Rw1G010370

Enhancer of polycomb-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008000
Physical Location & Seq
Reverse (-)
23131334 .. 23135980
4647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010370.1

Sequence Viewer

Length: 2526 bp
ATGAGGCTGGCGTCGCCGTTTTCTGAGATTGAGAGTTTCGGGTCCGAGTTTGGGACTGAGTCGACTCGGAGATCGACTCGGAGACGACGGGCTGCGAGGGAGTCGAGGAGGAGGCCGAGAGTGGTGGTGCTTCGGAATTTGAGGAAGGAAATGCCATCGGTGGAGATGAGGAGGACCACGAGGGTTTTCGGGATGGGGAAGGGGGTGGACGGAGCCCGAGTCTTGCGTTCGGGCCGCCGCCTCTGGCCCGAGTCCTCTGTGGTCAAGCATGAGAGAACCAACGAGGACGAGGACTGGTTCAAGCTCATCAAGAACAATGGGGAAGGTCGTGATTCTGACGCAATTGGTTTAAAGCATAAGGGATGGACCAGAGCCACCCGGCTAGCGGGTCATAAGCCTAAGCCGAATATAGTTGTTTCGGTCAGTCGAGTCCTTGAGAAGCCTGTTGAGACTAAGGTTTTGGCTGATGGGCTCAAGCAGTATAAGAGGTTCGGGATTGTATATACCAGGAAGAGGAAACGGACCGGTCACTCAGTCAATGTGGAGAATCAAAGTGGCTCTGAGGATAGAATGTACGGGCAGAGGTTTGCTAGGAAGAGGAGGAAGACAAGTGGCAGCGGCAGCTTGGTAGGAGCTGAGAGGTTGGATCTTGCAAGCCCTGAAGTGCTTCACATTACATTTGAGTCTTCATGGGGCAGACGAAACTGGGCTGCTCGGCTTTTGTATTCGATTTTGGTGTACATGTCGAGGTCCAGCCTGCAGTTGACTGAGCTTTCTCAATTCCTTGTCTCGGAGCCCCTCAGCATTGTTTTTGCCTTGCCTGGCATTTATTTTTCCTGGGTACGTTCTTCCACTATTGGCTCTGGAGTTTGCAAGTTCTTTGGGAGCAAGGAGTTCATGCCAATGTTTTCTGTGGACTTTTCTGCAGTTCCTCAATGTTTCACGCTCATGCACTCCGAAATGCATTTTAGATACATTTTTCGGCCAATTCTTCCTGTAAATTATCTCAATGATGGGCATGTAGATGATGATGATGATGATGATGTTAATCAGTGTGGCAATGACAGTAATGAGTGTGTAGTCTTGCATGAGGTTGATCACGCCGAAAACAGACCGTTGCATCCGGTTGTAAAAGTTCCCAAATTAGGTTGTAGAAGCACAGTGTATAGGAATGGTTTAAGTTCACGAGGTGGTATTCAAAAGAGGAGGAGTTCATTGAGGAGGAGGAGATCTAGAAATCCTTCTTTTGTTGCTTTACGGAAACCAAATGGAGCCTTGGTTTCAGAATTAAAGAAGAATGGCCTTTCCTTTTCTTCTGTAGCCTTGAATCAGAAGCTTAGGAGGTCAGATTCAACTTGCTCTGCAACAAACTCGAAAGCAGTGAGTTCGAATGCGGATAGATTGGTGCAAGATTTAGATTCGACATGCTGCACTGCAAACTTATTGGTCTCTGAATCAGACAGATGTTATAGGGAAGTTGGAGTAACCATTATGCTAGAAACCTCTTCTGCTGGAGAGTGGCTTCTTGTGGTCAAGAAAGATGGATTAACGCGACTTACTCACAAAGCAGAGAAAGAGAAAGTAATAAGGCCGGGCTCTAACCGTTTTACACATGATATAATCTGGTCCCCAGATAGTGATATTTTGAGGCTAGAGTTTCCGGATCGAAGTGAGTGGATTACTTTCAAGGAGCTTTACAGGGAATGTTCTGATCGTAATGCCACCATTGAGTTCATTCCTGAGCCTGCTTCTGACCGTAATGCCACTGCAAAATCCATTCCTGTGCCAGGGGTGCGTGAAGTCCCAGGTTATGCAGATAGCCACAGTTCTCCGTTTCTCATACCAGAGTCATACATATCTGTGAAGGAAGATGAGTTGTCTAGATCCTTTGCAAAGAAGACGGCGAATTATGACATGGACTCTGAAGATGAGGAATGGCTGAAGAAGTCTAACGTGGACTTTGAAGATCATGAACATGTTTCTGAGGATAAATTTGAATTAATGGTTGATGCCTTTGAGAAGGCATTTTACAGTAAACCATATGATTTCTCTGATGGAATTGCTGCTGCTAGTCATTTTCTGGACATGGGGAAGAGGGATGTAGTAGAAGCTGTATATAGTTATTGGATGAATAAAAGGAAGCAGAAAAAGTCGTCATTGCTTAGGGTCTTCCAGGGGCATCAAGTGAAAAGACCACTGCTGGACTCCAAGCCTGTTATGCGCAAGAGAAGATCATTCAAACGACAGCCTAGTCAATTTGGCAGAGGAAAACAACCTAGTTTCTTGCAAGCAATGGCTGCAGAACAAGATGCTTTGCAAGAACAGAATGCTGTGCTTAAAGTTGAAGAGGCGAAAGCAGCAGCAGACAGATCTGTGGATTTAGCTATCCGTAAACGACAGAGAGCTCAGGTACTTATGCAGAATGCAGATTTGGCAACTTACAAAGCTGCAATGGCATTTAGAATTGCTGAAGCTGCCCAAGTTCTTGGATCACCCGATGCTGCTGCAGTTTATGTACTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

841

Amino Acids

95.52

Weight (kDa)

9.63

Isoelectric Point (pI)

59.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 570 - 674 1.9e-13 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2250
Acc16I TGCGCA 1 cut(s) 2222
AccI GTMKAC 1 cut(s) 62
AccII CGCG 1 cut(s) 1552
AccIII TCCGGA 1 cut(s) 1660
AciI CCGC 5 cut(s) 235, 238, 386, 618, 1394
AclWI GGATC 4 cut(s) 654, 1671, 1878, 2497
AcoI YGGCCR 1 cut(s) 983
AcsI RAATTY 2 cut(s) 136, 1991
AcuI CTGAAG 4 cut(s) 681, 1944, 1961, 2490
AcyI GRCGYC 1 cut(s) 11
AdeI CACNNNGTG 1 cut(s) 1190
AfaI GTAC 5 cut(s) 575, 740, 843, 2412, 2517
AfiI CCNNNNNNNGG 6 cut(s) 51, 385, 513, 790, 1145, 1787
AflIII ACRYGT 2 cut(s) 741, 1975
AgeI ACCGGT 1 cut(s) 524
AgsI TTSAA 9 cut(s) 301, 1199, 1327, 1353, 1687, 1964, 1997, 2239, 2345
AjnI CCWGG 6 cut(s) 506, 820, 836, 1786, 1804, 2172
AjuI GAANNNNNNNTTGG 2 cut(s) 2414, 2446
Alw21I GWGCWC 1 cut(s) 2407
Alw26I GTCTC 4 cut(s) 76, 443, 793, 1453
AlwI GGATC 4 cut(s) 654, 1671, 1878, 2497
Ama87I CYCGRG 2 cut(s) 216, 248
Aor13HI TCCGGA 1 cut(s) 1660
AoxI GGCC 6 cut(s) 113, 232, 245, 983, 1300, 1589
ApoI RAATTY 2 cut(s) 136, 1991
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AseI ATTAAT 1 cut(s) 2000
AsiGI ACCGGT 1 cut(s) 524
Asp700I GAANNNNTTC 2 cut(s) 666, 1240
AspLEI GCGC 1 cut(s) 2223
AspS9I GGNCC 8 cut(s) 42, 174, 232, 246, 366, 522, 750, 1626
AsuC2I CCSGG 2 cut(s) 379, 1593
AsuHPI GGTGA 1 cut(s) 2484
AsuII TTCGAA 1 cut(s) 1388
AsuNHI GCTAGC 1 cut(s) 382
AvaI CYCGRG 2 cut(s) 216, 248
AvaII GGWCC 6 cut(s) 42, 174, 366, 522, 750, 1626
BanII GRGCYC 5 cut(s) 217, 474, 798, 1598, 2407
BarI GAAGNNNNNNTAC 2 cut(s) 2012, 2044
BauI CACGAG 2 cut(s) 178, 1185
BbsI GAAGAC 4 cut(s) 611, 678, 1904, 2161
