Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0005430

Physical Location & Seq

Genomic Coordinates Forward (+)

10275909 .. 10277408

Gene Structure 1500 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016813

Sequence Viewer

CDS Protein Promoter (2k)

Length: 390 bp

ATGGCCACCCTCGGTGGAATTCGTGACTCCCCTGCCGGCTTCGAGAACAGCCTCGAGACCGAATCCCTCGTTCGCTTCGCCGTCGACGAGCATAACAAGAAGGAATTTGTTCGAGTGGTGAAGGCTAAGGAACAGGTGGTTGCCAGCACTCTGCACCATCTGGTTATGGAAGCCATCAACAGTGCCAAGAAGAAGCTCTATGAAGCCAAGGTTTGGGTTAAGCCTTGGTTGGGCTTTAAACAAGTTCAGGAATTCAAGCACGCTGAGGATCCCGAAGCTGCATCTGAAACGTCATCGTTTACTGCTTCCGATTTTTGCATTAAGCAAGGTAGTGATATGACATCAATCAACACGAACATTTGCCCCTGCCTGGATGAGCTTGGAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009892 GO:0010035 GO:0010466 GO:0010605 GO:0010951 GO:0012505 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0042221 GO:0043086 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050897 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

130

Amino Acids

14.29

Weight (kDa)

5.56

Isoelectric Point (pI)

