RchiOBHm_Chr1g0340731

Upstream activation factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011758
Physical Location & Seq
Reverse (-)
32288338 .. 32292003
3666 bp
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UTR
Exon/CDS
Intron
PRQ56749

Sequence Viewer

Length: 450 bp
ATGTCGTCCTCCACTGCCATTAGGATTTTCCGAGGCTGCCGTGCTCTCCTGGCGCCGGCCAAGTCCTCCACTTCCACGGCCGGAACCACCGTCAAGGTCGCCGCTGCCGCACCAAAATCGAAGGCGAAGGCCAAGCCCAAGCCCAAGCCCAAAGCGGAAGCCGACGCGGTCAAGCCGAAGAGAAACGTCAACAGGAACGCCGGCATTCTGAAGCCGACTCCGATCTCTCCAGCTCTCGGCAGCTTCCTCGGCGGCGCCCCTGAGTCCTCGCGCGCCGAGGCCGTGAAGCAGATCTGGAGCCACATCAAGCTCCACAACCTGCAGAATCCTGCCAACAAGAGGGAGATCATTTGCGATGACAAGCTGAAGGAGTTGTTTGAAGGGAAGGAGAAGGTCAACTTTTTGGAGATTGGGAAGTTGCTTTCGCGTCATTTTGTGAAGGCTGAGTGA

Protein Analysis

149

Amino Acids

16.02

Weight (kDa)

