MD02G1210500.v1.1

Vesicle-associated membrane protein-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010691
Physical Location & Seq
Reverse (-)
21934719 .. 21938787
4069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1210500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGAGCACCGGCGAGCTTCTCAGAATCGAACCCCAAGAGCTCCAGTTCCCCTTTGAATTGAGGAAGCAGATCTCTTGCTCTCTGCAACTCTCCAACAAAACCAATGACTATGTGGCTTTCAAGGTAAAAACAACAAATCCCAAAAAGTATTGTGTTAGACCAAACACTGGGGTTGTGTTGCCAAGGTCCACTTCTGATGTTATAGTGACAATGCAAGCGCAAAAGGAGGCACCTTCGGATATGCAATGCAAGGACAAGTTCCTTCTTCAGAGTGTCGTTGCCAGCCCCGGGACTACCACAAAGGACATCACCGCAGAGATGTTCAATAAGGAATCGGGGCATAATGTTGAAGAGTGTAAATTGAGGGTTCTCTATGTTGCGCCGCCTCGGCCACCATCGCCGGTTCGCGAAGGTTCAGAGGAAGGTTCGTCACCCAGGGCTTCAGTGGACAACGGGAGTGCAGCAGCTTCTGAGTTCAAAGCTGCTTCAAGAGCTTTCTCTGAGCAACATGAGCATCAAGATAACTCGTCTGAGGCAAGGACATTAATTGCGAGGCTGACTGAGGAGAAAATTTCTGCCATTCAGCAAAATAACAGGCTTCAACAAGAACTGGATGTTTTGAGGCGCGGGGCCAACAAGAGTGCTGGTGGCATTCCATTTATCTATGTTCTTCTAGTTGGCTTGATTGGCATTATTTTGGGGTACATTCTGAAGAAGACATGA

Protein Analysis

241

Amino Acids

26.48

Weight (kDa)

8.83

Isoelectric Point (pI)

