Rh1AG019400

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0008044
Physical Location & Seq
Reverse (-)
3438127 .. 3570584
132458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG019400.1

Sequence Viewer

Length: 1167 bp
ATGGCGGAGGTGGTCGAGAAACGTCACGATGATGATGTGAAGGAGAAGCTGAAGAGCAACGGCATAGTGGTGCCGAATCCGAAGCCGAGCAAGGGCGTCACCTCCACACTGGTGGACACGGTTGAGAAGCTCATTGTCAAGTTGATGTACGACAGTTCTCAGCCTCACCACTATCTCGCCGGAAACTTTGCTCCGGTTATTGAGGAAACGCCTCCCACCAAGGACCTCCATGTCATCGGGCATCTTCCTGATTGCTTGAATGGAGAGTTTGTAAGGGTTGGCCCCAACCCCAAGTTTGCTCCAGTTGCTGGATATCACTGGTTTGATGGAGATGGTATGATTCATGGTATGCGCATCAAGGATGGGAAAGCAACATATGTTTCCCGCTATGTAAAGACATCACGTCTTAAGCAAGAAGAGTACTTTGGAGGTGCCAAATTTATGAAGGTCGGAGACCTTAAGGGGCTTTTTGGTTTACTCATGGTTTACATGCAGCAGCTGAGGGCTAAACTGAAAGTATTAGATCTTTCATATGGACATGGGACAGGTAACACAGCTCTCGTATATCACCATGGGAAACTTCTAGCTCTTTCAGAGGGAGACAAACCTTATGTTCTCAAAGTTCTGGAAGATGGAGATCTGCAAACAGTTGGCTTGTTGGATTATGACAAGAGATTGAAGCATTCCTTCACTGCTCATCCAAAGGTTGACCCTTTCACTGGGGAGATGTTTACATTTGGCTATTCGCATGATCCACCATACGTCACATACAGAGTAATTTCAAAGGATGGTTTCATGCATGATCCTATACCTATAACAGTACCAGCTCCTGTCATGATGCATGATTTTGCCATTACTGAGAACTATGCAATTTTCATGGATCTTCCCTTGTATTTCAGACCAAAGGAAATGGTTAAGGAAGGCAAGCTGATATTCTCATTTGATGAAACAAAAAAAGCTCGCTTTGGTGTCCTTCCACGATATGCAAAGGATGATTTGCTAATCAGATGGTTTGAGCTTCCAAATTGCTTCATTTTCCATAATGCTAATGCTTGGGAGGAAGAGGACGAAGTTGTTTTAATGACATGCCGCCTTGAGAATCCAGATTTAGACATGGTCAATGGACCGATCAAGAAAAAGCTTGACAACTTCAAAAATGAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

388

Amino Acids

44.16

Weight (kDa)

6.47

Isoelectric Point (pI)

