Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005329

Physical Location & Seq

Genomic Coordinates Forward (+)

35667119 .. 35670403

Gene Structure 3285 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1302300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGCCATCACCAGCAGCATATGTTCATCAACAAACCAACCTCCAACTCTCCCAACACTTCCACTCAAAACCCACCACCCTCTCCAACTGCACTTCCCTCCACTCTCTTCCTCTTCCTCCTCCAAATTTGGCGCCAGTTTGGTCTCCAATGACGCATTGGTAGTTGCAGCTGCGGCCGAGGCGCTGGCGCTGGCCCGGGCTGCTTTAGAGGCGGCCAGAGATGCTGCAGCGGCGAGGGATGAGATTGTGAAGGCTTGGAGTTGTAGGGAGAGTGGGAATGGGAGTGGTGGGTTGGTGATGAGGAGGAAGAGGAGGAGGAAAAGGAGAAAAGGGTTGGAGGGTTTGGATGAGGAGAGGGAGAGGGGCGTTGGAGATGGAAAGTTGTCGTTTGGGTTAGTGAGATGTGGGCATCTGAGCTCAAAAGAAGAAGCAGAATGCTGTCTAAGTCTCAAGGAGGGAGCAAGACTAGAATCACGAAGATTAAGAGTCGTAGAAGCTGAAGAGCAGGAGCCGACCTCAAAGCAGTTAGCCAAGGCAATGGGAATGAAGACGAGAAGTATAGATAAGGTGCTTTGTAAGAAAAGAGAGTCACAAGAAAGAATCATCAGGAGCTACCGGGGACTCATTGTCTCTGTTGCCAGCAGTTATCAAGGCAAAGGATTAAGCTTTCAAGACCTCATTCAGGAAGGGAGCATTGGGCTACTTCGAGGGGCAGAGAAGTTTGATCATGAGCGAGGATGTAAGTTGTCAACTTACGTCTACTGGTGGATCAGGCAAGCTATTATCAGAGCCCTAGAGAAGAAGTCTAGAATAATCAGATTACCAGTTAACATGTGCGGTGTGATGGCAAAAATCACAAAAGCTAAAAATTCCTTCAACCAAAGAATACAGCGACTGCCTACATATGACGAAATCGCTCAAGAGGTCAATGTGCATGCTGCAACAGTAAAGCTTGTTTGCGAGAGGAGCAGACCGCCAATTTCATTGGATCAGGTGGTAACTGTTCGGGGTTGCATGTCACTGCAGGAGATCATGCAAGGGCCAGAGGAGATGATGCCAGAAAAGATGCTTACAAAACAGCTAATGAAGCAAGAGGTCGAGAAGCTTCTCAAGACGCTGAGCGATAGAGAAGCATATGTGTTGAGATTACACTTTGGGCTACACGGAGAGACCCCAAAGTCCTTTGAGGACATAGGAAGACTGTTAAAGCTTTCGAGGGAGAGGGTTCGACAGATTAATGGAATTGCGTTATCAAAGTTAAAGCAGACTAGTACATTCGACAATCTAAAATTGTATATTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

435

Amino Acids

48.54

Weight (kDa)

9.77

Isoelectric Point (pI)

