MD02G1231800.v1.1

Upstream activation factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011758
Physical Location & Seq
Forward (+)
27764533 .. 27767210
2678 bp
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UTR
Exon/CDS
Intron
MD02G1231800.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCGTCTTCCAGTTTGATTAGGGTTTGCAGGGCTGTGACAGCGGCGACGAGGTCCTCCAGTTCCACCGGCGGAGCTGCTCTATTTTCCTCGAAGCCCGCCACCGGTACTGTTCCGAAGCCAAAGGCAAAGGCAAATGCGAAGGCTAAGCCCAAGGAAGCAGGTGTTTCGACCAAGCCCAAACCTACCCTCGGGATTATGAAGCCGATTCCGATCTCTGCAGCTCTGGGAAGCTTCCTCGGCGTCAAGGAGTCCTCCCGCGCCGAAGCTGTCAAGCAGATCTGGGCCCACATCAAGCTCCACAACCTGCAGAATCCTGCCAACAAGAGGGAGATTTACTGTGATGACAAACTAAAGGAGATTTTTGAAGGGAAGGAAAAAGTGGGCTTTCTGGAAATTGGGAAGCTGCTTTCACGCCACTTTGTGAAGGCTGAATGA

Protein Analysis

146

Amino Acids

15.45

Weight (kDa)

