MD07G1011500.v1.1

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008044
Physical Location & Seq
Reverse (-)
1033256 .. 1039635
6380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1011500.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGCGATCAAAAAACCGCCTACCACTTCATCATTTGGTCCGTTTCCACCACACAGACTGGCTCACCCACTGGCTCCGACCAAGGACCAGAAACCAGACAGCAATGGCGGATGTCGAAGTTAGCGACGAGAAGCAGCAGCTCAAGGGCAGCGGCATAGTGATCCCCAACCCAAAACCCAACAAGGGTTATGCTTCAAAAGTCGTGGACTTGATGGAGAAGCTGATTGTGAAGTGGATGTACGACTCTTCTCAGCCTCACCACTACCTCGCCGGCAACTTTGCTCCGGTGGTTGATGAAACTCCTCCCACCACCAACCTCCCTGTCATCGGCCATCTTCCTGAGTGCTTGAATGGAGAGTTTGTCAGGGTGGGGCCCAACCCAAAGTTTGCACCAGTTGCTGGATATCACTGGTTTGATGGAGATGGTATGGTTCATGGTATGCGCATCAAAGATGGCAAAGCAACTTATGTATCTCGCTACGTTAGGACGTCACGTCTTAAGCAAGAAGAATATTTTGGAGGTGCTAAATTTATGAAGATTGGAGACCTTAAGGGGTTTTTTGGCTTATTCATGGTGAACTTGCAAATGCTAAGAGCAAAACTGAAAATATTTGATATGTCTTATGGAAATGGGACAGGTAACACAGCTCTCGTATATCACCATGGGAAGCTTCTAGCTCTTTCAGAGGCAGATAAACCTTATGTCCTCAAAGTTTTGGAAGATGGAGATCTGCAAACAGTTGGCTTGCTGGATTATGACAAGAGACTGACACATTCCTTCACTGCTCATCCAAAGGTTGACCCAGTCACTGGTGAGATGTTTACCTTTGGCTACTCACATGCACCGCCATATGTCACTTATAGAGTTATTTCAAAGGATGGTTTCATGCATGACCCTATACCGATAACAATAGCAGACCCCGTCATGATGCATGATTTTGCAATTACTGAGAACTATGCAATTTTCATGGATCTCCCCCTGATCTTCAAACCAAAGGAAATGGTGAAGGAAAAAAAGTTTATATTCACATTTGATCAAACAAAAATAGCTCGCTTTGGTTTACTTCCACGGTATGCAAAGAATGAGTCGCTAATCAGATGGTTCGAGCTTCCAAATTGCTTCATTTTCCATAATGCTAATGCTTGGGAGGAGGGGGATGAAGTTGTTTTAATCACATGCCGGCTTGATAATCTTGATTTGGACATGGTCAATGGGTCTGTCAAAGAAAAGCTTGAAAACTTCAAAAATGAGCTGTACGAGATGAGATTCAACTTGAAAACTGGTCTAGCATCCCAAAAGAAACTATCAGAATCGGCTGTAGATTTTCCCAGGGTGAATGAGAGCTACACTGGCAGGAAGCAGCGTTATGTGTACGGAACCTTACTAGACAGCATTGCCAAGGTTACAGGGGTAGCCAAGTTTGATTTGCATGCTGAACCAGAGGTCGGAAAAACAAAAATTGAGGTTGGAGGAAATGTCCAAGGCCTCTATGACCTGGGACCTGGCAGATTTGGTTCTGAAGCTATTTTTGTCCCTCGTATTCCTGGCGTTACTTCTGAAGAAGATGATGGCTACTTAATACTCTTTGTTCATGACGAGAACACTGGAAAATCAGCAGTTCATGTAGTTGATGCAAAGACAATGTCATCAGAGCCCGTTGCAGTCGTGGAATTGCCACACAGAGTTCCATATGGTTTCCATGCCTTCTTCGTGACAGAGGAGCAACTGCAAGAACAAGCAAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

583

Amino Acids

66.02

Weight (kDa)

