pycom07g05120

Enhancer of polycomb-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008000
Physical Location & Seq
Forward (+)
4390360 .. 4393810
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05120.1

Sequence Viewer

Length: 2463 bp
ATGTTGTGGTTCCGCGTGTTCCCCAACCTTGAGTACTCCCTCCCCGAGTCTGGGGCTGAGTCGACTCGGAGATCGACTAGGCGACTCAGCGAGTCGAGTAGGAGGAGGAAGAGGAGGAGTGCGCCCAGAGTGGTGTTGCTCCGGAATCTGAGCACGGAAATGCCATCGGTGGAGAGGAGGCGGACAACGAGGGTTTTCGGGATGGGGATGGCCAAGGGCGGTGTGGACGGGGCTCGGGTTTTGCGGTCCGGGCGCCGCCTCTGGCCCGAATCGTCCGAGTCCAAGCTCGAGAGGGCCCACCGTGGCGATGAGGACTGGCTCAAAGTCATGAAAAGCCGTGCCAGTCATTCTGTTGTTTCTTTAAAGCCCAAGAGATGGCCCGGGAGCCACCACATCCGGAGTTCGAAGCAAACCCCCGTCGTTTCGAAAGTTCCAGTGGCTAAGAAGCCTGAAAGTAATGACCTTTTGGCAGATGAGCTTAAACAGAAGAAGATGTATGGGATTGTGTACACCCGAAAGAGGAAGCGCACCACTGCGGATGTGGAGAAGGAAGGTGGTTCCAAGGACAGAATGTATGGGCGGAGATTTGTTCGGAGGCAGAGGAGGAAGACGAGCGGGGAGCTGGATCCCCAAGTGGGTGTTGTGTCCCCCGAAGCGCTTTGTGTTTCGTTTGAGTCTTCGTGCAGTCGGAGATACTGGTCTGCTCGTTTTTTGTGTTCGGTTTTTGTGTGCATGACGAGGGCTAGTCTGGGATTGACTGAGCTCTCTGAATTCCTTGCATCAGAGCCAATTGGTAGCTTTTTTTCCTCCTGTGGCATTCAGTTCTCGCGGGTTCGATCTTGTAGTAGGAGCTCTGGAATTTGCAAGTTATTTGGGGCCAAACAGTTTATTCCATTGTTTTCTGTGGACTTTTCTGCAGTTCCTTTTTGTTTTATGGACATGCATATCACCATGCATCTCCGATATAAATGTCAGCTAACTGTAAACAATCAGATTGATGGGCATGAAAGTGATGATGACTATGATGAAGAAGTTGATTGTGTTGAAAGCACACCGGTTTCGCATTCTAGTGCCAGAGTTACAAAATTACCTTGTAAAAACTCACAGTATAGGAATAGTTTAACTTCCCGTGGTACTCAGAAGAGGCGGAGTTCATTGAGGACAAGGCGATCTAGAAATCCTTCGCTGGTTGCCTTACGGAAGCCCAATGGAGCCTTAGTTTCTGAGCTATTCAGTTCTATGAAGAATGGCCACCCTTTTTCTTCTGTAGCCTCTAATAACAAGCGGAGGAAGTCAATTCCAACTTCCCCTGTAGGTAACCTGATAGAAGACGGTTCAACCATGGAGAGATCAGCACGGGATTTAGATTCTACATGTTGCTCGGCAAATATATTGATCACTGAAGCGGGCAAATGTTACAGGGAAGATGGAGCAACTGTCATGTTAGAAACCTCTTCATCTGGAGAGCGGCTTCTTGTGATCAAGAAAGATGGATTAACCCGTTACACCCACAAGGCAGAGAAAGTAATGAGGCCCTTCTCTAAAAACCGTCTTACGAATTTTATAATGTGGTCTGCGGATAATAATGGGGAAAATAGTTGGAAGCTAGAATTTCCCAATCAATCTGATTGGTGTATTTTCAAGGACCTTTACAAGGAGTGCTCTGGTCGTTCTGTCCCATCTCCAGCTGTGAAGTTCATCCCCGTGCCCGGTGTGCATGGAGTCCCAGGATATGCAAATGGTCACCATGCTCCTTTCCACAGGCCCGATTCATACATATCTCTGAATGATGATGAGTTGTCTAGAGCCATGAAAAAGAGAAATGCAAATTATGACATGGACTCTGATGATGAGGAGTGGCTGAAGAAGTTCAACAGTGACTTTTTTGCTGAGGATGAACTTGATGACCATGTTTCCGAGGATAACTTTGAATTAATTGTCGATGCAATCGAGAAGGCATTTTATTATAGACCATATGATTTTTCTGACAACAATCCAGCTGCAAATCTTTGTCCGGAGATGGGAAGGAGGGAAGTAATAGAGGCTGTATATAGGTATTGGATGAATAAACGGAAGCAGAAGCGAGTATCATCACTGCTTAGGGTGTTCCAGGCGTATCAGTCGAAGAGATCTCTGCTAGATCCAAAGCCTATTATCCGCAGGAGACGATCATTCAAGCGACAGCCAAGTCAAGTTGGTAGAGGAAAACAACCAAGTTTCTTGCAAGCTATGGCTGCGGAACAAGATGCGATGCAAGAGCAGAATGCTGTGTATAAAGTTGAAGAAGCGAAAGCTGCGGCCGAGAGGTCTGTGGAATTAGCTATCCGCAAACGACAACGGGCTCAGTTACTCATGCAGAATGCAGATTTGGTAACTTATAAAGCGACGATGGCATTTAGAATCGCTGAAGCAGCCCTAGTTCTCGGATCACCGGATGCCGCTGCTGATTATGTACTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

821

Amino Acids

93.41

Weight (kDa)

9.71

Isoelectric Point (pI)

67.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 561 - 652 1e-11 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1565, 2379
AasI GACNNNNNNGTC 1 cut(s) 2187
AccB1I GGYRCC 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 375
AccBSI CCGCTC 2 cut(s) 615, 1468
AccI GTMKAC 1 cut(s) 62
AccII CGCG 2 cut(s) 15, 829
AccIII TCCGGA 3 cut(s) 141, 396, 2014
AclWI GGATC 4 cut(s) 620, 633, 2135, 2434
AcoI YGGCCR 3 cut(s) 210, 1249, 2298
AcsI RAATTY 4 cut(s) 770, 858, 1558, 1610
AcuI CTGAAG 3 cut(s) 1422, 1883, 2427
AcyI GRCGYC 1 cut(s) 253
AfaI GTAC 4 cut(s) 35, 509, 1135, 2454
AfeI AGCGCT 1 cut(s) 657
AfiI CCNNNNNNNGG 8 cut(s) 50, 51, 375, 519, 635, 1654, 1709, 2021
AflIII ACRYGT 1 cut(s) 1373
AgeI ACCGGT 1 cut(s) 1054
AgsI TTSAA 7 cut(s) 1046, 1338, 1642, 1873, 1931, 2176, 2282
AjnI CCWGG 2 cut(s) 1726, 2109
AjuI GAANNNNNNNTTGG 2 cut(s) 2351, 2383
Alw21I GWGCWC 4 cut(s) 155, 765, 854, 1664
Alw26I GTCTC 1 cut(s) 2158
AlwI GGATC 4 cut(s) 620, 633, 2135, 2434
Ama87I CYCGRG 4 cut(s) 44, 234, 287, 380
Aor13HI TCCGGA 3 cut(s) 141, 396, 2014
Aor51HI AGCGCT 1 cut(s) 657
AoxI GGCC 9 cut(s) 210, 263, 294, 377, 876, 1249, 1532, 1763, 2298
ApaI GGGCCC 1 cut(s) 298
ApeKI GCWGC 5 cut(s) 2000, 2234, 2294, 2411, 2441
ApoI RAATTY 4 cut(s) 770, 858, 1558, 1610
AseI ATTAAT 1 cut(s) 1934
AsiGI ACCGGT 1 cut(s) 1054
Asp700I GAANNNNTTC 2 cut(s) 1180, 1868
AspLEI GCGC 4 cut(s) 124, 255, 528, 658
AspS9I GGNCC 9 cut(s) 246, 264, 294, 295, 378, 876, 1533, 1645, 1764
AsuC2I CCSGG 4 cut(s) 250, 381, 382, 1710
AsuHPI GGTGA 3 cut(s) 940, 1736, 2421
AsuII TTCGAA 2 cut(s) 404, 425
AvaI CYCGRG 4 cut(s) 44, 234, 287, 380
AvaII GGWCC 2 cut(s) 246, 1645
BaeGI GKGCMC 2 cut(s) 298, 1710