10.3

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 71 - 145 3.9e-29 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31760 AT2G35605 AT3G03590 AT3G48600 AT3G48600
fragaria_vesca FvH4_7g08300 FvH4_7g08300
malus_domestica MD02G1231800.v1.1 MD07G1081700.v1.1
prunus_persica Prupe.2G106900_v2.0.a1
pyrus_communis pycom02g20020 pycom07g06500
rosa_chinensis RchiOBHm_Chr1g0340731
rosa_laevigata RLG00000029041
rosa_multiflora Rmu_sc0001211.1_g000138
rosa_roxburghii Rroxscaffold_4G00311370
rosa_samantha Rh1BG144800 Rh1DG176500
rosa_wichuraiana Rw1G014750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 2 cut(s) 52, 254
AccII CGCG 4 cut(s) 167, 271, 273, 427
AciI CCGC 5 cut(s) 102, 108, 155, 167, 252
AcoI YGGCCR 2 cut(s) 57, 78
AcuI CTGAAG 2 cut(s) 230, 386
AcyI GRCGYC 2 cut(s) 53, 255
AfiI CCNNNNNNNGG 3 cut(s) 55, 236, 339
AgsI TTSAA 1 cut(s) 380
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 4 cut(s) 233, 243, 310, 364
AluI AGCT 4 cut(s) 233, 243, 310, 364
Alw21I GWGCWC 1 cut(s) 46
AoxI GGCC 4 cut(s) 57, 78, 129, 279
ApeKI GCWGC 3 cut(s) 36, 104, 240
AspLEI GCGC 4 cut(s) 55, 257, 273, 275
BanI GGYRCC 2 cut(s) 52, 254
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 3 cut(s) 23, 91, 252
BceAI ACGGC 3 cut(s) 24, 93, 266
BcgI CGANNNNNNTGC 4 cut(s) 99, 133, 229, 263
BciT130I CCWGG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 320
BfoI RGCGCY 2 cut(s) 56, 258
BfuAI ACCTGC 1 cut(s) 327
BglII AGATCT 1 cut(s) 291
BisI GCNGC 6 cut(s) 37, 102, 105, 108, 241, 253
BlsI GCNGC 6 cut(s) 38, 103, 106, 109, 242, 254
Bme1390I CCNGG 1 cut(s) 50
BmiI GGNNCC 4 cut(s) 54, 85, 256, 299
BmrFI CCNGG 1 cut(s) 50
BpmI CTGGAG 2 cut(s) 213, 316
BsaHI GRCGYC 2 cut(s) 53, 255
BsaJI CCNNGG 4 cut(s) 31, 75, 247, 276
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 236, 339
Bse118I RCCGGY 2 cut(s) 55, 200
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 4 cut(s) 31, 75, 247, 276
BseLI CCNNNNNNNGG 3 cut(s) 55, 236, 339
BseMII CTCAG 2 cut(s) 252, 435
BsePI GCGCGC 1 cut(s) 271
BseX3I CGGCCG 1 cut(s) 78
BseXI GCAGC 3 cut(s) 23, 91, 252
Bsh1236I CGCG 4 cut(s) 167, 271, 273, 427
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 59, 80, 131, 281
BshNI GGYRCC 2 cut(s) 52, 254
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 3 cut(s) 56, 81, 201
BslI CCNNNNNNNGG 3 cut(s) 55, 236, 339
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 4 cut(s) 59, 80, 131, 281
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 3 cut(s) 222, 291, 345
BspACI CCGC 5 cut(s) 102, 108, 155, 167, 252
BspANI GGCC 4 cut(s) 59, 80, 131, 281
BspCNI CTCAG 2 cut(s) 253, 436
BspFNI CGCG 4 cut(s) 167, 271, 273, 427
BspLI GGNNCC 4 cut(s) 54, 85, 256, 299
BspMAI CTGCAG 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 327
BspT107I GGYRCC 2 cut(s) 52, 254
BsrFI RCCGGY 2 cut(s) 55, 200
BssAI RCCGGY 2 cut(s) 55, 200
BssECI CCNNGG 4 cut(s) 31, 75, 247, 276
BssHII GCGCGC 1 cut(s) 271
BssMI GATC 3 cut(s) 222, 291, 345
BssNI GRCGYC 2 cut(s) 53, 255
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 2 cut(s) 53, 255
BstC8I GCNNGC 3 cut(s) 57, 202, 273
BstDEI CTNAG 2 cut(s) 261, 444
BstDSI CCRYGG 1 cut(s) 75
BstFNI CGCG 4 cut(s) 167, 271, 273, 427
BstH2I RGCGCY 2 cut(s) 56, 258
BstHHI GCGC 4 cut(s) 55, 257, 273, 275
BstKTI GATC 3 cut(s) 225, 294, 348
BstMBI GATC 3 cut(s) 222, 291, 345
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 3 cut(s) 50, 107, 249
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 1 cut(s) 320
BstUI CGCG 4 cut(s) 167, 271, 273, 427
BstV1I GCAGC 3 cut(s) 23, 91, 252
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZI CGGCCG 1 cut(s) 78
BsuRI GGCC 4 cut(s) 59, 80, 131, 281
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 369