6.77

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 93 - 571 9e-142 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 491
Acc16I TGCGCA 1 cut(s) 443
AccB7I CCANNNNNTGG 2 cut(s) 398, 809
AciI CCGC 4 cut(s) 16, 107, 150, 845
AclWI GGATC 2 cut(s) 154, 978
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 2 cut(s) 1539, 1578
AcyI GRCGYC 1 cut(s) 488
AfaI GTAC 3 cut(s) 239, 1256, 1373
AfiI CCNNNNNNNGG 5 cut(s) 182, 326, 398, 809, 1445
AflII CTTAAG 2 cut(s) 497, 548
AgsI TTSAA 8 cut(s) 195, 349, 873, 988, 1235, 1243, 1270, 1276
AhdI GACNNNNNGTC 1 cut(s) 492
AjiI CACGTC 1 cut(s) 494
AjnI CCWGG 4 cut(s) 1328, 1494, 1501, 1543
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
Alw26I GTCTC 2 cut(s) 537, 757
AlwI GGATC 2 cut(s) 154, 978
AlwNI CAGNNNCTG 2 cut(s) 398, 809
AoxI GGCC 3 cut(s) 328, 371, 1483
ApaI GGGCCC 1 cut(s) 375
ApeKI GCWGC 4 cut(s) 133, 136, 147, 1360
ApoI RAATTY 1 cut(s) 527
ArsI GACNNNNNNTTYG 4 cut(s) 1070, 1102, 1628, 1660
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 5 cut(s) 37, 84, 371, 372, 1499
AsuHPI GGTGA 7 cut(s) 55, 248, 586, 650, 824, 1015, 1345
AvaII GGWCC 3 cut(s) 37, 84, 1499
BaeGI GKGCMC 1 cut(s) 375
BaeI ACNNNNGTAYC 2 cut(s) 453, 486
BanII GRGCYC 2 cut(s) 375, 1656
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbvI GCAGC 4 cut(s) 145, 148, 159, 1372
BccI CCATC 9 cut(s) 205, 339, 410, 416, 446, 716, 872, 1092, 1562
BciT130I CCWGG 4 cut(s) 1330, 1496, 1503, 1545
BclI TGATCA 1 cut(s) 1033
BcoDI GTCTC 2 cut(s) 537, 757
BfaI CTAG 3 cut(s) 674, 1286, 1385
BfmI CTRYAG 1 cut(s) 1317
BfrI CTTAAG 2 cut(s) 497, 548
BglII AGATCT 1 cut(s) 727
BisI GCNGC 5 cut(s) 134, 137, 148, 151, 1361
BlsI GCNGC 5 cut(s) 135, 138, 149, 152, 1362
Bme1390I CCNGG 4 cut(s) 1330, 1496, 1503, 1545
Bme18I GGWCC 3 cut(s) 37, 84, 1499
BmeRI GACNNNNNGTC 1 cut(s) 492
BmgBI CACGTC 1 cut(s) 494
BmgT120I GGNCC 5 cut(s) 37, 84, 371, 372, 1499
BmiI GGNNCC 5 cut(s) 74, 372, 373, 1378, 1500
BmrFI CCNGG 4 cut(s) 1330, 1496, 1503, 1545
BmrI ACTGGG 1 cut(s) 797
BmsI GCATC 4 cut(s) 453, 918, 1298, 1621
BmuI ACTGGG 1 cut(s) 797
BpuEI CTTGAG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 8 cut(s) 80, 661, 1067, 1328, 1329, 1398, 1480, 1495
BsaWI WCCGGW 1 cut(s) 283
BsaXI ACNNNNNCTCC 4 cut(s) 345, 375, 1461, 1491
Bsc4I CCNNNNNNNGG 5 cut(s) 182, 326, 398, 809, 1445
Bse118I RCCGGY 2 cut(s) 269, 1179
Bse1I ACTGG 9 cut(s) 62, 74, 392, 413, 803, 814, 1285, 1354, 1609
Bse3DI GCAATG 2 cut(s) 108, 1392
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 4 cut(s) 1330, 1496, 1503, 1545
BseDI CCNNGG 8 cut(s) 80, 661, 1067, 1328, 1329, 1398, 1480, 1495
BseGI GGATG 6 cut(s) 115, 240, 787, 883, 1162, 1289
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 5 cut(s) 182, 326, 398, 809, 1445
BseMI GCAATG 2 cut(s) 108, 1392
BseMII CTCAG 3 cut(s) 263, 330, 939