BalI TGGCCA 2 cut(s) 212, 1251
BamHI GGATCC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 252
BanII GRGCYC 5 cut(s) 235, 298, 765, 854, 2344
BbsI GAAGAC 3 cut(s) 614, 669, 1335
Bbv12I GWGCWC 4 cut(s) 155, 765, 854, 1664
BbvCI CCTCAGC 1 cut(s) 1890
BbvI GCAGC 5 cut(s) 1987, 2221, 2281, 2423, 2428
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 2 cut(s) 1728, 2111
BclI TGATCA 2 cut(s) 1395, 1479
BcnI CCSGG 4 cut(s) 250, 381, 382, 1710
BcoDI GTCTC 1 cut(s) 2158
BfaI CTAG 8 cut(s) 78, 744, 1068, 1173, 1607, 1803, 2138, 2417
BfmI CTRYAG 3 cut(s) 915, 1266, 1311
BfoI RGCGCY 2 cut(s) 256, 659
BglII AGATCT 1 cut(s) 2129
BisI GCNGC 9 cut(s) 256, 1469, 2001, 2235, 2295, 2298, 2412, 2439, 2442
BlsI GCNGC 9 cut(s) 257, 1470, 2002, 2236, 2296, 2299, 2413, 2440, 2443
BmcAI AGTACT 1 cut(s) 35
Bme1390I CCNGG 6 cut(s) 250, 381, 382, 1710, 1728, 2111
Bme18I GGWCC 2 cut(s) 246, 1645
BmeT110I CYCGRG 4 cut(s) 44, 234, 287, 380
BmgT120I GGNCC 9 cut(s) 246, 264, 294, 295, 378, 876, 1533, 1645, 1764
BmiI GGNNCC 8 cut(s) 11, 254, 296, 386, 559, 627, 877, 1213
BmrFI CCNGG 6 cut(s) 250, 381, 382, 1710, 1728, 2111
BmsI GCATC 6 cut(s) 788, 964, 1933, 2236, 2241, 2425
BpiI GAAGAC 3 cut(s) 614, 669, 1335
BpmI CTGGAG 2 cut(s) 1482, 1668
Bpu10I CCTNAGC 2 cut(s) 1890, 2099
Bpu14I TTCGAA 2 cut(s) 404, 425
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 4 cut(s) 250, 381, 382, 1710
BsaHI GRCGYC 1 cut(s) 253
BsaJI CCNNGG 8 cut(s) 213, 301, 380, 561, 1129, 1341, 1726, 1917
BsaWI WCCGGW 5 cut(s) 141, 396, 1054, 2014, 2431
BsaXI ACNNNNNCTCC 4 cut(s) 1203, 1233, 1422, 1452
Bsc4I CCNNNNNNNGG 8 cut(s) 50, 51, 375, 519, 635, 1654, 1709, 2021
Bse118I RCCGGY 1 cut(s) 1054
Bse1I ACTGG 4 cut(s) 320, 342, 434, 701
BseAI TCCGGA 3 cut(s) 141, 396, 2014
BseBI CCWGG 2 cut(s) 1728, 2111
BseDI CCNNGG 8 cut(s) 213, 301, 380, 561, 1129, 1341, 1726, 1917
BseGI GGATG 8 cut(s) 207, 213, 393, 544, 1698, 1900, 2067, 2440
BseLI CCNNNNNNNGG 8 cut(s) 50, 51, 375, 519, 635, 1654, 1709, 2021
BseMII CTCAG 8 cut(s) 48, 100, 140, 750, 1151, 1215, 1881, 2357
BseNI ACTGG 4 cut(s) 320, 342, 434, 701
BseRI GAGGAG 6 cut(s) 118, 127, 130, 190, 616, 1868
BseSI GKGCMC 2 cut(s) 298, 1710
BseX3I CGGCCG 1 cut(s) 2298
BseXI GCAGC 5 cut(s) 1987, 2221, 2281, 2423, 2428
