGAGGGAACAACTGTTGTAGACAACTTACTGGATAGTGATGATATCAATTATATGCTTGGTGCGTTGAAAACGCTTGGGCTAAATGTGGAAGATGACAAGGCAAACAAAAGAGCTGTTGTGGAGGGTTGTGGTGGTCAGTTTCCGATGGGGAATAAATCCGGAGAGGAAGTTCAACTTTTCCTTGGAAATGCTGGAACAGCAATGAGGCCTTTGACTGCGGCAGTAACTGCTGCTGGTGGACATTCTAGGTATGTACTTGATGGGGTGCCCCGAATGAGGGAGAGACCAATTGGAGATTTGGTCACTGGTCTTAAGCAGCTTGGTGCAGATGTTGACTGTTTTCTTGGAACAGATTGTCCTCCTGTTCGTGTAATTGGAAAGGGAGGCCTTCCAGGAGGCAAGGTGAAACTCTCTGGATCAATCAGTAGTCAATATTTGACTTCTTTGCTTATGGCGGCTCCTTTGGCTCTGGGAGATGTTGAAGTTGAGATTATTGATAAGCTGATTTCCATTCCATATGTTGAAATGACTTTGAAATTGATGGAACGCTTTGGGGTCACAGTGGAACATAGTGATAGCTGGGATCGATTTTTGGTCCGAGGAGGTCAAAAATACAAGTCTCCTGGAAAAGCCTTTGTCGAAGGTGATGCTTCAAGTGCTAGTTACTTTCTAGCTGGTGCTGCAGTAACGGGTGGGACTGTAACTGTTGAAGGCTGTGGCACAAGCAGTTTACAGGGAGATGTAAAATTTGCTGAAGTTCTTGAAAAGATGGGCGCTGAAGTTACCTGGACAGAGAATAGTGTCACCGTCACAGGACCTCCACGACATTCTTCTGGAAGAAAGCACTTGAAAGCTGTTGATGTCAACATGAACAAGATGCCAGATGTTGCCATGACTCTTGCTGTAGTTGCTATTTTGCCGATGGTCCAACTGCCATAA

Protein Analysis

425

Amino Acids

44.45

Weight (kDa)

8.65

Isoelectric Point (pI)

