Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005329

Physical Location & Seq

Genomic Coordinates Reverse (-)

1839567 .. 1842555

Gene Structure 2989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1021800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGGCCATTACCGGCAGCATATGTTCATCACCAAACCAACATCCAACTCTCCCAACACTTCCACTCCCACCAATCCCACCACTCAAAACCCACCACCCTCTCCAACTCCACTTCCCTCCTCCCTCTTTCTCCAAATTTGGCGCCAACTGGGTCTCCAGTGACGCATTGGTCATTGCAGCCACGGCGGAGGCGCTGGCACTGGCCCAGGCTTCCGTGAAGGTGGCCAGAGATGCTGCGGCGGTGAGGGATGAGATTGTGGAGGCTTGGAGTTGTAGGGAGAGTGGGAATGGGAGTGGTGGGTTGGCGGTGAGGAGGAAGAGGAGGAGGAAAAGGATAAAAGGGTTGGAGGGTTTGGATGAGGAGAGGGGAAGGGGTGTTGGAGATGGAAAGTTGTCGTTTGGTTATGTGAGATGTGGGCATCTGAGCCCAAACGAAGAAGATGAATTCTGTCTCAGTCTCAAGGAGGGAGCAAGATTAGAATTGCGAAGATTAAGGGTCGTAGAAGCTGAAGAGCAGGAGCCGACCTCAAAGCAGTTAGCCAAGGCAATGGGAATGAAGACGAGAAGTATAGATAAGGTGCTTTGTAAGAAAAGAGAGTCACAAGAAAGGATCATCAGGAGCTACCGGGGACTCATTATCTCTGTTGCCAGCAGTTATCAAGGCAAAGGACTAAGCTTTCAAGACCTCATTCAGGAAGGAAGCGACGGGCTTGTTCAAGTGGCAGAGAAGTTTGATCATGAGCGAGGATGGAAGTTATCAACATACGTCTACTGGTGGATCAGGCAAGCTATTATCAGAGCCCTAGACAACAAGTCTAGAATAATCAGATTACCAGAGATCATGCAAGGGCCAGAGGAGATGATGCCGGAAAAGATGCTTACAAAACAGCTAATGAAGCAGGACGAGAAGAAGCTTCTGAAGACGCTGAGCGATGAAGCATATGTATTGAGATTACACTTTGGACTCAATGGTGAGACCCCTAAGTCCTTTGAGGAGATAGGAAGATTGTTAAAGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

340

Amino Acids

38.09

Weight (kDa)

9.4

Isoelectric Point (pI)

47.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	204 - 273	4.7e-17	Sigma-70 region 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13730
fragaria_vesca	FvH4_7g01630
malus_domestica	MD02G1302300.v1.1 MD07G1021800.v1.1
prunus_persica	Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1 Prupe.2G021900_v2.0.a1
pyrus_communis	pycom02g25460 pycom07g01650
rosa_chinensis	RchiOBHm_Chr1g0317901
rosa_laevigata	RLG00000029590 RLG00000030578
rosa_multiflora	Rmu_sc0000654.1_g000054 Rmu_ssc0000008.1_g000049
rosa_roxburghii	Rroxscaffold_4G00330140
rosa_rugosa	Rorug01G0018500
rosa_samantha	Rh1AG030000 Rh1BG026000 Rh1BG026100 Rh1CG028600 Rh1DG041500
rosa_wichuraiana	Rw1G002260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	167, 982
AccB1I	GGYRCC	1 cut(s)	140
AccI	GTMKAC	1 cut(s)	768
AciI	CCGC	4 cut(s)	185, 236, 239, 305
AclWI	GGATC	2 cut(s)	617, 785
AcoI	YGGCCR	2 cut(s)	3, 222
AcsI	RAATTY	2 cut(s)	134, 443
AcuI	CTGAAG	2 cut(s)	528, 938
AcyI	GRCGYC	1 cut(s)	141
AfiI	CCNNNNNNNGG	1 cut(s)	691
AgsI	TTSAA	2 cut(s)	680, 716
AhdI	GACNNNNNGTC	1 cut(s)	811
AjnI	CCWGG	1 cut(s)	204
AluBI	AGCT	6 cut(s)	506, 621, 675, 788, 889, 913
AluI	AGCT	6 cut(s)	506, 621, 675, 788, 889, 913
Alw26I	GTCTC	4 cut(s)	157, 455, 461, 968
AlwI	GGATC	2 cut(s)	617, 785
AoxI	GGCC	4 cut(s)	3, 201, 222, 848
ApeKI	GCWGC	3 cut(s)	15, 176, 233
ApoI	RAATTY	2 cut(s)	134, 443
AspLEI	GCGC	2 cut(s)	143, 193
AspS9I	GGNCC	2 cut(s)	202, 848
AsuC2I	CCSGG	1 cut(s)	626
AsuHPI	GGTGA	4 cut(s)	21, 253, 319, 983
BalI	TGGCCA	2 cut(s)	5, 224
BanI	GGYRCC	1 cut(s)	140
BanII	GRGCYC	2 cut(s)	428, 802
BbsI	GAAGAC	2 cut(s)	563, 926
BbvI	GCAGC	3 cut(s)	27, 188, 220
BccI	CCATC	2 cut(s)	377, 741
BceAI	ACGGC	1 cut(s)	198
BciT130I	CCWGG	1 cut(s)	206
BclI	TGATCA	1 cut(s)	733
BcnI	CCSGG	1 cut(s)	626
BcoDI	GTCTC	4 cut(s)	157, 455, 461, 968
BfaI	CTAG	2 cut(s)	803, 816
BfoI	RGCGCY	2 cut(s)	144, 194
BisI	GCNGC	4 cut(s)	16, 177, 234, 237
BlpI	GCTNAGC	1 cut(s)	926
BlsI	GCNGC	4 cut(s)	17, 178, 235, 238
Bme1390I	CCNGG	2 cut(s)	206, 626
BmeRI	GACNNNNNGTC	1 cut(s)	811
BmgT120I	GGNCC	2 cut(s)	202, 848
BmiI	GGNNCC	2 cut(s)	142, 519
BmrFI	CCNGG	2 cut(s)	206, 626
BmrI	ACTGGG	1 cut(s)	157
BmsI	GCATC	4 cut(s)	220, 427, 852, 864
BmuI	ACTGGG	1 cut(s)	157
BpiI	GAAGAC	2 cut(s)	563, 926
BplI	GAGNNNNNCTC	2 cut(s)	509, 541
BpmI	CTGGAG	1 cut(s)	139
Bpu1102I	GCTNAGC	1 cut(s)	926
BpuEI	CTTGAG	1 cut(s)	443
BpuMI	CCSGG	1 cut(s)	626
BsaHI	GRCGYC	1 cut(s)	141