BsgI GTGCAG 1 cut(s) 703
Bsh1236I CGCG 2 cut(s) 15, 829
Bsh1285I CGRYCG 1 cut(s) 2301
BshFI GGCC 9 cut(s) 212, 265, 296, 379, 878, 1251, 1534, 1765, 2300
BshNI GGYRCC 1 cut(s) 252
BshTI ACCGGT 1 cut(s) 1054
BsiEI CGRYCG 1 cut(s) 2301
BsiHKAI GWGCWC 4 cut(s) 155, 765, 854, 1664
BsiHKCI CYCGRG 4 cut(s) 44, 234, 287, 380
BsiSI CCGG 8 cut(s) 142, 249, 381, 397, 1055, 1710, 2015, 2432
BslFI GGGAC 3 cut(s) 631, 1661, 1709
BslI CCNNNNNNNGG 8 cut(s) 50, 51, 375, 519, 635, 1654, 1709, 2021
BsmAI GTCTC 1 cut(s) 2158
BsmBI CGTCTC 1 cut(s) 2158
BsmFI GGGAC 3 cut(s) 631, 1661, 1709
BsmI GAATGC 4 cut(s) 816, 1063, 2269, 2365
BsnI GGCC 9 cut(s) 212, 265, 296, 379, 878, 1251, 1534, 1765, 2300
BsoBI CYCGRG 4 cut(s) 44, 234, 287, 380
Bsp119I TTCGAA 2 cut(s) 404, 425
Bsp120I GGGCCC 1 cut(s) 294
Bsp1286I GDGCHC 8 cut(s) 155, 235, 298, 765, 854, 1664, 1710, 2344
Bsp13I TCCGGA 3 cut(s) 141, 396, 2014
Bsp1407I TGTACA 1 cut(s) 507
Bsp19I CCATGG 1 cut(s) 1341
BspANI GGCC 9 cut(s) 212, 265, 296, 379, 878, 1251, 1534, 1765, 2300
BspCNI CTCAG 8 cut(s) 49, 99, 141, 751, 1150, 1216, 1882, 2356
BspEI TCCGGA 3 cut(s) 141, 396, 2014
BspFNI CGCG 2 cut(s) 15, 829
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 8 cut(s) 11, 254, 296, 386, 559, 627, 877, 1213
BspMAI CTGCAG 1 cut(s) 919
BspPI GGATC 4 cut(s) 620, 633, 2135, 2434
BspT104I TTCGAA 2 cut(s) 404, 425
BspT107I GGYRCC 1 cut(s) 252
BsrBI CCGCTC 2 cut(s) 615, 1468
BsrFI RCCGGY 1 cut(s) 1054
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 4 cut(s) 320, 342, 434, 701
BssAI RCCGGY 1 cut(s) 1054
BssECI CCNNGG 8 cut(s) 213, 301, 380, 561, 1129, 1341, 1726, 1917
BssNI GRCGYC 1 cut(s) 253
BssT1I CCWWGG 3 cut(s) 213, 561, 1341
Bst2UI CCWGG 2 cut(s) 1728, 2111
Bst4CI ACNGT 8 cut(s) 302, 885, 982, 1107, 1334, 1438, 1550, 1877
Bst6I CTCTTC 4 cut(s) 104, 1136, 1459, 2120
BstACI GRCGYC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 507
BstBI TTCGAA 2 cut(s) 404, 425
BstC8I GCNNGC 2 cut(s) 1408, 2226
BstDSI CCRYGG 3 cut(s) 301, 1129, 1341
BstEII GGTNACC 2 cut(s) 1316, 1742
BstENI CCTNNNNNAGG 1 cut(s) 1652
BstF5I GGATG 8 cut(s) 207, 213, 393, 544, 1698, 1900, 2067, 2440
BstFNI CGCG 2 cut(s) 15, 829
BstH2I RGCGCY 2 cut(s) 256, 659
BstHHI GCGC 4 cut(s) 124, 255, 528, 658