26.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 85 - 418 4.9e-100 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 3 cut(s) 235, 275, 604
AccI GTMKAC 2 cut(s) 230, 357
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 2 cut(s) 557, 794
AclWI GGATC 2 cut(s) 763, 930
AcsI RAATTY 4 cut(s) 91, 130, 267, 1085
AcuI CTGAAG 3 cut(s) 189, 1113, 1137
AfaI GTAC 2 cut(s) 277, 594
AfiI CCNNNNNNNGG 2 cut(s) 615, 616
AflII CTTAAG 1 cut(s) 650
AjnI CCWGG 4 cut(s) 176, 730, 961, 1124
AloI GAACNNNNNNTCC 4 cut(s) 492, 524, 679, 711
AluBI AGCT 7 cut(s) 35, 452, 658, 841, 918, 1013, 1193
AluI AGCT 7 cut(s) 35, 452, 658, 841, 918, 1013, 1193
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 3 cut(s) 67, 616, 963
AlwI GGATC 2 cut(s) 763, 930
AlwNI CAGNNNCTG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 3 cut(s) 3, 545, 724
ApeKI GCWGC 4 cut(s) 329, 569, 655, 1019
ApoI RAATTY 4 cut(s) 91, 130, 267, 1085
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 1115
AspS9I GGNCC 4 cut(s) 4, 934, 1154, 1264
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 3 cut(s) 754, 995, 1135
AvaII GGWCC 3 cut(s) 934, 1154, 1264
BaeGI GKGCMC 1 cut(s) 609
BanI GGYRCC 3 cut(s) 235, 275, 604
BanII GRGCYC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 4 cut(s) 316, 556, 667, 1006
BccI CCATC 6 cut(s) 266, 478, 593, 874, 1102, 1255
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 968, 1002
BciT130I CCWGG 4 cut(s) 178, 732, 963, 1126
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 3 cut(s) 67, 616, 963
BfaI CTAG 3 cut(s) 585, 999, 1010
BfmI CTRYAG 2 cut(s) 1020, 1242
BfoI RGCGCY 1 cut(s) 1116
BfrI CTTAAG 1 cut(s) 650
BisI GCNGC 6 cut(s) 330, 558, 570, 656, 795, 1020
BlsI GCNGC 6 cut(s) 331, 559, 571, 657, 796, 1021
Bme1390I CCNGG 5 cut(s) 178, 291, 732, 963, 1126
Bme18I GGWCC 3 cut(s) 934, 1154, 1264
BmgT120I GGNCC 4 cut(s) 4, 934, 1154, 1264
BmiI GGNNCC 5 cut(s) 124, 237, 277, 606, 798
BmrFI CCNGG 5 cut(s) 178, 291, 732, 963, 1126
BmsI GCATC 3 cut(s) 51, 976, 1206
BpuMI CCSGG 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 925
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 3 cut(s) 177, 520, 937
BsaWI WCCGGW 1 cut(s) 497
Bsc4I CCNNNNNNNGG 2 cut(s) 615, 616
Bse1I ACTGG 2 cut(s) 372, 649
Bse3DI GCAATG 2 cut(s) 34, 546
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 4 cut(s) 178, 732, 963, 1126
BseCI ATCGAT 1 cut(s) 925
BseDI CCNNGG 3 cut(s) 177, 520, 937
BseLI CCNNNNNNNGG 2 cut(s) 615, 616
BseMI GCAATG 2 cut(s) 34, 546
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 2 cut(s) 372, 649
BseRI GAGGAG 1 cut(s) 954
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 4 cut(s) 316, 556, 667, 1006
BseYI CCCAGC 1 cut(s) 918
BsgI GTGCAG 1 cut(s) 684
BshFI GGCC 3 cut(s) 5, 547, 726
BshNI GGYRCC 3 cut(s) 235, 275, 604
BshVI ATCGAT 1 cut(s) 925
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 3 cut(s) 239, 290, 498
BslFI GGGAC 1 cut(s) 1048
BslI CCNNNNNNNGG 2 cut(s) 615, 616
BsmAI GTCTC 3 cut(s) 67, 616, 963
BsmFI GGGAC 1 cut(s) 1048
BsnI GGCC 3 cut(s) 5, 547, 726
Bso31I GGTCTC 1 cut(s) 616
Bsp1286I GDGCHC 2 cut(s) 37, 609
Bsp13I TCCGGA 1 cut(s) 497
Bsp143I GATC 2 cut(s) 755, 922
BspACI CCGC 2 cut(s) 557, 794
BspANI GGCC 3 cut(s) 5, 547, 726
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 925