10.13

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 68 - 141 1.3e-29 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31760 AT2G35605 AT3G03590 AT3G48600 AT3G48600
fragaria_vesca FvH4_7g08300 FvH4_7g08300
malus_domestica MD02G1231800.v1.1 MD07G1081700.v1.1
prunus_persica Prupe.2G106900_v2.0.a1
pyrus_communis pycom02g20020 pycom07g06500
rosa_chinensis RchiOBHm_Chr1g0340731
rosa_laevigata RLG00000029041
rosa_multiflora Rmu_sc0001211.1_g000138
rosa_roxburghii Rroxscaffold_4G00311370
rosa_samantha Rh1BG144800 Rh1DG176500
rosa_wichuraiana Rw1G014750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 152
Acc36I ACCTGC 2 cut(s) 152, 315
AccII CGCG 1 cut(s) 261
AciI CCGC 4 cut(s) 44, 72, 99, 259
AcyI GRCGYC 2 cut(s) 5, 243
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 104, 191, 327
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 1 cut(s) 368
AluBI AGCT 6 cut(s) 77, 224, 234, 269, 298, 406
AluI AGCT 6 cut(s) 77, 224, 234, 269, 298, 406
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 1 cut(s) 285
ApaI GGGCCC 1 cut(s) 289
ApeKI GCWGC 3 cut(s) 77, 221, 406
AsiGI ACCGGT 1 cut(s) 104
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 54, 285, 286
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 289
BbvI GCAGC 3 cut(s) 64, 233, 393
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BfmI CTRYAG 2 cut(s) 219, 308
BfuAI ACCTGC 2 cut(s) 152, 315
BglII AGATCT 1 cut(s) 279
BisI GCNGC 4 cut(s) 45, 78, 222, 407
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 4 cut(s) 46, 79, 223, 408
Bme18I GGWCC 1 cut(s) 54
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 3 cut(s) 54, 285, 286
BmiI GGNNCC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 43
Bpu1102I GCTNAGC 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 212
BsaHI GRCGYC 2 cut(s) 5, 243
BsaJI CCNNGG 3 cut(s) 153, 190, 238
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 191, 327
Bse118I RCCGGY 2 cut(s) 68, 104
Bse1I ACTGG 2 cut(s) 12, 60
Bse8I GATNNNNATC 1 cut(s) 212
BseDI CCNNGG 3 cut(s) 153, 190, 238
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 3 cut(s) 104, 191, 327
BseNI ACTGG 2 cut(s) 12, 60
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 3 cut(s) 64, 233, 393
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 1 cut(s) 287
BshTI ACCGGT 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 2 cut(s) 69, 105
BslI CCNNNNNNNGG 3 cut(s) 104, 191, 327
BsnI GGCC 1 cut(s) 287
BsoBI CYCGRG 1 cut(s) 191
Bsp120I GGGCCC 1 cut(s) 285
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 213, 279
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 4 cut(s) 44, 72, 99, 259
BspANI GGCC 1 cut(s) 287
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 287
BspMAI CTGCAG 2 cut(s) 223, 312
BspMI ACCTGC 2 cut(s) 152, 315
BsrFI RCCGGY 2 cut(s) 68, 104
BsrI ACTGG 2 cut(s) 12, 60
BssAI RCCGGY 2 cut(s) 68, 104
BssECI CCNNGG 3 cut(s) 153, 190, 238
BssMI GATC 2 cut(s) 213, 279
BssNI GRCGYC 2 cut(s) 5, 243
BssT1I CCWWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 112, 341
BstACI GRCGYC 2 cut(s) 5, 243
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 2 cut(s) 216, 282
BstMBI GATC 2 cut(s) 213, 279
BstMWI GCNNNNNNNGC 2 cut(s) 41, 240
BstSFI CTRYAG 2 cut(s) 219, 308
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 64, 233, 393
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BsuRI GGCC 1 cut(s) 287
BveI ACCTGC 2 cut(s) 152, 315
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 2 cut(s) 68, 104
Cfr13I GGNCC 3 cut(s) 54, 285, 286
CseI GACGC 1 cut(s) 232
Csp6I GTAC 1 cut(s) 108
CspAI ACCGGT 1 cut(s) 104
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 1 cut(s) 147
DpnI GATC 2 cut(s) 215, 281
DpnII GATC 2 cut(s) 213, 279
DraIII CACNNNGTG 1 cut(s) 424
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 153
FaiI YATR 1 cut(s) 200
FauI CCCGC 2 cut(s) 106, 266
Fnu4HI GCNGC 4 cut(s) 45, 78, 222, 407
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 4 cut(s) 45, 78, 222, 407
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 45, 78, 222, 407
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 1 cut(s) 287
HapII CCGG 2 cut(s) 69, 105
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 2 cut(s) 5, 243
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 3 cut(s) 208, 251, 313
HpaII CCGG 2 cut(s) 69, 105
Hpy188I TCNGA 2 cut(s) 117, 213
Hpy188III TCNNGA 2 cut(s) 193, 392
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 4 cut(s) 136, 362, 367, 421
HpyCH4III ACNGT 2 cut(s) 112, 341
HpyCH4V TGCA 3 cut(s) 30, 221, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 240
HpyF3I CTNAG 1 cut(s) 147
Hsp92I GRCGYC 2 cut(s) 5, 243
HspAI GCGC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 213, 279
LmnI GCTCC 2 cut(s) 74, 303
Lsp1109I GCAGC 3 cut(s) 64, 233, 393
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 215, 281
MboI GATC 2 cut(s) 213, 279
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 1 cut(s) 396
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 7 cut(s) 45, 67, 100, 200, 248, 265, 321
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 2 cut(s) 69, 105
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 41, 240
NdeII GATC 2 cut(s) 213, 279
NlaIV GGNNCC 1 cut(s) 287
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 208, 313
PflFI GACNNNGTC 1 cut(s) 52
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 4 cut(s) 46, 79, 223, 408
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 287
PspOMI GGGCCC 1 cut(s) 285
PspPI GGNCC 3 cut(s) 54, 285, 286
PspPPI RGGWCCY 1 cut(s) 54
PstI CTGCAG 2 cut(s) 223, 312
PsuI RGATCY 1 cut(s) 279
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SatI GCNGC 4 cut(s) 45, 78, 222, 407
Sau3AI GATC 2 cut(s) 213, 279
Sau96I GGNCC 3 cut(s) 54, 285, 286
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 1 cut(s) 289
SfcI CTRYAG 2 cut(s) 219, 308
SgrAI CRCCGGYG 1 cut(s) 68
SinI GGWCC 1 cut(s) 54
Sse9I AATT 1 cut(s) 396
SsiI CCGC 4 cut(s) 44, 72, 99, 259
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 112, 341
TaqI TCGA 2 cut(s) 92, 170
TasI AATT 1 cut(s) 396
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 208, 313
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 77, 221, 406
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 215
Tth111I GACNNNGTC 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.