MD02G1255000.v1.1

Enhancer of polycomb-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008000
Physical Location & Seq
Reverse (-)
30668128 .. 30672669
4542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1255000.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGCCATCGGTGGAGATGAGGCGGACCACGAGGGTTTTCGGGATGGCGATGGCCAAGGGCGGTGTGGACGGGGCTCGGGTTTTGCGGTCCGGCCGCCGCCTCTGGCCCGAATCGTCCGAGTCCAAGATGGTGAGGGCCCACCGTGGCGATGAGGACTGGCTTAAAGTCATGAAAAGCCATGCCAGTGATTCTGTTGTTTCTTTAAAGAACAAGAGATGGCCCGGGAACAACCACATTCGGAGTCCCAAGCAACACGCCGTGGTTTTGGAAGTTCCATTGGTGAAGAAGCTTGAAAGTAATGAGCTTTTGGCTGATGAAGTTAAAGAGAAGACGATGTATGGGATTGTGTATACCCGAAAGAGGAAGCGCGCCACCGCCAATGTGGAGAACGATGGTGGTTCCGATGATAGAATGTATGGGCGGAGATTTGTTCGGAGGCAGAGGAGGAAGGTGAACGGGGAGTTGGATTCCCATGTGGGTTTTGTGTCCCGGGAAGTGTTTTGTGTTTCGATTGAGTCTTTGTGCAGTAGGAGATACTGGGCTGCCCCTTTTTTGTATTCGGTTTTGGTGTACATGACGAGGGCCAGTCTGGGATTGACTGAGCTTTCTGATTTCCTTGCATCAGAGCCAATTGGTAGCTTTTTTTCATCCTGTGGCATTCAGTTCTCGCGGGTTCGATCTTGTACTAGGAGCTCTGGAATTTGCAAGTTATTTGGGTTGAAACAGTTTATCCCATTGTTTTCTGTGGACTTTTCTGCAGTTCCGTTTTGTTTTATGGACATGCATACAATCATCCATCTCAGATATAAATGTCAGCCAGCTATAAATAATCGGATTGATGGGCATGAAAGGGATGATGACTGTGACGAACAAGTTGATTGTATTGAGTGCACGCCGGTTTCGCATTCTAGTGCAAGAGTTCCCAATTTAGCTTATAGAAACACACAGTATAGGAATGGTTTAAATTCACGTGGTGTTCAGAAGAGGAGGAGTTCGTTGAGGGGAAGGCGATCTAGAAGTCCGTTGCTGGTTTCTTTACGAAAGCCCAATGGAACCTTAGTTTCTGAGCTAACCGATATTAGGAAGAACGGCCTCCCTTTTTCCTCTGTAGCAACTATTAATAAGCGTAGGAAGACATTTCCAACTTCTCCTGTAGGAAACCTGAAAGAAGACAGTTCAACCATGGAGAGATTTACACGGGATTTAGATTCTGCAAGCTGCTCGGCGAATATACTGATCACTGAAGGGGACAAATGTTTTAAAGAAGATGGAGCAACTGTCATGTTAGAAATCTCTTCATCTGGAGAATGGCTTCTTGTGGTCAAGAAAGAAGGATTAACCCGATACACCCACAAGGCAGAGAAGGTAATGAGGCCCTGCTCTAAAAACCGTCTTACGAATGTTATAATGTGGTCTGCGGATAATGGGGATAATTGGAAGCTAGAATTTCCCAATCGATGTGATTGGTATATATTCAAGGACCTTTACAAGGAGTGTTCTGATCGGTTTGTGCCATCTCCAGCTCTCAAGTTCATCCCTGTGCCCGGTGTGTATGAAGTCCCAGGATATGCGGATAGTCACTGCACTTCTTTCCACAGACCAGATTCATACATATCTCTGAATGATGACGAGTTGTCTAGAGCCATGGCAAAGAGAAATGCAAATTATGACATGGACTCTGATGATGAGGGGTGGCTGAAGAAGTTCAACAATGACCTTGCTGAGGATAAACTTGATGACCTAGTTTCCGAGGATAAGTTTGAATTAATTGTTGATGCGATTGAGAAGGCCTTTTATTGTAGACCATATGATTTCTCTGATGAGAATCCAGCTGCTAATCTTTGTCTGGATATGGGGAGGAGGGAAGTAATAGAGGCTGTATATAGTTATTGGATGGATAAACGGAAGCAGAAGCAATTATCATCGCTGCTTAGGGTGTTCCAGGCGAATCAATCGAAGAGATCTCTACTAGACCCAAAGCCTATTCTACGCAAGAGAAGATCATTCAAGAGGCAACCAAGTCAATCTGGTAGAGGAAAACAACCAATTTTCTTCCAAGCTATGGCTGTGGAACAAGATGCGATGCAAGAGCAGAATGATGTGGTTAGAGTTGAAGAAGCGAAAGATGCAGCCGAGAGATCTGTGGAATTTGCTATCCGCAAACGGCAACGGGCTCAGTTAGTCATGCAGAACGCAGATCTAGTAACTTATAAAGCGACGATGGCATTCAGAATCGCTGAAGCTGCCCTGGTTCTCGGATCACCGGATGCCGCTGCTGATTATGTACTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