BseNI ACTGG 9 cut(s) 62, 74, 392, 413, 803, 814, 1285, 1354, 1609
BseRI GAGGAG 3 cut(s) 291, 1163, 1733
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 4 cut(s) 145, 148, 159, 1372
BshFI GGCC 3 cut(s) 330, 373, 1485
BsiSI CCGG 3 cut(s) 270, 284, 1180
BslFI GGGAC 3 cut(s) 646, 1512, 1517
BslI CCNNNNNNNGG 5 cut(s) 182, 326, 398, 809, 1445
BsmAI GTCTC 2 cut(s) 537, 757
BsmFI GGGAC 3 cut(s) 646, 1512, 1517
BsnI GGCC 3 cut(s) 330, 373, 1485
Bso31I GGTCTC 1 cut(s) 537
Bsp120I GGGCCC 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 375, 1656
Bsp143I GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 4 cut(s) 16, 107, 150, 845
BspANI GGCC 3 cut(s) 330, 373, 1485
BspCNI CTCAG 3 cut(s) 262, 331, 940
BspHI TCATGA 2 cut(s) 924, 1591
BspLI GGNNCC 5 cut(s) 74, 372, 373, 1378, 1500
BspPI GGATC 2 cut(s) 154, 978
BspTI CTTAAG 2 cut(s) 497, 548
BspTNI GGTCTC 1 cut(s) 537
BsrDI GCAATG 2 cut(s) 108, 1392
BsrFI RCCGGY 2 cut(s) 269, 1179
BsrI ACTGG 9 cut(s) 62, 74, 392, 413, 803, 814, 1285, 1354, 1609
BssAI RCCGGY 2 cut(s) 269, 1179
BssECI CCNNGG 8 cut(s) 80, 661, 1067, 1328, 1329, 1398, 1480, 1495
BssMI GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
BssNI GRCGYC 1 cut(s) 488
BssT1I CCWWGG 4 cut(s) 80, 661, 1398, 1480
Bst2UI CCWGG 4 cut(s) 1330, 1496, 1503, 1545
Bst4CI ACNGT 2 cut(s) 739, 1071
Bst6I CTCTTC 1 cut(s) 250
BstACI GRCGYC 1 cut(s) 488
BstAFI CTTAAG 2 cut(s) 497, 548
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 5 cut(s) 271, 746, 1051, 1181, 1431
BstDEI CTNAG 4 cut(s) 249, 339, 590, 948
BstDSI CCRYGG 2 cut(s) 661, 1067
BstF5I GGATG 6 cut(s) 115, 240, 787, 883, 1162, 1289
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 6 cut(s) 8, 162, 730, 973, 984, 1036
BstMAI GTCTC 2 cut(s) 537, 757
BstMBI GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 395, 1350
BstNI CCWGG 4 cut(s) 1330, 1496, 1503, 1545
BstNSI RCATGY 3 cut(s) 842, 1179, 1433
BstSCI CCNGG 4 cut(s) 1328, 1494, 1501, 1543
BstSFI CTRYAG 1 cut(s) 1317
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 145, 148, 159, 1372
BstX2I RGATCY 2 cut(s) 727, 970
BstYI RGATCY 2 cut(s) 727, 970
BsuRI GGCC 3 cut(s) 330, 373, 1485
BtgI CCRYGG 2 cut(s) 661, 1067
BtrI CACGTC 1 cut(s) 494
BtsCI GGATG 6 cut(s) 115, 240, 787, 883, 1162, 1289
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 6 cut(s) 67, 406, 780, 807, 1347, 1602
Cac8I GCNNGC 5 cut(s) 271, 746, 1051, 1181, 1431
CaiI CAGNNNCTG 2 cut(s) 398, 809
CciI TCATGA 2 cut(s) 924, 1591
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 2 cut(s) 269, 1179
Cfr13I GGNCC 5 cut(s) 37, 84, 371, 372, 1499
Csp6I GTAC 3 cut(s) 238, 1255, 1372
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviQI GTAC 3 cut(s) 238, 1255, 1372
DdeI CTNAG 4 cut(s) 249, 339, 590, 948
DpnI GATC 6 cut(s) 7, 161, 729, 972, 983, 1035
DpnII GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 250