BspEI TCCGGA 1 cut(s) 497
BspLI GGNNCC 5 cut(s) 124, 237, 277, 606, 798
BspMAI CTGCAG 1 cut(s) 1024
BspPI GGATC 2 cut(s) 763, 930
BspT107I GGYRCC 3 cut(s) 235, 275, 604
BspTI CTTAAG 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 616
BsrDI GCAATG 2 cut(s) 34, 546
BsrI ACTGG 2 cut(s) 372, 649
BssECI CCNNGG 3 cut(s) 177, 520, 937
BssMI GATC 2 cut(s) 755, 922
BssT1I CCWWGG 1 cut(s) 520
Bst2UI CCWGG 4 cut(s) 178, 732, 963, 1126
Bst4CI ACNGT 8 cut(s) 114, 235, 352, 677, 901, 1039, 1045, 1147
BstAFI CTTAAG 1 cut(s) 650
BstAPI GCANNNNNTGC 1 cut(s) 566
BstDEI CTNAG 3 cut(s) 191, 297, 339
BstH2I RGCGCY 1 cut(s) 1116
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 2 cut(s) 758, 925
BstMAI GTCTC 3 cut(s) 67, 616, 963
BstMBI GATC 2 cut(s) 755, 922
BstMWI GCNNNNNNNGC 6 cut(s) 536, 566, 803, 995, 1019, 1247
BstNI CCWGG 4 cut(s) 178, 732, 963, 1126
BstSCI CCNGG 5 cut(s) 176, 289, 730, 961, 1124
BstSFI CTRYAG 2 cut(s) 1020, 1242
BstSLI GKGCMC 1 cut(s) 609
BstV1I GCAGC 4 cut(s) 316, 556, 667, 1006
Bsu15I ATCGAT 1 cut(s) 925
BsuRI GGCC 3 cut(s) 5, 547, 726
BsuTUI ATCGAT 1 cut(s) 925
BtsIMutI CAGTG 2 cut(s) 642, 906
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 1115
Cfr13I GGNCC 4 cut(s) 4, 934, 1154, 1264
ClaI ATCGAT 1 cut(s) 925
Csp6I GTAC 2 cut(s) 276, 593
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 2 cut(s) 1207, 1231
CviQI GTAC 2 cut(s) 276, 593
DdeI CTNAG 3 cut(s) 191, 297, 339
DpnI GATC 2 cut(s) 757, 924
DpnII GATC 2 cut(s) 755, 922
Ecl136II GAGCTC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 520
Eco147I AGGCCT 2 cut(s) 547, 726
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 616
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 3 cut(s) 934, 1154, 1264
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 3 cut(s) 189, 1113, 1137
EcoICRI GAGCTC 1 cut(s) 35
EcoO109I RGGNCCY 1 cut(s) 1154
EcoRII CCWGG 4 cut(s) 176, 730, 961, 1124
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 2 cut(s) 1210, 1234
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 1 cut(s) 1048
FatI CATG 2 cut(s) 1206, 1230
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 2 cut(s) 230, 357
Fnu4HI GCNGC 6 cut(s) 330, 558, 570, 656, 795, 1020
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 6 cut(s) 330, 558, 570, 656, 795, 1020
FspBI CTAG 3 cut(s) 585, 999, 1010
GlaI GCGC 1 cut(s) 1114
GluI GCNGC 6 cut(s) 330, 558, 570, 656, 795, 1020
GsaI CCCAGC 1 cut(s) 922
HaeII RGCGCY 1 cut(s) 1116
HaeIII GGCC 3 cut(s) 5, 547, 726
HapII CCGG 3 cut(s) 239, 290, 498
HhaI GCGC 1 cut(s) 1115
Hin1II CATG 2 cut(s) 1210, 1234
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HincII GTYRAC 3 cut(s) 231, 673, 1204
HindII GTYRAC 3 cut(s) 231, 673, 1204
HinfI GANTC 2 cut(s) 316, 1234
HpaII CCGG 3 cut(s) 239, 290, 498
HphI GGTGA 3 cut(s) 754, 995, 1135
Hpy166II GTNNAC 6 cut(s) 231, 358, 578, 673, 1070, 1204
Hpy188I TCNGA 4 cut(s) 315, 340, 483, 938
Hpy188III TCNNGA 4 cut(s) 498, 753, 1100, 1173
Hpy8I GTNNAC 6 cut(s) 231, 358, 578, 673, 1070, 1204
Hpy99I CGWCG 2 cut(s) 232, 235
HpyAV CCTTC 3 cut(s) 737, 974, 1043
HpyCH4III ACNGT 8 cut(s) 114, 235, 352, 677, 901, 1039, 1045, 1147
HpyCH4V TGCA 3 cut(s) 253, 665, 1022
HpyF10VI GCNNNNNNNGC 6 cut(s) 536, 566, 803, 995, 1019, 1247