Bbv12I GWGCWC 1 cut(s) 2407
BbvCI CCTCAGC 1 cut(s) 800
BccI CCATC 7 cut(s) 163, 187, 357, 461, 1007, 1535, 2048
BceAI ACGGC 1 cut(s) 1917
BcgI CGANNNNNNTGC 6 cut(s) 1353, 1368, 1387, 1402, 2486, 2520
BciT130I CCWGG 6 cut(s) 508, 822, 838, 1788, 1806, 2174
BclI TGATCA 1 cut(s) 1096
BcnI CCSGG 2 cut(s) 379, 1593
BcoDI GTCTC 4 cut(s) 76, 443, 793, 1453
BfaI CTAG 9 cut(s) 383, 591, 1233, 1496, 1652, 1881, 2070, 2250, 2277
BfmI CTRYAG 5 cut(s) 758, 924, 1317, 2298, 2505
BglII AGATCT 2 cut(s) 1229, 2369
Bme1390I CCNGG 8 cut(s) 379, 508, 822, 838, 1593, 1788, 1806, 2174
Bme18I GGWCC 6 cut(s) 42, 174, 366, 522, 750, 1626
BmeT110I CYCGRG 2 cut(s) 216, 248
BmgT120I GGNCC 8 cut(s) 42, 174, 232, 246, 366, 522, 750, 1626
BmiI GGNNCC 5 cut(s) 43, 214, 795, 1273, 1628
BmrFI CCNGG 8 cut(s) 379, 508, 822, 838, 1593, 1788, 1806, 2174
BmrI ACTGGG 1 cut(s) 715
BmsI GCATC 5 cut(s) 1129, 1999, 2188, 2299, 2488
BmtI GCTAGC 1 cut(s) 386
BmuI ACTGGG 1 cut(s) 715
BpiI GAAGAC 4 cut(s) 611, 678, 1904, 2161
BplI GAGNNNNNCTC 2 cut(s) 61, 93
BpmI CTGGAG 2 cut(s) 885, 1533
Bpu10I CCTNAGC 6 cut(s) 399, 800, 1337, 1740, 2162, 2406
Bpu14I TTCGAA 1 cut(s) 1388
BpuEI CTTGAG 2 cut(s) 455, 458
BpuMI CCSGG 2 cut(s) 379, 1593
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 1453
BsaJI CCNNGG 5 cut(s) 837, 1275, 1787, 1804, 2173
BsaWI WCCGGW 3 cut(s) 524, 1123, 1660
BsaXI ACNNNNNCTCC 4 cut(s) 204, 234, 1263, 1293
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 385, 513, 790, 1145, 1787
Bse118I RCCGGY 1 cut(s) 524
Bse1I ACTGG 2 cut(s) 299, 710
Bse3DI GCAATG 4 cut(s) 1066, 2156, 2298, 2457
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 1660
BseBI CCWGG 6 cut(s) 508, 822, 838, 1788, 1806, 2174
BseDI CCNNGG 5 cut(s) 837, 1275, 1787, 1804, 2173
BseGI GGATG 5 cut(s) 198, 368, 1120, 2104, 2133
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 6 cut(s) 51, 385, 513, 790, 1145, 1787
BseMI GCAATG 4 cut(s) 1066, 2156, 2298, 2457
BseNI ACTGG 2 cut(s) 299, 710
BseRI GAGGAG 9 cut(s) 121, 124, 184, 613, 1219, 1222, 1234, 1237, 1240
BsgI GTGCAG 1 cut(s) 1414
Bsh1236I CGCG 1 cut(s) 1552
BshFI GGCC 6 cut(s) 115, 234, 247, 985, 1302, 1591
BshTI ACCGGT 1 cut(s) 524
BsiHKAI GWGCWC 1 cut(s) 2407
BsiHKCI CYCGRG 2 cut(s) 216, 248
BsiSI CCGG 5 cut(s) 379, 525, 1124, 1592, 1661