46.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	201 - 270	7.4e-22	Sigma-70 region 2
Sigma70_r3	PF04539	283 - 356	1.1e-09	Sigma-70 region 3
Sigma70_r4	PF04545	369 - 422	9.1e-12	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13730
fragaria_vesca	FvH4_7g01630
malus_domestica	MD02G1302300.v1.1 MD07G1021800.v1.1
prunus_persica	Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1
pyrus_communis	pycom02g25460 pycom07g01650
rosa_chinensis	RchiOBHm_Chr1g0317901
rosa_laevigata	RLG00000029590 RLG00000030578
rosa_multiflora	Rmu_sc0000654.1_g000054 Rmu_ssc0000008.1_g000049
rosa_roxburghii	Rroxscaffold_4G00330140
rosa_rugosa	Rorug01G0018500
rosa_samantha	Rh1AG030000 Rh1BG026000 Rh1BG026100 Rh1CG028600 Rh1DG041500
rosa_wichuraiana	Rw1G002260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1177
AccB1I	GGYRCC	1 cut(s)	131
AccI	GTMKAC	1 cut(s)	759
AciI	CCGC	5 cut(s)	173, 212, 230, 837, 974
AclWI	GGATC	2 cut(s)	776, 996
AcoI	YGGCCR	3 cut(s)	3, 174, 213
AcsI	RAATTY	2 cut(s)	125, 868
AcuI	CTGAAG	1 cut(s)	519
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	1 cut(s)	1273
AfiI	CCNNNNNNNGG	1 cut(s)	682
AflIII	ACRYGT	1 cut(s)	831
AgsI	TTSAA	2 cut(s)	671, 877
AhdI	GACNNNNNGTC	1 cut(s)	626
AhlI	ACTAGT	1 cut(s)	1268
AjuI	GAANNNNNNNTTGG	2 cut(s)	678, 710
Alw21I	GWGCWC	1 cut(s)	419
Alw26I	GTCTC	4 cut(s)	148, 452, 634, 1163
AlwI	GGATC	2 cut(s)	776, 996
AlwNI	CAGNNNCTG	1 cut(s)	895
Ama87I	CYCGRG	1 cut(s)	195
AoxI	GGCC	5 cut(s)	3, 174, 192, 213, 1040
ApeKI	GCWGC	7 cut(s)	15, 167, 170, 200, 224, 227, 938
ApoI	RAATTY	2 cut(s)	125, 868
AseI	ATTAAT	1 cut(s)	1236
AspLEI	GCGC	3 cut(s)	134, 184, 190
AspS9I	GGNCC	2 cut(s)	193, 1040
AsuC2I	CCSGG	3 cut(s)	196, 197, 617
AsuHPI	GGTGA	1 cut(s)	307
AvaI	CYCGRG	1 cut(s)	195
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	131
BanII	GRGCYC	2 cut(s)	419, 793
BbsI	GAAGAC	2 cut(s)	554, 1204
Bbv12I	GWGCWC	1 cut(s)	419
BbvI	GCAGC	7 cut(s)	27, 157, 179, 187, 211, 239, 925
BccI	CCATC	3 cut(s)	14, 368, 838
BclI	TGATCA	1 cut(s)	724
BcnI	CCSGG	3 cut(s)	196, 197, 617
BcoDI	GTCTC	4 cut(s)	148, 452, 634, 1163
BcuI	ACTAGT	1 cut(s)	1268
BfaI	CTAG	4 cut(s)	467, 794, 807, 1269
BfmI	CTRYAG	2 cut(s)	225, 1022
BfoI	RGCGCY	3 cut(s)	135, 185, 191
BlpI	GCTNAGC	1 cut(s)	1118
Bme1390I	CCNGG	3 cut(s)	196, 197, 617
BmeRI	GACNNNNNGTC	1 cut(s)	626
