Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0013401

Physical Location & Seq

Genomic Coordinates Reverse (-)

29450495 .. 29461645

Gene Structure 11151 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1244600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGGCAGCACCGAAGCACCGTTCAGGTCGATCAACGCCCCTCAGGGACTCTTCCGAGTCCGAGAAGATGGAGGCCACTGGCCTCTGGGACGCCCTCGACTGGACCATTGAGCCTGTTTCGCGGTCGGTTTCGCGGTCCGTTTCTCAGTCCGTTTCGCATGTAAAATTGGAGTGCTTGCTTGAAGCCGAGCAAGTCCAAGTTGAGGGGTATGGAGTTGTTCTAGTTAATACGGATGAGGCAGGGACCTTGATAGTGACCAATTTTCGTCTTCTATTCCTGAGTGAGGGAACAAGGAATGTTATTGCGCTTGGCACGATACCTTTGACAACAATTGAGAAGTTCACCAAAATTGTCGTAAAGAATCAGTCAACCCCTCGTCAATCTGAAAAGAGTCCATCAAGACGACTTCTTCAGGTCATTGGTAAAGACATGAGGACCATTGTCTTTGGGTTTCGTCCTAAAACAAGGCAGAGACGTGCAATATTTGATGCATTAGTAAGGTGTACAAAGCCAGCAAATCTGTGGGATCTTTATGCTTTTGTGTCTGGACCATCCAAATTTGGTAACACCAGCCCAAAGGTGCGGTTACTAAATGAGTATCTTCGGCTTCTTGGAAAACGGTTCCCACAACCATCGATGGATATGATTGAAGATGGTTCATTCACATTTTCTAATGATTTGTGGAGAATAAGTAGTATAAATTCCAGCTATATACTGTGCCAGAGTTATCCATTTGCCTTGATAGTTCCAAAATCCATAACTGATGAAGAAGTACTCCAAGCTTCCAACTTCCGAGCAAGAGGTCGCTTGCCTGTAGTTACATGGTGTGATTCAAAAACTGGAGCAGTTCTTGCACGGTCATCCCAACCCTTAGTTGGTCTCATGAGGAATATGAGGAGCAACACAGATGAAAAGATTGTTGCTGCACTTTGCACTAAGCTTGCTGGTACAAGAGGTAAACCGAGGAAATTATATATAGCTGATGCAAGACCTCGAAAGAATGCTTTAGCTAATGGTGCTATGGGAGGTGGCTCTGAGTCATCTTCAAATTATCTTCAATCTGAGATAGTTTTCTTTGGGATAGACAACATACATGCGATGAGAGAGAGCTTTGCCCGACTCAGAGACTACTTAGACACTCATGGTGCAGCTTCTTCGGATGGAATGTCATCTTTCTTGAGAAATGGTGGATGGACGTGGGGTGGAGGCAACCTTAGCAGTATGTCTGCTTCAGTGTCAACTTTGGGGGACAGTGGTTGGTTAATACATGTTCAAAGTGTCTTGGCTGGTTCTGCTTGGATTGCTGCTCGTGTTGCTTTGGAGTCGGCATCAGTGCTTGTACATTGTAGTGATGGATGGGACAGGACCACTCAGCTGATTTCACTTGCCAATTTGTTGCTTGATCCATACTATCGTACTTTAACTGGGTTTCAGGCACTCATTGAGAAAGATTGGCTAGCATTTGGTCACCCATTTGCAGATCGAGTTGGCATGCCAGCAGGAAGTGGCAACATGCCTTCTGAATTAACAAGGCAGTACTCTGCTCCGAATCTCCAATCATCACCCAGTCGCCAGTCAGCGTCTCAAGTGCCTTCTCATTCACAGAATTCAAATAACTATTCTCCCATATTTCTGCAGTGGGTTGATTGTGTTTCACAATTACTGCGGATGTATCCTTTTGCTTTTGAGTTTTCTTCGGCATTTCTGGTTGATCTATTGGACTGTGTTCTATCATGTCGCTTTGGAAACTTCTTCTGTAATAGTGAAAGAGAGAGGCAGCTATGTGGTGTACCTGAAGCTTGTGGATGCATGTGGGCATATTTGGCTGATTTACGTGCTTCGCAGGGAAGTTCTCATGTGCACTATAACTACTTTTATGATCCATTAAAACATGATGGTCCACTGTTACCTCCAGCAGCAGCTTTAGCACCAACTCTCTGGCCTCAATTCCATCTTCGTTGGGCTTGTCCCTCAGAGGCTCAAACTGGAGGGATTGAAGCTCAATTTAGGCAAATGGATGTTAAATTCTCTGAACTACAAAAGGAAAAAGAAATGGCAGAAAGGAAAGCTAAAGAAATTGCCACCATGATGGAATCATTAACCGCAGAACTGCAAAATGAGAAGCAAGTTAGCAGCTCAGTTAAGCAGATGGCCACGAGGGCAACCAAGGAAAGTGAGGCCATAAAGCGAGCTGTACAATCACTGGGATGCAGGGTCCACTTTTCAAACAGCGGAGATTGCGCCTTCGACATCGAGAGCAGCCAAATTGAACCTTCGCAGAAGTCACCTTACTATTTAAAAAGAGAAGCGGATGGCACCGTGCAGCGCGATGAGAAATCAGACCTCTCTGTTTCGATCACAGTTATGGCAGATGATGATGCTTCCAGCAACCCAGTCGGCCGAGTATGTGAAACGTTATGCCCGTTACGCATGCGAGATGGGGGCTGCCGGTGGCCAGAAGCTGGTTGTGCTCAGTTGGGTAGCCAATTTCTCGGACTAAAGGCTAACTACGAGGATTTTGATAAACTTTCTATTTATGATAGCTATTTCCAGCCGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010035 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0023052 GO:0030258 GO:0031410 GO:0031668 GO:0031982 GO:0033554 GO:0034593 GO:0035556 GO:0042221 GO:0042578 GO:0042631 GO:0043226 GO:0043227 GO:0043229 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0052629 GO:0052744 GO:0052866 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0080134 GO:0097708 GO:0104004 GO:0106018 GO:1901700 GO:1901701 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Myotub-related

Protein Analysis

854

Amino Acids

94.57

Weight (kDa)

8.26

Isoelectric Point (pI)

58.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myotub-related	PF06602	222 - 593	6.1e-124	Myotubularin-like phosphatase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10550 AT5G04540
fragaria_vesca	FvH4_7g06400
malus_domestica	MD02G1244600.v1.1 MD07G1072600.v1.1
prunus_persica	Prupe.2G090700_v2.0.a1 Prupe.2G090700_v2.0.a1
pyrus_communis	pycom02g21050 pycom07g05570
rosa_chinensis	RchiOBHm_Chr1g0334491
rosa_laevigata	RLG00000029449
rosa_roxburghii	Rroxscaffold_4G00316800
rosa_rugosa	Rorug01G0114300
rosa_samantha	Rh1AG139500 Rh1BG105700 Rh1CG131400 Rh1DG143400
rosa_wichuraiana	Rw1G011500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2315
AccB7I	CCANNNNNTGG	1 cut(s)	2462
AccII	CGCG	3 cut(s)	121, 133, 2328
AciI	CCGC	7 cut(s)	121, 133, 583, 1666, 2103, 2232, 2309
AclI	AACGTT	1 cut(s)	2414
AclWI	GGATC	3 cut(s)	534, 1397, 1874
AcoI	YGGCCR	3 cut(s)	2151, 2398, 2453
AcsI	RAATTY	4 cut(s)	557, 700, 1606, 2024
AcuI	CTGAAG	3 cut(s)	395, 1215, 1815
AcyI	GRCGYC	1 cut(s)	90
AfaI	GTAC	8 cut(s)	505, 774, 949, 1341, 1417, 1538, 1791, 2196
AfiI	CCNNNNNNNGG	5 cut(s)	99, 202, 283, 875, 2462
AflIII	ACRYGT	1 cut(s)	1267
AjiI	CACGTC	2 cut(s)	476, 1197
