Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004684

Physical Location & Seq

Genomic Coordinates Reverse (-)

5022941 .. 5023274

Gene Structure 334 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1038600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 249 bp

ATGATTGCCATTTGCACTGAACTAAGAAAGCTAGGAGCAACTGTTGAAGAAGGACCAGATTACTGCATAATCACACCGCCAGAAAAATTAAACGTGACAGCAATAGACACGTATGATGACCACCGAATGGCCATGGCTTTCTCTCTTGCTTCCTGTGGAGACGTTCCAGTTACTATCAATGATCCTGGTTGTACACGAAAAACATTCCCCGATTACTTTGAAGTCCTTGGGAGGTTCACCAAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003866 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

EPSP_synthase

Protein Analysis

83

Amino Acids

9.11

Weight (kDa)

4.91

Isoelectric Point (pI)

40.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EPSP_synthase	PF00275	2 - 76	2.6e-24	EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48860 AT1G48860 AT2G45300 AT2G45300 AT2G45300 AT2G45300
fragaria_vesca	FvH4_7g11530 FvH4_7g11530
malus_domestica	MD02G1207300.v1.1 MD02G1207500.v1.1 MD07G1119600.v1.1 MD10G1038600.v1.1 MD14G1236200.v1.1
prunus_persica	Prupe.2G143700_v2.0.a1
pyrus_communis	pycom02g16870 pycom04g13590 pycom06g15290 pycom07g10510
rosa_chinensis	RchiOBHm_Chr1g0349971
rosa_laevigata	RLG00000028512
rosa_multiflora	Rmu_sc0000778.1_g000001 Rmu_sc0005220.1_g000012 Rmu_sc0039385.1_g000001
rosa_roxburghii	Rroxscaffold_4G00305240
rosa_rugosa	Rorug01G0207400 Rorug01G0207500
rosa_samantha	Rh1AG224000 Rh1BG191200 Rh1CG208700 Rh1DG219800
rosa_wichuraiana	Rw0G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	127
AciI	CCGC	1 cut(s)	77
AclWI	GGATC	1 cut(s)	176
AcoI	YGGCCR	1 cut(s)	129
AfaI	GTAC	1 cut(s)	193
AfiI	CCNNNNNNNGG	1 cut(s)	127
AflIII	ACRYGT	1 cut(s)	108
AgsI	TTSAA	2 cut(s)	47, 221
AjnI	CCWGG	1 cut(s)	184
AluBI	AGCT	1 cut(s)	31
AluI	AGCT	1 cut(s)	31
Alw26I	GTCTC	1 cut(s)	153
AlwI	GGATC	1 cut(s)	176
AoxI	GGCC	1 cut(s)	129
AspS9I	GGNCC	1 cut(s)	53
AsuHPI	GGTGA	1 cut(s)	229
AvaII	GGWCC	1 cut(s)	53
BalI	TGGCCA	1 cut(s)	131
BciT130I	CCWGG	1 cut(s)	186
BcoDI	GTCTC	1 cut(s)	153
BfaI	CTAG	1 cut(s)	32
Bme1390I	CCNGG	1 cut(s)	186
Bme18I	GGWCC	1 cut(s)	53
BmgT120I	GGNCC	1 cut(s)	53
BmrFI	CCNGG	1 cut(s)	186
BsaAI	YACGTR	1 cut(s)	111
BsaJI	CCNNGG	2 cut(s)	132, 226
BsaXI	ACNNNNNCTCC	2 cut(s)	27, 57
Bsc4I	CCNNNNNNNGG	1 cut(s)	127
Bse1I	ACTGG	1 cut(s)	167
BseBI	CCWGG	1 cut(s)	186
BseDI	CCNNGG	2 cut(s)	132, 226
BseLI	CCNNNNNNNGG	1 cut(s)	127
BseNI	ACTGG	1 cut(s)	167
BshFI	GGCC	1 cut(s)	131
BslI	CCNNNNNNNGG	1 cut(s)	127
BsmAI	GTCTC	1 cut(s)	153
BsmBI	CGTCTC	1 cut(s)	153
BsnI	GGCC	1 cut(s)	131
Bsp1407I	TGTACA	1 cut(s)	191
Bsp143I	GATC	1 cut(s)	181
Bsp19I	CCATGG	1 cut(s)	132
BspACI	CCGC	1 cut(s)	77
BspANI	GGCC	1 cut(s)	131
BspPI	GGATC	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	191
BsrI	ACTGG	1 cut(s)	167
