RLG00000030689

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008044
Physical Location & Seq
Forward (+)
65962383 .. 65970811
8429 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030689

Sequence Viewer

Length: 1656 bp
ATGGCGGAGGTGGTCGAGAAACCTCACGATGATGTGAAGGAAAAGCTGAAGAGCAACGGCATAGTGGTGCCGAATCCCAAGCCGAGCAAAGGCGTCACCTCCAAACTGGTGGACATGGTTGAGAAGCTCATTGTCAAGTTGATGTACGACAGTTCTCAGCCTCACCACTATCTCGCCGGAAACTTTGCTCCGGTTATTGAGGAAACGCCTCCCACTAAGGACCTCAATGTCATCGGCCATCTTCCTGATTGCTTGAATGGAGAGTTTGTAAGGGTTGGCCCCAACCCCAAGTTTGCCCCGGTTGCTGGATATCACTGGTTTGATGGAGATGGTATGATTCATGGTATGCGGATCAAGGATGGAAAAGCAACATATGTCTCCCGCTATATAAAGACATCACGTCTTAAGCAAGAAGAGTACTTTGGAGGTGCCAAATTAATGAAGATTGGAGACCTTAAGGGGCTTTTTGGTTTACTCATGGTTTACATGCAGCAGCTGAGAGCTAAACTGAAAATATTAGATCTTTCATATGGACATGGGACAGGTAACACAGCTCTCATATATCACCATGGGAAACTTCTAGCTCTTTCAGAGGGAGACAAACCTTATGTTCTCAAAGTTTTGGAAGATGGAGATCTGCAAACAGTTGGCTTGTTGGATTATGACAAGAGATTGAAGCATTCCTTCACTGCTCATCCAAAGGTTGACCCTTTCACTGGGGAGATGTTTACATTTGGCTATTCGCATGATCCACCATATGTCACATACAGAGTAATTTCAAAGGATGGTTTCATGCATGATCCTATACCTATAACAGTAACAGCTCCTATCATGATGCATGATTTTGCCATTACTGAGAACTATGCAATTTTCATGGATCTTCCCTTGTATTTCAGACCAAAGGAAATGGTTAAGGAAGGCAAGCTGATATTCTCATTTGATGAAACAAAAAAAGCTCGATTTGGTGTCCTTCCACGTTATGCAAAGGATGATTTGCTAATCAGATGGTTTGAGCTTCCAAATTGCTTCATTTTCCATAATGCGAATGCTTGGGAGGAAGAGGACGAAGTTGTTTTAATGACATGCCGCCTTGAGAATCCAGATTTAGACATGGTCAGTGGACCTATCAAGAAAAAGCCTGACAACTTCAAAAATGAGCTGTATGAGATGAGATTCAACTTAAAAACCGGTTTAGCAACACAGAAGAAATTATCAGAATCAGATGTAGATTTTCCTAGGGTGAATGAGAGCTACACTGGAAGGAAGCAACGCTATGTCTATGGAACCACACTTGATAGCATTGTGAGGGTGACAGGGATAGTCAAGTTTGATCTGCATGCTGCACCTGAGGTTGGAAAAACGAAAATTGAGGTTGGAGGAAACGTCCAAGGCCTTTATGACCTGGGACCTGGTAGATTTGGTTCTGAGGCTATTTTTGTCCCCCGTGTTCCTGGTATTACTTCAGAAGAAGATGATGGCTACTTGGTATTCTTTGTACATGATGAGAATACTGGAAAATCAGCAATTCATGTACTAGATGCAAAAACAATGTCCAGTGATCCTGTTGCAATTGTTGAGTTGCCCCACAGAGTTCCATATGGATTCCATGCCTTCTTTGTGACAGAGGAGCAATTGCAAGAACAAGCTAAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

552

Amino Acids

62.3

Weight (kDa)

6.22

Isoelectric Point (pI)

