MD02G1309700.v1.1

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008044
Physical Location & Seq
Forward (+)
36531630 .. 36536707
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1309700.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGTGGCTAGTTGTGCTTCATTACTCCCTAATTACTCTTATCTTCGTCGTGTTCTCCACTTTCTATTTGCTTGCTTGCTTTCTCGAGGTAGACTTTTCGGAGAACTGTTTCTCGGTGCATTTGGTGAAATATGCATCTGTTTTCCAGCATGTACGCATTGATTGTTTACGCACCTTCATTTCTGGTACTGCATTCAATGATTTATTTTACGGCTCAGCTATGGTTCATGGTATGCGCATCAAAGATGGAAAAGCAACTTATGTCTCCCGCTACGTAAGGACGTCACGTCTTAAGCAAGAAGAATATTTTGGAGGTTCTAAATTTATGAAGATTGGAGACCTTAAGGGGTTTCTCGGCTTATTCATGAAGAGCTTGCAAATGCTAAGAGAAAAACTGAAAATATTAGATATGTCTTATGGTTTTGGGACTGGTAACACAGCTCTCACATATCACCATGGGAAGCTTCTAGCTCTTCACGAGGCGGATAAACCTTATGTCCTCAAAGTTTTGGAAGATGGAGATCTGCAAACGGTTGGCTTGCTGGATTATGATAAGAGACTGACACATTCCTTCACTGCTCATCCAAAGGTTGACCCATTCACTGGTGAGATGTTTACATTTGGCTATTCATGGACTCCGCCATATGTCACTTACAGAGTTATTTCGAAGGATGGTTTCATGCATGACCCTATACCGATAACAATACCAGACCCTGTCATGATGCATGATTTTGCAATTACTGAGAACTATGCAATTTTCATGGATCTCCCCCTGATCTTCAAACCAAAGGAAATGGTTAAGGAACAAAAGTTGATATTCACATTTGATCAAACAAAAAAAGCTCGCTTTGGTTTACTTCCACGGTATGCAAAGGATGAGTTGCTAATCAGATGGTTCGAGCTTCCAAATTGCTTCATTTTCCATAATGCTAATGCTTGGGAGGAGGAGGATGAAGTTGTTTTAATCACATGCCGCTTTGAGAATTTTGATTTGGAAATGTTCAAAGAAAAGCTTGAAAACTTCAGAAATGAGCTGTACGAAATGAGATTCAACTTGAAAACTGGTCTAGCATCCCAAAAGAAACTATCAGAAGCGGCTGTAGATTTTCCCAGGGTGAATGAGAGCTACACCGGAAGGAAGCAGCGTTATGTGTACGGAACCTTGCTGGACAGCATTTTCAAGGTTACAGGAGTAGCCAAGTTTGATCTGCATGCTGAACCAGAGGTTGGAAAAACACAAATTGAGGTTGGAGGAAATGTCCAAGGCCTCTATGACCTGGGACCTGGCAGATTTGGTTCTGAGGCTATTTTTGTCCCTCGCACTCCTGGCGTTACTTCTGAAGAAGATGATGGCTACTTAATACTCTTTGTTCATGACGAGAACACTGGAAAATCAGCAATTCATGTAGTTGATGCAAAGACAATGTCACCAGACCCCGTTGCAGTTGTGGAATTGCCACACAGAGTTCCATACGGTTTGCATGCTATCTTCGTGACAGAGGAGCAATTGCAAGAACAAGCGAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

510

Amino Acids

58.48

Weight (kDa)