BstMAI GTCTC 1 cut(s) 2158
BstMCI CGRYCG 1 cut(s) 2301
BstMWI GCNNNNNNNGC 6 cut(s) 250, 1714, 2234, 2294, 2390, 2411
BstNI CCWGG 2 cut(s) 1728, 2111
BstNSI RCATGY 2 cut(s) 943, 1377
BstPI GGTNACC 2 cut(s) 1316, 1742
BstSCI CCNGG 6 cut(s) 248, 379, 380, 1708, 1726, 2109
BstSFI CTRYAG 3 cut(s) 915, 1266, 1311
BstSLI GKGCMC 2 cut(s) 298, 1710
BstUI CGCG 2 cut(s) 15, 829
BstV1I GCAGC 5 cut(s) 1987, 2221, 2281, 2423, 2428
BstV2I GAAGAC 3 cut(s) 614, 669, 1335
BstX2I RGATCY 3 cut(s) 625, 2129, 2140
BstYI RGATCY 3 cut(s) 625, 2129, 2140
BstZI CGGCCG 1 cut(s) 2298
BsuRI GGCC 9 cut(s) 212, 265, 296, 379, 878, 1251, 1534, 1765, 2300
BtgI CCRYGG 3 cut(s) 301, 1129, 1341
BtgZI GCGATG 2 cut(s) 321, 2264
BtsCI GGATG 8 cut(s) 207, 213, 393, 544, 1698, 1900, 2067, 2440
BtsI GCAGTG 2 cut(s) 531, 2093
BtsIMutI CAGTG 5 cut(s) 441, 531, 1398, 1882, 2093
Cac8I GCNNGC 2 cut(s) 1408, 2226
CciI TCATGA 1 cut(s) 327
CfoI GCGC 4 cut(s) 124, 255, 528, 658
Cfr10I RCCGGY 1 cut(s) 1054
Cfr13I GGNCC 9 cut(s) 246, 264, 294, 295, 378, 876, 1533, 1645, 1764
Cfr9I CCCGGG 1 cut(s) 380
CpoI CGGWCCG 1 cut(s) 246
Csp6I GTAC 4 cut(s) 34, 508, 1134, 2453
CspAI ACCGGT 1 cut(s) 1054
CspI CGGWCCG 1 cut(s) 246
CviQI GTAC 4 cut(s) 34, 508, 1134, 2453
DinI GGCGCC 1 cut(s) 254
DraI TTTAAA 1 cut(s) 363
DrdI GACNNNNNNGTC 1 cut(s) 2187
DseDI GACNNNNNNGTC 1 cut(s) 2187
EaeI YGGCCR 3 cut(s) 210, 1249, 2298
EagI CGGCCG 1 cut(s) 2298
Eam1104I CTCTTC 4 cut(s) 104, 1136, 1459, 2120
EarI CTCTTC 4 cut(s) 104, 1136, 1459, 2120
EciI GGCGGA 3 cut(s) 196, 595, 1162
Ecl136II GAGCTC 2 cut(s) 763, 852
EclXI CGGCCG 1 cut(s) 2298
Eco130I CCWWGG 3 cut(s) 213, 561, 1341
Eco24I GRGCYC 5 cut(s) 235, 298, 765, 854, 2344
Eco47I GGWCC 2 cut(s) 246, 1645
Eco47III AGCGCT 1 cut(s) 657
Eco52I CGGCCG 1 cut(s) 2298
Eco53kI GAGCTC 2 cut(s) 763, 852
Eco57I CTGAAG 3 cut(s) 1422, 1883, 2427
Eco88I CYCGRG 4 cut(s) 44, 234, 287, 380
Eco91I GGTNACC 2 cut(s) 1316, 1742
EcoICRI GAGCTC 2 cut(s) 763, 852
EcoNI CCTNNNNNAGG 1 cut(s) 1652
EcoO109I RGGNCCY 3 cut(s) 294, 1533, 1645
EcoO65I GGTNACC 2 cut(s) 1316, 1742
EcoRI GAATTC 1 cut(s) 770
EcoRII CCWGG 2 cut(s) 1726, 2109
EcoT14I CCWWGG 3 cut(s) 213, 561, 1341
EcoT22I ATGCAT 2 cut(s) 945, 957
EcoT38I GRGCYC 5 cut(s) 235, 298, 765, 854, 2344
EgeI GGCGCC 1 cut(s) 254