28.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 62 - 540 1.2e-141 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccB1I GGYRCC 2 cut(s) 67, 428
AciI CCGC 4 cut(s) 5, 349, 382, 1087
AclWI GGATC 5 cut(s) 359, 743, 794, 885, 1553
AcoI YGGCCR 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 68, 1446
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 4 cut(s) 146, 419, 1497, 1533
AfiI CCNNNNNNNGG 4 cut(s) 89, 305, 716, 1352
AflII CTTAAG 2 cut(s) 404, 455
AgeI ACCGGT 1 cut(s) 1187
AgsI TTSAA 5 cut(s) 256, 676, 780, 1150, 1177
AhdI GACNNNNNGTC 1 cut(s) 399
AjiI CACGTC 1 cut(s) 401
AjnI CCWGG 3 cut(s) 1401, 1408, 1450
AjuI GAANNNNNNNTTGG 2 cut(s) 405, 437
Alw26I GTCTC 3 cut(s) 382, 444, 591
AlwI GGATC 5 cut(s) 359, 743, 794, 885, 1553
AlwNI CAGNNNCTG 1 cut(s) 496
AoxI GGCC 3 cut(s) 235, 277, 1390
ApeKI GCWGC 3 cut(s) 490, 493, 1340
ArsI GACNNNNNNTTYG 2 cut(s) 1535, 1567
AseI ATTAAT 1 cut(s) 437
AsiGI ACCGGT 1 cut(s) 1187
AspA2I CCTAGG 1 cut(s) 1235
AspS9I GGNCC 4 cut(s) 220, 278, 1121, 1406
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 5 cut(s) 88, 155, 557, 1252, 1321
AvaII GGWCC 3 cut(s) 220, 1121, 1406
AvrII CCTAGG 1 cut(s) 1235
AxyI CCTNAGG 1 cut(s) 1347
BanI GGYRCC 2 cut(s) 67, 428
BbvI GCAGC 3 cut(s) 502, 505, 1327
BccI CCATC 8 cut(s) 246, 317, 323, 353, 623, 779, 999, 1469
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 3 cut(s) 1403, 1410, 1452
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 382, 444, 591
BfaI CTAG 3 cut(s) 581, 1236, 1535
BfrI CTTAAG 2 cut(s) 404, 455
BglII AGATCT 2 cut(s) 520, 634
BisI GCNGC 4 cut(s) 491, 494, 1087, 1341
BlnI CCTAGG 1 cut(s) 1235
BlsI GCNGC 4 cut(s) 492, 495, 1088, 1342
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 4 cut(s) 299, 1403, 1410, 1452
Bme18I GGWCC 3 cut(s) 220, 1121, 1406
BmeRI GACNNNNNGTC 1 cut(s) 399
BmgBI CACGTC 1 cut(s) 401
BmgT120I GGNCC 4 cut(s) 220, 278, 1121, 1406
BmiI GGNNCC 5 cut(s) 69, 280, 430, 1285, 1407
BmrFI CCNGG 4 cut(s) 299, 1403, 1410, 1452
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 2 cut(s) 825, 1528
BmuI ACTGGG 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 1112
BpuMI CCSGG 1 cut(s) 299
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 5 cut(s) 297, 568, 1235, 1387, 1402
BsaWI WCCGGW 2 cut(s) 190, 1187
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 1368, 1398
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 305, 716, 1352
Bse118I RCCGGY 1 cut(s) 1187
Bse1I ACTGG 6 cut(s) 111, 320, 721, 1261, 1516, 1554
Bse21I CCTNAGG 1 cut(s) 1347
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 3 cut(s) 1403, 1410, 1452
BseDI CCNNGG 5 cut(s) 297, 568, 1235, 1387, 1402
BseGI GGATG 4 cut(s) 364, 694, 790, 994
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 4 cut(s) 89, 305, 716, 1352
BseMII CTCAG 5 cut(s) 170, 488, 846, 1338, 1416
BseNI ACTGG 6 cut(s) 111, 320, 721, 1261, 1516, 1554