Alw21I	GWGCWC	2 cut(s)	1863, 2473
Alw26I	GTCTC	4 cut(s)	466, 884, 1119, 1587
Alw44I	GTGCAC	1 cut(s)	1859
AlwI	GGATC	3 cut(s)	534, 1397, 1874
AlwNI	CAGNNNCTG	1 cut(s)	2462
AoxI	GGCC	7 cut(s)	72, 79, 1940, 2151, 2178, 2398, 2453
ApaLI	GTGCAC	1 cut(s)	1859
ApoI	RAATTY	4 cut(s)	557, 700, 1606, 2024
AspLEI	GCGC	3 cut(s)	307, 2243, 2328
AspS9I	GGNCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
AsuHPI	GGTGA	4 cut(s)	334, 1460, 1554, 2277
AsuNHI	GCTAGC	1 cut(s)	1456
AvaII	GGWCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
AxyI	CCTNAGG	1 cut(s)	41
BaeGI	GKGCMC	1 cut(s)	1863
BalI	TGGCCA	2 cut(s)	2153, 2455
BanI	GGYRCC	1 cut(s)	2315
BarI	GAAGNNNNNNTAC	4 cut(s)	1213, 1245, 2273, 2305
BauI	CACGAG	2 cut(s)	1308, 2155
BbsI	GAAGAC	1 cut(s)	260
Bbv12I	GWGCWC	2 cut(s)	1863, 2473
BcgI	CGANNNNNNTGC	4 cut(s)	1137, 1171, 2377, 2411
BciVI	GTATCC	1 cut(s)	1683
BcoDI	GTCTC	4 cut(s)	466, 884, 1119, 1587
BfaI	CTAG	2 cut(s)	221, 1457
BfmI	CTRYAG	2 cut(s)	813, 1634
BfuI	GTATCC	1 cut(s)	1683
BglI	GCCNNNNNGGC	1 cut(s)	2159
BmcAI	AGTACT	2 cut(s)	774, 1538
Bme18I	GGWCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
BmgBI	CACGTC	2 cut(s)	476, 1197
BmgT120I	GGNCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
BmiI	GGNNCC	4 cut(s)	244, 623, 2216, 2317
BmrI	ACTGGG	4 cut(s)	1434, 1560, 2213, 2387
BmsI	GCATC	6 cut(s)	478, 973, 1337, 1796, 2198, 2368
BmtI	GCTAGC	1 cut(s)	1460
BmuI	ACTGGG	4 cut(s)	1434, 1560, 2213, 2387
BoxI	GACNNNNGTC	1 cut(s)	440
BpiI	GAAGAC	1 cut(s)	260
BpmI	CTGGAG	3 cut(s)	861, 1896, 2007
Bpu10I	CCTNAGC	1 cut(s)	1214
BpuEI	CTTGAG	2 cut(s)	1198, 1569
Bsa29I	ATCGAT	1 cut(s)	635
BsaAI	YACGTR	1 cut(s)	1835
BsaHI	GRCGYC	1 cut(s)	90
BsaI	GGTCTC	1 cut(s)	884
BsaJI	CCNNGG	2 cut(s)	962, 2166
BsaXI	ACNNNNNCTCC	2 cut(s)	676, 706
Bsc4I	CCNNNNNNNGG	5 cut(s)	99, 202, 283, 875, 2462
Bse118I	RCCGGY	1 cut(s)	2448
Bse1I	ACTGG	9 cut(s)	82, 104, 844, 1429, 1566, 1573, 1990, 2208, 2393
Bse21I	CCTNAGG	1 cut(s)	41
BseCI	ATCGAT	1 cut(s)	635
BseDI	CCNNGG	2 cut(s)	962, 2166
BseLI	CCNNNNNNNGG	5 cut(s)	99, 202, 283, 875, 2462
BseNI	ACTGG	9 cut(s)	82, 104, 844, 1429, 1566, 1573, 1990, 2208, 2393
BseRI	GAGGAG	1 cut(s)	910
BseSI	GKGCMC	1 cut(s)	1863
BseX3I	CGGCCG	1 cut(s)	2398
BsgI	GTGCAG	3 cut(s)	909, 1167, 2342
Bsh1236I	CGCG	3 cut(s)	121, 133, 2328
Bsh1285I	CGRYCG	2 cut(s)	125, 2401
