Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005430

Physical Location & Seq

Genomic Coordinates Reverse (-)

32918989 .. 32919650

Gene Structure 662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1274700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 288 bp

ATGGCCGCAGTTGGTACAGTCCGCAATAACGAAGGTTTCGCCAACAGCATCGAGACCGAAAGCCTCACTCGCTTCGCTGTTGATGAACACAACAAGAAGGAGAATGCTTTGCTGGAGTTTGTGAGGGTAGTGCATGAAAAGGTACAAGTGGTTTCAGGCTCTCTGCACCATCTGAAAATTGAGGCCACCGATGGCGGAACGAAGAATGTTTATGAAGCTAAGGTGTGGGTAAAGCCATGGGAGAACTTCAAGCAAGTGCAGGAGTTCAAGCTTGTTTTGGACTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

96

Amino Acids

10.77

Weight (kDa)

6.06

Isoelectric Point (pI)

18.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	15 - 92	6.7e-14	Cystatin domain
SQAPI	PF16845	20 - 91	7.5e-20	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40880 AT5G12140
fragaria_vesca	FvH4_7g04180
malus_domestica	MD02G1274700.v1.1 MD07G1042400.v1.1
prunus_persica	Prupe.2G044800_v2.0.a1 Prupe.2G047300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0328521
rosa_laevigata	RLG00000016813
rosa_multiflora	Rmu_sc0000725.1_g000003 Rmu_sc0001563.1_g000009 Rmu_sc0006331.1_g000001 Rmu_sc0016254.1_g000002
rosa_roxburghii	Rroxscaffold_4G00321540
rosa_rugosa	Rorug01G0074300 Rorug01G0074800 Rorug05G0191000
rosa_samantha	Rh1AG092600 Rh1AG092800 Rh1AG093400 Rh1BG072900 Rh1BG074600 Rh1CG089700 Rh1CG090500 Rh1DG095800 Rh1DG096300 Rh1DG096500
rosa_wichuraiana	Rw1G007240 Rw1G007270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	6, 22, 195
AcoI	YGGCCR	1 cut(s)	3
AfaI	GTAC	2 cut(s)	16, 144
AgsI	TTSAA	2 cut(s)	250, 268
AluBI	AGCT	2 cut(s)	218, 271
AluI	AGCT	2 cut(s)	218, 271
Alw26I	GTCTC	1 cut(s)	47
AoxI	GGCC	2 cut(s)	3, 183
BccI	CCATC	2 cut(s)	177, 185
BcoDI	GTCTC	1 cut(s)	47
BfaI	CTAG	1 cut(s)	286
BisI	GCNGC	1 cut(s)	6
BlsI	GCNGC	1 cut(s)	7
BmsI	GCATC	1 cut(s)	57
BpmI	CTGGAG	1 cut(s)	134
Bpu10I	CCTNAGC	1 cut(s)	219
BsaI	GGTCTC	1 cut(s)	47
BsaJI	CCNNGG	1 cut(s)	236
BseDI	CCNNGG	1 cut(s)	236
BsgI	GTGCAG	2 cut(s)	149, 278
BshFI	GGCC	2 cut(s)	5, 185
BsmAI	GTCTC	1 cut(s)	47
BsmI	GAATGC	1 cut(s)	109
BsnI	GGCC	2 cut(s)	5, 185
Bso31I	GGTCTC	1 cut(s)	47
