Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0005329

Physical Location & Seq

Genomic Coordinates Forward (+)

23375612 .. 23379168

Gene Structure 3557 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g25460.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1053 bp

ATGGCCATCACCAGCAGCATATGTTCATCACCAAACCAATCTCCAACTCTCCCAACACTTCCCCTCAAAACCCACCACCCTCTCCAACTGCACTTCCCTCCACTCTCTTCCTCTTCCTCCTCCAAATTTGGCGCCAGTTTGGTCTCCAATGACACATTGGTAGTTGCAGCTGCGGCCGAGGCGCTGGCGCTGGCCCGGGCTGCTGTAGAGGCCGCCAGAGATGCTGCAGCGGCGAGGGATGAGATTGTGAAGGCTTGGAGTTGTAGGGAGAGTGGGAATGGGAGTGGTGGGTTGGTGATGAGGAGGAAGAGGAGGAGGCAAAGGAGGAGAGGGTTGGAGGGTTTGGATGAGGAGAGGGAGAGGGGCGTTGGAGATGGAAAGTTGTCGTTTGGGTTAGTGAGATGTGGGCATCTGAGCTCAAAAGAAGAAGCAGAATGCTGTCTAAGTCTCAAGGAGGGAGCAAGACTAGAATCACGAAGATTAAGAGTCGTAGAAGTTGAAGAGCAGGAGCTGACCTCAAAGCAGTTAGCCAAGGCAATGGGAATGAAGACGAGAAGTATAGATAAGGTGCTTTGTAAGAAAAGAGAGTCACAAGAAAGAATCATCAGGAGCTACCGGGGACTCATTGTCTCTGTTGCCAGCAGTTATCAAGGCAAAGGATTAAGCTTTCAAGACCTCATTCAGGAAGGGAGCATTGGGCTACTTCGAGGGGCAGAGAAGTTTGATCATGAACGAGGATGTAAGTTATCAACTTACGTCTACTGGTGGATCAGGCAAGCTATTATCAGAGCCCTAGACAAGAAGTCTAGAATAATCAGATTACCAGTAAACGTGTGCGGTATGATGGCAAAAATCACAAAAGCTAAAAATTCCTTCAACCAAAGAATACAGCGACTGCCTACATATGATGAAATTGCCCAAGAGGTCAATGTGCATGCTGCAACAGTAAAGCTTGTTTGTGAGAGGAGCAGACCGCCAATTTCATTGGATCGAGTGGTAACTGTTCGGGGTTGCATGTCACTGCAGCGATCATGCAAGGGCCAGAGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

351

Amino Acids

38.86

Weight (kDa)

10.03

Isoelectric Point (pI)

