Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013401

Physical Location & Seq

Genomic Coordinates Forward (+)

6863092 .. 6874656

Gene Structure 11565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1072600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGGCAGCACCGAAGCACAGCTCAGGTCGATCGACGTCTTTCATGGACTCTTCCGAGTCTGAGAAGATGGAGGCCACCGGCAGCTGGGACGCCCTCGACTGGACCATTGAGCCGGTTTCGCGGTCCGTTTCTCGGCCCGTTTCGCATGTGAAATTGGAGTGCCTGCTTGAAGCCGAGCAAGTCCAAGTTGAGGGGTATGGAGTTGTTCTTGTTAATACAGATGAGGCGGGCACCTTGATAGTGACCAATTATCGTCTTCTATTCCTGAGTGAGGGAACTAGGAATGTTATTGCACTTGGCACGATACCGTTGGCAACAATCGAGAAGTTCACCAAAATTGTTGTAAAGAATCAGCCAACCCCTCGTCAATCCGAAAAGATTCCATCAAGAAGACTTCTTCAGGTCATTGGTAAAGACATGAGGACCATTGTCTTTGGGTTTCGTCCTAAAACAAGGCAGAGACGTGCAATATTTGATGCATTATCAAGTTGTACAAAGCCAACAAGTCTGTGGGATCTTTATGCTTTTGTTTCTGGACCTTCCAAATTTGGTAACACCAACCCAAAGGTGCGGTTACTAAATGAGTATTTTCGGCTTCTTGGGAAAGGGTCCTTACGTGCATCTATGAATATGATTGAAGATGGGTCATTCACATTTTCTAATGATTTGTGGAGGATAAGTAGCATAAATTCCAGCTATATCATGTGCCAGAGTTATCCATTTGCCTTGATGGTTCCAAAATCCATAACTGATGAAGAAGTGCTTCAAGCTTCCAACTTCCGAGCAAGAGGTCGCTTGCCTGTAGTTACATGGTATCATCCAAAAACGGGAGCAGTTCTTGCACGGTCATCCCAACCCTTAGTTGGTCTCATGATGAATATGAGGAGTAACACAGATGAAAAGATTGTTGCTGCACTTTGCACTCAACTTGCTGGTACAAGAGTCAAACAGAGGAAGTTATATATAGCCGATGCAAGGCCTCGAAAAAATGCTCTAGCTAATGGTGCTATGGGAGGTGGCTCTGAGTCATCTTCACATTATTCTCAATCTGAGATAGTTTTCTTTGGGATAGACAACATACATGCAATGAGAGAGAGCTTTGCCAGACTCAGAGACTATTTAGACACTCATGGTGCAGCTTCATCGGATGGAATGTCATCTTTCTTGAGACATGGTGGATGGACTTGGGGTGGAGGTAACCTAAGCAGTATGTCTGCTTCAGTGTCAACACTGGGGGACAGTGGTTGGTTAATACATGTTCAAAGTGTCTTGGCTGGTTCTGCTTGGATTGCTGCTCGTGTTGCTTTGGAGTCGGCATCAGTGCTTGTACATTGTAGTGATGGATGGGACAGAACCACTCAGCTGATTTCACTTGCCAATTTGTTGCTTGATCCATACTATCGGACTTTAACTGGGTTTCAGGCACTGGTTGAGAAAGATTGGTTAGCATTTGGTCACCCATTCGCAGATCGAGTTGGCATGCCAGCTGTAATGGGAAGTGGCAACATGCCTTCTGAATTGACGAGGCAGGCCTCTGCTCCGGTTCTCCAGTCATCACCCAGTCGCCATTCATCAGCGTCTCAGGCGCCTACTTCTCATGCACAGAATTCAAATGACTATTCTCCCATATTTCTGCAGTGGGTTGATTGTGTTTCACAATTGTTGCGGATGTATCCTTTTGCTTTTGAGTTTTCTTCGGCATTTCTGGTGGATCTATTGGACTGTGTTCTATCGTGTCGCTTTGGAAACTTCTTATGTAATAGTGAAAGGGAGAGGCAGCAATGTGGTGTACCCGATGCTTGTGGATGCATGTGGGTATATTTTGCTGATTTACGTGCTTCGCAGGGAAGTTCTCATGTGCACTATAACTACTTATATGATCCCTTGAAACACGATGGTCCACTGTTACCTCCAGCAGCAGCTTTAGCACCAACTCTTTGGCCTCAATTCCATCTTCGTTGGGCTTGTTCCTCAGAGGCTCAAGTTGGAGGGGTTGAAGCTCAATTTAGGCAAATGGATGTTAAGTTCTCTGAACTACAAAAGGAAAAAGAAATGGCAGAAAGGAAAGCTAAAGAAATCACCACCTTAATGGAATCATTAACCGCAGAATTGCAAAATGAGAAGCAAGTTAGCAGCTCAGCTAAGCAGATGGCCAAGAGGGCAAGCAGGGAAAGTGACGCCATAAAGCGAGCTGTACAGTCACTGGGATGCAAGGTCCACTTTTCAAACAGTGGAGATTGCACCGTTGGCATCGAAAGCAGCCAAATTGCATCTTCGCAGAAATTACCTTATTATTTAAAAAGAGAAGCGGATGGCACCATGCAGCGTGATGAGAAATTAGACCTCTCTCTTTCAATCACGGTTATGGCAGATGATGATGCTTCCATCAACCCAATTGGCCGAGTATGTGAAACGTTATGCCCGTTAGGCATGCGAGATGGGGGCTGCCGGTGGCCGGATGCTGGTTGTGCTCAATTGGGTAGCCAATTTCTCGGACTAAAGGTTAACTACGAGGCATTTGATAAACTTTCTATTTATGATAGCTATTTCCAGCCGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010035 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0023052 GO:0030258 GO:0031410 GO:0031668 GO:0031982 GO:0033554 GO:0034593 GO:0035556 GO:0042221 GO:0042578 GO:0042631 GO:0043226 GO:0043227 GO:0043229 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0052629 GO:0052744 GO:0052866 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0080134 GO:0097708 GO:0104004 GO:0106018 GO:1901700 GO:1901701 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Myotub-related

Protein Analysis

854

Amino Acids

94.23

Weight (kDa)

8.15

Isoelectric Point (pI)

50.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myotub-related	PF06602	218 - 593	6.5e-126	Myotubularin-like phosphatase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10550 AT5G04540
fragaria_vesca	FvH4_7g06400
malus_domestica	MD02G1244600.v1.1 MD07G1072600.v1.1
prunus_persica	Prupe.2G090700_v2.0.a1 Prupe.2G090700_v2.0.a1
pyrus_communis	pycom02g21050 pycom07g05570
rosa_chinensis	RchiOBHm_Chr1g0334491
rosa_laevigata	RLG00000029449
rosa_roxburghii	Rroxscaffold_4G00316800
rosa_rugosa	Rorug01G0114300
rosa_samantha	Rh1AG139500 Rh1BG105700 Rh1CG131400 Rh1DG143400
rosa_wichuraiana	Rw1G011500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	38
AccB1I	GGYRCC	3 cut(s)	230, 1585, 2315
AccII	CGCG	1 cut(s)	121
AciI	CCGC	6 cut(s)	121, 227, 571, 1666, 2103, 2309
AclI	AACGTT	1 cut(s)	2414
AclWI	GGATC	4 cut(s)	522, 1385, 1719, 1874
AcoI	YGGCCR	3 cut(s)	2151, 2398, 2453
AcsI	RAATTY	3 cut(s)	545, 688, 1606
AcuI	CTGAAG	2 cut(s)	383, 1203
AcyI	GRCGYC	4 cut(s)	35, 90, 1586, 2178
AfaI	GTAC	5 cut(s)	493, 937, 1329, 1791, 2196
AflIII	ACRYGT	1 cut(s)	1255
AgsI	TTSAA	9 cut(s)	170, 638, 767, 1262, 1611, 1888, 1997, 2226, 2355