BshFI	GGCC	7 cut(s)	74, 81, 1942, 2153, 2180, 2400, 2455
BshNI	GGYRCC	1 cut(s)	2315
BshVI	ATCGAT	1 cut(s)	635
BsiEI	CGRYCG	2 cut(s)	125, 2401
BsiHKAI	GWGCWC	2 cut(s)	1863, 2473
BsiSI	CCGG	1 cut(s)	2449
BslFI	GGGAC	6 cut(s)	59, 101, 256, 1262, 1373, 1953
BslI	CCNNNNNNNGG	5 cut(s)	99, 202, 283, 875, 2462
BsmAI	GTCTC	4 cut(s)	466, 884, 1119, 1587
BsmBI	CGTCTC	2 cut(s)	466, 1587
BsmFI	GGGAC	6 cut(s)	59, 101, 256, 1262, 1373, 1953
BsmI	GAATGC	1 cut(s)	1006
BsnI	GGCC	7 cut(s)	74, 81, 1942, 2153, 2180, 2400, 2455
Bso31I	GGTCTC	1 cut(s)	884
Bsp1286I	GDGCHC	2 cut(s)	1863, 2473
Bsp1407I	TGTACA	3 cut(s)	503, 1339, 2194
Bsp143I	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
BspACI	CCGC	7 cut(s)	121, 133, 583, 1666, 2103, 2232, 2309
BspANI	GGCC	7 cut(s)	74, 81, 1942, 2153, 2180, 2400, 2455
BspDI	ATCGAT	1 cut(s)	635
BspFNI	CGCG	3 cut(s)	121, 133, 2328
BspHI	TCATGA	1 cut(s)	882
BspLI	GGNNCC	4 cut(s)	244, 623, 2216, 2317
BspMAI	CTGCAG	1 cut(s)	1638
BspOI	GCTAGC	1 cut(s)	1460
BspPI	GGATC	3 cut(s)	534, 1397, 1874
BspT107I	GGYRCC	1 cut(s)	2315
BspTNI	GGTCTC	1 cut(s)	884
BsrFI	RCCGGY	1 cut(s)	2448
BsrGI	TGTACA	3 cut(s)	503, 1339, 2194
BsrI	ACTGG	9 cut(s)	82, 104, 844, 1429, 1566, 1573, 1990, 2208, 2393
BssAI	RCCGGY	1 cut(s)	2448
BssECI	CCNNGG	2 cut(s)	962, 2166
BssMI	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
BssNI	GRCGYC	1 cut(s)	90
BssSI	CACGAG	2 cut(s)	1308, 2155
BssT1I	CCWWGG	1 cut(s)	2166
Bst2BI	CACGAG	2 cut(s)	1308, 2155
Bst4CI	ACNGT	9 cut(s)	20, 621, 717, 858, 1253, 1724, 1905, 2320, 2362
Bst6I	CTCTTC	1 cut(s)	55
BstACI	GRCGYC	1 cut(s)	90
BstAPI	GCANNNNNTGC	1 cut(s)	851
BstAUI	TGTACA	3 cut(s)	503, 1339, 2194
BstBAI	YACGTR	1 cut(s)	1835
BstC8I	GCNNGC	9 cut(s)	176, 513, 809, 942, 1458, 1493, 1497, 2190, 2432
BstEII	GGTNACC	1 cut(s)	1466
BstENI	CCTNNNNNAGG	1 cut(s)	281
BstFNI	CGCG	3 cut(s)	121, 133, 2328
BstHHI	GCGC	3 cut(s)	307, 2243, 2328
BstKTI	GATC	7 cut(s)	32, 529, 1405, 1483, 1714, 1882, 2358
BstMAI	GTCTC	4 cut(s)	466, 884, 1119, 1587
BstMBI	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
BstMCI	CGRYCG	2 cut(s)	125, 2401
BstNSI	RCATGY	7 cut(s)	161, 1097, 1271, 1495, 1516, 1813, 2434
BstPAI	GACNNNNGTC	1 cut(s)	440
BstPI	GGTNACC	1 cut(s)	1466
BstSFI	CTRYAG	2 cut(s)	813, 1634
BstSLI	GKGCMC	1 cut(s)	1863
BstUI	CGCG	3 cut(s)	121, 133, 2328