BmeT110I	CYCGRG	1 cut(s)	195
BmgT120I	GGNCC	2 cut(s)	193, 1040
BmiI	GGNNCC	2 cut(s)	133, 510
BmrFI	CCNGG	3 cut(s)	196, 197, 617
BmsI	GCATC	4 cut(s)	211, 418, 1044, 1056
BpiI	GAAGAC	2 cut(s)	554, 1204
BplI	GAGNNNNNCTC	2 cut(s)	500, 532
Bpu1102I	GCTNAGC	1 cut(s)	1118
BpuEI	CTTGAG	3 cut(s)	434, 903, 1094
BpuMI	CCSGG	3 cut(s)	196, 197, 617
BsaHI	GRCGYC	1 cut(s)	132
BsaI	GGTCTC	2 cut(s)	148, 1163
BsaJI	CCNNGG	4 cut(s)	177, 195, 531, 616
Bsc4I	CCNNNNNNNGG	1 cut(s)	682
Bse1I	ACTGG	3 cut(s)	135, 767, 824
Bse3DI	GCAATG	1 cut(s)	543
BseDI	CCNNGG	4 cut(s)	177, 195, 531, 616
BseGI	GGATG	3 cut(s)	244, 352, 743
BseLI	CCNNNNNNNGG	1 cut(s)	682
BseMI	GCAATG	1 cut(s)	543
BseMII	CTCAG	2 cut(s)	404, 1109
BseNI	ACTGG	3 cut(s)	135, 767, 824
BseRI	GAGGAG	7 cut(s)	109, 316, 325, 328, 365, 979, 1061
BseX3I	CGGCCG	1 cut(s)	174
BseXI	GCAGC	7 cut(s)	27, 157, 179, 187, 211, 239, 925
BsgI	GTGCAG	1 cut(s)	74
Bsh1285I	CGRYCG	1 cut(s)	177
BshFI	GGCC	5 cut(s)	5, 176, 194, 215, 1042
BshNI	GGYRCC	1 cut(s)	131
BsiEI	CGRYCG	1 cut(s)	177
BsiHKAI	GWGCWC	1 cut(s)	419
BsiHKCI	CYCGRG	1 cut(s)	195
BsiSI	CCGG	2 cut(s)	196, 616
BslFI	GGGAC	1 cut(s)	633
BslI	CCNNNNNNNGG	1 cut(s)	682
BsmAI	GTCTC	4 cut(s)	148, 452, 634, 1163
BsmFI	GGGAC	1 cut(s)	633
BsmI	GAATGC	1 cut(s)	440
BsnI	GGCC	5 cut(s)	5, 176, 194, 215, 1042
Bso31I	GGTCTC	2 cut(s)	148, 1163
BsoBI	CYCGRG	1 cut(s)	195
Bsp1286I	GDGCHC	2 cut(s)	419, 793
Bsp143I	GATC	4 cut(s)	724, 768, 988, 1029
Bsp1720I	GCTNAGC	1 cut(s)	1118
BspACI	CCGC	5 cut(s)	173, 212, 230, 837, 974
BspANI	GGCC	5 cut(s)	5, 176, 194, 215, 1042
BspCNI	CTCAG	2 cut(s)	405, 1110
BspHI	TCATGA	1 cut(s)	727
BspLI	GGNNCC	2 cut(s)	133, 510
BspMAI	CTGCAG	2 cut(s)	229, 1026
BspPI	GGATC	2 cut(s)	776, 996
BspQI	GCTCTTC	1 cut(s)	495
BspT107I	GGYRCC	1 cut(s)	131
BspTNI	GGTCTC	2 cut(s)	148, 1163
BsrDI	GCAATG	1 cut(s)	543
BsrI	ACTGG	3 cut(s)	135, 767, 824
BssECI	CCNNGG	4 cut(s)	177, 195, 531, 616
BssMI	GATC	4 cut(s)	724, 768, 988, 1029
BssNI	GRCGYC	1 cut(s)	132
BssT1I	CCWWGG	1 cut(s)	531
Bst4CI	ACNGT	3 cut(s)	946, 1003, 1203
Bst6I	CTCTTC	4 cut(s)	112, 118, 302, 495
BstACI	GRCGYC	1 cut(s)	132
BstC8I	GCNNGC	5 cut(s)	186, 192, 640, 777, 936
BstDEI	CTNAG	3 cut(s)	413, 443, 1118
BstENI	CCTNNNNNAGG	1 cut(s)	680
BstF5I	GGATG	3 cut(s)	244, 352, 743
BstH2I	RGCGCY	3 cut(s)	135, 185, 191
BstHHI	GCGC	3 cut(s)	134, 184, 190