BsaI	GGTCTC	2 cut(s)	157, 968
BsaJI	CCNNGG	4 cut(s)	180, 204, 540, 625
BsaXI	ACNNNNNCTCC	4 cut(s)	48, 78, 137, 167
Bsc4I	CCNNNNNNNGG	1 cut(s)	691
Bse118I	RCCGGY	1 cut(s)	11
Bse1I	ACTGG	4 cut(s)	152, 156, 204, 776
Bse3DI	GCAATG	2 cut(s)	171, 552
BseBI	CCWGG	1 cut(s)	206
BseDI	CCNNGG	4 cut(s)	180, 204, 540, 625
BseGI	GGATG	4 cut(s)	40, 253, 361, 752
BseLI	CCNNNNNNNGG	1 cut(s)	691
BseMI	GCAATG	2 cut(s)	171, 552
BseMII	CTCAG	3 cut(s)	413, 466, 917
BseNI	ACTGG	4 cut(s)	152, 156, 204, 776
BseRI	GAGGAG	7 cut(s)	108, 325, 334, 337, 374, 869, 1007
BseXI	GCAGC	3 cut(s)	27, 188, 220
BshFI	GGCC	4 cut(s)	5, 203, 224, 850
BshNI	GGYRCC	1 cut(s)	140
BsiSI	CCGG	3 cut(s)	12, 625, 866
BslFI	GGGAC	1 cut(s)	642
BslI	CCNNNNNNNGG	1 cut(s)	691
BsmAI	GTCTC	4 cut(s)	157, 455, 461, 968
BsmFI	GGGAC	1 cut(s)	642
BsnI	GGCC	4 cut(s)	5, 203, 224, 850
Bso31I	GGTCTC	2 cut(s)	157, 968
Bsp1286I	GDGCHC	2 cut(s)	428, 802
Bsp143I	GATC	4 cut(s)	609, 733, 777, 837
Bsp1720I	GCTNAGC	1 cut(s)	926
BspACI	CCGC	4 cut(s)	185, 236, 239, 305
BspANI	GGCC	4 cut(s)	5, 203, 224, 850
BspCNI	CTCAG	3 cut(s)	414, 465, 918
BspHI	TCATGA	1 cut(s)	736
BspLI	GGNNCC	2 cut(s)	142, 519
BspPI	GGATC	2 cut(s)	617, 785
BspQI	GCTCTTC	1 cut(s)	504
BspT107I	GGYRCC	1 cut(s)	140
BspTNI	GGTCTC	2 cut(s)	157, 968
BsrDI	GCAATG	2 cut(s)	171, 552
BsrFI	RCCGGY	1 cut(s)	11
BsrI	ACTGG	4 cut(s)	152, 156, 204, 776
BssAI	RCCGGY	1 cut(s)	11
BssECI	CCNNGG	4 cut(s)	180, 204, 540, 625
BssMI	GATC	4 cut(s)	609, 733, 777, 837
BssNI	GRCGYC	1 cut(s)	141
BssT1I	CCWWGG	1 cut(s)	540
Bst2UI	CCWGG	1 cut(s)	206
Bst6I	CTCTTC	2 cut(s)	311, 504
BstACI	GRCGYC	1 cut(s)	141
BstC8I	GCNNGC	3 cut(s)	195, 649, 786
BstDEI	CTNAG	5 cut(s)	422, 452, 671, 926, 981
BstDSI	CCRYGG	1 cut(s)	180
BstENI	CCTNNNNNAGG	1 cut(s)	689
BstF5I	GGATG	4 cut(s)	40, 253, 361, 752
BstH2I	RGCGCY	2 cut(s)	144, 194
BstHHI	GCGC	2 cut(s)	143, 193
BstKTI	GATC	4 cut(s)	612, 736, 780, 840
BstMAI	GTCTC	4 cut(s)	157, 455, 461, 968
BstMBI	GATC	4 cut(s)	609, 733, 777, 837
BstMWI	GCNNNNNNNGC	3 cut(s)	182, 230, 895
BstNI	CCWGG	1 cut(s)	206
BstSCI	CCNGG	2 cut(s)	204, 624
BstV1I	GCAGC	3 cut(s)	27, 188, 220
BstV2I	GAAGAC	2 cut(s)	563, 926
BstXI	CCANNNNNNTGG	1 cut(s)	547
BsuRI	GGCC	4 cut(s)	5, 203, 224, 850
BtgI	CCRYGG	1 cut(s)	180
BtgZI	GCGATG	1 cut(s)	945
BtsCI	GGATG	4 cut(s)	40, 253, 361, 752
BtsIMutI	CAGTG	2 cut(s)	163, 197
Cac8I	GCNNGC	3 cut(s)	195, 649, 786
CciI	TCATGA	1 cut(s)	736
CfoI	GCGC	2 cut(s)	143, 193