765

Amino Acids

87.25

Weight (kDa)

9.2

Isoelectric Point (pI)

60.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 510 - 596 5.8e-12 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1406, 2211
AccB7I CCANNNNNTGG 1 cut(s) 2062
AccI GTMKAC 2 cut(s) 350, 1801
AccII CGCG 2 cut(s) 369, 670
AclWI GGATC 1 cut(s) 2266
AcoI YGGCCR 2 cut(s) 51, 91
AcsI RAATTY 4 cut(s) 699, 964, 1445, 2147
AcuI CTGAAG 3 cut(s) 1263, 1718, 2259
AcvI CACGTG 1 cut(s) 971
AdeI CACNNNGTG 2 cut(s) 259, 974
AfaI GTAC 3 cut(s) 572, 685, 2286
AfiI CCNNNNNNNGG 6 cut(s) 360, 1080, 1489, 1544, 1723, 2062
AgsI TTSAA 8 cut(s) 293, 721, 1179, 1477, 1708, 1763, 2008, 2114
AhdI GACNNNNNGTC 1 cut(s) 1633
AjnI CCWGG 3 cut(s) 1561, 1941, 2247
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
Alw21I GWGCWC 2 cut(s) 695, 893
Alw44I GTGCAC 1 cut(s) 889
AlwI GGATC 1 cut(s) 2266
Ama87I CYCGRG 3 cut(s) 75, 221, 489
AoxI GGCC 9 cut(s) 51, 91, 104, 135, 218, 582, 1090, 1373, 1788
ApaI GGGCCC 1 cut(s) 139
ApaLI GTGCAC 1 cut(s) 889
ApeKI GCWGC 7 cut(s) 542, 1218, 1832, 1927, 2129, 2243, 2273
ApoI RAATTY 4 cut(s) 699, 964, 1445, 2147
AseI ATTAAT 2 cut(s) 1119, 1766
Asp700I GAANNNNTTC 2 cut(s) 1311, 1703
AspLEI GCGC 2 cut(s) 369, 371
AspS9I GGNCC 9 cut(s) 24, 87, 105, 135, 136, 219, 582, 1374, 1480
AsuC2I CCSGG 5 cut(s) 222, 223, 490, 491, 1545
AsuHPI GGTGA 4 cut(s) 142, 292, 463, 2253
AvaI CYCGRG 3 cut(s) 75, 221, 489
AvaII GGWCC 3 cut(s) 24, 87, 1480
BaeGI GKGCMC 3 cut(s) 139, 893, 1545
BalI TGGCCA 1 cut(s) 53
BanII GRGCYC 4 cut(s) 76, 139, 695, 2176
BauI CACGAG 1 cut(s) 28
BbrPI CACGTG 1 cut(s) 971
BbsI GAAGAC 3 cut(s) 335, 1139, 1176
Bbv12I GWGCWC 2 cut(s) 695, 893
BbvCI CCTCAGC 1 cut(s) 1722
BbvI GCAGC 7 cut(s) 529, 1205, 1819, 1914, 2141, 2230, 2260
BceAI ACGGC 3 cut(s) 242, 1105, 2180
BcgI CGANNNNNNTGC 2 cut(s) 1203, 1237
BciT130I CCWGG 3 cut(s) 1563, 1943, 2249
BclI TGATCA 1 cut(s) 1236
BcnI CCSGG 5 cut(s) 222, 223, 490, 491, 1545
BfaI CTAG 8 cut(s) 687, 909, 1014, 1442, 1638, 1742, 1970, 2201
BfmI CTRYAG 3 cut(s) 756, 1107, 1152
BglII AGATCT 3 cut(s) 1961, 2138, 2197
Bme1390I CCNGG 8 cut(s) 222, 223, 490, 491, 1545, 1563, 1943, 2249
Bme18I GGWCC 3 cut(s) 24, 87, 1480