BstKTI	GATC	4 cut(s)	727, 771, 991, 1032
BstMAI	GTCTC	4 cut(s)	148, 452, 634, 1163
BstMBI	GATC	4 cut(s)	724, 768, 988, 1029
BstMCI	CGRYCG	1 cut(s)	177
BstMWI	GCNNNNNNNGC	8 cut(s)	173, 179, 200, 209, 221, 230, 966, 1087
BstNSI	RCATGY	3 cut(s)	835, 938, 1018
BstSCI	CCNGG	3 cut(s)	194, 195, 615
BstSFI	CTRYAG	2 cut(s)	225, 1022
BstV1I	GCAGC	7 cut(s)	27, 157, 179, 187, 211, 239, 925
BstV2I	GAAGAC	2 cut(s)	554, 1204
BstXI	CCANNNNNNTGG	1 cut(s)	538
BstZI	CGGCCG	1 cut(s)	174
BsuRI	GGCC	5 cut(s)	5, 176, 194, 215, 1042
BtsCI	GGATG	3 cut(s)	244, 352, 743
BtsI	GCAGTG	1 cut(s)	1019
BtsIMutI	CAGTG	1 cut(s)	1019
Cac8I	GCNNGC	5 cut(s)	186, 192, 640, 777, 936
CaiI	CAGNNNCTG	1 cut(s)	895
CciI	TCATGA	1 cut(s)	727
CfoI	GCGC	3 cut(s)	134, 184, 190
Cfr13I	GGNCC	2 cut(s)	193, 1040
Cfr9I	CCCGGG	1 cut(s)	195
CseI	GACGC	2 cut(s)	161, 1123
Csp6I	GTAC	1 cut(s)	1272
CviAII	CATG	5 cut(s)	728, 832, 935, 1015, 1033
CviQI	GTAC	1 cut(s)	1272
DdeI	CTNAG	3 cut(s)	413, 443, 1118
DinI	GGCGCC	1 cut(s)	133
DpnI	GATC	4 cut(s)	726, 770, 990, 1031
DpnII	GATC	4 cut(s)	724, 768, 988, 1029
DrdI	GACNNNNNNGTC	1 cut(s)	1177
DriI	GACNNNNNGTC	1 cut(s)	626
DseDI	GACNNNNNNGTC	1 cut(s)	1177
EaeI	YGGCCR	3 cut(s)	3, 174, 213
EagI	CGGCCG	1 cut(s)	174
Eam1104I	CTCTTC	4 cut(s)	112, 118, 302, 495
Eam1105I	GACNNNNNGTC	1 cut(s)	626
EarI	CTCTTC	4 cut(s)	112, 118, 302, 495
Ecl136II	GAGCTC	1 cut(s)	417
EclXI	CGGCCG	1 cut(s)	174
Eco130I	CCWWGG	1 cut(s)	531
Eco24I	GRGCYC	2 cut(s)	419, 793
Eco31I	GGTCTC	2 cut(s)	148, 1163
Eco52I	CGGCCG	1 cut(s)	174
Eco53kI	GAGCTC	1 cut(s)	417
Eco57I	CTGAAG	1 cut(s)	519
Eco88I	CYCGRG	1 cut(s)	195
EcoICRI	GAGCTC	1 cut(s)	417
EcoNI	CCTNNNNNAGG	1 cut(s)	680
EcoT14I	CCWWGG	1 cut(s)	531
EcoT38I	GRGCYC	2 cut(s)	419, 793
EgeI	GGCGCC	1 cut(s)	133
EheI	GGCGCC	1 cut(s)	133
ErhI	CCWWGG	1 cut(s)	531
FaeI	CATG	5 cut(s)	731, 835, 938, 1018, 1036
FaqI	GGGAC	1 cut(s)	633
FatI	CATG	5 cut(s)	727, 831, 934, 1014, 1032
FauNDI	CATATG	3 cut(s)	20, 904, 1135
FbaI	TGATCA	1 cut(s)	724
FblI	GTMKAC	1 cut(s)	759
FokI	GGATG	3 cut(s)	251, 359, 750
FriOI	GRGCYC	2 cut(s)	419, 793
FspBI	CTAG	4 cut(s)	467, 794, 807, 1269
GlaI	GCGC	3 cut(s)	133, 183, 189
HaeII	RGCGCY	3 cut(s)	135, 185, 191
HaeIII	GGCC	5 cut(s)	5, 176, 194, 215, 1042
HapII	CCGG	2 cut(s)	196, 616
HgaI	GACGC	2 cut(s)	161, 1123
HhaI	GCGC	3 cut(s)	134, 184, 190