BseRI GAGGAG 1 cut(s) 1640
BseXI GCAGC 3 cut(s) 502, 505, 1327
BsgI GTGCAG 1 cut(s) 1326
BshFI GGCC 3 cut(s) 237, 279, 1392
BshNI GGYRCC 2 cut(s) 67, 428
BshTI ACCGGT 1 cut(s) 1187
BsiSI CCGG 4 cut(s) 177, 191, 299, 1188
BslFI GGGAC 3 cut(s) 553, 1419, 1424
BslI CCNNNNNNNGG 4 cut(s) 89, 305, 716, 1352
BsmAI GTCTC 3 cut(s) 382, 444, 591
BsmFI GGGAC 3 cut(s) 553, 1419, 1424
BsmI GAATGC 2 cut(s) 679, 1051
BsnI GGCC 3 cut(s) 237, 279, 1392
Bso31I GGTCTC 1 cut(s) 444
Bsp1407I TGTACA 1 cut(s) 1495
Bsp143I GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
Bsp19I CCATGG 1 cut(s) 568
BspACI CCGC 4 cut(s) 5, 349, 382, 1087
BspANI GGCC 3 cut(s) 237, 279, 1392
BspCNI CTCAG 5 cut(s) 169, 489, 847, 1339, 1417
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 5 cut(s) 69, 280, 430, 1285, 1407
BspPI GGATC 5 cut(s) 359, 743, 794, 885, 1553
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 2 cut(s) 67, 428
BspTI CTTAAG 2 cut(s) 404, 455
BspTNI GGTCTC 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 1187
BsrGI TGTACA 1 cut(s) 1495
BsrI ACTGG 6 cut(s) 111, 320, 721, 1261, 1516, 1554
BssAI RCCGGY 1 cut(s) 1187
BssECI CCNNGG 5 cut(s) 297, 568, 1235, 1387, 1402
BssMI GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 3 cut(s) 568, 1235, 1387
Bst2UI CCWGG 3 cut(s) 1403, 1410, 1452
Bst4CI ACNGT 3 cut(s) 152, 646, 817
Bst6I CTCTTC 3 cut(s) 44, 408, 1053
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 2 cut(s) 404, 455
BstAUI TGTACA 1 cut(s) 1495
BstC8I GCNNGC 2 cut(s) 923, 1338
BstDEI CTNAG 7 cut(s) 156, 216, 497, 855, 1347, 1425, 1647
BstDSI CCRYGG 1 cut(s) 568
BstF5I GGATG 4 cut(s) 364, 694, 790, 994
BstKTI GATC 8 cut(s) 354, 523, 637, 751, 802, 880, 1333, 1561
BstMAI GTCTC 3 cut(s) 382, 444, 591
BstMBI GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 3 cut(s) 1403, 1410, 1452
BstNSI RCATGY 3 cut(s) 490, 1086, 1340
BstSCI CCNGG 4 cut(s) 297, 1401, 1408, 1450
BstV1I GCAGC 3 cut(s) 502, 505, 1327
BstX2I RGATCY 3 cut(s) 520, 634, 877
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 3 cut(s) 520, 634, 877
Bsu36I CCTNAGG 1 cut(s) 1347
BsuRI GGCC 3 cut(s) 237, 279, 1392
BtgI CCRYGG 1 cut(s) 568
BtrI CACGTC 1 cut(s) 401
BtsCI GGATG 4 cut(s) 364, 694, 790, 994
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 6 cut(s) 313, 687, 714, 1123, 1254, 1561
Cac8I GCNNGC 2 cut(s) 923, 1338
CaiI CAGNNNCTG 1 cut(s) 496
CciI TCATGA 1 cut(s) 831
Cfr10I RCCGGY 1 cut(s) 1187
Cfr13I GGNCC 4 cut(s) 220, 278, 1121, 1406
CseI GACGC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 1408
Csp6I GTAC 4 cut(s) 145, 418, 1496, 1532
CspAI ACCGGT 1 cut(s) 1187
CviQI GTAC 4 cut(s) 145, 418, 1496, 1532
DdeI CTNAG 7 cut(s) 156, 216, 497, 855, 1347, 1425, 1647
DpnI GATC 8 cut(s) 353, 522, 636, 750, 801, 879, 1332, 1560
DpnII GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
DrdI GACNNNNNNGTC 1 cut(s) 227
DriI GACNNNNNGTC 1 cut(s) 399