BmeRI GACNNNNNGTC 1 cut(s) 1633
BmeT110I CYCGRG 3 cut(s) 75, 221, 489
BmgT120I GGNCC 9 cut(s) 24, 87, 105, 135, 136, 219, 582, 1374, 1480
BmiI GGNNCC 3 cut(s) 137, 400, 1054
BmrFI CCNGG 8 cut(s) 222, 223, 490, 491, 1545, 1563, 1943, 2249
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 6 cut(s) 629, 1765, 2068, 2073, 2116, 2257
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 3 cut(s) 335, 1139, 1176
BpmI CTGGAG 2 cut(s) 1323, 1503
Bpu10I CCTNAGC 2 cut(s) 1722, 1931
BpuEI CTTGAG 1 cut(s) 1511
BpuMI CCSGG 5 cut(s) 222, 223, 490, 491, 1545
Bsa29I ATCGAT 1 cut(s) 1456
BsaAI YACGTR 1 cut(s) 971
BsaBI GATNNNNATC 1 cut(s) 1824
BsaWI WCCGGW 1 cut(s) 2263
BsaXI ACNNNNNCTCC 2 cut(s) 1263, 1293
Bsc4I CCNNNNNNNGG 6 cut(s) 360, 1080, 1489, 1544, 1723, 2062
Bse118I RCCGGY 1 cut(s) 895
Bse1I ACTGG 5 cut(s) 161, 183, 542, 585, 2292
Bse8I GATNNNNATC 1 cut(s) 1824
BseBI CCWGG 3 cut(s) 1563, 1943, 2249
BseCI ATCGAT 1 cut(s) 1456
BseGI GGATG 7 cut(s) 48, 647, 792, 859, 1533, 1899, 2272
BseJI GATNNNNATC 1 cut(s) 1824
BseLI CCNNNNNNNGG 6 cut(s) 360, 1080, 1489, 1544, 1723, 2062
BseMII CTCAG 5 cut(s) 591, 814, 1056, 1713, 2189
BseNI ACTGG 5 cut(s) 161, 183, 542, 585, 2292
BsePI GCGCGC 1 cut(s) 367
BseRI GAGGAG 4 cut(s) 457, 1000, 1003, 1873
BseSI GKGCMC 3 cut(s) 139, 893, 1545
BseX3I CGGCCG 1 cut(s) 91
BseXI GCAGC 7 cut(s) 529, 1205, 1819, 1914, 2141, 2230, 2260
BsgI GTGCAG 2 cut(s) 544, 1567
Bsh1236I CGCG 2 cut(s) 369, 670
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 9 cut(s) 53, 93, 106, 137, 220, 584, 1092, 1375, 1790
BshVI ATCGAT 1 cut(s) 1456
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 695, 893
BsiHKCI CYCGRG 3 cut(s) 75, 221, 489
BsiSI CCGG 6 cut(s) 90, 222, 490, 896, 1545, 2264
BslFI GGGAC 4 cut(s) 228, 472, 1262, 1544
BslI CCNNNNNNNGG 6 cut(s) 360, 1080, 1489, 1544, 1723, 2062
BsmFI GGGAC 4 cut(s) 228, 472, 1262, 1544
BsmI GAATGC 3 cut(s) 657, 904, 2225
BsnI GGCC 9 cut(s) 53, 93, 106, 137, 220, 584, 1092, 1375, 1790
BsoBI CYCGRG 3 cut(s) 75, 221, 489
Bsp120I GGGCCC 1 cut(s) 135
Bsp1286I GDGCHC 6 cut(s) 76, 139, 695, 893, 1545, 2176
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
Bsp19I CCATGG 2 cut(s) 1182, 1644
BspANI GGCC 9 cut(s) 53, 93, 106, 137, 220, 584, 1092, 1375, 1790
BspCNI CTCAG 5 cut(s) 592, 813, 1057, 1714, 2188
BspDI ATCGAT 1 cut(s) 1456
BspFNI CGCG 2 cut(s) 369, 670
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 3 cut(s) 137, 400, 1054