AjiI	CACGTC	1 cut(s)	464
AloI	GAACNNNNNNTCC	2 cut(s)	2009, 2041
Alw21I	GWGCWC	2 cut(s)	1863, 2473
Alw26I	GTCTC	5 cut(s)	454, 872, 1107, 1162, 1584
Alw44I	GTGCAC	1 cut(s)	1859
AlwI	GGATC	4 cut(s)	522, 1385, 1719, 1874
AlwNI	CAGNNNCTG	2 cut(s)	1426, 2203
AoxI	GGCC	8 cut(s)	72, 134, 977, 1530, 1940, 2151, 2398, 2453
ApaLI	GTGCAC	1 cut(s)	1859
ApoI	RAATTY	3 cut(s)	545, 688, 1606
Asp700I	GAANNNNTTC	2 cut(s)	378, 762
AspLEI	GCGC	1 cut(s)	1588
AspS9I	GGNCC	8 cut(s)	102, 123, 135, 423, 536, 609, 1898, 2215
AsuHPI	GGTGA	4 cut(s)	322, 1448, 1548, 2071
AvaII	GGWCC	7 cut(s)	102, 123, 423, 536, 609, 1898, 2215
BaeGI	GKGCMC	2 cut(s)	233, 1863
BaeI	ACNNNNGTAYC	2 cut(s)	927, 960
BalI	TGGCCA	1 cut(s)	2153
BanI	GGYRCC	3 cut(s)	230, 1585, 2315
BarI	GAAGNNNNNNTAC	2 cut(s)	1201, 1233
BauI	CACGAG	1 cut(s)	1296
BbsI	GAAGAC	2 cut(s)	248, 397
Bbv12I	GWGCWC	2 cut(s)	1863, 2473
BcgI	CGANNNNNNTGC	2 cut(s)	1125, 1159
BciVI	GTATCC	1 cut(s)	1683
BcoDI	GTCTC	5 cut(s)	454, 872, 1107, 1162, 1584
BfaI	CTAG	2 cut(s)	279, 995
BfmI	CTRYAG	2 cut(s)	801, 1634
BfoI	RGCGCY	1 cut(s)	1589
BfuI	GTATCC	1 cut(s)	1683
BglI	GCCNNNNNGGC	2 cut(s)	2159, 2427
BlpI	GCTNAGC	2 cut(s)	2137, 2142
Bme18I	GGWCC	7 cut(s)	102, 123, 423, 536, 609, 1898, 2215
BmgBI	CACGTC	1 cut(s)	464
BmgT120I	GGNCC	8 cut(s)	102, 123, 135, 423, 536, 609, 1898, 2215
BmiI	GGNNCC	5 cut(s)	232, 610, 735, 1587, 2317
BmrI	ACTGGG	4 cut(s)	1241, 1422, 1554, 2213
BmuI	ACTGGG	4 cut(s)	1241, 1422, 1554, 2213
BoxI	GACNNNNGTC	1 cut(s)	428
BpiI	GAAGAC	2 cut(s)	248, 397
BpmI	CTGGAG	2 cut(s)	1532, 1896
Bpu10I	CCTNAGC	2 cut(s)	22, 1202
Bpu1102I	GCTNAGC	2 cut(s)	2137, 2142
BpuEI	CTTGAG	2 cut(s)	1186, 1965
BsaAI	YACGTR	2 cut(s)	617, 1835
BsaHI	GRCGYC	4 cut(s)	35, 90, 1586, 2178
BsaI	GGTCTC	1 cut(s)	872
BsaWI	WCCGGW	1 cut(s)	1540
BsaXI	ACNNNNNCTCC	2 cut(s)	664, 694
Bse118I	RCCGGY	3 cut(s)	77, 112, 2448
Bse1I	ACTGG	7 cut(s)	104, 1236, 1417, 1431, 1549, 1560, 2208
Bse3DI	GCAATG	2 cut(s)	1092, 1787
BseMI	GCAATG	2 cut(s)	1092, 1787
BseNI	ACTGG	7 cut(s)	104, 1236, 1417, 1431, 1549, 1560, 2208
BseRI	GAGGAG	1 cut(s)	898
BseSI	GKGCMC	2 cut(s)	233, 1863
BseYI	CCCAGC	1 cut(s)	84
BsgI	GTGCAG	2 cut(s)	897, 1155
Bsh1236I	CGCG	1 cut(s)	121
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	8 cut(s)	74, 136, 979, 1532, 1942, 2153, 2400, 2455