HpyF3I CTNAG 3 cut(s) 191, 297, 339
Hsp92II CATG 2 cut(s) 1210, 1234
HspAI GCGC 1 cut(s) 1113
Kpn2I TCCGGA 1 cut(s) 497
KpnI GGTACC 1 cut(s) 279
Kzo9I GATC 2 cut(s) 755, 922
LmnI GCTCC 2 cut(s) 32, 802
Lsp1109I GCAGC 4 cut(s) 316, 556, 667, 1006
LweI GCATC 3 cut(s) 51, 976, 1206
MaeI CTAG 3 cut(s) 585, 999, 1010
MalI GATC 2 cut(s) 757, 924
MboI GATC 2 cut(s) 755, 922
MboII GAAGA 4 cut(s) 157, 440, 1161, 1188
MfeI CAATTG 1 cut(s) 627
MhlI GDGCHC 2 cut(s) 37, 609
MluCI AATT 8 cut(s) 91, 130, 267, 385, 627, 711, 875, 1085
MlyI GAGTC 1 cut(s) 1228
MroI TCCGGA 1 cut(s) 497
MseI TTAA 2 cut(s) 89, 651
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 3 cut(s) 239, 290, 498
MspR9I CCNGG 5 cut(s) 178, 291, 732, 963, 1126
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 4 cut(s) 178, 732, 963, 1126
MwoI GCNNNNNNNGC 6 cut(s) 536, 566, 803, 995, 1019, 1247
NciI CCSGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 856
NdeII GATC 2 cut(s) 755, 922
NlaIII CATG 2 cut(s) 1210, 1234
NlaIV GGNNCC 5 cut(s) 124, 237, 277, 606, 798
NmuCI GTSAC 5 cut(s) 108, 640, 895, 1141, 1147
PceI AGGCCT 2 cut(s) 547, 726
PfeI GAWTC 1 cut(s) 316
PfoI TCCNGGA 2 cut(s) 730, 961
PkrI GCNGC 6 cut(s) 331, 559, 571, 657, 796, 1021
PleI GAGTC 1 cut(s) 1228
PpsI GAGTC 1 cut(s) 1228
PpuMI RGGWCCY 1 cut(s) 1154
Psp124BI GAGCTC 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 1154
Psp6I CCWGG 4 cut(s) 176, 730, 961, 1124
PspFI CCCAGC 1 cut(s) 918
PspGI CCWGG 4 cut(s) 176, 730, 961, 1124
PspN4I GGNNCC 5 cut(s) 124, 237, 277, 606, 798
PspPI GGNCC 4 cut(s) 4, 934, 1154, 1264
PspPPI RGGWCCY 1 cut(s) 1154
PstI CTGCAG 1 cut(s) 1024
PstNI CAGNNNCTG 1 cut(s) 566
RsaI GTAC 2 cut(s) 277, 594
RsaNI GTAC 2 cut(s) 276, 593
SacI GAGCTC 1 cut(s) 37
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 2 cut(s) 89, 651
SatI GCNGC 6 cut(s) 330, 558, 570, 656, 795, 1020
Sau3AI GATC 2 cut(s) 755, 922
Sau96I GGNCC 4 cut(s) 4, 934, 1154, 1264
SchI GAGTC 1 cut(s) 1228
ScrFI CCNGG 5 cut(s) 178, 291, 732, 963, 1126
SduI GDGCHC 2 cut(s) 37, 609
SfaNI GCATC 3 cut(s) 51, 976, 1206
SfcI CTRYAG 2 cut(s) 1020, 1242
SgrDI CGTCGACG 1 cut(s) 229
SinI GGWCC 3 cut(s) 934, 1154, 1264
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 8 cut(s) 91, 130, 267, 385, 627, 711, 875, 1085
SseBI AGGCCT 2 cut(s) 547, 726
SsiI CCGC 2 cut(s) 557, 794
SspI AATATT 1 cut(s) 773
SspMI CTAG 3 cut(s) 585, 999, 1010
SstI GAGCTC 1 cut(s) 37
StuI AGGCCT 2 cut(s) 547, 726
StyD4I CCNGG 5 cut(s) 176, 289, 730, 961, 1124
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 8 cut(s) 114, 235, 352, 677, 901, 1039, 1045, 1147
TaqI TCGA 3 cut(s) 230, 925, 978
TasI AATT 8 cut(s) 91, 130, 267, 385, 627, 711, 875, 1085
TatI WGTACW 1 cut(s) 592
TauI GCSGC 2 cut(s) 560, 797
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 2 cut(s) 89, 651
Tru9I TTAA 2 cut(s) 89, 651
TscAI CASTG 2 cut(s) 649, 906
TseFI GTSAC 5 cut(s) 108, 640, 895, 1141, 1147
TseI GCWGC 4 cut(s) 329, 569, 655, 1019
Tsp45I GTSAC 5 cut(s) 108, 640, 895, 1141, 1147
TspDTI ATGAA 2 cut(s) 164, 1223
TspRI CASTG 2 cut(s) 649, 906
Vha464I CTTAAG 1 cut(s) 650
VpaK11BI GGWCC 3 cut(s) 934, 1154, 1264
XapI RAATTY 4 cut(s) 91, 130, 267, 1085
XmiI GTMKAC 2 cut(s) 230, 357
XspI CTAG 3 cut(s) 585, 999, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.