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 1 cut(s) 2266
BsrFI RCCGGY 1 cut(s) 895
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 5 cut(s) 161, 183, 542, 585, 2292
BssAI RCCGGY 1 cut(s) 895
BssHII GCGCGC 1 cut(s) 367
BssMI GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
BssNAI GTATAC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 28
BssT1I CCWWGG 3 cut(s) 54, 1182, 1644
Bst1107I GTATAC 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 28
Bst2UI CCWGG 3 cut(s) 1563, 1943, 2249
Bst4CI ACNGT 7 cut(s) 143, 726, 863, 948, 1175, 1279, 1391
Bst6I CTCTTC 3 cut(s) 977, 1300, 1952
BstAUI TGTACA 1 cut(s) 570
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 4 cut(s) 369, 819, 893, 1216
BstDEI CTNAG 7 cut(s) 600, 800, 1057, 1065, 1722, 1931, 2175
BstDSI CCRYGG 4 cut(s) 142, 258, 1182, 1644
BstENI CCTNNNNNAGG 2 cut(s) 1487, 1721
BstF5I GGATG 7 cut(s) 48, 647, 792, 859, 1533, 1899, 2272
BstFNI CGCG 2 cut(s) 369, 670
BstHHI GCGC 2 cut(s) 369, 371
BstKTI GATC 9 cut(s) 680, 1013, 1239, 1504, 1964, 2003, 2141, 2200, 2261
BstMBI GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 4 cut(s) 901, 2126, 2222, 2243
BstNI CCWGG 3 cut(s) 1563, 1943, 2249
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 8 cut(s) 220, 221, 488, 489, 1543, 1561, 1941, 2247
BstSFI CTRYAG 3 cut(s) 756, 1107, 1152
BstSLI GKGCMC 3 cut(s) 139, 893, 1545
BstUI CGCG 2 cut(s) 369, 670
BstV1I GCAGC 7 cut(s) 529, 1205, 1819, 1914, 2141, 2230, 2260
BstV2I GAAGAC 3 cut(s) 335, 1139, 1176
BstX2I RGATCY 3 cut(s) 1961, 2138, 2197
BstYI RGATCY 3 cut(s) 1961, 2138, 2197
BstZ17I GTATAC 1 cut(s) 351
BstZI CGGCCG 1 cut(s) 91
Bsu15I ATCGAT 1 cut(s) 1456
BsuRI GGCC 9 cut(s) 53, 93, 106, 137, 220, 584, 1092, 1375, 1790
BsuTUI ATCGAT 1 cut(s) 1456
BtgI CCRYGG 4 cut(s) 142, 258, 1182, 1644
BtgZI GCGATG 4 cut(s) 62, 162, 1908, 2096
BtsCI GGATG 7 cut(s) 48, 647, 792, 859, 1533, 1899, 2272
BtsI GCAGTG 1 cut(s) 1579
BtsIMutI CAGTG 3 cut(s) 190, 1239, 1579
Cac8I GCNNGC 4 cut(s) 369, 819, 893, 1216
CciI TCATGA 1 cut(s) 168
CfoI GCGC 2 cut(s) 369, 371
Cfr10I RCCGGY 1 cut(s) 895
Cfr13I GGNCC 9 cut(s) 24, 87, 105, 135, 136, 219, 582, 1374, 1480
Cfr9I CCCGGG 2 cut(s) 221, 489
ClaI ATCGAT 1 cut(s) 1456
CpoI CGGWCCG 1 cut(s) 87
Csp6I GTAC 3 cut(s) 571, 684, 2285
CspI CGGWCCG 1 cut(s) 87
CviQI GTAC 3 cut(s) 571, 684, 2285
DdeI CTNAG 7 cut(s) 600, 800, 1057, 1065, 1722, 1931, 2175