BshNI	GGYRCC	3 cut(s)	230, 1585, 2315
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	2 cut(s)	1863, 2473
BsiSI	CCGG	5 cut(s)	78, 113, 1541, 2449, 2456
BslFI	GGGAC	3 cut(s)	101, 1250, 1361
BsmAI	GTCTC	5 cut(s)	454, 872, 1107, 1162, 1584
BsmBI	CGTCTC	2 cut(s)	454, 1584
BsmFI	GGGAC	3 cut(s)	101, 1250, 1361
BsnI	GGCC	8 cut(s)	74, 136, 979, 1532, 1942, 2153, 2400, 2455
Bso31I	GGTCTC	1 cut(s)	872
Bsp1286I	GDGCHC	3 cut(s)	233, 1863, 2473
Bsp1407I	TGTACA	3 cut(s)	491, 1327, 2194
Bsp143I	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
Bsp1720I	GCTNAGC	2 cut(s)	2137, 2142
BspACI	CCGC	6 cut(s)	121, 227, 571, 1666, 2103, 2309
BspANI	GGCC	8 cut(s)	74, 136, 979, 1532, 1942, 2153, 2400, 2455
BspFNI	CGCG	1 cut(s)	121
BspHI	TCATGA	1 cut(s)	870
BspLI	GGNNCC	5 cut(s)	232, 610, 735, 1587, 2317
BspMAI	CTGCAG	1 cut(s)	1638
BspPI	GGATC	4 cut(s)	522, 1385, 1719, 1874
BspT107I	GGYRCC	3 cut(s)	230, 1585, 2315
BspTNI	GGTCTC	1 cut(s)	872
BsrDI	GCAATG	2 cut(s)	1092, 1787
BsrFI	RCCGGY	3 cut(s)	77, 112, 2448
BsrGI	TGTACA	3 cut(s)	491, 1327, 2194
BsrI	ACTGG	7 cut(s)	104, 1236, 1417, 1431, 1549, 1560, 2208
BssAI	RCCGGY	3 cut(s)	77, 112, 2448
BssMI	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
BssNI	GRCGYC	4 cut(s)	35, 90, 1586, 2178
BssSI	CACGAG	1 cut(s)	1296
Bst2BI	CACGAG	1 cut(s)	1296
Bst4CI	ACNGT	9 cut(s)	309, 846, 1241, 1724, 1905, 2199, 2231, 2245, 2362
Bst6I	CTCTTC	1 cut(s)	55
BstACI	GRCGYC	4 cut(s)	35, 90, 1586, 2178
BstAPI	GCANNNNNTGC	1 cut(s)	839
BstAUI	TGTACA	3 cut(s)	491, 1327, 2194
BstBAI	YACGTR	2 cut(s)	617, 1835
BstC8I	GCNNGC	9 cut(s)	164, 229, 797, 1481, 1485, 1530, 2164, 2190, 2432
BstEII	GGTNACC	2 cut(s)	1196, 1454
BstENI	CCTNNNNNAGG	1 cut(s)	269
BstFNI	CGCG	1 cut(s)	121
BstH2I	RGCGCY	1 cut(s)	1589
BstHHI	GCGC	1 cut(s)	1588
BstKTI	GATC	6 cut(s)	32, 517, 1393, 1471, 1714, 1882
BstMAI	GTCTC	5 cut(s)	454, 872, 1107, 1162, 1584
BstMBI	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
BstMCI	CGRYCG	1 cut(s)	32
BstNSI	RCATGY	7 cut(s)	149, 1085, 1259, 1483, 1510, 1813, 2434
BstPAI	GACNNNNGTC	1 cut(s)	428
BstPI	GGTNACC	2 cut(s)	1196, 1454
BstSFI	CTRYAG	2 cut(s)	801, 1634
BstSLI	GKGCMC	2 cut(s)	233, 1863
BstUI	CGCG	1 cut(s)	121
BstV2I	GAAGAC	2 cut(s)	248, 397
BstX2I	RGATCY	2 cut(s)	514, 1711
BstXI	CCANNNNNNTGG	2 cut(s)	1938, 2089