55.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13730
fragaria_vesca	FvH4_7g01630
malus_domestica	MD02G1302300.v1.1 MD07G1021800.v1.1
prunus_persica	Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1
pyrus_communis	pycom02g25460 pycom07g01650
rosa_chinensis	RchiOBHm_Chr1g0317901
rosa_laevigata	RLG00000029590 RLG00000030578
rosa_multiflora	Rmu_sc0000654.1_g000054 Rmu_ssc0000008.1_g000049
rosa_roxburghii	Rroxscaffold_4G00330140
rosa_rugosa	Rorug01G0018500
rosa_samantha	Rh1AG030000 Rh1BG026000 Rh1BG026100 Rh1CG028600 Rh1DG041500
rosa_wichuraiana	Rw1G002260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	131
AccI	GTMKAC	1 cut(s)	759
AciI	CCGC	5 cut(s)	173, 213, 230, 837, 974
AclWI	GGATC	2 cut(s)	776, 996
AcoI	YGGCCR	2 cut(s)	3, 174
AcsI	RAATTY	2 cut(s)	125, 868
AcyI	GRCGYC	1 cut(s)	132
AfiI	CCNNNNNNNGG	1 cut(s)	682
AflIII	ACRYGT	1 cut(s)	831
AgsI	TTSAA	3 cut(s)	500, 671, 877
AhdI	GACNNNNNGTC	2 cut(s)	626, 802
AjuI	GAANNNNNNNTTGG	2 cut(s)	678, 710
AluBI	AGCT	8 cut(s)	170, 417, 511, 612, 666, 779, 863, 952
AluI	AGCT	8 cut(s)	170, 417, 511, 612, 666, 779, 863, 952
Alw21I	GWGCWC	1 cut(s)	419
Alw26I	GTCTC	3 cut(s)	148, 452, 634
AlwI	GGATC	2 cut(s)	776, 996
AlwNI	CAGNNNCTG	2 cut(s)	511, 895
Ama87I	CYCGRG	1 cut(s)	195
AoxI	GGCC	5 cut(s)	3, 174, 192, 210, 1039
ApeKI	GCWGC	8 cut(s)	15, 167, 170, 200, 224, 227, 938, 1024
ApoI	RAATTY	2 cut(s)	125, 868
AspLEI	GCGC	3 cut(s)	134, 184, 190
AspS9I	GGNCC	2 cut(s)	193, 1039
AsuC2I	CCSGG	3 cut(s)	196, 197, 617
AsuHPI	GGTGA	2 cut(s)	21, 307
AvaI	CYCGRG	1 cut(s)	195
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	131
BanII	GRGCYC	2 cut(s)	419, 793
BbsI	GAAGAC	1 cut(s)	554
Bbv12I	GWGCWC	1 cut(s)	419
BbvI	GCAGC	8 cut(s)	27, 157, 179, 187, 211, 239, 925, 1036
BccI	CCATC	3 cut(s)	14, 368, 838
BclI	TGATCA	1 cut(s)	724
BcnI	CCSGG	3 cut(s)	196, 197, 617
BcoDI	GTCTC	3 cut(s)	148, 452, 634
BfaI	CTAG	3 cut(s)	467, 794, 807
BfmI	CTRYAG	3 cut(s)	204, 225, 1022
BfoI	RGCGCY	3 cut(s)	135, 185, 191
Bme1390I	CCNGG	3 cut(s)	196, 197, 617
BmeRI	GACNNNNNGTC	2 cut(s)	626, 802
BmeT110I	CYCGRG	1 cut(s)	195
BmgT120I	GGNCC	2 cut(s)	193, 1039
BmiI	GGNNCC	1 cut(s)	133
BmrFI	CCNGG	3 cut(s)	196, 197, 617
BmsI	GCATC	2 cut(s)	211, 418
BpiI	GAAGAC	1 cut(s)	554
BplI	GAGNNNNNCTC	2 cut(s)	500, 532
BpuEI	CTTGAG	1 cut(s)	434
BpuMI	CCSGG	3 cut(s)	196, 197, 617
BsaHI	GRCGYC	1 cut(s)	132
BsaI	GGTCTC	1 cut(s)	148
BsaJI	CCNNGG	4 cut(s)	177, 195, 531, 616
Bsc4I	CCNNNNNNNGG	1 cut(s)	682
Bse1I	ACTGG	3 cut(s)	135, 767, 824
Bse3DI	GCAATG	1 cut(s)	543
BseDI	CCNNGG	4 cut(s)	177, 195, 531, 616
BseGI	GGATG	3 cut(s)	244, 352, 743