58.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 7 - 111 8.3e-33 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010691)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45140 AT2G45140 AT3G60600 AT3G60600
fragaria_vesca FvH4_7g11090
malus_domestica MD02G1210500.v1.1 MD07G1115600.v1.1
prunus_persica Prupe.2G138100_v2.0.a1
pyrus_communis pycom02g17230 pycom07g10890
rosa_chinensis RchiOBHm_Chr1g0349111
rosa_laevigata RLG00000028605
rosa_multiflora Rmu_sc0008324.1_g000004 Rmu_sc0012100.1_g000006
rosa_roxburghii Rroxscaffold_4G00306110
rosa_rugosa Rorug01G0200200
rosa_samantha Rh1AG217900 Rh1BG183500 Rh1CG202300
rosa_wichuraiana Rw1G018520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 1 cut(s) 167
AccII CGCG 2 cut(s) 408, 627
AciI CCGC 3 cut(s) 312, 383, 627
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 2 cut(s) 251, 426
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 2 cut(s) 167, 288
AgsI TTSAA 7 cut(s) 56, 121, 325, 350, 478, 489, 602
AjnI CCWGG 1 cut(s) 434
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 5 cut(s) 16, 40, 467, 482, 494
AluI AGCT 5 cut(s) 16, 40, 467, 482, 494
Alw21I GWGCWC 2 cut(s) 8, 42
AlwNI CAGNNNCTG 1 cut(s) 470
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 389, 630
ApeKI GCWGC 3 cut(s) 461, 464, 482
ApoI RAATTY 1 cut(s) 570
AseI ATTAAT 1 cut(s) 545
AspLEI GCGC 3 cut(s) 220, 382, 627
AspS9I GGNCC 2 cut(s) 186, 630
AsuC2I CCSGG 2 cut(s) 288, 289
AsuHPI GGTGA 2 cut(s) 301, 423
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 8, 42
BbvI GCAGC 3 cut(s) 469, 473, 476
BccI CCATC 1 cut(s) 403
BciT130I CCWGG 1 cut(s) 436
BcnI CCSGG 2 cut(s) 288, 289
BfaI CTAG 1 cut(s) 674
BglI GCCNNNNNGGC 1 cut(s) 388
BglII AGATCT 1 cut(s) 69
BisI GCNGC 4 cut(s) 383, 462, 465, 483
BlsI GCNGC 4 cut(s) 384, 463, 466, 484
Bme1390I CCNGG 3 cut(s) 288, 289, 436
Bme18I GGWCC 1 cut(s) 186
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 186, 630
BmiI GGNNCC 2 cut(s) 231, 631
BmrFI CCNGG 3 cut(s) 288, 289, 436
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 523
BmuI ACTGGG 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 26
BpuMI CCSGG 2 cut(s) 288, 289
BsaJI CCNNGG 6 cut(s) 182, 286, 287, 386, 434, 435
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 288
Bse118I RCCGGY 2 cut(s) 8, 400
Bse1I ACTGG 3 cut(s) 43, 172, 615
Bse3DI GCAATG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 6 cut(s) 182, 286, 287, 386, 434, 435
BseGI GGATG 1 cut(s) 619
BseLI CCNNNNNNNGG 2 cut(s) 167, 288
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 5 cut(s) 34, 462, 492, 522, 552
BseNI ACTGG 3 cut(s) 43, 172, 615
BseRI GAGGAG 1 cut(s) 578
BseXI GCAGC 3 cut(s) 469, 473, 476
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 2 cut(s) 408, 627
BshFI GGCC 2 cut(s) 391, 632
BshNI GGYRCC 1 cut(s) 229
BsiHKAI GWGCWC 2 cut(s) 8, 42
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 3 cut(s) 9, 288, 401
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 167, 288
BsmFI GGGAC 1 cut(s) 304
BsmI GAATGC 1 cut(s) 651
BsnI GGCC 2 cut(s) 391, 632
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 8, 42
Bsp143I GATC 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 408
BspACI CCGC 3 cut(s) 312, 383, 627
BspANI GGCC 2 cut(s) 391, 632
BspCNI CTCAG 5 cut(s) 33, 463, 493, 523, 553
BspFNI CGCG 2 cut(s) 408, 627
BspLI GGNNCC 2 cut(s) 231, 631
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 251
BsrFI RCCGGY 2 cut(s) 8, 400
BsrI ACTGG 3 cut(s) 43, 172, 615
BssAI RCCGGY 2 cut(s) 8, 400
BssECI CCNNGG 6 cut(s) 182, 286, 287, 386, 434, 435
BssMI GATC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 3 cut(s) 14, 216, 283
BstDEI CTNAG 5 cut(s) 20, 471, 501, 531, 561
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 2 cut(s) 408, 627
BstHHI GCGC 3 cut(s) 220, 382, 627
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 5 cut(s) 388, 397, 491, 511, 687
BstNI CCWGG 1 cut(s) 436
BstSCI CCNGG 3 cut(s) 286, 287, 434
BstUI CGCG 2 cut(s) 408, 627
BstV1I GCAGC 3 cut(s) 469, 473, 476
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 2 cut(s) 391, 632
BtgZI GCGATG 1 cut(s) 381