BslFI GGGAC 3 cut(s) 67, 1612, 1787
BslI CCNNNNNNNGG 6 cut(s) 51, 385, 513, 790, 1145, 1787
BsmAI GTCTC 4 cut(s) 76, 443, 793, 1453
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 3 cut(s) 67, 1612, 1787
BsmI GAATGC 3 cut(s) 1396, 2332, 2428
BsnI GGCC 6 cut(s) 115, 234, 247, 985, 1302, 1591
Bso31I GGTCTC 1 cut(s) 1453
BsoBI CYCGRG 2 cut(s) 216, 248
Bsp119I TTCGAA 1 cut(s) 1388
Bsp1286I GDGCHC 5 cut(s) 217, 474, 798, 1598, 2407
Bsp13I TCCGGA 1 cut(s) 1660
Bsp1407I TGTACA 1 cut(s) 738
BspACI CCGC 5 cut(s) 235, 238, 386, 618, 1394
BspANI GGCC 6 cut(s) 115, 234, 247, 985, 1302, 1591
BspEI TCCGGA 1 cut(s) 1660
BspFNI CGCG 1 cut(s) 1552
BspHI TCATGA 1 cut(s) 1969
BspLI GGNNCC 5 cut(s) 43, 214, 795, 1273, 1628
BspMAI CTGCAG 4 cut(s) 762, 928, 2302, 2509
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 4 cut(s) 654, 1671, 1878, 2497
BspT104I TTCGAA 1 cut(s) 1388
BspTNI GGTCTC 1 cut(s) 1453
BsrDI GCAATG 4 cut(s) 1066, 2156, 2298, 2457
BsrFI RCCGGY 1 cut(s) 524
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 2 cut(s) 299, 710
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 5 cut(s) 837, 1275, 1787, 1804, 2173
BssNI GRCGYC 1 cut(s) 11
BssSI CACGAG 2 cut(s) 178, 1185
BssT1I CCWWGG 1 cut(s) 1275
Bst2BI CACGAG 2 cut(s) 178, 1185
Bst2UI CCWGG 6 cut(s) 508, 822, 838, 1788, 1806, 2174
Bst4CI ACNGT 7 cut(s) 1067, 1116, 1162, 1604, 1757, 1826, 2033
Bst6I CTCTTC 5 cut(s) 506, 590, 1510, 2087, 2340
BstACI GRCGYC 1 cut(s) 11
BstAPI GCANNNNNTGC 1 cut(s) 2297
BstAUI TGTACA 1 cut(s) 738
BstBI TTCGAA 1 cut(s) 1388
BstC8I GCNNGC 6 cut(s) 9, 384, 655, 758, 1746, 2289
BstENI CCTNNNNNAGG 1 cut(s) 1785
BstF5I GGATG 5 cut(s) 198, 368, 1120, 2104, 2133
BstFNI CGCG 1 cut(s) 1552
BstHHI GCGC 1 cut(s) 2223
BstMAI GTCTC 4 cut(s) 76, 443, 793, 1453
BstMWI GCNNNNNNNGC 9 cut(s) 13, 621, 1792, 2218, 2297, 2357, 2432, 2453, 2474
BstNI CCWGG 6 cut(s) 508, 822, 838, 1788, 1806, 2174
BstNSI RCATGY 4 cut(s) 745, 1022, 1428, 1979
BstSCI CCNGG 8 cut(s) 377, 506, 820, 836, 1591, 1786, 1804, 2172
BstSFI CTRYAG 5 cut(s) 758, 924, 1317, 2298, 2505
BstUI CGCG 1 cut(s) 1552
BstV2I GAAGAC 4 cut(s) 611, 678, 1904, 2161
BstX2I RGATCY 4 cut(s) 646, 1229, 1883, 2369
BstXI CCANNNNNNTGG 1 cut(s) 2486
BstYI RGATCY 4 cut(s) 646, 1229, 1883, 2369