BseLI	CCNNNNNNNGG	1 cut(s)	682
BseMI	GCAATG	1 cut(s)	543
BseMII	CTCAG	1 cut(s)	404
BseNI	ACTGG	3 cut(s)	135, 767, 824
BseRI	GAGGAG	7 cut(s)	109, 316, 325, 328, 340, 365, 979
BseX3I	CGGCCG	1 cut(s)	174
BseXI	GCAGC	8 cut(s)	27, 157, 179, 187, 211, 239, 925, 1036
BsgI	GTGCAG	1 cut(s)	74
Bsh1285I	CGRYCG	1 cut(s)	177
BshFI	GGCC	5 cut(s)	5, 176, 194, 212, 1041
BshNI	GGYRCC	1 cut(s)	131
BsiEI	CGRYCG	1 cut(s)	177
BsiHKAI	GWGCWC	1 cut(s)	419
BsiHKCI	CYCGRG	1 cut(s)	195
BsiSI	CCGG	2 cut(s)	196, 616
BslFI	GGGAC	1 cut(s)	633
BslI	CCNNNNNNNGG	1 cut(s)	682
BsmAI	GTCTC	3 cut(s)	148, 452, 634
BsmFI	GGGAC	1 cut(s)	633
BsmI	GAATGC	1 cut(s)	440
BsnI	GGCC	5 cut(s)	5, 176, 194, 212, 1041
Bso31I	GGTCTC	1 cut(s)	148
BsoBI	CYCGRG	1 cut(s)	195
Bsp1286I	GDGCHC	2 cut(s)	419, 793
Bsp143I	GATC	4 cut(s)	724, 768, 988, 1028
BspACI	CCGC	5 cut(s)	173, 213, 230, 837, 974
BspANI	GGCC	5 cut(s)	5, 176, 194, 212, 1041
BspCNI	CTCAG	1 cut(s)	405
BspHI	TCATGA	1 cut(s)	727
BspLI	GGNNCC	1 cut(s)	133
BspMAI	CTGCAG	2 cut(s)	229, 1026
BspPI	GGATC	2 cut(s)	776, 996
BspQI	GCTCTTC	1 cut(s)	495
BspT107I	GGYRCC	1 cut(s)	131
BspTNI	GGTCTC	1 cut(s)	148
BsrDI	GCAATG	1 cut(s)	543
BsrI	ACTGG	3 cut(s)	135, 767, 824
BssECI	CCNNGG	4 cut(s)	177, 195, 531, 616
BssMI	GATC	4 cut(s)	724, 768, 988, 1028
BssNI	GRCGYC	1 cut(s)	132
BssT1I	CCWWGG	1 cut(s)	531
Bst4CI	ACNGT	2 cut(s)	946, 1003
Bst6I	CTCTTC	4 cut(s)	112, 118, 302, 495
BstACI	GRCGYC	1 cut(s)	132
BstC8I	GCNNGC	5 cut(s)	186, 192, 640, 777, 936
BstDEI	CTNAG	2 cut(s)	413, 443
BstENI	CCTNNNNNAGG	1 cut(s)	680
BstF5I	GGATG	3 cut(s)	244, 352, 743
BstH2I	RGCGCY	3 cut(s)	135, 185, 191
BstHHI	GCGC	3 cut(s)	134, 184, 190
BstKTI	GATC	4 cut(s)	727, 771, 991, 1031
BstMAI	GTCTC	3 cut(s)	148, 452, 634
BstMBI	GATC	4 cut(s)	724, 768, 988, 1028
BstMCI	CGRYCG	1 cut(s)	177
BstMWI	GCNNNNNNNGC	6 cut(s)	173, 179, 200, 209, 221, 230
BstNSI	RCATGY	2 cut(s)	938, 1018
BstSCI	CCNGG	3 cut(s)	194, 195, 615
BstSFI	CTRYAG	3 cut(s)	204, 225, 1022
BstV1I	GCAGC	8 cut(s)	27, 157, 179, 187, 211, 239, 925, 1036
BstV2I	GAAGAC	1 cut(s)	554
BstXI	CCANNNNNNTGG	1 cut(s)	538
BstZI	CGGCCG	1 cut(s)	174
BsuRI	GGCC	5 cut(s)	5, 176, 194, 212, 1041
BtsCI	GGATG	3 cut(s)	244, 352, 743
BtsI	GCAGTG	1 cut(s)	1019
BtsIMutI	CAGTG	1 cut(s)	1019
Cac8I	GCNNGC	5 cut(s)	186, 192, 640, 777, 936
CaiI	CAGNNNCTG	2 cut(s)	511, 895
CciI	TCATGA	1 cut(s)	727
CfoI	GCGC	3 cut(s)	134, 184, 190
Cfr13I	GGNCC	2 cut(s)	193, 1039
Cfr9I	CCCGGG	1 cut(s)	195
CviAII	CATG	4 cut(s)	728, 935, 1015, 1032
DdeI	CTNAG	2 cut(s)	413, 443
DinI	GGCGCC	1 cut(s)	133
DpnI	GATC	4 cut(s)	726, 770, 990, 1030