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 12
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 3 cut(s) 57, 202, 273
CfoI GCGC 4 cut(s) 55, 257, 273, 275
Cfr10I RCCGGY 2 cut(s) 55, 200
CseI GACGC 2 cut(s) 173, 416
DdeI CTNAG 2 cut(s) 261, 444
DinI GGCGCC 2 cut(s) 54, 256
DpnI GATC 3 cut(s) 224, 293, 347
DpnII GATC 3 cut(s) 222, 291, 345
EaeI YGGCCR 2 cut(s) 57, 78
EagI CGGCCG 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 78
Eco57I CTGAAG 2 cut(s) 230, 386
EcoRII CCWGG 1 cut(s) 48
EgeI GGCGCC 2 cut(s) 54, 256
EheI GGCGCC 2 cut(s) 54, 256
FalI AAGNNNNNCTT 2 cut(s) 383, 415
Fnu4HI GCNGC 6 cut(s) 37, 102, 105, 108, 241, 253
Fsp4HI GCNGC 6 cut(s) 37, 102, 105, 108, 241, 253
GlaI GCGC 4 cut(s) 54, 256, 272, 274
GluI GCNGC 6 cut(s) 37, 102, 105, 108, 241, 253
GsuI CTGGAG 2 cut(s) 213, 316
HaeII RGCGCY 2 cut(s) 56, 258
HaeIII GGCC 4 cut(s) 59, 80, 131, 281
HapII CCGG 3 cut(s) 56, 81, 201
HgaI GACGC 2 cut(s) 173, 416
HhaI GCGC 4 cut(s) 55, 257, 273, 275
Hin1I GRCGYC 2 cut(s) 53, 255
Hin6I GCGC 4 cut(s) 53, 255, 271, 273
HinP1I GCGC 4 cut(s) 53, 255, 271, 273
HincII GTYRAC 2 cut(s) 190, 397
HindII GTYRAC 2 cut(s) 190, 397
HinfI GANTC 3 cut(s) 217, 263, 325
HpaII CCGG 3 cut(s) 56, 81, 201
Hpy166II GTNNAC 2 cut(s) 190, 397
Hpy188I TCNGA 3 cut(s) 32, 210, 222
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 2 cut(s) 190, 397
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 7 cut(s) 115, 121, 361, 374, 379, 385, 433
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 107, 249
HpyF3I CTNAG 2 cut(s) 261, 444
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 2 cut(s) 53, 255
HspAI GCGC 4 cut(s) 53, 255, 271, 273
KasI GGCGCC 2 cut(s) 52, 254
KroI GCCGGC 2 cut(s) 55, 200
KroNI GCCGGC 2 cut(s) 57, 202
Kzo9I GATC 3 cut(s) 222, 291, 345
LmnI GCTCC 2 cut(s) 297, 315
Lsp1109I GCAGC 3 cut(s) 23, 91, 252
MaeII ACGT 1 cut(s) 186
MalI GATC 3 cut(s) 224, 293, 347
MboI GATC 3 cut(s) 222, 291, 345
MboII GAAGA 1 cut(s) 190
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 46
Mly113I GGCGCC 2 cut(s) 53, 255
MlyI GAGTC 2 cut(s) 211, 272
MnlI CCTC 7 cut(s) 19, 26, 76, 257, 271, 277, 333
MroNI GCCGGC 2 cut(s) 55, 200
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 3 cut(s) 56, 81, 201
MspR9I CCNGG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 204
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 4 cut(s) 167, 271, 273, 427
MwoI GCNNNNNNNGC 3 cut(s) 50, 107, 249
NaeI GCCGGC 2 cut(s) 57, 202
NarI GGCGCC 2 cut(s) 53, 255
NdeII GATC 3 cut(s) 222, 291, 345
NgoMIV GCCGGC 2 cut(s) 55, 200
NlaIV GGNNCC 4 cut(s) 54, 85, 256, 299
NmeAIII GCCGAG 3 cut(s) 216, 228, 301
PauI GCGCGC 1 cut(s) 271
PctI GAATGC 1 cut(s) 204
PdiI GCCGGC 2 cut(s) 57, 202
PfeI GAWTC 1 cut(s) 325
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 6 cut(s) 38, 103, 106, 109, 242, 254
PleI GAGTC 2 cut(s) 211, 271
PluTI GGCGCC 2 cut(s) 56, 258
PpsI GAGTC 2 cut(s) 211, 271
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 4 cut(s) 54, 85, 256, 299
PstI CTGCAG 1 cut(s) 324
PsuI RGATCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 167
PteI GCGCGC 1 cut(s) 271
SatI GCNGC 6 cut(s) 37, 102, 105, 108, 241, 253
Sau3AI GATC 3 cut(s) 222, 291, 345
SchI GAGTC 2 cut(s) 211, 272
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 46
SetI ASST 8 cut(s) 99, 189, 235, 245, 312, 321, 366, 396
SfcI CTRYAG 1 cut(s) 320
SfoI GGCGCC 2 cut(s) 54, 256
SsiI CCGC 5 cut(s) 102, 108, 155, 167, 252
SspDI GGCGCC 2 cut(s) 52, 254
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 119
TauI GCSGC 3 cut(s) 104, 110, 255
TfiI GAWTC 1 cut(s) 325
TscAI CASTG 1 cut(s) 19
TseI GCWGC 3 cut(s) 36, 104, 240
TspRI CASTG 1 cut(s) 19
Tth111I GACNNNGTC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.