BsuRI GGCC 6 cut(s) 115, 234, 247, 985, 1302, 1591
BtsCI GGATG 5 cut(s) 198, 368, 1120, 2104, 2133
BtsI GCAGTG 4 cut(s) 1386, 1431, 1764, 2195
BtsIMutI CAGTG 6 cut(s) 1058, 1167, 1386, 1431, 1764, 2195
Cac8I GCNNGC 6 cut(s) 9, 384, 655, 758, 1746, 2289
CciI TCATGA 1 cut(s) 1969
CfoI GCGC 1 cut(s) 2223
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 8 cut(s) 42, 174, 232, 246, 366, 522, 750, 1626
CpoI CGGWCCG 1 cut(s) 522
CseI GACGC 1 cut(s) 347
Csp6I GTAC 5 cut(s) 574, 739, 842, 2411, 2516
CspAI ACCGGT 1 cut(s) 524
CspI CGGWCCG 1 cut(s) 522
CviQI GTAC 5 cut(s) 574, 739, 842, 2411, 2516
DraI TTTAAA 1 cut(s) 351
DraIII CACNNNGTG 1 cut(s) 1190
DrdI GACNNNNNNGTC 1 cut(s) 2250
DseDI GACNNNNNNGTC 1 cut(s) 2250
EaeI YGGCCR 1 cut(s) 983
Eam1104I CTCTTC 5 cut(s) 506, 590, 1510, 2087, 2340
EarI CTCTTC 5 cut(s) 506, 590, 1510, 2087, 2340
Ecl136II GAGCTC 1 cut(s) 2405
Eco130I CCWWGG 1 cut(s) 1275
Eco24I GRGCYC 5 cut(s) 217, 474, 798, 1598, 2407
Eco31I GGTCTC 1 cut(s) 1453
Eco47I GGWCC 6 cut(s) 42, 174, 366, 522, 750, 1626
Eco53kI GAGCTC 1 cut(s) 2405
Eco57I CTGAAG 4 cut(s) 681, 1944, 1961, 2490
Eco88I CYCGRG 2 cut(s) 216, 248
EcoICRI GAGCTC 1 cut(s) 2405
EcoNI CCTNNNNNAGG 1 cut(s) 1785
EcoRII CCWGG 6 cut(s) 506, 820, 836, 1786, 1804, 2172
EcoT14I CCWWGG 1 cut(s) 1275
EcoT22I ATGCAT 1 cut(s) 966
EcoT38I GRGCYC 5 cut(s) 217, 474, 798, 1598, 2407
ErhI CCWWGG 1 cut(s) 1275
Esp3I CGTCTC 1 cut(s) 76
FaqI GGGAC 3 cut(s) 67, 1612, 1787
FauI CCCGC 1 cut(s) 379
FauNDI CATATG 1 cut(s) 2041
FbaI TGATCA 1 cut(s) 1096
FblI GTMKAC 1 cut(s) 62
FokI GGATG 5 cut(s) 205, 375, 1107, 2111, 2140
FriOI GRGCYC 5 cut(s) 217, 474, 798, 1598, 2407
FspBI CTAG 9 cut(s) 383, 591, 1233, 1496, 1652, 1881, 2070, 2250, 2277
FspI TGCGCA 1 cut(s) 2222
GlaI GCGC 1 cut(s) 2222
GsuI CTGGAG 2 cut(s) 885, 1533
HaeIII GGCC 6 cut(s) 115, 234, 247, 985, 1302, 1591
HapII CCGG 5 cut(s) 379, 525, 1124, 1592, 1661
HgaI GACGC 1 cut(s) 347
HhaI GCGC 1 cut(s) 2223
Hin1I GRCGYC 1 cut(s) 11
Hin6I GCGC 1 cut(s) 2221
HinP1I GCGC 1 cut(s) 2221
HincII GTYRAC 2 cut(s) 63, 765
HindII GTYRAC 2 cut(s) 63, 765
HindIII AAGCTT 1 cut(s) 1334
HpaII CCGG 5 cut(s) 379, 525, 1124, 1592, 1661
HphI GGTGA 1 cut(s) 2484
Hpy166II GTNNAC 9 cut(s) 63, 208, 739, 765, 916, 1184, 1957, 2036, 2393