BstV2I	GAAGAC	1 cut(s)	260
BstX2I	RGATCY	1 cut(s)	526
BstXI	CCANNNNNNTGG	2 cut(s)	1938, 2089
BstYI	RGATCY	1 cut(s)	526
BstZI	CGGCCG	1 cut(s)	2398
Bsu15I	ATCGAT	1 cut(s)	635
Bsu36I	CCTNAGG	1 cut(s)	41
BsuI	GTATCC	1 cut(s)	1683
BsuRI	GGCC	7 cut(s)	74, 81, 1942, 2153, 2180, 2400, 2455
BsuTUI	ATCGAT	1 cut(s)	635
BtgZI	GCGATG	2 cut(s)	1112, 2343
BtrI	CACGTC	2 cut(s)	476, 1197
BtsI	GCAGTG	1 cut(s)	1643
BtsIMutI	CAGTG	7 cut(s)	75, 1239, 1258, 1338, 1643, 1901, 2201
Cac8I	GCNNGC	9 cut(s)	176, 513, 809, 942, 1458, 1493, 1497, 2190, 2432
CaiI	CAGNNNCTG	1 cut(s)	2462
CciI	TCATGA	1 cut(s)	882
CfoI	GCGC	3 cut(s)	307, 2243, 2328
Cfr10I	RCCGGY	1 cut(s)	2448
Cfr13I	GGNCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
ClaI	ATCGAT	1 cut(s)	635
CpoI	CGGWCCG	1 cut(s)	135
CseI	GACGC	2 cut(s)	98, 1569
Csp6I	GTAC	8 cut(s)	504, 773, 948, 1340, 1416, 1537, 1790, 2195
CspI	CGGWCCG	1 cut(s)	135
CviQI	GTAC	8 cut(s)	504, 773, 948, 1340, 1416, 1537, 1790, 2195
DpnI	GATC	7 cut(s)	31, 528, 1404, 1482, 1713, 1881, 2357
DpnII	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
DraI	TTTAAA	1 cut(s)	2298
EaeI	YGGCCR	3 cut(s)	2151, 2398, 2453
EagI	CGGCCG	1 cut(s)	2398
Eam1104I	CTCTTC	1 cut(s)	55
EarI	CTCTTC	1 cut(s)	55
EclXI	CGGCCG	1 cut(s)	2398
Eco130I	CCWWGG	1 cut(s)	2166
Eco31I	GGTCTC	1 cut(s)	884
Eco47I	GGWCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
Eco52I	CGGCCG	1 cut(s)	2398
Eco57I	CTGAAG	3 cut(s)	395, 1215, 1815
Eco81I	CCTNAGG	1 cut(s)	41
Eco91I	GGTNACC	1 cut(s)	1466
EcoNI	CCTNNNNNAGG	1 cut(s)	281
EcoO109I	RGGNCCY	1 cut(s)	243
EcoO65I	GGTNACC	1 cut(s)	1466
EcoRI	GAATTC	1 cut(s)	1606
EcoT14I	CCWWGG	1 cut(s)	2166
EcoT22I	ATGCAT	2 cut(s)	493, 1811
ErhI	CCWWGG	1 cut(s)	2166
Esp3I	CGTCTC	2 cut(s)	466, 1587
FaqI	GGGAC	6 cut(s)	59, 101, 256, 1262, 1373, 1953
FspBI	CTAG	2 cut(s)	221, 1457
GlaI	GCGC	3 cut(s)	306, 2242, 2327
GsuI	CTGGAG	3 cut(s)	861, 1896, 2007
HaeIII	GGCC	7 cut(s)	74, 81, 1942, 2153, 2180, 2400, 2455
HapII	CCGG	1 cut(s)	2449
HgaI	GACGC	2 cut(s)	98, 1569
HhaI	GCGC	3 cut(s)	307, 2243, 2328
Hin1I	GRCGYC	1 cut(s)	90
Hin6I	GCGC	3 cut(s)	305, 2241, 2326
HinP1I	GCGC	3 cut(s)	305, 2241, 2326
HincII	GTYRAC	2 cut(s)	369, 1239
HindII	GTYRAC	2 cut(s)	369, 1239
HindIII	AAGCTT	3 cut(s)	780, 938, 1797
HpaII	CCGG	1 cut(s)	2449
HphI	GGTGA	4 cut(s)	334, 1460, 1554, 2277