EheI GGCGCC 1 cut(s) 254
ErhI CCWWGG 3 cut(s) 213, 561, 1341
Esp3I CGTCTC 1 cut(s) 2158
FaqI GGGAC 3 cut(s) 631, 1661, 1709
FauI CCCGC 3 cut(s) 608, 822, 1399
FauNDI CATATG 1 cut(s) 1975
FbaI TGATCA 2 cut(s) 1395, 1479
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 9 cut(s) 256, 1469, 2001, 2235, 2295, 2298, 2412, 2439, 2442
FokI GGATG 8 cut(s) 214, 220, 380, 551, 1685, 1907, 2074, 2447
FriOI GRGCYC 5 cut(s) 235, 298, 765, 854, 2344
Fsp4HI GCNGC 9 cut(s) 256, 1469, 2001, 2235, 2295, 2298, 2412, 2439, 2442
FspBI CTAG 8 cut(s) 78, 744, 1068, 1173, 1607, 1803, 2138, 2417
GlaI GCGC 4 cut(s) 123, 254, 527, 657
GluI GCNGC 9 cut(s) 256, 1469, 2001, 2235, 2295, 2298, 2412, 2439, 2442
GsuI CTGGAG 2 cut(s) 1482, 1668
HaeII RGCGCY 2 cut(s) 256, 659
HaeIII GGCC 9 cut(s) 212, 265, 296, 379, 878, 1251, 1534, 1765, 2300
HapII CCGG 8 cut(s) 142, 249, 381, 397, 1055, 1710, 2015, 2432
HhaI GCGC 4 cut(s) 124, 255, 528, 658
Hin1I GRCGYC 1 cut(s) 253
Hin6I GCGC 4 cut(s) 122, 253, 526, 656
HinP1I GCGC 4 cut(s) 122, 253, 526, 656
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HpaII CCGG 8 cut(s) 142, 249, 381, 397, 1055, 1710, 2015, 2432
HphI GGTGA 3 cut(s) 940, 1736, 2421
Hpy166II GTNNAC 6 cut(s) 63, 226, 508, 510, 907, 985
Hpy8I GTNNAC 6 cut(s) 63, 226, 508, 510, 907, 985
Hpy99I CGWCG 2 cut(s) 422, 2389
HpyAV CCTTC 6 cut(s) 541, 545, 1191, 1546, 1948, 2019
HpyCH4III ACNGT 8 cut(s) 302, 885, 982, 1107, 1334, 1438, 1550, 1877
HpyF10VI GCNNNNNNNGC 6 cut(s) 250, 1714, 2234, 2294, 2390, 2411
Hsp92I GRCGYC 1 cut(s) 253
HspAI GCGC 4 cut(s) 122, 253, 526, 656
KasI GGCGCC 1 cut(s) 252
Kpn2I TCCGGA 3 cut(s) 141, 396, 2014
Ksp22I TGATCA 2 cut(s) 1395, 1479
LmnI GCTCC 7 cut(s) 144, 384, 619, 849, 1211, 1430, 1756
Lsp1109I GCAGC 5 cut(s) 1987, 2221, 2281, 2423, 2428
LweI GCATC 6 cut(s) 788, 964, 1933, 2236, 2241, 2425
MaeI CTAG 8 cut(s) 78, 744, 1068, 1173, 1607, 1803, 2138, 2417
MaeIII GTNAC 8 cut(s) 1078, 1316, 1415, 1502, 1742, 1877, 2346, 2371
MbiI CCGCTC 2 cut(s) 615, 1468
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 3 cut(s) 625, 2129, 2140
MhlI GDGCHC 8 cut(s) 155, 235, 298, 765, 854, 1664, 1710, 2344
MlsI TGGCCA 2 cut(s) 212, 1251
MluNI TGGCCA 2 cut(s) 212, 1251
Mly113I GGCGCC 1 cut(s) 253