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 44, 408, 1053
Eam1105I GACNNNNNGTC 1 cut(s) 399
EarI CTCTTC 3 cut(s) 44, 408, 1053
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 568, 1235, 1387
Eco147I AGGCCT 1 cut(s) 1392
Eco31I GGTCTC 1 cut(s) 444
Eco32I GATATC 1 cut(s) 311
Eco47I GGWCC 3 cut(s) 220, 1121, 1406
Eco57I CTGAAG 2 cut(s) 68, 1446
Eco81I CCTNAGG 1 cut(s) 1347
EcoO109I RGGNCCY 2 cut(s) 220, 1406
EcoRII CCWGG 3 cut(s) 1401, 1408, 1450
EcoRV GATATC 1 cut(s) 311
EcoT14I CCWWGG 3 cut(s) 568, 1235, 1387
EcoT22I ATGCAT 2 cut(s) 798, 840
ErhI CCWWGG 3 cut(s) 568, 1235, 1387
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 3 cut(s) 553, 1419, 1424
FauI CCCGC 1 cut(s) 389
FauNDI CATATG 4 cut(s) 373, 529, 757, 1597
Fnu4HI GCNGC 4 cut(s) 491, 494, 1087, 1341
FokI GGATG 4 cut(s) 371, 681, 797, 1001
Fsp4HI GCNGC 4 cut(s) 491, 494, 1087, 1341
FspBI CTAG 3 cut(s) 581, 1236, 1535
GluI GCNGC 4 cut(s) 491, 494, 1087, 1341
HaeIII GGCC 3 cut(s) 237, 279, 1392
HapII CCGG 4 cut(s) 177, 191, 299, 1188
HgaI GACGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 93
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 6 cut(s) 73, 337, 1096, 1173, 1217, 1602
HpaII CCGG 4 cut(s) 177, 191, 299, 1188
HphI GGTGA 5 cut(s) 88, 155, 557, 1252, 1321
Hpy166II GTNNAC 6 cut(s) 112, 473, 484, 706, 729, 1121
Hpy188I TCNGA 7 cut(s) 592, 896, 1004, 1216, 1222, 1426, 1465
Hpy188III TCNNGA 6 cut(s) 16, 26, 245, 832, 1100, 1129
Hpy8I GTNNAC 6 cut(s) 112, 473, 484, 706, 729, 1121
HpyAV CCTTC 6 cut(s) 31, 694, 911, 980, 1254, 1621
HpyCH4III ACNGT 3 cut(s) 152, 646, 817
HpyCH4IV ACGT 3 cut(s) 400, 976, 1383
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 7 cut(s) 156, 216, 497, 855, 1347, 1425, 1647
HpySE526I ACGT 3 cut(s) 400, 976, 1383
Hsp92I GRCGYC 1 cut(s) 93
Kzo9I GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 3 cut(s) 193, 829, 1627
Lsp1109I GCAGC 3 cut(s) 502, 505, 1327
LweI GCATC 2 cut(s) 825, 1528
MabI ACCWGGT 1 cut(s) 1408
MaeI CTAG 3 cut(s) 581, 1236, 1535
MaeII ACGT 3 cut(s) 400, 976, 1383
MaeIII GTNAC 6 cut(s) 94, 545, 760, 817, 1309, 1618
MalI GATC 8 cut(s) 353, 522, 636, 750, 801, 879, 1332, 1560
MboI GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
MfeI CAATTG 2 cut(s) 1569, 1631
MflI RGATCY 3 cut(s) 520, 634, 877
MluCI AATT 9 cut(s) 434, 774, 867, 1021, 1208, 1365, 1524, 1569, 1631
MmeI TCCRAC 3 cut(s) 636, 1333, 1354
Mph1103I ATGCAT 2 cut(s) 798, 840
MseI TTAA 6 cut(s) 405, 437, 456, 912, 1076, 1181
MslI CAYNNNNRTG 2 cut(s) 30, 65
MspA1I CMGCKG 1 cut(s) 496
MspCI CTTAAG 2 cut(s) 404, 455
MspI CCGG 4 cut(s) 177, 191, 299, 1188
MspR9I CCNGG 4 cut(s) 299, 1403, 1410, 1452
MunI CAATTG 2 cut(s) 1569, 1631
Mva1269I GAATGC 2 cut(s) 679, 1051
MvaI CCWGG 3 cut(s) 1403, 1410, 1452
MwoI GCNNNNNNNGC 1 cut(s) 302
NciI CCSGG 1 cut(s) 299
NcoI CCATGG 1 cut(s) 568
NdeI CATATG 4 cut(s) 373, 529, 757, 1597
NdeII GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