Cfr10I	RCCGGY	1 cut(s)	11
Cfr13I	GGNCC	2 cut(s)	202, 848
CseI	GACGC	2 cut(s)	170, 931
CviAII	CATG	2 cut(s)	737, 841
DdeI	CTNAG	5 cut(s)	422, 452, 671, 926, 981
DinI	GGCGCC	1 cut(s)	142
DpnI	GATC	4 cut(s)	611, 735, 779, 839
DpnII	GATC	4 cut(s)	609, 733, 777, 837
DrdI	GACNNNNNNGTC	2 cut(s)	167, 982
DriI	GACNNNNNGTC	1 cut(s)	811
DseDI	GACNNNNNNGTC	2 cut(s)	167, 982
EaeI	YGGCCR	2 cut(s)	3, 222
Eam1104I	CTCTTC	2 cut(s)	311, 504
Eam1105I	GACNNNNNGTC	1 cut(s)	811
EarI	CTCTTC	2 cut(s)	311, 504
EciI	GGCGGA	1 cut(s)	200
Eco130I	CCWWGG	1 cut(s)	540
Eco24I	GRGCYC	2 cut(s)	428, 802
Eco31I	GGTCTC	2 cut(s)	157, 968
Eco57I	CTGAAG	2 cut(s)	528, 938
EcoNI	CCTNNNNNAGG	1 cut(s)	689
EcoRI	GAATTC	1 cut(s)	443
EcoRII	CCWGG	1 cut(s)	204
EcoT14I	CCWWGG	1 cut(s)	540
EcoT38I	GRGCYC	2 cut(s)	428, 802
EgeI	GGCGCC	1 cut(s)	142
EheI	GGCGCC	1 cut(s)	142
ErhI	CCWWGG	1 cut(s)	540
FaeI	CATG	2 cut(s)	740, 844
FaiI	YATR	9 cut(s)	20, 22, 405, 569, 738, 763, 842, 940, 942
FaqI	GGGAC	1 cut(s)	642
FatI	CATG	2 cut(s)	736, 840
FauNDI	CATATG	2 cut(s)	20, 940
FbaI	TGATCA	1 cut(s)	733
FblI	GTMKAC	1 cut(s)	768
Fnu4HI	GCNGC	4 cut(s)	16, 177, 234, 237
FokI	GGATG	4 cut(s)	27, 260, 368, 759
FriOI	GRGCYC	2 cut(s)	428, 802
Fsp4HI	GCNGC	4 cut(s)	16, 177, 234, 237
FspBI	CTAG	2 cut(s)	803, 816
GlaI	GCGC	2 cut(s)	142, 192
GluI	GCNGC	4 cut(s)	16, 177, 234, 237
GsuI	CTGGAG	1 cut(s)	139
HaeII	RGCGCY	2 cut(s)	144, 194
HaeIII	GGCC	4 cut(s)	5, 203, 224, 850
HapII	CCGG	3 cut(s)	12, 625, 866
HgaI	GACGC	2 cut(s)	170, 931
HhaI	GCGC	2 cut(s)	143, 193
Hin1I	GRCGYC	1 cut(s)	141
Hin1II	CATG	2 cut(s)	740, 844
Hin6I	GCGC	2 cut(s)	141, 191
HinP1I	GCGC	2 cut(s)	141, 191
HindIII	AAGCTT	2 cut(s)	673, 911
HinfI	GANTC	3 cut(s)	596, 630, 963
HpaII	CCGG	3 cut(s)	12, 625, 866
HphI	GGTGA	4 cut(s)	21, 253, 319, 983
Hpy166II	GTNNAC	1 cut(s)	769
Hpy188I	TCNGA	4 cut(s)	423, 797, 827, 918
Hpy188III	TCNNGA	5 cut(s)	616, 680, 692, 737, 816
Hpy8I	GTNNAC	1 cut(s)	769
Hpy99I	CGWCG	1 cut(s)	707
HpyAV	CCTTC	3 cut(s)	211, 363, 689
HpyCH4IV	ACGT	1 cut(s)	765
HpyCH4V	TGCA	2 cut(s)	176, 844
HpyF10VI	GCNNNNNNNGC	3 cut(s)	182, 230, 895
HpyF3I	CTNAG	5 cut(s)	422, 452, 671, 926, 981
HpySE526I	ACGT	1 cut(s)	765
Hsp92I	GRCGYC	1 cut(s)	141
Hsp92II	CATG	2 cut(s)	740, 844
HspAI	GCGC	2 cut(s)	141, 191
KasI	GGCGCC	1 cut(s)	140
Ksp22I	TGATCA	1 cut(s)	733
Kzo9I	GATC	4 cut(s)	609, 733, 777, 837
LguI	GCTCTTC	1 cut(s)	504
LmnI	GCTCC	3 cut(s)	467, 517, 618
Lsp1109I	GCAGC	3 cut(s)	27, 188, 220