MlyI GAGTC 9 cut(s) 56, 58, 68, 78, 101, 287, 683, 1731, 1835
MmeI TCCRAC 3 cut(s) 668, 1325, 1580
Mox20I TGGCCA 2 cut(s) 212, 1251
Mph1103I ATGCAT 2 cut(s) 945, 957
MroI TCCGGA 3 cut(s) 141, 396, 2014
MroXI GAANNNNTTC 2 cut(s) 1180, 1868
MscI TGGCCA 2 cut(s) 212, 1251
MseI TTAA 5 cut(s) 362, 480, 1121, 1496, 1934
MslI CAYNNNNRTG 4 cut(s) 158, 1008, 1068, 1703
Msp20I TGGCCA 2 cut(s) 212, 1251
MspA1I CMGCKG 3 cut(s) 1688, 2000, 2441
MspI CCGG 8 cut(s) 142, 249, 381, 397, 1055, 1710, 2015, 2432
MspR9I CCNGG 6 cut(s) 250, 381, 382, 1710, 1728, 2111
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 4 cut(s) 816, 1063, 2269, 2365
MvaI CCWGG 2 cut(s) 1728, 2111
MvnI CGCG 2 cut(s) 15, 829
MwoI GCNNNNNNNGC 6 cut(s) 250, 1714, 2234, 2294, 2390, 2411
NarI GGCGCC 1 cut(s) 253
NciI CCSGG 4 cut(s) 250, 381, 382, 1710
NcoI CCATGG 1 cut(s) 1341
NdeI CATATG 1 cut(s) 1975
NlaIV GGNNCC 8 cut(s) 11, 254, 296, 386, 559, 627, 877, 1213
NmeAIII GCCGAG 2 cut(s) 1361, 2326
NmuCI GTSAC 2 cut(s) 1742, 1877
NsiI ATGCAT 2 cut(s) 945, 957
NspI RCATGY 2 cut(s) 943, 1377
NspV TTCGAA 2 cut(s) 404, 425
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 327
PciI ACATGT 1 cut(s) 1373
PcsI WCGNNNNNNNCGW 1 cut(s) 1947
PctI GAATGC 4 cut(s) 816, 1063, 2269, 2365
PdmI GAANNNNTTC 2 cut(s) 1180, 1868
PfeI GAWTC 5 cut(s) 145, 269, 1367, 1769, 2400
PflMI CCANNNNNTGG 1 cut(s) 375
PinAI ACCGGT 1 cut(s) 1054
PkrI GCNGC 9 cut(s) 257, 1470, 2002, 2236, 2296, 2299, 2413, 2440, 2443
PleI GAGTC 9 cut(s) 55, 58, 67, 78, 100, 286, 682, 1730, 1835
PluTI GGCGCC 1 cut(s) 256
PpsI GAGTC 9 cut(s) 55, 58, 67, 78, 100, 286, 682, 1730, 1835
PpuMI RGGWCCY 1 cut(s) 1645
PscI ACATGT 1 cut(s) 1373
PshBI ATTAAT 1 cut(s) 1934
PsiI TTATAA 2 cut(s) 1565, 2379
Psp124BI GAGCTC 2 cut(s) 765, 854
Psp5II RGGWCCY 1 cut(s) 1645
Psp6I CCWGG 2 cut(s) 1726, 2109
PspEI GGTNACC 2 cut(s) 1316, 1742
PspGI CCWGG 2 cut(s) 1726, 2109
PspN4I GGNNCC 8 cut(s) 11, 254, 296, 386, 559, 627, 877, 1213
PspOMI GGGCCC 1 cut(s) 294
PspPI GGNCC 9 cut(s) 246, 264, 294, 295, 378, 876, 1533, 1645, 1764
PspPPI RGGWCCY 1 cut(s) 1645
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 3 cut(s) 625, 2129, 2140
PvuII CAGCTG 2 cut(s) 1688, 2000
RsaI GTAC 4 cut(s) 35, 509, 1135, 2454
RsaNI GTAC 4 cut(s) 34, 508, 1134, 2453