BstYI	RGATCY	2 cut(s)	514, 1711
BsuI	GTATCC	1 cut(s)	1683
BsuRI	GGCC	8 cut(s)	74, 136, 979, 1532, 1942, 2153, 2400, 2455
BtrI	CACGTC	1 cut(s)	464
BtsI	GCAGTG	1 cut(s)	1643
BtsIMutI	CAGTG	9 cut(s)	1227, 1229, 1246, 1326, 1424, 1643, 1901, 2201, 2236
Cac8I	GCNNGC	9 cut(s)	164, 229, 797, 1481, 1485, 1530, 2164, 2190, 2432
CaiI	CAGNNNCTG	2 cut(s)	1426, 2203
CciI	TCATGA	1 cut(s)	870
CfoI	GCGC	1 cut(s)	1588
Cfr10I	RCCGGY	3 cut(s)	77, 112, 2448
Cfr13I	GGNCC	8 cut(s)	102, 123, 135, 423, 536, 609, 1898, 2215
CpoI	CGGWCCG	1 cut(s)	123
CseI	GACGC	3 cut(s)	98, 1566, 2186
Csp6I	GTAC	5 cut(s)	492, 936, 1328, 1790, 2195
CspI	CGGWCCG	1 cut(s)	123
CviQI	GTAC	5 cut(s)	492, 936, 1328, 1790, 2195
DinI	GGCGCC	1 cut(s)	1587
DpnI	GATC	6 cut(s)	31, 516, 1392, 1470, 1713, 1881
DpnII	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
DraI	TTTAAA	1 cut(s)	2298
EaeI	YGGCCR	3 cut(s)	2151, 2398, 2453
Eam1104I	CTCTTC	1 cut(s)	55
EarI	CTCTTC	1 cut(s)	55
Eco147I	AGGCCT	2 cut(s)	979, 1532
Eco31I	GGTCTC	1 cut(s)	872
Eco47I	GGWCC	7 cut(s)	102, 123, 423, 536, 609, 1898, 2215
Eco57I	CTGAAG	2 cut(s)	383, 1203
Eco91I	GGTNACC	2 cut(s)	1196, 1454
EcoNI	CCTNNNNNAGG	1 cut(s)	269
EcoO109I	RGGNCCY	1 cut(s)	609
EcoO65I	GGTNACC	2 cut(s)	1196, 1454
EcoRI	GAATTC	1 cut(s)	1606
EcoT22I	ATGCAT	2 cut(s)	481, 1811
EgeI	GGCGCC	1 cut(s)	1587
EheI	GGCGCC	1 cut(s)	1587
Esp3I	CGTCTC	2 cut(s)	454, 1584
FalI	AAGNNNNNCTT	4 cut(s)	747, 779, 2204, 2236
FaqI	GGGAC	3 cut(s)	101, 1250, 1361
FauI	CCCGC	1 cut(s)	220
FspBI	CTAG	2 cut(s)	279, 995
GlaI	GCGC	1 cut(s)	1587
GsaI	CCCAGC	1 cut(s)	88
GsuI	CTGGAG	2 cut(s)	1532, 1896
HaeII	RGCGCY	1 cut(s)	1589
HaeIII	GGCC	8 cut(s)	74, 136, 979, 1532, 1942, 2153, 2400, 2455
HapII	CCGG	5 cut(s)	78, 113, 1541, 2449, 2456
HgaI	GACGC	3 cut(s)	98, 1566, 2186
HhaI	GCGC	1 cut(s)	1588
Hin1I	GRCGYC	4 cut(s)	35, 90, 1586, 2178
Hin6I	GCGC	1 cut(s)	1586
HinP1I	GCGC	1 cut(s)	1586
HincII	GTYRAC	2 cut(s)	1227, 2506
HindII	GTYRAC	2 cut(s)	1227, 2506
HindIII	AAGCTT	1 cut(s)	768
HinfI	GANTC	9 cut(s)	47, 56, 349, 379, 942, 1025, 1107, 1310, 2093
HpaI	GTTAAC	1 cut(s)	2506
HpaII	CCGG	5 cut(s)	78, 113, 1541, 2449, 2456
HphI	GGTGA	4 cut(s)	322, 1448, 1548, 2071
Hpy166II	GTNNAC	7 cut(s)	330, 1227, 1790, 1861, 1901, 2218, 2506
Hpy188III	TCNNGA	6 cut(s)	265, 322, 387, 534, 871, 1165