30.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	14 - 87	4.6e-11	Cystatin domain
SQAPI	PF16845	22 - 87	7.6e-13	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40880 AT5G12140
fragaria_vesca	FvH4_7g04180
malus_domestica	MD02G1274700.v1.1 MD07G1042400.v1.1
prunus_persica	Prupe.2G044800_v2.0.a1 Prupe.2G047300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0328521
rosa_laevigata	RLG00000016813
rosa_multiflora	Rmu_sc0000725.1_g000003 Rmu_sc0001563.1_g000009 Rmu_sc0006331.1_g000001 Rmu_sc0016254.1_g000002
rosa_roxburghii	Rroxscaffold_4G00321540
rosa_rugosa	Rorug01G0074300 Rorug01G0074800 Rorug05G0191000
rosa_samantha	Rh1AG092600 Rh1AG092800 Rh1AG093400 Rh1BG072900 Rh1BG074600 Rh1CG089700 Rh1CG090500 Rh1DG095800 Rh1DG096300 Rh1DG096500
rosa_wichuraiana	Rw1G007240 Rw1G007270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	213
AccI	GTMKAC	1 cut(s)	84
AclWI	GGATC	2 cut(s)	263, 276
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	18, 104, 251
AfiI	CCNNNNNNNGG	3 cut(s)	213, 230, 370
AgsI	TTSAA	1 cut(s)	256
AjnI	CCWGG	1 cut(s)	369
AluBI	AGCT	4 cut(s)	196, 278, 379, 386
AluI	AGCT	4 cut(s)	196, 278, 379, 386
Alw26I	GTCTC	1 cut(s)	50
AlwI	GGATC	2 cut(s)	263, 276
Ama87I	CYCGRG	1 cut(s)	53
AoxI	GGCC	1 cut(s)	3
ApeKI	GCWGC	1 cut(s)	278
ApoI	RAATTY	3 cut(s)	18, 104, 251
Asp700I	GAANNNNTTC	1 cut(s)	108
AsuHPI	GGTGA	1 cut(s)	130
AvaI	CYCGRG	1 cut(s)	53
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	268
BbvCI	CCTCAGC	1 cut(s)	264
BbvI	GCAGC	1 cut(s)	265
BccI	CCATC	2 cut(s)	165, 182
BceAI	ACGGC	1 cut(s)	65
BciT130I	CCWGG	1 cut(s)	371
BcoDI	GTCTC	1 cut(s)	50
BisI	GCNGC	1 cut(s)	279
BlsI	GCNGC	1 cut(s)	280
Bme1390I	CCNGG	1 cut(s)	371
BmeT110I	CYCGRG	1 cut(s)	53
BmiI	GGNNCC	1 cut(s)	270
BmrFI	CCNGG	1 cut(s)	371
BmsI	GCATC	1 cut(s)	290
Bpu10I	CCTNAGC	2 cut(s)	126, 264
BsaI	GGTCTC	1 cut(s)	50
BsaJI	CCNNGG	3 cut(s)	10, 207, 224
Bsc4I	CCNNNNNNNGG	3 cut(s)	213, 230, 370
Bse118I	RCCGGY	1 cut(s)	35
BseBI	CCWGG	1 cut(s)	371
BseDI	CCNNGG	3 cut(s)	10, 207, 224
BseGI	GGATG	1 cut(s)	379
BseLI	CCNNNNNNNGG	3 cut(s)	213, 230, 370
BseMII	CTCAG	1 cut(s)	255
BseXI	GCAGC	1 cut(s)	265
BsgI	GTGCAG	1 cut(s)	137
BshFI	GGCC	1 cut(s)	5
BsiHKCI	CYCGRG	1 cut(s)	53
BsiSI	CCGG	1 cut(s)	36
BslI	CCNNNNNNNGG	3 cut(s)	213, 230, 370
BsmAI	GTCTC	1 cut(s)	50
BsnI	GGCC	1 cut(s)	5
Bso31I	GGTCTC	1 cut(s)	50
BsoBI	CYCGRG	1 cut(s)	53
Bsp143I	GATC	1 cut(s)	268
BspANI	GGCC	1 cut(s)	5
BspCNI	CTCAG	1 cut(s)	256
BspLI	GGNNCC	1 cut(s)	270
BspPI	GGATC	2 cut(s)	263, 276
BspTNI	GGTCTC	1 cut(s)	50
BsrFI	RCCGGY	1 cut(s)	35
BssAI	RCCGGY	1 cut(s)	35
BssECI	CCNNGG	3 cut(s)	10, 207, 224
BssMI	GATC	1 cut(s)	268
BssT1I	CCWWGG	2 cut(s)	207, 224
Bst2UI	CCWGG	1 cut(s)	371
Bst4CI	ACNGT	1 cut(s)	182
BstC8I	GCNNGC	3 cut(s)	37, 145, 261
BstDEI	CTNAG	2 cut(s)	126, 264
BstF5I	GGATG	1 cut(s)	379
BstKTI	GATC	1 cut(s)	271
BstMAI	GTCTC	1 cut(s)	50
BstMBI	GATC	1 cut(s)	268
BstNI	CCWGG	1 cut(s)	371
BstSCI	CCNGG	1 cut(s)	369
BstV1I	GCAGC	1 cut(s)	265
BstX2I	RGATCY	1 cut(s)	268
BstYI	RGATCY	1 cut(s)	268
BsuRI	GGCC	1 cut(s)	5
BtsCI	GGATG	1 cut(s)	379
BtsIMutI	CAGTG	1 cut(s)	187
Cac8I	GCNNGC	3 cut(s)	37, 145, 261
Cfr10I	RCCGGY	1 cut(s)	35
DdeI	CTNAG	2 cut(s)	126, 264
DpnI	GATC	1 cut(s)	270
DpnII	GATC	1 cut(s)	268