DpnI GATC 9 cut(s) 679, 1012, 1238, 1503, 1963, 2002, 2140, 2199, 2260
DpnII GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
DraI TTTAAA 3 cut(s) 204, 963, 1261
DraIII CACNNNGTG 2 cut(s) 259, 974
DriI GACNNNNNGTC 1 cut(s) 1633
EaeI YGGCCR 2 cut(s) 51, 91
EagI CGGCCG 1 cut(s) 91
Eam1104I CTCTTC 3 cut(s) 977, 1300, 1952
Eam1105I GACNNNNNGTC 1 cut(s) 1633
EarI CTCTTC 3 cut(s) 977, 1300, 1952
EciI GGCGGA 2 cut(s) 37, 436
Ecl136II GAGCTC 1 cut(s) 693
EclXI CGGCCG 1 cut(s) 91
Eco130I CCWWGG 3 cut(s) 54, 1182, 1644
Eco147I AGGCCT 1 cut(s) 1790
Eco24I GRGCYC 4 cut(s) 76, 139, 695, 2176
Eco47I GGWCC 3 cut(s) 24, 87, 1480
Eco52I CGGCCG 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 693
Eco57I CTGAAG 3 cut(s) 1263, 1718, 2259
Eco72I CACGTG 1 cut(s) 971
Eco88I CYCGRG 3 cut(s) 75, 221, 489
EcoICRI GAGCTC 1 cut(s) 693
EcoNI CCTNNNNNAGG 2 cut(s) 1487, 1721
EcoO109I RGGNCCY 3 cut(s) 135, 1374, 1480
EcoRII CCWGG 3 cut(s) 1561, 1941, 2247
EcoT14I CCWWGG 3 cut(s) 54, 1182, 1644
EcoT22I ATGCAT 1 cut(s) 786
EcoT38I GRGCYC 4 cut(s) 76, 139, 695, 2176
ErhI CCWWGG 3 cut(s) 54, 1182, 1644
FaqI GGGAC 4 cut(s) 228, 472, 1262, 1544
FauI CCCGC 1 cut(s) 663
FauNDI CATATG 1 cut(s) 1807
FbaI TGATCA 1 cut(s) 1236
FblI GTMKAC 2 cut(s) 350, 1801
FokI GGATG 7 cut(s) 55, 634, 779, 866, 1520, 1906, 2279
FriOI GRGCYC 4 cut(s) 76, 139, 695, 2176
FspBI CTAG 8 cut(s) 687, 909, 1014, 1442, 1638, 1742, 1970, 2201
GlaI GCGC 2 cut(s) 368, 370
GsuI CTGGAG 2 cut(s) 1323, 1503
HaeIII GGCC 9 cut(s) 53, 93, 106, 137, 220, 584, 1092, 1375, 1790
HapII CCGG 6 cut(s) 90, 222, 490, 896, 1545, 2264
HhaI GCGC 2 cut(s) 369, 371
Hin6I GCGC 2 cut(s) 367, 369
HinP1I GCGC 2 cut(s) 367, 369
HindIII AAGCTT 1 cut(s) 287
HpaII CCGG 6 cut(s) 90, 222, 490, 896, 1545, 2264
HphI GGTGA 4 cut(s) 142, 292, 463, 2253
Hpy166II GTNNAC 7 cut(s) 67, 351, 454, 571, 748, 891, 1802
Hpy188III TCNNGA 9 cut(s) 40, 169, 696, 1014, 1302, 1324, 1638, 1847, 2008
Hpy8I GTNNAC 7 cut(s) 67, 351, 454, 571, 748, 891, 1802
Hpy99I CGWCG 1 cut(s) 2221
HpyAV CCTTC 6 cut(s) 442, 999, 1238, 1325, 1357, 1780
HpyCH4III ACNGT 7 cut(s) 143, 726, 863, 948, 1175, 1279, 1391
HpyCH4IV ACGT 1 cut(s) 970
HpyF10VI GCNNNNNNNGC 4 cut(s) 901, 2126, 2222, 2243
HpyF3I CTNAG 7 cut(s) 600, 800, 1057, 1065, 1722, 1931, 2175
HpySE526I ACGT 1 cut(s) 970
HspAI GCGC 2 cut(s) 367, 369
Ksp22I TGATCA 1 cut(s) 1236
Kzo9I GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