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	5 cut(s)	731, 835, 938, 1018, 1036
Hin6I	GCGC	3 cut(s)	132, 182, 188
HinP1I	GCGC	3 cut(s)	132, 182, 188
HincII	GTYRAC	2 cut(s)	750, 829
HindII	GTYRAC	2 cut(s)	750, 829
HindIII	AAGCTT	4 cut(s)	664, 950, 1103, 1208
HinfI	GANTC	5 cut(s)	470, 486, 587, 600, 621
HpaI	GTTAAC	1 cut(s)	829
HpaII	CCGG	2 cut(s)	196, 616
HphI	GGTGA	1 cut(s)	307
Hpy166II	GTNNAC	3 cut(s)	750, 760, 829
Hpy188I	TCNGA	3 cut(s)	414, 788, 818
Hpy188III	TCNNGA	9 cut(s)	474, 607, 671, 683, 728, 807, 920, 1099, 1111
Hpy8I	GTNNAC	3 cut(s)	750, 760, 829
HpyAV	CCTTC	3 cut(s)	244, 680, 883
HpyCH4III	ACNGT	3 cut(s)	946, 1003, 1203
HpyCH4IV	ACGT	1 cut(s)	756
HpyCH4V	TGCA	8 cut(s)	91, 167, 227, 934, 941, 1014, 1024, 1036
HpyF10VI	GCNNNNNNNGC	8 cut(s)	173, 179, 200, 209, 221, 230, 966, 1087
HpyF3I	CTNAG	3 cut(s)	413, 443, 1118
HpySE526I	ACGT	1 cut(s)	756
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	5 cut(s)	731, 835, 938, 1018, 1036
HspAI	GCGC	3 cut(s)	132, 182, 188
KasI	GGCGCC	1 cut(s)	131
Ksp22I	TGATCA	1 cut(s)	724
KspAI	GTTAAC	1 cut(s)	829
Kzo9I	GATC	4 cut(s)	724, 768, 988, 1029
LguI	GCTCTTC	1 cut(s)	495
LmnI	GCTCC	5 cut(s)	458, 508, 609, 690, 966
Lsp1109I	GCAGC	7 cut(s)	27, 157, 179, 187, 211, 239, 925
LweI	GCATC	4 cut(s)	211, 418, 1044, 1056
MaeI	CTAG	4 cut(s)	467, 794, 807, 1269
MaeII	ACGT	1 cut(s)	756
MaeIII	GTNAC	3 cut(s)	588, 997, 1017
MalI	GATC	4 cut(s)	726, 770, 990, 1031
MboI	GATC	4 cut(s)	724, 768, 988, 1029
MboII	GAAGA	9 cut(s)	99, 105, 319, 437, 489, 512, 559, 811, 1209
MhlI	GDGCHC	2 cut(s)	419, 793
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	125, 868, 978, 1242, 1289
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	132
MlyI	GAGTC	3 cut(s)	495, 596, 615
MmeI	TCCRAC	4 cut(s)	68, 109, 315, 349
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	482, 662, 828, 1205, 1236, 1259
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	170, 230
MspI	CCGG	2 cut(s)	196, 616
MspR9I	CCNGG	3 cut(s)	196, 197, 617
Mva1269I	GAATGC	1 cut(s)	440
MwoI	GCNNNNNNNGC	8 cut(s)	173, 179, 200, 209, 221, 230, 966, 1087
NarI	GGCGCC	1 cut(s)	132
NciI	CCSGG	3 cut(s)	196, 197, 617
NdeI	CATATG	3 cut(s)	20, 904, 1135
NdeII	GATC	4 cut(s)	724, 768, 988, 1029
NlaIII	CATG	5 cut(s)	731, 835, 938, 1018, 1036
NlaIV	GGNNCC	2 cut(s)	133, 510
NmeAIII	GCCGAG	1 cut(s)	202