BtsCI GGATG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 165, 450
BtuMI TCGCGA 1 cut(s) 408
Cac8I GCNNGC 3 cut(s) 14, 216, 283
CaiI CAGNNNCTG 1 cut(s) 470
CfoI GCGC 3 cut(s) 220, 382, 627
Cfr10I RCCGGY 2 cut(s) 8, 400
Cfr13I GGNCC 2 cut(s) 186, 630
Cfr9I CCCGGG 1 cut(s) 287
Csp6I GTAC 1 cut(s) 703
CviAII CATG 2 cut(s) 509, 720
CviQI GTAC 1 cut(s) 703
DdeI CTNAG 5 cut(s) 20, 471, 501, 531, 561
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 251, 426
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 2 cut(s) 512, 723
FaiI YATR 8 cut(s) 111, 203, 242, 342, 375, 510, 666, 721
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 1 cut(s) 304
FatI CATG 2 cut(s) 508, 719
FauI CCCGC 1 cut(s) 620
Fnu4HI GCNGC 4 cut(s) 383, 462, 465, 483
FokI GGATG 1 cut(s) 626
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 4 cut(s) 383, 462, 465, 483
FspBI CTAG 1 cut(s) 674
GlaI GCGC 3 cut(s) 219, 381, 626
GluI GCNGC 4 cut(s) 383, 462, 465, 483
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 2 cut(s) 391, 632
HapII CCGG 3 cut(s) 9, 288, 401
HhaI GCGC 3 cut(s) 220, 382, 627
Hin1II CATG 2 cut(s) 512, 723
Hin6I GCGC 3 cut(s) 218, 380, 625
HinP1I GCGC 3 cut(s) 218, 380, 625
HinfI GANTC 2 cut(s) 24, 332
HpaII CCGG 3 cut(s) 9, 288, 401
HphI GGTGA 2 cut(s) 301, 423
Hpy166II GTNNAC 2 cut(s) 189, 448
Hpy188I TCNGA 9 cut(s) 23, 196, 238, 270, 418, 472, 502, 532, 711
Hpy188III TCNNGA 3 cut(s) 407, 489, 518
Hpy8I GTNNAC 2 cut(s) 189, 448
HpyAV CCTTC 4 cut(s) 243, 272, 404, 416
HpyCH4V TGCA 5 cut(s) 85, 214, 244, 249, 461
HpyF10VI GCNNNNNNNGC 5 cut(s) 388, 397, 491, 511, 687
HpyF3I CTNAG 5 cut(s) 20, 471, 501, 531, 561
Hsp92II CATG 2 cut(s) 512, 723
HspAI GCGC 3 cut(s) 218, 380, 625
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 45
Lsp1109I GCAGC 3 cut(s) 469, 473, 476
LweI GCATC 1 cut(s) 523
MaeI CTAG 1 cut(s) 674
MaeIII GTNAC 2 cut(s) 205, 429
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 3 cut(s) 257, 362, 662
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 2 cut(s) 8, 42
MluCI AATT 4 cut(s) 56, 359, 546, 570
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 9 cut(s) 54, 220, 357, 396, 412, 526, 546, 556, 615
MseI TTAA 1 cut(s) 545
MspI CCGG 3 cut(s) 9, 288, 401
MspR9I CCNGG 3 cut(s) 288, 289, 436
Mva1269I GAATGC 1 cut(s) 651
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 2 cut(s) 408, 627
MwoI GCNNNNNNNGC 5 cut(s) 388, 397, 491, 511, 687
NciI CCSGG 2 cut(s) 288, 289
NdeII GATC 1 cut(s) 69
NlaIII CATG 2 cut(s) 512, 723
NlaIV GGNNCC 2 cut(s) 231, 631
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 205, 429
NruI TCGCGA 1 cut(s) 408
PasI CCCWGGG 1 cut(s) 435
PctI GAATGC 1 cut(s) 651
PfeI GAWTC 2 cut(s) 24, 332
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 4 cut(s) 384, 463, 466, 484
PshBI ATTAAT 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 2 cut(s) 231, 631
PspPI GGNCC 2 cut(s) 186, 630
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 1 cut(s) 69
RruI TCGCGA 1 cut(s) 408
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 4 cut(s) 383, 462, 465, 483
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 2 cut(s) 186, 630
ScrFI CCNGG 3 cut(s) 288, 289, 436
SduI GDGCHC 2 cut(s) 8, 42
SfaNI GCATC 1 cut(s) 523
SgrAI CRCCGGYG 1 cut(s) 8
SinI GGWCC 1 cut(s) 186
SmaI CCCGGG 1 cut(s) 289
Sse9I AATT 4 cut(s) 56, 359, 546, 570
SsiI CCGC 3 cut(s) 312, 383, 627
SspMI CTAG 1 cut(s) 674
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 3 cut(s) 286, 287, 434
StyI CCWWGG 1 cut(s) 182
TaqI TCGA 1 cut(s) 27
TasI AATT 4 cut(s) 56, 359, 546, 570
TauI GCSGC 1 cut(s) 385
TfiI GAWTC 2 cut(s) 24, 332
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 2 cut(s) 172, 450
TseFI GTSAC 2 cut(s) 205, 429
TseI GCWGC 3 cut(s) 461, 464, 482
Tsp45I GTSAC 2 cut(s) 205, 429
TspMI CCCGGG 1 cut(s) 287
TspRI CASTG 2 cut(s) 172, 450
Van91I CCANNNNNTGG 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 186
VspI ATTAAT 1 cut(s) 545
XapI RAATTY 1 cut(s) 570
XcmI CCANNNNNNNNNTGG 2 cut(s) 109, 442
XmaI CCCGGG 1 cut(s) 287
XspI CTAG 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.