DraI	TTTAAA	1 cut(s)	238
EaeI	YGGCCR	1 cut(s)	3
Eco130I	CCWWGG	2 cut(s)	207, 224
Eco31I	GGTCTC	1 cut(s)	50
Eco88I	CYCGRG	1 cut(s)	53
EcoRI	GAATTC	2 cut(s)	18, 251
EcoRII	CCWGG	1 cut(s)	369
EcoT14I	CCWWGG	2 cut(s)	207, 224
ErhI	CCWWGG	2 cut(s)	207, 224
FaiI	YATR	4 cut(s)	93, 167, 201, 338
FblI	GTMKAC	1 cut(s)	84
Fnu4HI	GCNGC	1 cut(s)	279
FokI	GGATG	1 cut(s)	386
Fsp4HI	GCNGC	1 cut(s)	279
GluI	GCNGC	1 cut(s)	279
HaeIII	GGCC	1 cut(s)	5
HapII	CCGG	1 cut(s)	36
HincII	GTYRAC	1 cut(s)	85
HindII	GTYRAC	1 cut(s)	85
HinfI	GANTC	2 cut(s)	26, 62
HpaII	CCGG	1 cut(s)	36
HphI	GGTGA	1 cut(s)	130
Hpy166II	GTNNAC	2 cut(s)	85, 300
Hpy188I	TCNGA	2 cut(s)	286, 310
Hpy188III	TCNNGA	5 cut(s)	23, 43, 55, 248, 272
Hpy8I	GTNNAC	2 cut(s)	85, 300
Hpy99I	CGWCG	2 cut(s)	86, 89
HpyAV	CCTTC	2 cut(s)	94, 115
HpyCH4III	ACNGT	1 cut(s)	182
HpyCH4IV	ACGT	1 cut(s)	290
HpyCH4V	TGCA	3 cut(s)	154, 281, 318
HpyF3I	CTNAG	2 cut(s)	126, 264
HpySE526I	ACGT	1 cut(s)	290
KroI	GCCGGC	1 cut(s)	35
KroNI	GCCGGC	1 cut(s)	37
Kzo9I	GATC	1 cut(s)	268
LmnI	GCTCC	1 cut(s)	383
LpnPI	CCDG	9 cut(s)	45, 49, 119, 146, 157, 233, 356, 379, 383
Lsp1109I	GCAGC	1 cut(s)	265
LweI	GCATC	1 cut(s)	290
MaeII	ACGT	1 cut(s)	290
MaeIII	GTNAC	1 cut(s)	23
MalI	GATC	1 cut(s)	270
MboI	GATC	1 cut(s)	268
MboII	GAAGA	1 cut(s)	202
MflI	RGATCY	1 cut(s)	268
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	3 cut(s)	18, 104, 251
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	20
MnlI	CCTC	4 cut(s)	20, 62, 77, 259
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	35
MroXI	GAANNNNTTC	1 cut(s)	108
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	219, 237, 321
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	1 cut(s)	36
MspR9I	CCNGG	1 cut(s)	371
MvaI	CCWGG	1 cut(s)	371
NaeI	GCCGGC	1 cut(s)	37
NdeII	GATC	1 cut(s)	268
NgoMIV	GCCGGC	1 cut(s)	35
NlaIV	GGNNCC	1 cut(s)	270
NmuCI	GTSAC	1 cut(s)	23
PaeR7I	CTCGAG	1 cut(s)	53
PcsI	WCGNNNNNNNCGW	1 cut(s)	84
PdiI	GCCGGC	1 cut(s)	37
PdmI	GAANNNNTTC	1 cut(s)	108
PfeI	GAWTC	1 cut(s)	62
PflMI	CCANNNNNTGG	1 cut(s)	213
PkrI	GCNGC	1 cut(s)	280
PleI	GAGTC	1 cut(s)	20
PpsI	GAGTC	1 cut(s)	20
Psp6I	CCWGG	1 cut(s)	369
PspGI	CCWGG	1 cut(s)	369
PspN4I	GGNNCC	1 cut(s)	270
PsuI	RGATCY	1 cut(s)	268
SalI	GTCGAC	1 cut(s)	83
SaqAI	TTAA	3 cut(s)	219, 237, 321
SatI	GCNGC	1 cut(s)	279
Sau3AI	GATC	1 cut(s)	268
SchI	GAGTC	1 cut(s)	20
ScrFI	CCNGG	1 cut(s)	371
SetI	ASST	8 cut(s)	138, 198, 213, 280, 293, 331, 381, 388
SfaNI	GCATC	1 cut(s)	290
Sfr274I	CTCGAG	1 cut(s)	53
SgrDI	CGTCGACG	1 cut(s)	83
SlaI	CTCGAG	1 cut(s)	53
SmlI	CTYRAG	1 cut(s)	53
SmoI	CTYRAG	1 cut(s)	53
Sse9I	AATT	3 cut(s)	18, 104, 251
StyD4I	CCNGG	1 cut(s)	369
StyI	CCWWGG	2 cut(s)	207, 224
TaaI	ACNGT	1 cut(s)	182
TaiI	ACGT	1 cut(s)	293
TaqI	TCGA	4 cut(s)	42, 54, 84, 112
TaqII	GACCGA	1 cut(s)	74
TasI	AATT	3 cut(s)	18, 104, 251
TfiI	GAWTC	1 cut(s)	62
Tru1I	TTAA	3 cut(s)	219, 237, 321
Tru9I	TTAA	3 cut(s)	219, 237, 321
TscAI	CASTG	1 cut(s)	187
TseFI	GTSAC	1 cut(s)	23
TseI	GCWGC	1 cut(s)	278
Tsp45I	GTSAC	1 cut(s)	23
TspDTI	ATGAA	1 cut(s)	216
TspRI	CASTG	1 cut(s)	187
Van91I	CCANNNNNTGG	1 cut(s)	213
XapI	RAATTY	3 cut(s)	18, 104, 251
XhoI	CTCGAG	1 cut(s)	53
XmiI	GTMKAC	1 cut(s)	84
XmnI	GAANNNNTTC	1 cut(s)	108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.