DpnII	GATC	4 cut(s)	724, 768, 988, 1028
DriI	GACNNNNNGTC	2 cut(s)	626, 802
EaeI	YGGCCR	2 cut(s)	3, 174
EagI	CGGCCG	1 cut(s)	174
Eam1104I	CTCTTC	4 cut(s)	112, 118, 302, 495
Eam1105I	GACNNNNNGTC	2 cut(s)	626, 802
EarI	CTCTTC	4 cut(s)	112, 118, 302, 495
Ecl136II	GAGCTC	1 cut(s)	417
EclXI	CGGCCG	1 cut(s)	174
Eco130I	CCWWGG	1 cut(s)	531
Eco24I	GRGCYC	2 cut(s)	419, 793
Eco31I	GGTCTC	1 cut(s)	148
Eco52I	CGGCCG	1 cut(s)	174
Eco53kI	GAGCTC	1 cut(s)	417
Eco88I	CYCGRG	1 cut(s)	195
EcoICRI	GAGCTC	1 cut(s)	417
EcoNI	CCTNNNNNAGG	1 cut(s)	680
EcoT14I	CCWWGG	1 cut(s)	531
EcoT38I	GRGCYC	2 cut(s)	419, 793
EgeI	GGCGCC	1 cut(s)	133
EheI	GGCGCC	1 cut(s)	133
ErhI	CCWWGG	1 cut(s)	531
FaeI	CATG	4 cut(s)	731, 938, 1018, 1035
FaqI	GGGAC	1 cut(s)	633
FatI	CATG	4 cut(s)	727, 934, 1014, 1031
FauNDI	CATATG	2 cut(s)	20, 904
FbaI	TGATCA	1 cut(s)	724
FblI	GTMKAC	1 cut(s)	759
FokI	GGATG	3 cut(s)	251, 359, 750
FriOI	GRGCYC	2 cut(s)	419, 793
FspBI	CTAG	3 cut(s)	467, 794, 807
GlaI	GCGC	3 cut(s)	133, 183, 189
HaeII	RGCGCY	3 cut(s)	135, 185, 191
HaeIII	GGCC	5 cut(s)	5, 176, 194, 212, 1041
HapII	CCGG	2 cut(s)	196, 616
HhaI	GCGC	3 cut(s)	134, 184, 190
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	4 cut(s)	731, 938, 1018, 1035
Hin6I	GCGC	3 cut(s)	132, 182, 188
HinP1I	GCGC	3 cut(s)	132, 182, 188
HindIII	AAGCTT	2 cut(s)	664, 950
HinfI	GANTC	5 cut(s)	470, 486, 587, 600, 621
HpaII	CCGG	2 cut(s)	196, 616
HphI	GGTGA	2 cut(s)	21, 307
Hpy166II	GTNNAC	2 cut(s)	760, 829
Hpy188I	TCNGA	3 cut(s)	414, 788, 818
Hpy188III	TCNNGA	6 cut(s)	474, 607, 671, 683, 728, 807
Hpy8I	GTNNAC	2 cut(s)	760, 829
HpyAV	CCTTC	3 cut(s)	244, 680, 883
HpyCH4III	ACNGT	2 cut(s)	946, 1003
HpyCH4IV	ACGT	2 cut(s)	756, 831
HpyCH4V	TGCA	8 cut(s)	91, 167, 227, 934, 941, 1014, 1024, 1035
HpyF10VI	GCNNNNNNNGC	6 cut(s)	173, 179, 200, 209, 221, 230
HpyF3I	CTNAG	2 cut(s)	413, 443
HpySE526I	ACGT	2 cut(s)	756, 831
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	4 cut(s)	731, 938, 1018, 1035
HspAI	GCGC	3 cut(s)	132, 182, 188
KasI	GGCGCC	1 cut(s)	131
Ksp22I	TGATCA	1 cut(s)	724
Kzo9I	GATC	4 cut(s)	724, 768, 988, 1028
LguI	GCTCTTC	1 cut(s)	495
LmnI	GCTCC	5 cut(s)	458, 508, 609, 690, 966
Lsp1109I	GCAGC	8 cut(s)	27, 157, 179, 187, 211, 239, 925, 1036
LweI	GCATC	2 cut(s)	211, 418
MaeI	CTAG	3 cut(s)	467, 794, 807
MaeII	ACGT	2 cut(s)	756, 831
MaeIII	GTNAC	3 cut(s)	588, 997, 1017
MalI	GATC	4 cut(s)	726, 770, 990, 1030
MboI	GATC	4 cut(s)	724, 768, 988, 1028
MboII	GAAGA	7 cut(s)	99, 105, 319, 437, 489, 512, 559
MhlI	GDGCHC	2 cut(s)	419, 793
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	4 cut(s)	125, 868, 912, 978