LmnI GCTCC 2 cut(s) 690, 1271
Lsp1109I GCAGC 7 cut(s) 529, 1205, 1819, 1914, 2141, 2230, 2260
LweI GCATC 6 cut(s) 629, 1765, 2068, 2073, 2116, 2257
MaeI CTAG 8 cut(s) 687, 909, 1014, 1442, 1638, 1742, 1970, 2201
MaeII ACGT 1 cut(s) 970
MaeIII GTNAC 3 cut(s) 863, 1577, 2203
MalI GATC 9 cut(s) 679, 1012, 1238, 1503, 1963, 2002, 2140, 2199, 2260
MboI GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 3 cut(s) 1961, 2138, 2197
MhlI GDGCHC 6 cut(s) 76, 139, 695, 893, 1545, 2176
MlsI TGGCCA 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 4 cut(s) 128, 250, 524, 1670
MmeI TCCRAC 2 cut(s) 444, 1166
Mox20I TGGCCA 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 786
MroXI GAANNNNTTC 2 cut(s) 1311, 1703
MscI TGGCCA 1 cut(s) 53
MseI TTAA 8 cut(s) 162, 203, 321, 962, 1119, 1260, 1337, 1766
MslI CAYNNNNRTG 3 cut(s) 183, 909, 1538
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 2 cut(s) 1832, 2273
MspI CCGG 6 cut(s) 90, 222, 490, 896, 1545, 2264
MspR9I CCNGG 8 cut(s) 222, 223, 490, 491, 1545, 1563, 1943, 2249
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 3 cut(s) 657, 904, 2225
MvaI CCWGG 3 cut(s) 1563, 1943, 2249
MvnI CGCG 2 cut(s) 369, 670
MwoI GCNNNNNNNGC 4 cut(s) 901, 2126, 2222, 2243
NciI CCSGG 5 cut(s) 222, 223, 490, 491, 1545
NcoI CCATGG 2 cut(s) 1182, 1644
NdeI CATATG 1 cut(s) 1807
NdeII GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
NlaIV GGNNCC 3 cut(s) 137, 400, 1054
NmeAIII GCCGAG 2 cut(s) 1202, 2158
NmuCI GTSAC 2 cut(s) 863, 1577
NsiI ATGCAT 1 cut(s) 786
NspI RCATGY 1 cut(s) 784
PagI TCATGA 1 cut(s) 168
PauI GCGCGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 1790
PctI GAATGC 3 cut(s) 657, 904, 2225
PdmI GAANNNNTTC 2 cut(s) 1311, 1703
PfeI GAWTC 8 cut(s) 110, 188, 467, 1208, 1604, 1825, 1948, 2232
PflMI CCANNNNNTGG 1 cut(s) 2062
PleI GAGTC 4 cut(s) 127, 249, 523, 1670
PmaCI CACGTG 1 cut(s) 971
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 4 cut(s) 127, 249, 523, 1670
Ppu21I YACGTR 1 cut(s) 971
PpuMI RGGWCCY 1 cut(s) 1480
PshBI ATTAAT 2 cut(s) 1119, 1766
PsiI TTATAA 2 cut(s) 1406, 2211
Psp124BI GAGCTC 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 1480
Psp6I CCWGG 3 cut(s) 1561, 1941, 2247
PspCI CACGTG 1 cut(s) 971
PspGI CCWGG 3 cut(s) 1561, 1941, 2247
PspN4I GGNNCC 3 cut(s) 137, 400, 1054
PspOMI GGGCCC 1 cut(s) 135
PspPI GGNCC 9 cut(s) 24, 87, 105, 135, 136, 219, 582, 1374, 1480
PspPPI RGGWCCY 1 cut(s) 1480