5.96

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 60 - 498 2e-120 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
Acc16I TGCGCA 1 cut(s) 236
AccB7I CCANNNNNTGG 2 cut(s) 602, 1226
AccI GTMKAC 1 cut(s) 90
AciI CCGC 5 cut(s) 268, 482, 638, 973, 1094
AclWI GGATC 1 cut(s) 771
AcsI RAATTY 2 cut(s) 320, 982
AcuI CTGAAG 2 cut(s) 1006, 1359
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 4 cut(s) 153, 187, 1037, 1154
AfiI CCNNNNNNNGG 2 cut(s) 602, 1226
AflII CTTAAG 2 cut(s) 290, 341
AgsI TTSAA 7 cut(s) 196, 781, 1003, 1016, 1051, 1057, 1180
AhdI GACNNNNNGTC 1 cut(s) 285
AjiI CACGTC 1 cut(s) 287
AjnI CCWGG 4 cut(s) 1109, 1275, 1282, 1324
AjuI GAANNNNNNNTTGG 6 cut(s) 291, 323, 974, 1006, 1209, 1241
Alw26I GTCTC 3 cut(s) 268, 330, 550
AlwI GGATC 1 cut(s) 771
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 1264
ApeKI GCWGC 1 cut(s) 1141
ApoI RAATTY 2 cut(s) 320, 982
ArsI GACNNNNNNTTYG 2 cut(s) 1409, 1441
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 1280
AsuHPI GGTGA 5 cut(s) 136, 443, 617, 1126, 1419
AsuII TTCGAA 1 cut(s) 665
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 1 cut(s) 1280
BauI CACGAG 1 cut(s) 476
BbvI GCAGC 1 cut(s) 1153
BccI CCATC 5 cut(s) 239, 509, 665, 885, 1343
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 4 cut(s) 1111, 1277, 1284, 1326
BclI TGATCA 1 cut(s) 826
BcoDI GTCTC 3 cut(s) 268, 330, 550
BfaI CTAG 3 cut(s) 8, 467, 1067
BfmI CTRYAG 1 cut(s) 1098
BfrI CTTAAG 2 cut(s) 290, 341
BglII AGATCT 1 cut(s) 520
BisI GCNGC 3 cut(s) 973, 1095, 1142
BlpI GCTNAGC 1 cut(s) 214
BlsI GCNGC 3 cut(s) 974, 1096, 1143
Bme1390I CCNGG 4 cut(s) 1111, 1277, 1284, 1326
Bme18I GGWCC 1 cut(s) 1280
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 83
BmgBI CACGTC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 2 cut(s) 1159, 1281
BmrFI CCNGG 4 cut(s) 1111, 1277, 1284, 1326
BmsI GCATC 5 cut(s) 143, 246, 711, 1079, 1402
Bpu1102I GCTNAGC 1 cut(s) 214
Bpu14I TTCGAA 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 274
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 6 cut(s) 454, 860, 1109, 1110, 1261, 1276
BsaWI WCCGGW 1 cut(s) 1130
BsaXI ACNNNNNCTCC 2 cut(s) 1242, 1272
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 1226
Bse1I ACTGG 4 cut(s) 433, 607, 1066, 1390
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 4 cut(s) 1111, 1277, 1284, 1326
BseDI CCNNGG 6 cut(s) 454, 860, 1109, 1110, 1261, 1276
BseGI GGATG 5 cut(s) 580, 676, 880, 955, 1070
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 2 cut(s) 602, 1226
BseMII CTCAG 3 cut(s) 228, 732, 1290
BseNI ACTGG 4 cut(s) 433, 607, 1066, 1390
BseRI GAGGAG 3 cut(s) 956, 959, 1514
BseXI GCAGC 1 cut(s) 1153
BshFI GGCC 1 cut(s) 1266
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 1 cut(s) 1131
BslFI GGGAC 3 cut(s) 439, 1293, 1298
BslI CCNNNNNNNGG 2 cut(s) 602, 1226
BsmAI GTCTC 3 cut(s) 268, 330, 550
BsmFI GGGAC 3 cut(s) 439, 1293, 1298
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 1 cut(s) 1266
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 665
Bsp143I GATC 5 cut(s) 520, 763, 774, 826, 1204
Bsp1720I GCTNAGC 1 cut(s) 214
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 5 cut(s) 268, 482, 638, 973, 1094
BspANI GGCC 1 cut(s) 1266
BspCNI CTCAG 3 cut(s) 227, 733, 1291
BspHI TCATGA 3 cut(s) 363, 717, 1372
BspLI GGNNCC 2 cut(s) 1159, 1281
BspPI GGATC 1 cut(s) 771
BspQI GCTCTTC 2 cut(s) 362, 477
BspT104I TTCGAA 1 cut(s) 665
BspTI CTTAAG 2 cut(s) 290, 341
BspTNI GGTCTC 1 cut(s) 330
BsrI ACTGG 4 cut(s) 433, 607, 1066, 1390
BssECI CCNNGG 6 cut(s) 454, 860, 1109, 1110, 1261, 1276
BssMI GATC 5 cut(s) 520, 763, 774, 826, 1204