Hpy8I GTNNAC 9 cut(s) 63, 208, 739, 765, 916, 1184, 1957, 2036, 2393
Hpy99I CGWCG 2 cut(s) 16, 90
HpyAV CCTTC 6 cut(s) 139, 193, 317, 1251, 1858, 2014
HpyCH4III ACNGT 7 cut(s) 1067, 1116, 1162, 1604, 1757, 1826, 2033
HpyCH4IV ACGT 2 cut(s) 844, 1953
HpyF10VI GCNNNNNNNGC 9 cut(s) 13, 621, 1792, 2218, 2297, 2357, 2432, 2453, 2474
HpySE526I ACGT 2 cut(s) 844, 1953
Hsp92I GRCGYC 1 cut(s) 11
HspAI GCGC 1 cut(s) 2221
Kpn2I TCCGGA 1 cut(s) 1660
Ksp22I TGATCA 1 cut(s) 1096
LmnI GCTCC 6 cut(s) 212, 632, 793, 885, 1271, 1690
LweI GCATC 5 cut(s) 1129, 1999, 2188, 2299, 2488
MaeI CTAG 9 cut(s) 383, 591, 1233, 1496, 1652, 1881, 2070, 2250, 2277
MaeII ACGT 2 cut(s) 844, 1953
MaeIII GTNAC 2 cut(s) 527, 1483
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 4 cut(s) 646, 1229, 1883, 2369
MhlI GDGCHC 5 cut(s) 217, 474, 798, 1598, 2407
MmeI TCCRAC 2 cut(s) 624, 1459
Mph1103I ATGCAT 1 cut(s) 966
MroI TCCGGA 1 cut(s) 1660
MroXI GAANNNNTTC 2 cut(s) 666, 1240
MseI TTAA 7 cut(s) 350, 1047, 1178, 1289, 1547, 2000, 2337
MslI CAYNNNNRTG 6 cut(s) 902, 947, 1023, 1781, 1859, 1974
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 5 cut(s) 379, 525, 1124, 1592, 1661
MspR9I CCNGG 8 cut(s) 379, 508, 822, 838, 1593, 1788, 1806, 2174
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 3 cut(s) 1396, 2332, 2428
MvaI CCWGG 6 cut(s) 508, 822, 838, 1788, 1806, 2174
MvnI CGCG 1 cut(s) 1552
MwoI GCNNNNNNNGC 9 cut(s) 13, 621, 1792, 2218, 2297, 2357, 2432, 2453, 2474
NciI CCSGG 2 cut(s) 379, 1593
NdeI CATATG 1 cut(s) 2041
NheI GCTAGC 1 cut(s) 382
NlaIV GGNNCC 5 cut(s) 43, 214, 795, 1273, 1628
NmeAIII GCCGAG 2 cut(s) 141, 694
NmuCI GTSAC 1 cut(s) 527
NsbI TGCGCA 1 cut(s) 2222
NsiI ATGCAT 1 cut(s) 966
NspI RCATGY 4 cut(s) 745, 1022, 1428, 1979
NspV TTCGAA 1 cut(s) 1388
PagI TCATGA 1 cut(s) 1969
PciI ACATGT 2 cut(s) 741, 1975
PctI GAATGC 3 cut(s) 1396, 2332, 2428
PdmI GAANNNNTTC 2 cut(s) 666, 1240
PfeI GAWTC 6 cut(s) 332, 547, 1327, 1349, 1418, 1454
PflFI GACNNNGTC 1 cut(s) 58
PinAI ACCGGT 1 cut(s) 524
PscI ACATGT 2 cut(s) 741, 1975
PshBI ATTAAT 1 cut(s) 2000
Psp124BI GAGCTC 1 cut(s) 2407
Psp6I CCWGG 6 cut(s) 506, 820, 836, 1786, 1804, 2172
PspGI CCWGG 6 cut(s) 506, 820, 836, 1786, 1804, 2172
PspN4I GGNNCC 5 cut(s) 43, 214, 795, 1273, 1628