PstI CTGCAG 1 cut(s) 760
PsuI RGATCY 3 cut(s) 1961, 2138, 2197
PteI GCGCGC 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 1832
RsaI GTAC 3 cut(s) 572, 685, 2286
RsaNI GTAC 3 cut(s) 571, 684, 2285
RseI CAYNNNNRTG 3 cut(s) 183, 909, 1538
Rsr2I CGGWCCG 1 cut(s) 87
RsrII CGGWCCG 1 cut(s) 87
SacI GAGCTC 1 cut(s) 695
SaqAI TTAA 8 cut(s) 162, 203, 321, 962, 1119, 1260, 1337, 1766
Sau3AI GATC 9 cut(s) 677, 1010, 1236, 1501, 1961, 2000, 2138, 2197, 2258
Sau96I GGNCC 9 cut(s) 24, 87, 105, 135, 136, 219, 582, 1374, 1480
SchI GAGTC 4 cut(s) 128, 250, 524, 1670
ScrFI CCNGG 8 cut(s) 222, 223, 490, 491, 1545, 1563, 1943, 2249
SduI GDGCHC 6 cut(s) 76, 139, 695, 893, 1545, 2176
SfaNI GCATC 6 cut(s) 629, 1765, 2068, 2073, 2116, 2257
SfcI CTRYAG 3 cut(s) 756, 1107, 1152
SinI GGWCC 3 cut(s) 24, 87, 1480
SmaI CCCGGG 2 cut(s) 223, 491
SmiMI CAYNNNNRTG 3 cut(s) 183, 909, 1538
SmlI CTYRAG 1 cut(s) 1526
SmoI CTYRAG 1 cut(s) 1526
SseBI AGGCCT 1 cut(s) 1790
SspMI CTAG 8 cut(s) 687, 909, 1014, 1442, 1638, 1742, 1970, 2201
SstI GAGCTC 1 cut(s) 695
StuI AGGCCT 1 cut(s) 1790
StyD4I CCNGG 8 cut(s) 220, 221, 488, 489, 1543, 1561, 1941, 2247
StyI CCWWGG 3 cut(s) 54, 1182, 1644
TaaI ACNGT 7 cut(s) 143, 726, 863, 948, 1175, 1279, 1391
TaiI ACGT 1 cut(s) 973
TaqI TCGA 4 cut(s) 509, 676, 1456, 1955
TatI WGTACW 3 cut(s) 570, 683, 2284
TauI GCSGC 3 cut(s) 96, 99, 2273
TfiI GAWTC 8 cut(s) 110, 188, 467, 1208, 1604, 1825, 1948, 2232
Tru1I TTAA 8 cut(s) 162, 203, 321, 962, 1119, 1260, 1337, 1766
Tru9I TTAA 8 cut(s) 162, 203, 321, 962, 1119, 1260, 1337, 1766
TscAI CASTG 3 cut(s) 190, 1246, 1586
TseFI GTSAC 2 cut(s) 863, 1577
TseI GCWGC 7 cut(s) 542, 1218, 1832, 1927, 2129, 2243, 2273
Tsp45I GTSAC 2 cut(s) 863, 1577
TspDTI ATGAA 8 cut(s) 185, 330, 636, 861, 1287, 1522, 1569, 1596
TspGWI ACGGA 3 cut(s) 753, 1011, 1918
TspMI CCCGGG 2 cut(s) 221, 489
TspRI CASTG 3 cut(s) 190, 1246, 1586
Van91I CCANNNNNTGG 1 cut(s) 2062
VneI GTGCAC 1 cut(s) 889
VpaK11BI GGWCC 3 cut(s) 24, 87, 1480
VspI ATTAAT 2 cut(s) 1119, 1766
XagI CCTNNNNNAGG 2 cut(s) 1487, 1721
XapI RAATTY 4 cut(s) 699, 964, 1445, 2147
XbaI TCTAGA 2 cut(s) 1013, 1637
XceI RCATGY 1 cut(s) 784
XcmI CCANNNNNNNNNTGG 2 cut(s) 61, 379
XmaI CCCGGG 2 cut(s) 221, 489
XmiI GTMKAC 2 cut(s) 350, 1801
XmnI GAANNNNTTC 2 cut(s) 1311, 1703
XspI CTAG 8 cut(s) 687, 909, 1014, 1442, 1638, 1742, 1970, 2201
Zsp2I ATGCAT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.