28.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 63 - 371 5.1e-95 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 70, 431
AccB7I CCANNNNNTGG 1 cut(s) 308
AciI CCGC 3 cut(s) 5, 385, 1090
AclWI GGATC 3 cut(s) 746, 797, 888
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 3 cut(s) 149, 422, 822
AfiI CCNNNNNNNGG 3 cut(s) 92, 308, 719
AflII CTTAAG 2 cut(s) 407, 458
AgsI TTSAA 4 cut(s) 259, 679, 783, 1153
AhdI GACNNNNNGTC 1 cut(s) 402
AjiI CACGTC 1 cut(s) 404
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AleI CACNNNNGTG 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 447, 594
AlwI GGATC 3 cut(s) 746, 797, 888
AlwNI CAGNNNCTG 3 cut(s) 308, 499, 830
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 493, 496
ApoI RAATTY 1 cut(s) 437
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 3 cut(s) 223, 281, 1124
AsuHPI GGTGA 3 cut(s) 91, 158, 560
AvaII GGWCC 2 cut(s) 223, 1124
BanI GGYRCC 2 cut(s) 70, 431
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 505, 508
BccI CCATC 6 cut(s) 320, 326, 356, 626, 782, 1002
BceAI ACGGC 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 447, 594
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 1163
BfrI CTTAAG 2 cut(s) 407, 458
BglII AGATCT 2 cut(s) 523, 637
BisI GCNGC 3 cut(s) 494, 497, 1090
BlsI GCNGC 3 cut(s) 495, 498, 1091
BmcAI AGTACT 1 cut(s) 422
Bme18I GGWCC 2 cut(s) 223, 1124
BmeRI GACNNNNNGTC 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 223, 281, 1124
BmiI GGNNCC 3 cut(s) 72, 283, 433
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 3 cut(s) 250, 363, 828
BmuI ACTGGG 1 cut(s) 729
BpmI CTGGAG 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 1115
BsaBI GATNNNNATC 1 cut(s) 636
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 219, 571
BsaWI WCCGGW 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 308, 719
Bse1I ACTGG 4 cut(s) 114, 302, 323, 724
Bse8I GATNNNNATC 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 219, 571
BseGI GGATG 4 cut(s) 367, 697, 793, 997
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 3 cut(s) 92, 308, 719
BseMII CTCAG 3 cut(s) 173, 491, 849
BseNI ACTGG 4 cut(s) 114, 302, 323, 724
BseXI GCAGC 2 cut(s) 505, 508
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 2 cut(s) 70, 431
BsiSI CCGG 2 cut(s) 180, 194
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 3 cut(s) 92, 308, 719
BsmAI GTCTC 2 cut(s) 447, 594
BsmFI GGGAC 1 cut(s) 556
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 447
Bsp143I GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
Bsp19I CCATGG 1 cut(s) 571
BspACI CCGC 3 cut(s) 5, 385, 1090
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 3 cut(s) 172, 492, 850
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 72, 283, 433
BspPI GGATC 3 cut(s) 746, 797, 888
BspQI GCTCTTC 1 cut(s) 47
BspT107I GGYRCC 2 cut(s) 70, 431
BspTI CTTAAG 2 cut(s) 407, 458
BspTNI GGTCTC 1 cut(s) 447
BsrI ACTGG 4 cut(s) 114, 302, 323, 724
BssECI CCNNGG 2 cut(s) 219, 571
BssMI GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 219, 571
Bst4CI ACNGT 4 cut(s) 121, 155, 649, 820
Bst6I CTCTTC 3 cut(s) 47, 411, 1056
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 2 cut(s) 407, 458
BstC8I GCNNGC 2 cut(s) 926, 961
BstDEI CTNAG 3 cut(s) 159, 500, 858
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 4 cut(s) 367, 697, 793, 997
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 6 cut(s) 526, 640, 754, 805, 883, 1131
BstMAI GTCTC 2 cut(s) 447, 594
BstMBI GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNSI RCATGY 2 cut(s) 493, 1089
BstSFI CTRYAG 1 cut(s) 1163
BstV1I GCAGC 2 cut(s) 505, 508
BstX2I RGATCY 3 cut(s) 523, 637, 880
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 3 cut(s) 523, 637, 880
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 571
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 4 cut(s) 367, 697, 793, 997
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 4 cut(s) 107, 316, 690, 717
Cac8I GCNNGC 2 cut(s) 926, 961
CaiI CAGNNNCTG 3 cut(s) 308, 499, 830
CciI TCATGA 1 cut(s) 834
CfoI GCGC 1 cut(s) 354
Cfr13I GGNCC 3 cut(s) 223, 281, 1124
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 148, 421, 821
CviQI GTAC 3 cut(s) 148, 421, 821
DdeI CTNAG 3 cut(s) 159, 500, 858
DpnI GATC 6 cut(s) 525, 639, 753, 804, 882, 1130
DpnII GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
DrdI GACNNNNNNGTC 1 cut(s) 230
DriI GACNNNNNGTC 1 cut(s) 402
DseDI GACNNNNNNGTC 1 cut(s) 230
Eam1104I CTCTTC 3 cut(s) 47, 411, 1056
Eam1105I GACNNNNNGTC 1 cut(s) 402
EarI CTCTTC 3 cut(s) 47, 411, 1056
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 219, 571
Eco31I GGTCTC 1 cut(s) 447