Hpy166II	GTNNAC	9 cut(s)	342, 369, 504, 959, 1239, 1790, 1861, 1901, 2218
Hpy188III	TCNNGA	6 cut(s)	277, 399, 546, 883, 1177, 2254
Hpy8I	GTNNAC	9 cut(s)	342, 369, 504, 959, 1239, 1790, 1861, 1901, 2218
HpyAV	CCTTC	4 cut(s)	1527, 1602, 2254, 2283
HpyCH4III	ACNGT	9 cut(s)	20, 621, 717, 858, 1253, 1724, 1905, 2320, 2362
HpyCH4IV	ACGT	4 cut(s)	475, 1196, 1834, 2414
HpySE526I	ACGT	4 cut(s)	475, 1196, 1834, 2414
Hsp92I	GRCGYC	1 cut(s)	90
HspAI	GCGC	3 cut(s)	305, 2241, 2326
Kzo9I	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
LmnI	GCTCC	3 cut(s)	842, 897, 1549
LweI	GCATC	6 cut(s)	478, 973, 1337, 1796, 2198, 2368
MaeI	CTAG	2 cut(s)	221, 1457
MaeII	ACGT	4 cut(s)	475, 1196, 1834, 2414
MaeIII	GTNAC	8 cut(s)	253, 563, 585, 817, 1466, 1905, 2283, 2424
MalI	GATC	7 cut(s)	31, 528, 1404, 1482, 1713, 1881, 2357
MboI	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
MfeI	CAATTG	1 cut(s)	330
MflI	RGATCY	1 cut(s)	526
MhlI	GDGCHC	2 cut(s)	1863, 2473
MlsI	TGGCCA	2 cut(s)	2153, 2455
MluNI	TGGCCA	2 cut(s)	2153, 2455
MlyI	GAGTC	6 cut(s)	41, 65, 400, 1046, 1113, 1331
MmeI	TCCRAC	1 cut(s)	810
Mox20I	TGGCCA	2 cut(s)	2153, 2455
Mph1103I	ATGCAT	2 cut(s)	493, 1811
MscI	TGGCCA	2 cut(s)	2153, 2455
MseI	TTAA	9 cut(s)	225, 1262, 1421, 1526, 1886, 2022, 2099, 2142, 2297
MslI	CAYNNNNRTG	4 cut(s)	1347, 2087, 2206, 2363
Msp20I	TGGCCA	2 cut(s)	2153, 2455
MspA1I	CMGCKG	2 cut(s)	1375, 2232
MspI	CCGG	1 cut(s)	2449
MunI	CAATTG	1 cut(s)	330
Mva1269I	GAATGC	1 cut(s)	1006
MvnI	CGCG	3 cut(s)	121, 133, 2328
NdeII	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
NheI	GCTAGC	1 cut(s)	1456
NlaIV	GGNNCC	4 cut(s)	244, 623, 2216, 2317
NmeAIII	GCCGAG	2 cut(s)	211, 2426
NmuCI	GTSAC	3 cut(s)	253, 1466, 2283
NsiI	ATGCAT	2 cut(s)	493, 1811
NspI	RCATGY	7 cut(s)	161, 1097, 1271, 1495, 1516, 1813, 2434
PaeI	GCATGC	2 cut(s)	1495, 2434
PagI	TCATGA	1 cut(s)	882
PciI	ACATGT	1 cut(s)	1267
PcsI	WCGNNNNNNNCGW	1 cut(s)	2420
PctI	GAATGC	1 cut(s)	1006
PfeI	GAWTC	4 cut(s)	361, 830, 1549, 2093
PflMI	CCANNNNNTGG	1 cut(s)	2462
PleI	GAGTC	6 cut(s)	41, 64, 399, 1045, 1113, 1330
PpsI	GAGTC	6 cut(s)	41, 64, 399, 1045, 1113, 1330
Ppu21I	YACGTR	1 cut(s)	1835
PpuMI	RGGWCCY	1 cut(s)	243
PscI	ACATGT	1 cut(s)	1267
PshAI	GACNNNNGTC	1 cut(s)	440
Psp1406I	AACGTT	1 cut(s)	2414
Psp5II	RGGWCCY	1 cut(s)	243
PspEI	GGTNACC	1 cut(s)	1466
PspN4I	GGNNCC	4 cut(s)	244, 623, 2216, 2317