Hpy8I	GTNNAC	7 cut(s)	330, 1227, 1790, 1861, 1901, 2218, 2506
Hpy99I	CGWCG	1 cut(s)	37
HpyAV	CCTTC	2 cut(s)	549, 1521
HpyCH4III	ACNGT	9 cut(s)	309, 846, 1241, 1724, 1905, 2199, 2231, 2245, 2362
HpyCH4IV	ACGT	5 cut(s)	35, 463, 616, 1834, 2414
HpySE526I	ACGT	5 cut(s)	35, 463, 616, 1834, 2414
Hsp92I	GRCGYC	4 cut(s)	35, 90, 1586, 2178
HspAI	GCGC	1 cut(s)	1586
KasI	GGCGCC	1 cut(s)	1585
KspAI	GTTAAC	1 cut(s)	2506
Kzo9I	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
LmnI	GCTCC	2 cut(s)	830, 1543
MaeI	CTAG	2 cut(s)	279, 995
MaeII	ACGT	5 cut(s)	35, 463, 616, 1834, 2414
MalI	GATC	6 cut(s)	31, 516, 1392, 1470, 1713, 1881
MboI	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
MfeI	CAATTG	3 cut(s)	1658, 2394, 2474
MflI	RGATCY	2 cut(s)	514, 1711
MhlI	GDGCHC	3 cut(s)	233, 1863, 2473
MlsI	TGGCCA	1 cut(s)	2153
MluNI	TGGCCA	1 cut(s)	2153
Mly113I	GGCGCC	1 cut(s)	1586
MlyI	GAGTC	6 cut(s)	41, 65, 951, 1034, 1101, 1319
MmeI	TCCRAC	2 cut(s)	798, 1966
Mox20I	TGGCCA	1 cut(s)	2153
Mph1103I	ATGCAT	2 cut(s)	481, 1811
MroXI	GAANNNNTTC	2 cut(s)	378, 762
MscI	TGGCCA	1 cut(s)	2153
MseI	TTAA	8 cut(s)	213, 1250, 1409, 2022, 2087, 2099, 2297, 2505
MslI	CAYNNNNRTG	4 cut(s)	1335, 2087, 2206, 2363
Msp20I	TGGCCA	1 cut(s)	2153
MspA1I	CMGCKG	3 cut(s)	84, 1363, 1487
MspI	CCGG	5 cut(s)	78, 113, 1541, 2449, 2456
MunI	CAATTG	3 cut(s)	1658, 2394, 2474
MvnI	CGCG	1 cut(s)	121
NarI	GGCGCC	1 cut(s)	1586
NdeII	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
NlaIV	GGNNCC	5 cut(s)	232, 610, 735, 1587, 2317
NmeAIII	GCCGAG	3 cut(s)	112, 199, 2426
NmuCI	GTSAC	4 cut(s)	241, 1454, 2174, 2199
NsiI	ATGCAT	2 cut(s)	481, 1811
NspI	RCATGY	7 cut(s)	149, 1085, 1259, 1483, 1510, 1813, 2434
PaeI	GCATGC	2 cut(s)	1483, 2434
PagI	TCATGA	1 cut(s)	870
PceI	AGGCCT	2 cut(s)	979, 1532
PciI	ACATGT	1 cut(s)	1255
PcsI	WCGNNNNNNNCGW	1 cut(s)	2420
PdmI	GAANNNNTTC	2 cut(s)	378, 762
PfeI	GAWTC	3 cut(s)	349, 379, 2093
Ple19I	CGATCG	1 cut(s)	32
PleI	GAGTC	6 cut(s)	41, 64, 950, 1033, 1101, 1318
PluTI	GGCGCC	1 cut(s)	1589
PpsI	GAGTC	6 cut(s)	41, 64, 950, 1033, 1101, 1318
Ppu21I	YACGTR	2 cut(s)	617, 1835
PpuMI	RGGWCCY	1 cut(s)	609
PscI	ACATGT	1 cut(s)	1255
PshAI	GACNNNNGTC	1 cut(s)	428
Psp1406I	AACGTT	1 cut(s)	2414
Psp5II	RGGWCCY	1 cut(s)	609
PspEI	GGTNACC	2 cut(s)	1196, 1454
PspFI	CCCAGC	1 cut(s)	84
PspN4I	GGNNCC	5 cut(s)	232, 610, 735, 1587, 2317