BssNI GRCGYC 1 cut(s) 281
BssSI CACGAG 1 cut(s) 476
BssT1I CCWWGG 2 cut(s) 454, 1261
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 4 cut(s) 1111, 1277, 1284, 1326
Bst4CI ACNGT 4 cut(s) 107, 532, 864, 1475
Bst6I CTCTTC 2 cut(s) 362, 477
BstACI GRCGYC 1 cut(s) 281
BstAFI CTTAAG 2 cut(s) 290, 341
BstBAI YACGTR 1 cut(s) 274
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 7 cut(s) 72, 76, 374, 539, 844, 1212, 1482
BstDEI CTNAG 4 cut(s) 214, 383, 741, 1299
BstDSI CCRYGG 2 cut(s) 454, 860
BstF5I GGATG 5 cut(s) 580, 676, 880, 955, 1070
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 5 cut(s) 523, 766, 777, 829, 1207
BstMAI GTCTC 3 cut(s) 268, 330, 550
BstMBI GATC 5 cut(s) 520, 763, 774, 826, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 13, 1326
BstNI CCWGG 4 cut(s) 1111, 1277, 1284, 1326
BstNSI RCATGY 4 cut(s) 152, 972, 1214, 1484
BstSCI CCNGG 4 cut(s) 1109, 1275, 1282, 1324
BstSFI CTRYAG 1 cut(s) 1098
BstSNI TACGTA 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 1153
BstX2I RGATCY 2 cut(s) 520, 763
BstYI RGATCY 2 cut(s) 520, 763
BsuRI GGCC 1 cut(s) 1266
BtgI CCRYGG 2 cut(s) 454, 860
BtrI CACGTC 1 cut(s) 287
BtsCI GGATG 5 cut(s) 580, 676, 880, 955, 1070
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 3 cut(s) 573, 600, 1383
Cac8I GCNNGC 7 cut(s) 72, 76, 374, 539, 844, 1212, 1482
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 3 cut(s) 363, 717, 1372
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 1280
Csp6I GTAC 4 cut(s) 152, 186, 1036, 1153
CviQI GTAC 4 cut(s) 152, 186, 1036, 1153
DdeI CTNAG 4 cut(s) 214, 383, 741, 1299
DpnI GATC 5 cut(s) 522, 765, 776, 828, 1206
DpnII GATC 5 cut(s) 520, 763, 774, 826, 1204
DriI GACNNNNNGTC 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 362, 477
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 2 cut(s) 362, 477
EciI GGCGGA 2 cut(s) 497, 627
Eco105I TACGTA 1 cut(s) 274
Eco130I CCWWGG 2 cut(s) 454, 1261
Eco147I AGGCCT 1 cut(s) 1266
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 1280
Eco57I CTGAAG 2 cut(s) 1006, 1359
Eco88I CYCGRG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRII CCWGG 4 cut(s) 1109, 1275, 1282, 1324
EcoT14I CCWWGG 2 cut(s) 454, 1261
EcoT22I ATGCAT 3 cut(s) 136, 684, 726
ErhI CCWWGG 2 cut(s) 454, 1261
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 3 cut(s) 439, 1293, 1298
FauI CCCGC 1 cut(s) 275
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 973, 1095, 1142
FokI GGATG 5 cut(s) 567, 683, 887, 962, 1057
Fsp4HI GCNGC 3 cut(s) 973, 1095, 1142
FspAI RTGCGCAY 1 cut(s) 236
FspBI CTAG 3 cut(s) 8, 467, 1067
FspI TGCGCA 1 cut(s) 236
GlaI GCGC 1 cut(s) 236
GluI GCNGC 3 cut(s) 973, 1095, 1142
HaeIII GGCC 1 cut(s) 1266
HapII CCGG 1 cut(s) 1131
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HindIII AAGCTT 2 cut(s) 461, 1010
HinfI GANTC 2 cut(s) 634, 1047
HpaII CCGG 1 cut(s) 1131
HphI GGTGA 5 cut(s) 136, 443, 617, 1126, 1419
Hpy166II GTNNAC 6 cut(s) 91, 167, 592, 615, 854, 1153
Hpy188I TCNGA 6 cut(s) 100, 890, 1025, 1090, 1300, 1339
Hpy188III TCNNGA 6 cut(s) 83, 364, 476, 718, 1373, 1492
Hpy8I GTNNAC 6 cut(s) 91, 167, 592, 615, 854, 1153
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 184, 580, 661, 1128
HpyCH4III ACNGT 4 cut(s) 107, 532, 864, 1475
HpyCH4IV ACGT 3 cut(s) 273, 281, 286
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 1326
HpyF3I CTNAG 4 cut(s) 214, 383, 741, 1299
HpySE526I ACGT 3 cut(s) 273, 281, 286
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 5 cut(s) 520, 763, 774, 826, 1204
LguI GCTCTTC 2 cut(s) 362, 477
LmnI GCTCC 1 cut(s) 1501
Lsp1109I GCAGC 1 cut(s) 1153
LweI GCATC 5 cut(s) 143, 246, 711, 1079, 1402