PspPI	GGNCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
PspPPI	RGGWCCY	1 cut(s)	243
PstI	CTGCAG	1 cut(s)	1638
PstNI	CAGNNNCTG	1 cut(s)	2462
PsuI	RGATCY	1 cut(s)	526
PvuII	CAGCTG	1 cut(s)	1375
RsaI	GTAC	8 cut(s)	505, 774, 949, 1341, 1417, 1538, 1791, 2196
RsaNI	GTAC	8 cut(s)	504, 773, 948, 1340, 1416, 1537, 1790, 2195
RseI	CAYNNNNRTG	4 cut(s)	1347, 2087, 2206, 2363
Rsr2I	CGGWCCG	1 cut(s)	135
RsrII	CGGWCCG	1 cut(s)	135
SaqAI	TTAA	9 cut(s)	225, 1262, 1421, 1526, 1886, 2022, 2099, 2142, 2297
Sau3AI	GATC	7 cut(s)	29, 526, 1402, 1480, 1711, 1879, 2355
Sau96I	GGNCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
ScaI	AGTACT	2 cut(s)	774, 1538
SchI	GAGTC	6 cut(s)	41, 65, 400, 1046, 1113, 1331
SduI	GDGCHC	2 cut(s)	1863, 2473
SfaNI	GCATC	6 cut(s)	478, 973, 1337, 1796, 2198, 2368
SfcI	CTRYAG	2 cut(s)	813, 1634
SinI	GGWCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
SmiMI	CAYNNNNRTG	4 cut(s)	1347, 2087, 2206, 2363
SmlI	CTYRAG	2 cut(s)	1177, 1584
SmoI	CTYRAG	2 cut(s)	1177, 1584
SphI	GCATGC	2 cut(s)	1495, 2434
SsiI	CCGC	7 cut(s)	121, 133, 583, 1666, 2103, 2232, 2309
SspI	AATATT	1 cut(s)	483
SspMI	CTAG	2 cut(s)	221, 1457
StyI	CCWWGG	1 cut(s)	2166
TaaI	ACNGT	9 cut(s)	20, 621, 717, 858, 1253, 1724, 1905, 2320, 2362
TaiI	ACGT	4 cut(s)	478, 1199, 1837, 2417
TaqI	TCGA	8 cut(s)	28, 96, 635, 994, 1483, 2247, 2253, 2354
TatI	WGTACW	5 cut(s)	503, 772, 1339, 1536, 2194
TfiI	GAWTC	4 cut(s)	361, 830, 1549, 2093
Tru1I	TTAA	9 cut(s)	225, 1262, 1421, 1526, 1886, 2022, 2099, 2142, 2297
Tru9I	TTAA	9 cut(s)	225, 1262, 1421, 1526, 1886, 2022, 2099, 2142, 2297
TscAI	CASTG	7 cut(s)	82, 1239, 1258, 1338, 1643, 1908, 2208
TseFI	GTSAC	3 cut(s)	253, 1466, 2283
Tsp45I	GTSAC	3 cut(s)	253, 1466, 2283
TspDTI	ATGAA	3 cut(s)	648, 780, 924
TspGWI	ACGGA	3 cut(s)	127, 139, 245
TspRI	CASTG	7 cut(s)	82, 1239, 1258, 1338, 1643, 1908, 2208
Van91I	CCANNNNNTGG	1 cut(s)	2462
VneI	GTGCAC	1 cut(s)	1859
VpaK11BI	GGWCC	8 cut(s)	102, 135, 243, 435, 548, 1365, 1898, 2215
XagI	CCTNNNNNAGG	1 cut(s)	281
XapI	RAATTY	4 cut(s)	557, 700, 1606, 2024
XceI	RCATGY	7 cut(s)	161, 1097, 1271, 1495, 1516, 1813, 2434
XcmI	CCANNNNNNNNNTGG	2 cut(s)	519, 872
XspI	CTAG	2 cut(s)	221, 1457
ZrmI	AGTACT	2 cut(s)	774, 1538
Zsp2I	ATGCAT	2 cut(s)	493, 1811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.