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	132
MlyI	GAGTC	3 cut(s)	495, 596, 615
MmeI	TCCRAC	4 cut(s)	68, 109, 315, 349
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	482, 662
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	170, 230
MspI	CCGG	2 cut(s)	196, 616
MspR9I	CCNGG	3 cut(s)	196, 197, 617
Mva1269I	GAATGC	1 cut(s)	440
MwoI	GCNNNNNNNGC	6 cut(s)	173, 179, 200, 209, 221, 230
NarI	GGCGCC	1 cut(s)	132
NciI	CCSGG	3 cut(s)	196, 197, 617
NdeI	CATATG	2 cut(s)	20, 904
NdeII	GATC	4 cut(s)	724, 768, 988, 1028
NlaIII	CATG	4 cut(s)	731, 938, 1018, 1035
NlaIV	GGNNCC	1 cut(s)	133
NmeAIII	GCCGAG	1 cut(s)	202
NmuCI	GTSAC	2 cut(s)	588, 1017
NspI	RCATGY	2 cut(s)	938, 1018
PaeI	GCATGC	1 cut(s)	938
PagI	TCATGA	1 cut(s)	727
PciSI	GCTCTTC	1 cut(s)	495
PctI	GAATGC	1 cut(s)	440
PfeI	GAWTC	2 cut(s)	470, 600
PleI	GAGTC	3 cut(s)	494, 595, 615
PluTI	GGCGCC	1 cut(s)	135
PpsI	GAGTC	3 cut(s)	494, 595, 615
Psp124BI	GAGCTC	1 cut(s)	419
PspN4I	GGNNCC	1 cut(s)	133
PspPI	GGNCC	2 cut(s)	193, 1039
PstI	CTGCAG	2 cut(s)	229, 1026
PstNI	CAGNNNCTG	2 cut(s)	511, 895
PvuII	CAGCTG	1 cut(s)	170
SacI	GAGCTC	1 cut(s)	419
SapI	GCTCTTC	1 cut(s)	495
SaqAI	TTAA	2 cut(s)	482, 662
Sau3AI	GATC	4 cut(s)	724, 768, 988, 1028
Sau96I	GGNCC	2 cut(s)	193, 1039
SchI	GAGTC	3 cut(s)	495, 596, 615
ScrFI	CCNGG	3 cut(s)	196, 197, 617
SduI	GDGCHC	2 cut(s)	419, 793
SfaNI	GCATC	2 cut(s)	211, 418
SfcI	CTRYAG	3 cut(s)	204, 225, 1022
SfoI	GGCGCC	1 cut(s)	133
SmaI	CCCGGG	1 cut(s)	197
SmlI	CTYRAG	1 cut(s)	449
SmoI	CTYRAG	1 cut(s)	449
SphI	GCATGC	1 cut(s)	938
SrfI	GCCCGGGC	1 cut(s)	197
Sse9I	AATT	4 cut(s)	125, 868, 912, 978
SsiI	CCGC	5 cut(s)	173, 213, 230, 837, 974
SspDI	GGCGCC	1 cut(s)	131
SspMI	CTAG	3 cut(s)	467, 794, 807
SstI	GAGCTC	1 cut(s)	419
StyD4I	CCNGG	3 cut(s)	194, 195, 615
StyI	CCWWGG	1 cut(s)	531
TaaI	ACNGT	2 cut(s)	946, 1003
TaiI	ACGT	2 cut(s)	759, 834
TaqI	TCGA	2 cut(s)	706, 991
TasI	AATT	4 cut(s)	125, 868, 912, 978
TauI	GCSGC	3 cut(s)	176, 215, 233
TfiI	GAWTC	2 cut(s)	470, 600
Tru1I	TTAA	2 cut(s)	482, 662
Tru9I	TTAA	2 cut(s)	482, 662
TscAI	CASTG	1 cut(s)	1026
TseFI	GTSAC	2 cut(s)	588, 1017
TseI	GCWGC	8 cut(s)	15, 167, 170, 200, 224, 227, 938, 1024
Tsp45I	GTSAC	2 cut(s)	588, 1017
TspDTI	ATGAA	5 cut(s)	15, 560, 744, 924, 972
TspMI	CCCGGG	1 cut(s)	195
TspRI	CASTG	1 cut(s)	1026
XagI	CCTNNNNNAGG	1 cut(s)	680
XapI	RAATTY	2 cut(s)	125, 868
XbaI	TCTAGA	1 cut(s)	806
XceI	RCATGY	2 cut(s)	938, 1018
XcmI	CCANNNNNNNNNTGG	1 cut(s)	154
XmaI	CCCGGG	1 cut(s)	195
XmiI	GTMKAC	1 cut(s)	759
XspI	CTAG	3 cut(s)	467, 794, 807

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.