BssECI	CCNNGG	2 cut(s)	132, 226
BssMI	GATC	1 cut(s)	181
BssT1I	CCWWGG	2 cut(s)	132, 226
Bst2UI	CCWGG	1 cut(s)	186
Bst4CI	ACNGT	1 cut(s)	43
BstAUI	TGTACA	1 cut(s)	191
BstBAI	YACGTR	1 cut(s)	111
BstDEI	CTNAG	1 cut(s)	23
BstDSI	CCRYGG	1 cut(s)	132
BstKTI	GATC	1 cut(s)	184
BstMAI	GTCTC	1 cut(s)	153
BstMBI	GATC	1 cut(s)	181
BstNI	CCWGG	1 cut(s)	186
BstSCI	CCNGG	1 cut(s)	184
BsuRI	GGCC	1 cut(s)	131
BtgI	CCRYGG	1 cut(s)	132
BtsIMutI	CAGTG	1 cut(s)	15
Cfr13I	GGNCC	1 cut(s)	53
Csp6I	GTAC	1 cut(s)	192
CviAII	CATG	1 cut(s)	133
CviJI	RGCY	3 cut(s)	31, 131, 137
CviKI_1	RGCY	3 cut(s)	31, 131, 137
CviQI	GTAC	1 cut(s)	192
DdeI	CTNAG	1 cut(s)	23
DpnI	GATC	1 cut(s)	183
DpnII	GATC	1 cut(s)	181
EaeI	YGGCCR	1 cut(s)	129
Eco130I	CCWWGG	2 cut(s)	132, 226
Eco47I	GGWCC	1 cut(s)	53
EcoRII	CCWGG	1 cut(s)	184
EcoT14I	CCWWGG	2 cut(s)	132, 226
ErhI	CCWWGG	2 cut(s)	132, 226
Esp3I	CGTCTC	1 cut(s)	153
FaeI	CATG	1 cut(s)	136
FaiI	YATR	3 cut(s)	68, 114, 134
FatI	CATG	1 cut(s)	132
FspBI	CTAG	1 cut(s)	32
HaeIII	GGCC	1 cut(s)	131
Hin1II	CATG	1 cut(s)	136
HphI	GGTGA	1 cut(s)	229
Hpy166II	GTNNAC	2 cut(s)	194, 237
Hpy8I	GTNNAC	2 cut(s)	194, 237
HpyAV	CCTTC	1 cut(s)	44
HpyCH4III	ACNGT	1 cut(s)	43
HpyCH4IV	ACGT	3 cut(s)	93, 110, 162
HpyCH4V	TGCA	2 cut(s)	15, 66
HpyF3I	CTNAG	1 cut(s)	23
HpySE526I	ACGT	3 cut(s)	93, 110, 162
Hsp92II	CATG	1 cut(s)	136
Kzo9I	GATC	1 cut(s)	181
LmnI	GCTCC	1 cut(s)	35
LpnPI	CCDG	6 cut(s)	69, 93, 166, 171, 180, 198
MaeI	CTAG	1 cut(s)	32
MaeII	ACGT	3 cut(s)	93, 110, 162
MaeIII	GTNAC	2 cut(s)	94, 169
MalI	GATC	1 cut(s)	183
MboI	GATC	1 cut(s)	181
MboII	GAAGA	1 cut(s)	59
MlsI	TGGCCA	1 cut(s)	131
MluCI	AATT	1 cut(s)	86
MluNI	TGGCCA	1 cut(s)	131
MnlI	CCTC	1 cut(s)	225
Mox20I	TGGCCA	1 cut(s)	131
MscI	TGGCCA	1 cut(s)	131
MseI	TTAA	1 cut(s)	89
Msp20I	TGGCCA	1 cut(s)	131
MspR9I	CCNGG	1 cut(s)	186
MvaI	CCWGG	1 cut(s)	186
NcoI	CCATGG	1 cut(s)	132
NdeII	GATC	1 cut(s)	181
NlaIII	CATG	1 cut(s)	136
NmuCI	GTSAC	1 cut(s)	94
PflMI	CCANNNNNTGG	1 cut(s)	127
Ppu21I	YACGTR	1 cut(s)	111
Psp6I	CCWGG	1 cut(s)	184
PspGI	CCWGG	1 cut(s)	184
PspPI	GGNCC	1 cut(s)	53
RsaI	GTAC	1 cut(s)	193
RsaNI	GTAC	1 cut(s)	192
SaqAI	TTAA	1 cut(s)	89
Sau3AI	GATC	1 cut(s)	181
Sau96I	GGNCC	1 cut(s)	53
ScrFI	CCNGG	1 cut(s)	186
SetI	ASST	5 cut(s)	33, 96, 113, 165, 236
SinI	GGWCC	1 cut(s)	53
Sse9I	AATT	1 cut(s)	86
SsiI	CCGC	1 cut(s)	77
SspMI	CTAG	1 cut(s)	32
StyD4I	CCNGG	1 cut(s)	184
StyI	CCWWGG	2 cut(s)	132, 226
TaaI	ACNGT	1 cut(s)	43
TaiI	ACGT	3 cut(s)	96, 113, 165
TasI	AATT	1 cut(s)	86
TatI	WGTACW	1 cut(s)	191
Tru1I	TTAA	1 cut(s)	89
Tru9I	TTAA	1 cut(s)	89
TscAI	CASTG	1 cut(s)	22
TseFI	GTSAC	1 cut(s)	94
Tsp45I	GTSAC	1 cut(s)	94
TspRI	CASTG	1 cut(s)	22
Van91I	CCANNNNNTGG	1 cut(s)	127
VpaK11BI	GGWCC	1 cut(s)	53
XspI	CTAG	1 cut(s)	32

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.