Bsp19I	CCATGG	1 cut(s)	236
BspACI	CCGC	3 cut(s)	6, 22, 195
BspANI	GGCC	2 cut(s)	5, 185
BspTNI	GGTCTC	1 cut(s)	47
BssECI	CCNNGG	1 cut(s)	236
BssT1I	CCWWGG	1 cut(s)	236
Bst4CI	ACNGT	1 cut(s)	19
BstDEI	CTNAG	1 cut(s)	219
BstDSI	CCRYGG	1 cut(s)	236
BstMAI	GTCTC	1 cut(s)	47
BstMWI	GCNNNNNNNGC	1 cut(s)	69
BsuRI	GGCC	2 cut(s)	5, 185
BtgI	CCRYGG	1 cut(s)	236
Csp6I	GTAC	2 cut(s)	15, 143
CviAII	CATG	2 cut(s)	134, 237
CviJI	RGCY	7 cut(s)	5, 63, 159, 185, 218, 235, 271
CviKI_1	RGCY	7 cut(s)	5, 63, 159, 185, 218, 235, 271
CviQI	GTAC	2 cut(s)	15, 143
DdeI	CTNAG	1 cut(s)	219
EaeI	YGGCCR	1 cut(s)	3
EciI	GGCGGA	1 cut(s)	210
Eco130I	CCWWGG	1 cut(s)	236
Eco31I	GGTCTC	1 cut(s)	47
EcoT14I	CCWWGG	1 cut(s)	236
ErhI	CCWWGG	1 cut(s)	236
FaeI	CATG	2 cut(s)	137, 240
FaiI	YATR	3 cut(s)	135, 213, 238
FatI	CATG	2 cut(s)	133, 236
Fnu4HI	GCNGC	1 cut(s)	6
Fsp4HI	GCNGC	1 cut(s)	6
FspBI	CTAG	1 cut(s)	286
GluI	GCNGC	1 cut(s)	6
GsuI	CTGGAG	1 cut(s)	134
HaeIII	GGCC	2 cut(s)	5, 185
Hin1II	CATG	2 cut(s)	137, 240
HindIII	AAGCTT	1 cut(s)	269
HinfI	GANTC	1 cut(s)	281
Hpy188I	TCNGA	1 cut(s)	174
Hpy188III	TCNNGA	1 cut(s)	52
HpyAV	CCTTC	2 cut(s)	26, 91
HpyCH4III	ACNGT	1 cut(s)	19
HpyCH4V	TGCA	3 cut(s)	133, 166, 259
HpyF10VI	GCNNNNNNNGC	1 cut(s)	69
HpyF3I	CTNAG	1 cut(s)	219
Hsp92II	CATG	2 cut(s)	137, 240
LpnPI	CCDG	3 cut(s)	98, 141, 245
LweI	GCATC	1 cut(s)	57
MaeI	CTAG	1 cut(s)	286
MboII	GAAGA	1 cut(s)	214
MluCI	AATT	1 cut(s)	177
MlyI	GAGTC	1 cut(s)	275
MnlI	CCTC	3 cut(s)	74, 117, 175
Mva1269I	GAATGC	1 cut(s)	109
MwoI	GCNNNNNNNGC	1 cut(s)	69
NcoI	CCATGG	1 cut(s)	236
NlaIII	CATG	2 cut(s)	137, 240
PctI	GAATGC	1 cut(s)	109
PkrI	GCNGC	1 cut(s)	7
PleI	GAGTC	1 cut(s)	275
PpsI	GAGTC	1 cut(s)	275
RsaI	GTAC	2 cut(s)	16, 144
RsaNI	GTAC	2 cut(s)	15, 143
SatI	GCNGC	1 cut(s)	6
SchI	GAGTC	1 cut(s)	275
SetI	ASST	5 cut(s)	37, 144, 220, 225, 273
SfaNI	GCATC	1 cut(s)	57
Sse9I	AATT	1 cut(s)	177
SsiI	CCGC	3 cut(s)	6, 22, 195
SspMI	CTAG	1 cut(s)	286
StyI	CCWWGG	1 cut(s)	236
TaaI	ACNGT	1 cut(s)	19
TaqI	TCGA	1 cut(s)	51
TaqII	GACCGA	1 cut(s)	71
TasI	AATT	1 cut(s)	177
TauI	GCSGC	1 cut(s)	8
TspDTI	ATGAA	3 cut(s)	99, 150, 228
XspI	CTAG	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.