PspPI	GGNCC	8 cut(s)	102, 123, 135, 423, 536, 609, 1898, 2215
PspPPI	RGGWCCY	1 cut(s)	609
PstI	CTGCAG	1 cut(s)	1638
PstNI	CAGNNNCTG	2 cut(s)	1426, 2203
PsuI	RGATCY	2 cut(s)	514, 1711
PvuI	CGATCG	1 cut(s)	32
PvuII	CAGCTG	3 cut(s)	84, 1363, 1487
RsaI	GTAC	5 cut(s)	493, 937, 1329, 1791, 2196
RsaNI	GTAC	5 cut(s)	492, 936, 1328, 1790, 2195
RseI	CAYNNNNRTG	4 cut(s)	1335, 2087, 2206, 2363
Rsr2I	CGGWCCG	1 cut(s)	123
RsrII	CGGWCCG	1 cut(s)	123
SaqAI	TTAA	8 cut(s)	213, 1250, 1409, 2022, 2087, 2099, 2297, 2505
Sau3AI	GATC	6 cut(s)	29, 514, 1390, 1468, 1711, 1879
Sau96I	GGNCC	8 cut(s)	102, 123, 135, 423, 536, 609, 1898, 2215
SchI	GAGTC	6 cut(s)	41, 65, 951, 1034, 1101, 1319
SduI	GDGCHC	3 cut(s)	233, 1863, 2473
SfcI	CTRYAG	2 cut(s)	801, 1634
SfoI	GGCGCC	1 cut(s)	1587
SinI	GGWCC	7 cut(s)	102, 123, 423, 536, 609, 1898, 2215
SmiMI	CAYNNNNRTG	4 cut(s)	1335, 2087, 2206, 2363
SmlI	CTYRAG	2 cut(s)	1165, 1980
SmoI	CTYRAG	2 cut(s)	1165, 1980
SphI	GCATGC	2 cut(s)	1483, 2434
SseBI	AGGCCT	2 cut(s)	979, 1532
SsiI	CCGC	6 cut(s)	121, 227, 571, 1666, 2103, 2309
SspDI	GGCGCC	1 cut(s)	1585
SspI	AATATT	1 cut(s)	471
SspMI	CTAG	2 cut(s)	279, 995
StuI	AGGCCT	2 cut(s)	979, 1532
TaaI	ACNGT	9 cut(s)	309, 846, 1241, 1724, 1905, 2199, 2231, 2245, 2362
TaiI	ACGT	5 cut(s)	38, 466, 619, 1837, 2417
TaqI	TCGA	7 cut(s)	28, 32, 96, 321, 982, 1471, 2253
TatI	WGTACW	3 cut(s)	491, 1327, 2194
TfiI	GAWTC	3 cut(s)	349, 379, 2093
Tru1I	TTAA	8 cut(s)	213, 1250, 1409, 2022, 2087, 2099, 2297, 2505
Tru9I	TTAA	8 cut(s)	213, 1250, 1409, 2022, 2087, 2099, 2297, 2505
TscAI	CASTG	9 cut(s)	1227, 1236, 1246, 1326, 1431, 1643, 1908, 2208, 2236
TseFI	GTSAC	4 cut(s)	241, 1454, 2174, 2199
Tsp45I	GTSAC	4 cut(s)	241, 1454, 2174, 2199
TspDTI	ATGAA	7 cut(s)	31, 641, 768, 890, 912, 1131, 1560
TspGWI	ACGGA	1 cut(s)	115
TspRI	CASTG	9 cut(s)	1227, 1236, 1246, 1326, 1431, 1643, 1908, 2208, 2236
VneI	GTGCAC	1 cut(s)	1859
VpaK11BI	GGWCC	7 cut(s)	102, 123, 423, 536, 609, 1898, 2215
XagI	CCTNNNNNAGG	1 cut(s)	269
XapI	RAATTY	3 cut(s)	545, 688, 1606
XceI	RCATGY	7 cut(s)	149, 1085, 1259, 1483, 1510, 1813, 2434
XcmI	CCANNNNNNNNNTGG	2 cut(s)	507, 860
XmnI	GAANNNNTTC	2 cut(s)	378, 762
XspI	CTAG	2 cut(s)	279, 995
ZraI	GACGTC	1 cut(s)	36
Zsp2I	ATGCAT	2 cut(s)	481, 1811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.