PspPI GGNCC 8 cut(s) 42, 174, 232, 246, 366, 522, 750, 1626
PsrI GAACNNNNNNTAC 2 cut(s) 473, 505
PstI CTGCAG 4 cut(s) 762, 928, 2302, 2509
PsuI RGATCY 4 cut(s) 646, 1229, 1883, 2369
PsyI GACNNNGTC 1 cut(s) 58
RsaI GTAC 5 cut(s) 575, 740, 843, 2412, 2517
RsaNI GTAC 5 cut(s) 574, 739, 842, 2411, 2516
RseI CAYNNNNRTG 6 cut(s) 902, 947, 1023, 1781, 1859, 1974
Rsr2I CGGWCCG 1 cut(s) 522
RsrII CGGWCCG 1 cut(s) 522
SacI GAGCTC 1 cut(s) 2407
SalI GTCGAC 1 cut(s) 61
SaqAI TTAA 7 cut(s) 350, 1047, 1178, 1289, 1547, 2000, 2337
Sau96I GGNCC 8 cut(s) 42, 174, 232, 246, 366, 522, 750, 1626
ScrFI CCNGG 8 cut(s) 379, 508, 822, 838, 1593, 1788, 1806, 2174
SduI GDGCHC 5 cut(s) 217, 474, 798, 1598, 2407
SfaNI GCATC 5 cut(s) 1129, 1999, 2188, 2299, 2488
SfcI CTRYAG 5 cut(s) 758, 924, 1317, 2298, 2505
SfuI TTCGAA 1 cut(s) 1388
SinI GGWCC 6 cut(s) 42, 174, 366, 522, 750, 1626
SmiMI CAYNNNNRTG 6 cut(s) 902, 947, 1023, 1781, 1859, 1974
SmlI CTYRAG 2 cut(s) 434, 473
SmoI CTYRAG 2 cut(s) 434, 473
SsiI CCGC 5 cut(s) 235, 238, 386, 618, 1394
SspMI CTAG 9 cut(s) 383, 591, 1233, 1496, 1652, 1881, 2070, 2250, 2277
SstI GAGCTC 1 cut(s) 2407
StyD4I CCNGG 8 cut(s) 377, 506, 820, 836, 1591, 1786, 1804, 2172
StyI CCWWGG 1 cut(s) 1275
TaaI ACNGT 7 cut(s) 1067, 1116, 1162, 1604, 1757, 1826, 2033
TaiI ACGT 2 cut(s) 847, 1956
TaqII GACCGA 1 cut(s) 409
TatI WGTACW 2 cut(s) 738, 2515
TauI GCSGC 3 cut(s) 237, 240, 621
TfiI GAWTC 6 cut(s) 332, 547, 1327, 1349, 1418, 1454
Tru1I TTAA 7 cut(s) 350, 1047, 1178, 1289, 1547, 2000, 2337
Tru9I TTAA 7 cut(s) 350, 1047, 1178, 1289, 1547, 2000, 2337
TscAI CASTG 6 cut(s) 1058, 1167, 1386, 1438, 1771, 2202
TseFI GTSAC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 6 cut(s) 678, 886, 1203, 1723, 1986, 2144
TspGWI ACGGA 5 cut(s) 225, 535, 1273, 1821, 2378
TspRI CASTG 6 cut(s) 1058, 1167, 1386, 1438, 1771, 2202
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 6 cut(s) 42, 174, 366, 522, 750, 1626
VspI ATTAAT 1 cut(s) 2000
XagI CCTNNNNNAGG 1 cut(s) 1785
XapI RAATTY 2 cut(s) 136, 1991
XbaI TCTAGA 2 cut(s) 1232, 1880
XceI RCATGY 4 cut(s) 745, 1022, 1428, 1979
XmiI GTMKAC 1 cut(s) 62
XmnI GAANNNNTTC 2 cut(s) 666, 1240
XspI CTAG 9 cut(s) 383, 591, 1233, 1496, 1652, 1881, 2070, 2250, 2277
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.