MaeI CTAG 3 cut(s) 8, 467, 1067
MaeII ACGT 3 cut(s) 273, 281, 286
MaeIII GTNAC 7 cut(s) 282, 431, 646, 1183, 1330, 1425, 1492
MalI GATC 5 cut(s) 522, 765, 776, 828, 1206
MboI GATC 5 cut(s) 520, 763, 774, 826, 1204
MfeI CAATTG 1 cut(s) 1505
MflI RGATCY 2 cut(s) 520, 763
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 2 cut(s) 1207, 1228
Mph1103I ATGCAT 3 cut(s) 136, 684, 726
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 5 cut(s) 291, 342, 798, 962, 1358
MspCI CTTAAG 2 cut(s) 290, 341
MspI CCGG 1 cut(s) 1131
MspR9I CCNGG 4 cut(s) 1111, 1277, 1284, 1326
MunI CAATTG 1 cut(s) 1505
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 4 cut(s) 1111, 1277, 1284, 1326
MwoI GCNNNNNNNGC 2 cut(s) 13, 1326
NcoI CCATGG 1 cut(s) 454
NdeI CATATG 1 cut(s) 643
NdeII GATC 5 cut(s) 520, 763, 774, 826, 1204
NlaIV GGNNCC 2 cut(s) 1159, 1281
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 4 cut(s) 282, 646, 1425, 1492
NsbI TGCGCA 1 cut(s) 236
NsiI ATGCAT 3 cut(s) 136, 684, 726
NspI RCATGY 4 cut(s) 152, 972, 1214, 1484
NspV TTCGAA 1 cut(s) 665
PaeI GCATGC 2 cut(s) 1214, 1484
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 3 cut(s) 363, 717, 1372
PasI CCCWGGG 1 cut(s) 1110
PceI AGGCCT 1 cut(s) 1266
PciSI GCTCTTC 2 cut(s) 362, 477
PctI GAATGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 1047
PflFI GACNNNGTC 2 cut(s) 713, 1423
PflMI CCANNNNNTGG 2 cut(s) 602, 1226
PkrI GCNGC 3 cut(s) 974, 1096, 1143
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 274
PpuMI RGGWCCY 1 cut(s) 1280
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 4 cut(s) 1109, 1275, 1282, 1324
PspGI CCWGG 4 cut(s) 1109, 1275, 1282, 1324
PspN4I GGNNCC 2 cut(s) 1159, 1281
PspPI GGNCC 1 cut(s) 1280
PspPPI RGGWCCY 1 cut(s) 1280
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 2 cut(s) 520, 763
PsyI GACNNNGTC 2 cut(s) 713, 1423
RsaI GTAC 4 cut(s) 153, 187, 1037, 1154
RsaNI GTAC 4 cut(s) 152, 186, 1036, 1153
SapI GCTCTTC 2 cut(s) 362, 477
SaqAI TTAA 5 cut(s) 291, 342, 798, 962, 1358
SatI GCNGC 3 cut(s) 973, 1095, 1142
Sau3AI GATC 5 cut(s) 520, 763, 774, 826, 1204
Sau96I GGNCC 1 cut(s) 1280
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 4 cut(s) 1111, 1277, 1284, 1326
SfaNI GCATC 5 cut(s) 143, 246, 711, 1079, 1402
SfcI CTRYAG 1 cut(s) 1098
Sfr274I CTCGAG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 665
SinI GGWCC 1 cut(s) 1280
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 83, 290, 341
SmoI CTYRAG 3 cut(s) 83, 290, 341
SnaBI TACGTA 1 cut(s) 274
SphI GCATGC 2 cut(s) 1214, 1484
SseBI AGGCCT 1 cut(s) 1266
SsiI CCGC 5 cut(s) 268, 482, 638, 973, 1094
SspI AATATT 2 cut(s) 305, 402
SspMI CTAG 3 cut(s) 8, 467, 1067
StuI AGGCCT 1 cut(s) 1266
StyD4I CCNGG 4 cut(s) 1109, 1275, 1282, 1324
StyI CCWWGG 2 cut(s) 454, 1261
TaaI ACNGT 4 cut(s) 107, 532, 864, 1475
TaiI ACGT 3 cut(s) 276, 284, 289
TaqI TCGA 3 cut(s) 84, 665, 897
TauI GCSGC 2 cut(s) 975, 1097
TfiI GAWTC 1 cut(s) 1047
Tru1I TTAA 5 cut(s) 291, 342, 798, 962, 1358
Tru9I TTAA 5 cut(s) 291, 342, 798, 962, 1358
TscAI CASTG 3 cut(s) 580, 607, 1390
TseFI GTSAC 4 cut(s) 282, 646, 1425, 1492
TseI GCWGC 1 cut(s) 1141
Tsp45I GTSAC 4 cut(s) 282, 646, 1425, 1492
TspGWI ACGGA 1 cut(s) 1170
TspRI CASTG 3 cut(s) 580, 607, 1390
Tth111I GACNNNGTC 2 cut(s) 713, 1423
Van91I CCANNNNNTGG 2 cut(s) 602, 1226
Vha464I CTTAAG 2 cut(s) 290, 341
VpaK11BI GGWCC 1 cut(s) 1280
XapI RAATTY 2 cut(s) 320, 982
XceI RCATGY 4 cut(s) 152, 972, 1214, 1484
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 3 cut(s) 8, 467, 1067
ZraI GACGTC 1 cut(s) 282
Zsp2I ATGCAT 3 cut(s) 136, 684, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.