NlaIV GGNNCC 5 cut(s) 69, 280, 430, 1285, 1407
NmeAIII GCCGAG 1 cut(s) 108
NmuCI GTSAC 4 cut(s) 94, 760, 1309, 1618
NsiI ATGCAT 2 cut(s) 798, 840
NspI RCATGY 3 cut(s) 490, 1086, 1340
PaeI GCATGC 1 cut(s) 1340
PagI TCATGA 1 cut(s) 831
PceI AGGCCT 1 cut(s) 1392
PciSI GCTCTTC 1 cut(s) 44
PctI GAATGC 2 cut(s) 679, 1051
PfeI GAWTC 6 cut(s) 73, 337, 1096, 1173, 1217, 1602
PflFI GACNNNGTC 1 cut(s) 1112
PinAI ACCGGT 1 cut(s) 1187
PkrI GCNGC 4 cut(s) 492, 495, 1088, 1342
PpuMI RGGWCCY 2 cut(s) 220, 1406
PshBI ATTAAT 1 cut(s) 437
Psp5II RGGWCCY 2 cut(s) 220, 1406
Psp6I CCWGG 3 cut(s) 1401, 1408, 1450
PspGI CCWGG 3 cut(s) 1401, 1408, 1450
PspN4I GGNNCC 5 cut(s) 69, 280, 430, 1285, 1407
PspPI GGNCC 4 cut(s) 220, 278, 1121, 1406
PspPPI RGGWCCY 2 cut(s) 220, 1406
PsrI GAACNNNNNNTAC 2 cut(s) 1405, 1437
PstNI CAGNNNCTG 1 cut(s) 496
PsuI RGATCY 3 cut(s) 520, 634, 877
PsyI GACNNNGTC 1 cut(s) 1112
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 4 cut(s) 146, 419, 1497, 1533
RsaNI GTAC 4 cut(s) 145, 418, 1496, 1532
RseI CAYNNNNRTG 2 cut(s) 30, 65
SapI GCTCTTC 1 cut(s) 44
SaqAI TTAA 6 cut(s) 405, 437, 456, 912, 1076, 1181
SatI GCNGC 4 cut(s) 491, 494, 1087, 1341
Sau3AI GATC 8 cut(s) 351, 520, 634, 748, 799, 877, 1330, 1558
Sau96I GGNCC 4 cut(s) 220, 278, 1121, 1406
ScaI AGTACT 1 cut(s) 419
ScrFI CCNGG 4 cut(s) 299, 1403, 1410, 1452
SexAI ACCWGGT 1 cut(s) 1408
SfaNI GCATC 2 cut(s) 825, 1528
SinI GGWCC 3 cut(s) 220, 1121, 1406
SmiMI CAYNNNNRTG 2 cut(s) 30, 65
SmlI CTYRAG 3 cut(s) 404, 455, 1091
SmoI CTYRAG 3 cut(s) 404, 455, 1091
SphI GCATGC 1 cut(s) 1340
Sse9I AATT 9 cut(s) 434, 774, 867, 1021, 1208, 1365, 1524, 1569, 1631
SseBI AGGCCT 1 cut(s) 1392
SsiI CCGC 4 cut(s) 5, 349, 382, 1087
SspI AATATT 1 cut(s) 516
SspMI CTAG 3 cut(s) 581, 1236, 1535
StuI AGGCCT 1 cut(s) 1392
StyD4I CCNGG 4 cut(s) 297, 1401, 1408, 1450
StyI CCWWGG 3 cut(s) 568, 1235, 1387
TaaI ACNGT 3 cut(s) 152, 646, 817
TaiI ACGT 3 cut(s) 403, 979, 1386
TaqI TCGA 2 cut(s) 15, 958
TasI AATT 9 cut(s) 434, 774, 867, 1021, 1208, 1365, 1524, 1569, 1631
TatI WGTACW 3 cut(s) 417, 1495, 1531
TauI GCSGC 1 cut(s) 1089
TfiI GAWTC 6 cut(s) 73, 337, 1096, 1173, 1217, 1602
Tru1I TTAA 6 cut(s) 405, 437, 456, 912, 1076, 1181
Tru9I TTAA 6 cut(s) 405, 437, 456, 912, 1076, 1181
TscAI CASTG 6 cut(s) 320, 694, 721, 1123, 1261, 1561
TseFI GTSAC 4 cut(s) 94, 760, 1309, 1618
TseI GCWGC 3 cut(s) 490, 493, 1340
Tsp45I GTSAC 4 cut(s) 94, 760, 1309, 1618
TspDTI ATGAA 8 cut(s) 329, 455, 516, 781, 862, 957, 1018, 1517
TspRI CASTG 6 cut(s) 320, 694, 721, 1123, 1261, 1561
Tth111I GACNNNGTC 1 cut(s) 1112
Vha464I CTTAAG 2 cut(s) 404, 455
VpaK11BI GGWCC 3 cut(s) 220, 1121, 1406
VspI ATTAAT 1 cut(s) 437
XceI RCATGY 3 cut(s) 490, 1086, 1340
XmaJI CCTAGG 1 cut(s) 1235
XspI CTAG 3 cut(s) 581, 1236, 1535
ZrmI AGTACT 1 cut(s) 419
Zsp2I ATGCAT 2 cut(s) 798, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.