Eco32I GATATC 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 223, 1124
Eco57I CTGAAG 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 223
EcoRV GATATC 1 cut(s) 314
EcoT14I CCWWGG 2 cut(s) 219, 571
EcoT22I ATGCAT 2 cut(s) 801, 843
ErhI CCWWGG 2 cut(s) 219, 571
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FaqI GGGAC 1 cut(s) 556
FauI CCCGC 1 cut(s) 392
FauNDI CATATG 2 cut(s) 376, 532
Fnu4HI GCNGC 3 cut(s) 494, 497, 1090
FokI GGATG 4 cut(s) 374, 684, 800, 1004
Fsp4HI GCNGC 3 cut(s) 494, 497, 1090
FspAI RTGCGCAY 1 cut(s) 353
FspBI CTAG 1 cut(s) 584
FspI TGCGCA 1 cut(s) 353
GlaI GCGC 1 cut(s) 353
GluI GCNGC 3 cut(s) 494, 497, 1090
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 1 cut(s) 282
HapII CCGG 2 cut(s) 180, 194
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 354
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 3 cut(s) 76, 340, 1099
HpaII CCGG 2 cut(s) 180, 194
HphI GGTGA 3 cut(s) 91, 158, 560
Hpy166II GTNNAC 5 cut(s) 115, 476, 487, 709, 732
Hpy188I TCNGA 5 cut(s) 81, 452, 595, 899, 1007
Hpy188III TCNNGA 7 cut(s) 16, 26, 248, 626, 835, 1103, 1132
Hpy8I GTNNAC 5 cut(s) 115, 476, 487, 709, 732
HpyAV CCTTC 5 cut(s) 34, 439, 697, 914, 983
HpyCH4III ACNGT 4 cut(s) 121, 155, 649, 820
HpyCH4IV ACGT 3 cut(s) 22, 403, 762
HpyCH4V TGCA 6 cut(s) 493, 643, 799, 841, 869, 986
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 159, 500, 858
HpySE526I ACGT 3 cut(s) 22, 403, 762
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 1 cut(s) 352
Kzo9I GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 3 cut(s) 196, 304, 832
Lsp1109I GCAGC 2 cut(s) 505, 508
LweI GCATC 3 cut(s) 250, 363, 828
MaeI CTAG 1 cut(s) 584
MaeII ACGT 3 cut(s) 22, 403, 762
MaeIII GTNAC 4 cut(s) 23, 97, 548, 763
MalI GATC 6 cut(s) 525, 639, 753, 804, 882, 1130
MboI GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
MboII GAAGA 6 cut(s) 64, 236, 428, 641, 875, 1073
MflI RGATCY 3 cut(s) 523, 637, 880
MluCI AATT 4 cut(s) 437, 777, 870, 1024
MmeI TCCRAC 2 cut(s) 430, 639
Mph1103I ATGCAT 2 cut(s) 801, 843
MseI TTAA 4 cut(s) 408, 459, 915, 1079
MslI CAYNNNNRTG 3 cut(s) 30, 68, 110
MspA1I CMGCKG 1 cut(s) 499
MspCI CTTAAG 2 cut(s) 407, 458
MspI CCGG 2 cut(s) 180, 194
Mva1269I GAATGC 1 cut(s) 682
MwoI GCNNNNNNNGC 1 cut(s) 305
NcoI CCATGG 1 cut(s) 571
NdeI CATATG 2 cut(s) 376, 532
NdeII GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
NlaIV GGNNCC 3 cut(s) 72, 283, 433
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 3 cut(s) 23, 97, 763
NsbI TGCGCA 1 cut(s) 353
NsiI ATGCAT 2 cut(s) 801, 843
NspI RCATGY 2 cut(s) 493, 1089
OliI CACNNNNGTG 1 cut(s) 110
PagI TCATGA 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 47
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 3 cut(s) 76, 340, 1099
PflFI GACNNNGTC 1 cut(s) 1115
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 3 cut(s) 495, 498, 1091
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
PspN4I GGNNCC 3 cut(s) 72, 283, 433
PspPI GGNCC 3 cut(s) 223, 281, 1124
PspPPI RGGWCCY 1 cut(s) 223
PstNI CAGNNNCTG 3 cut(s) 308, 499, 830
PsuI RGATCY 3 cut(s) 523, 637, 880
PsyI GACNNNGTC 1 cut(s) 1115
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 3 cut(s) 149, 422, 822
RsaNI GTAC 3 cut(s) 148, 421, 821
RseI CAYNNNNRTG 3 cut(s) 30, 68, 110
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 4 cut(s) 408, 459, 915, 1079
SatI GCNGC 3 cut(s) 494, 497, 1090
Sau3AI GATC 6 cut(s) 523, 637, 751, 802, 880, 1128
Sau96I GGNCC 3 cut(s) 223, 281, 1124
ScaI AGTACT 1 cut(s) 422
SfaNI GCATC 3 cut(s) 250, 363, 828
SfcI CTRYAG 1 cut(s) 1163
SinI GGWCC 2 cut(s) 223, 1124
SmiMI CAYNNNNRTG 3 cut(s) 30, 68, 110
SmlI CTYRAG 3 cut(s) 407, 458, 1094
SmoI CTYRAG 3 cut(s) 407, 458, 1094
Sse9I AATT 4 cut(s) 437, 777, 870, 1024
SsiI CCGC 3 cut(s) 5, 385, 1090
SspMI CTAG 1 cut(s) 584
StyI CCWWGG 2 cut(s) 219, 571
TaaI ACNGT 4 cut(s) 121, 155, 649, 820
TaiI ACGT 3 cut(s) 25, 406, 765
TaqI TCGA 1 cut(s) 15
TaqII GACCGA 1 cut(s) 1141
TasI AATT 4 cut(s) 437, 777, 870, 1024
TatI WGTACW 1 cut(s) 420
TauI GCSGC 1 cut(s) 1092
TfiI GAWTC 3 cut(s) 76, 340, 1099
Tru1I TTAA 4 cut(s) 408, 459, 915, 1079
Tru9I TTAA 4 cut(s) 408, 459, 915, 1079
TscAI CASTG 4 cut(s) 114, 323, 697, 724
TseFI GTSAC 3 cut(s) 23, 97, 763
TseI GCWGC 2 cut(s) 493, 496
Tsp45I GTSAC 3 cut(s) 23, 97, 763
TspDTI ATGAA 7 cut(s) 332, 458, 519, 784, 865, 960, 1021
TspRI CASTG 4 cut(s) 114, 323, 697, 724
Tth111I GACNNNGTC 1 cut(s) 1115
Van91I CCANNNNNTGG 1 cut(s) 308
Vha464I CTTAAG 2 cut(s) 407, 458
VpaK11BI GGWCC 2 cut(s) 223, 1124
XapI RAATTY 1 cut(s) 437
XceI RCATGY 2 cut(s) 493, 1089
XspI CTAG 1 cut(s) 584
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 2 cut(s) 801, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.