NmuCI	GTSAC	2 cut(s)	588, 1017
NspI	RCATGY	3 cut(s)	835, 938, 1018
PaeI	GCATGC	1 cut(s)	938
PagI	TCATGA	1 cut(s)	727
PciI	ACATGT	1 cut(s)	831
PciSI	GCTCTTC	1 cut(s)	495
PctI	GAATGC	1 cut(s)	440
PfeI	GAWTC	2 cut(s)	470, 600
PleI	GAGTC	3 cut(s)	494, 595, 615
PluTI	GGCGCC	1 cut(s)	135
PpsI	GAGTC	3 cut(s)	494, 595, 615
PscI	ACATGT	1 cut(s)	831
PshBI	ATTAAT	1 cut(s)	1236
Psp124BI	GAGCTC	1 cut(s)	419
PspN4I	GGNNCC	2 cut(s)	133, 510
PspPI	GGNCC	2 cut(s)	193, 1040
PstI	CTGCAG	2 cut(s)	229, 1026
PstNI	CAGNNNCTG	1 cut(s)	895
PvuII	CAGCTG	1 cut(s)	170
RsaI	GTAC	1 cut(s)	1273
RsaNI	GTAC	1 cut(s)	1272
SacI	GAGCTC	1 cut(s)	419
SapI	GCTCTTC	1 cut(s)	495
SaqAI	TTAA	6 cut(s)	482, 662, 828, 1205, 1236, 1259
Sau3AI	GATC	4 cut(s)	724, 768, 988, 1029
Sau96I	GGNCC	2 cut(s)	193, 1040
SchI	GAGTC	3 cut(s)	495, 596, 615
ScrFI	CCNGG	3 cut(s)	196, 197, 617
SduI	GDGCHC	2 cut(s)	419, 793
SfaNI	GCATC	4 cut(s)	211, 418, 1044, 1056
SfcI	CTRYAG	2 cut(s)	225, 1022
SfoI	GGCGCC	1 cut(s)	133
SmaI	CCCGGG	1 cut(s)	197
SmlI	CTYRAG	3 cut(s)	449, 918, 1109
SmoI	CTYRAG	3 cut(s)	449, 918, 1109
SpeI	ACTAGT	1 cut(s)	1268
SphI	GCATGC	1 cut(s)	938
SrfI	GCCCGGGC	1 cut(s)	197
Sse9I	AATT	5 cut(s)	125, 868, 978, 1242, 1289
SsiI	CCGC	5 cut(s)	173, 212, 230, 837, 974
SspDI	GGCGCC	1 cut(s)	131
SspMI	CTAG	4 cut(s)	467, 794, 807, 1269
SstI	GAGCTC	1 cut(s)	419
StyD4I	CCNGG	3 cut(s)	194, 195, 615
StyI	CCWWGG	1 cut(s)	531
TaaI	ACNGT	3 cut(s)	946, 1003, 1203
TaiI	ACGT	1 cut(s)	759
TaqI	TCGA	5 cut(s)	706, 1098, 1214, 1228, 1278
TasI	AATT	5 cut(s)	125, 868, 978, 1242, 1289
TatI	WGTACW	1 cut(s)	1271
TauI	GCSGC	3 cut(s)	176, 215, 233
TfiI	GAWTC	2 cut(s)	470, 600
Tru1I	TTAA	6 cut(s)	482, 662, 828, 1205, 1236, 1259
Tru9I	TTAA	6 cut(s)	482, 662, 828, 1205, 1236, 1259
TscAI	CASTG	1 cut(s)	1026
TseFI	GTSAC	2 cut(s)	588, 1017
TseI	GCWGC	7 cut(s)	15, 167, 170, 200, 224, 227, 938
Tsp45I	GTSAC	2 cut(s)	588, 1017
TspDTI	ATGAA	4 cut(s)	15, 560, 972, 1100
TspGWI	ACGGA	1 cut(s)	1179
TspMI	CCCGGG	1 cut(s)	195
TspRI	CASTG	1 cut(s)	1026
VspI	ATTAAT	1 cut(s)	1236
XagI	CCTNNNNNAGG	1 cut(s)	680
XapI	RAATTY	2 cut(s)	125, 868
XbaI	TCTAGA	1 cut(s)	806
XceI	RCATGY	3 cut(s)	835, 938, 1018
XcmI	CCANNNNNNNNNTGG	1 cut(s)	154
XmaI	CCCGGG	1 cut(s)	195
XmiI	GTMKAC	1 cut(s)	759
XspI	CTAG	4 cut(s)	467, 794, 807, 1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.