LweI	GCATC	4 cut(s)	220, 427, 852, 864
MaeI	CTAG	2 cut(s)	803, 816
MaeII	ACGT	1 cut(s)	765
MaeIII	GTNAC	2 cut(s)	158, 597
MalI	GATC	4 cut(s)	611, 735, 779, 839
MboI	GATC	4 cut(s)	609, 733, 777, 837
MboII	GAAGA	9 cut(s)	328, 446, 449, 498, 521, 568, 919, 931, 1014
MhlI	GDGCHC	2 cut(s)	428, 802
MlsI	TGGCCA	2 cut(s)	5, 224
MluCI	AATT	3 cut(s)	134, 443, 479
MluNI	TGGCCA	2 cut(s)	5, 224
Mly113I	GGCGCC	1 cut(s)	141
MlyI	GAGTC	3 cut(s)	605, 624, 957
MmeI	TCCRAC	4 cut(s)	68, 127, 324, 358
Mox20I	TGGCCA	2 cut(s)	5, 224
MscI	TGGCCA	2 cut(s)	5, 224
MseI	TTAA	3 cut(s)	491, 1010, 1018
Msp20I	TGGCCA	2 cut(s)	5, 224
MspI	CCGG	3 cut(s)	12, 625, 866
MspR9I	CCNGG	2 cut(s)	206, 626
MvaI	CCWGG	1 cut(s)	206
MwoI	GCNNNNNNNGC	3 cut(s)	182, 230, 895
NarI	GGCGCC	1 cut(s)	141
NciI	CCSGG	1 cut(s)	626
NdeI	CATATG	2 cut(s)	20, 940
NdeII	GATC	4 cut(s)	609, 733, 777, 837
NlaIII	CATG	2 cut(s)	740, 844
NlaIV	GGNNCC	2 cut(s)	142, 519
NmuCI	GTSAC	2 cut(s)	158, 597
PagI	TCATGA	1 cut(s)	736
PciSI	GCTCTTC	1 cut(s)	504
PkrI	GCNGC	4 cut(s)	17, 178, 235, 238
PleI	GAGTC	3 cut(s)	604, 624, 957
PluTI	GGCGCC	1 cut(s)	144
PpsI	GAGTC	3 cut(s)	604, 624, 957
Psp6I	CCWGG	1 cut(s)	204
PspGI	CCWGG	1 cut(s)	204
PspN4I	GGNNCC	2 cut(s)	142, 519
PspPI	GGNCC	2 cut(s)	202, 848
SapI	GCTCTTC	1 cut(s)	504
SaqAI	TTAA	3 cut(s)	491, 1010, 1018
SatI	GCNGC	4 cut(s)	16, 177, 234, 237
Sau3AI	GATC	4 cut(s)	609, 733, 777, 837
Sau96I	GGNCC	2 cut(s)	202, 848
SchI	GAGTC	3 cut(s)	605, 624, 957
ScrFI	CCNGG	2 cut(s)	206, 626
SduI	GDGCHC	2 cut(s)	428, 802
SfaNI	GCATC	4 cut(s)	220, 427, 852, 864
SfoI	GGCGCC	1 cut(s)	142
SmlI	CTYRAG	1 cut(s)	458
SmoI	CTYRAG	1 cut(s)	458
Sse9I	AATT	3 cut(s)	134, 443, 479
SsiI	CCGC	4 cut(s)	185, 236, 239, 305
SspDI	GGCGCC	1 cut(s)	140
SspMI	CTAG	2 cut(s)	803, 816
StyD4I	CCNGG	2 cut(s)	204, 624
StyI	CCWWGG	1 cut(s)	540
TaiI	ACGT	1 cut(s)	768
TasI	AATT	3 cut(s)	134, 443, 479
TauI	GCSGC	1 cut(s)	239
Tru1I	TTAA	3 cut(s)	491, 1010, 1018
Tru9I	TTAA	3 cut(s)	491, 1010, 1018
TscAI	CASTG	2 cut(s)	163, 204
TseFI	GTSAC	2 cut(s)	158, 597
TseI	GCWGC	3 cut(s)	15, 176, 233
Tsp45I	GTSAC	2 cut(s)	158, 597
TspDTI	ATGAA	5 cut(s)	15, 456, 569, 908, 948
TspGWI	ACGGA	1 cut(s)	202
TspRI	CASTG	2 cut(s)	163, 204
XagI	CCTNNNNNAGG	1 cut(s)	689
XapI	RAATTY	2 cut(s)	134, 443
XbaI	TCTAGA	1 cut(s)	815
XcmI	CCANNNNNNNNNTGG	1 cut(s)	163
XmiI	GTMKAC	1 cut(s)	768
XspI	CTAG	2 cut(s)	803, 816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.