RseI CAYNNNNRTG 4 cut(s) 158, 1008, 1068, 1703
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SacI GAGCTC 2 cut(s) 765, 854
SalI GTCGAC 1 cut(s) 61
SaqAI TTAA 5 cut(s) 362, 480, 1121, 1496, 1934
SatI GCNGC 9 cut(s) 256, 1469, 2001, 2235, 2295, 2298, 2412, 2439, 2442
Sau96I GGNCC 9 cut(s) 246, 264, 294, 295, 378, 876, 1533, 1645, 1764
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 9 cut(s) 56, 58, 68, 78, 101, 287, 683, 1731, 1835
ScrFI CCNGG 6 cut(s) 250, 381, 382, 1710, 1728, 2111
SduI GDGCHC 8 cut(s) 155, 235, 298, 765, 854, 1664, 1710, 2344
SfaNI GCATC 6 cut(s) 788, 964, 1933, 2236, 2241, 2425
SfcI CTRYAG 3 cut(s) 915, 1266, 1311
SfoI GGCGCC 1 cut(s) 254
Sfr274I CTCGAG 1 cut(s) 287
SfuI TTCGAA 2 cut(s) 404, 425
SinI GGWCC 2 cut(s) 246, 1645
SlaI CTCGAG 1 cut(s) 287
SmaI CCCGGG 1 cut(s) 382
SmiMI CAYNNNNRTG 4 cut(s) 158, 1008, 1068, 1703
SmlI CTYRAG 2 cut(s) 29, 287
SmoI CTYRAG 2 cut(s) 29, 287
SspDI GGCGCC 1 cut(s) 252
SspMI CTAG 8 cut(s) 78, 744, 1068, 1173, 1607, 1803, 2138, 2417
SstI GAGCTC 2 cut(s) 765, 854
StyD4I CCNGG 6 cut(s) 248, 379, 380, 1708, 1726, 2109
StyI CCWWGG 3 cut(s) 213, 561, 1341
TaaI ACNGT 8 cut(s) 302, 885, 982, 1107, 1334, 1438, 1550, 1877
TatI WGTACW 3 cut(s) 33, 507, 2452
TauI GCSGC 4 cut(s) 258, 1471, 2300, 2441
TfiI GAWTC 5 cut(s) 145, 269, 1367, 1769, 2400
Tru1I TTAA 5 cut(s) 362, 480, 1121, 1496, 1934
Tru9I TTAA 5 cut(s) 362, 480, 1121, 1496, 1934
TscAI CASTG 5 cut(s) 441, 538, 1405, 1882, 2100
TseFI GTSAC 2 cut(s) 1742, 1877
TseI GCWGC 5 cut(s) 2000, 2234, 2294, 2411, 2441
Tsp45I GTSAC 2 cut(s) 1742, 1877
TspGWI ACGGA 3 cut(s) 170, 1213, 2086
TspMI CCCGGG 1 cut(s) 380
TspRI CASTG 5 cut(s) 441, 538, 1405, 1882, 2100
Van91I CCANNNNNTGG 1 cut(s) 375
VpaK11BI GGWCC 2 cut(s) 246, 1645
VspI ATTAAT 1 cut(s) 1934
XagI CCTNNNNNAGG 1 cut(s) 1652
XapI RAATTY 4 cut(s) 770, 858, 1558, 1610
XbaI TCTAGA 2 cut(s) 1172, 1802
XceI RCATGY 2 cut(s) 943, 1377
XcmI CCANNNNNNNNNTGG 2 cut(s) 220, 538
XhoI CTCGAG 1 cut(s) 287
XmaI CCCGGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 62
XmnI GAANNNNTTC 2 cut(s) 1180, 1868
XspI CTAG 8 cut(s) 78, 744, 1068, 1173, 1607, 1803, 2138, 2417
ZrmI AGTACT 1 cut(s) 35
Zsp2I ATGCAT 2 cut(s) 945, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.