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 4 cut(s) 80, 661, 1398, 1480
Eco147I AGGCCT 1 cut(s) 1485
Eco24I GRGCYC 2 cut(s) 375, 1656
Eco31I GGTCTC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 404
Eco47I GGWCC 3 cut(s) 37, 84, 1499
Eco57I CTGAAG 2 cut(s) 1539, 1578
EcoO109I RGGNCCY 2 cut(s) 371, 1499
EcoRII CCWGG 4 cut(s) 1328, 1494, 1501, 1543
EcoRV GATATC 1 cut(s) 404
EcoT14I CCWWGG 4 cut(s) 80, 661, 1398, 1480
EcoT22I ATGCAT 2 cut(s) 891, 933
EcoT38I GRGCYC 2 cut(s) 375, 1656
ErhI CCWWGG 4 cut(s) 80, 661, 1398, 1480
FalI AAGNNNNNCTT 2 cut(s) 1215, 1247
FaqI GGGAC 3 cut(s) 646, 1512, 1517
FauNDI CATATG 2 cut(s) 850, 1690
FbaI TGATCA 1 cut(s) 1033
Fnu4HI GCNGC 5 cut(s) 134, 137, 148, 151, 1361
FokI GGATG 6 cut(s) 122, 247, 774, 890, 1169, 1276
FriOI GRGCYC 2 cut(s) 375, 1656
Fsp4HI GCNGC 5 cut(s) 134, 137, 148, 151, 1361
FspAI RTGCGCAY 1 cut(s) 443
FspBI CTAG 3 cut(s) 674, 1286, 1385
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 1 cut(s) 443
GluI GCNGC 5 cut(s) 134, 137, 148, 151, 1361
HaeIII GGCC 3 cut(s) 330, 373, 1485
HapII CCGG 3 cut(s) 270, 284, 1180
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 1 cut(s) 488
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 2 cut(s) 668, 1229
HinfI GANTC 4 cut(s) 242, 1085, 1266, 1310
HpaII CCGG 3 cut(s) 270, 284, 1180
HphI GGTGA 7 cut(s) 55, 248, 586, 650, 824, 1015, 1345
Hpy166II GTNNAC 6 cut(s) 205, 577, 799, 822, 1061, 1372
Hpy188I TCNGA 8 cut(s) 77, 685, 1097, 1309, 1448, 1519, 1558, 1651
Hpy188III TCNNGA 5 cut(s) 338, 925, 1193, 1592, 1711
Hpy8I GTNNAC 6 cut(s) 205, 577, 799, 822, 1061, 1372
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 787, 1000, 1714
HpyCH4III ACNGT 2 cut(s) 739, 1071
HpyCH4IV ACGT 3 cut(s) 480, 488, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 1350
HpyF3I CTNAG 4 cut(s) 249, 339, 590, 948
HpySE526I ACGT 3 cut(s) 480, 488, 493
Hsp92I GRCGYC 1 cut(s) 488
HspAI GCGC 1 cut(s) 442
KroI GCCGGC 2 cut(s) 269, 1179
KroNI GCCGGC 2 cut(s) 271, 1181
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
LmnI GCTCC 3 cut(s) 78, 286, 1720
Lsp1109I GCAGC 4 cut(s) 145, 148, 159, 1372
LweI GCATC 4 cut(s) 453, 918, 1298, 1621
MaeI CTAG 3 cut(s) 674, 1286, 1385
MaeII ACGT 3 cut(s) 480, 488, 493
MaeIII GTNAC 7 cut(s) 489, 638, 805, 853, 1402, 1549, 1711
MalI GATC 6 cut(s) 7, 161, 729, 972, 983, 1035
MboI GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
MboII GAAGA 9 cut(s) 237, 326, 518, 547, 731, 976, 1571, 1574, 1699
MflI RGATCY 2 cut(s) 727, 970
MhlI GDGCHC 2 cut(s) 375, 1656
MluCI AATT 6 cut(s) 527, 942, 960, 1114, 1458, 1670
MlyI GAGTC 2 cut(s) 236, 1094
MmeI TCCRAC 3 cut(s) 100, 1426, 1447
Mph1103I ATGCAT 2 cut(s) 891, 933
MroNI GCCGGC 2 cut(s) 269, 1179
MseI TTAA 4 cut(s) 498, 549, 1169, 1577
MspA1I CMGCKG 1 cut(s) 150
MspCI CTTAAG 2 cut(s) 497, 548
MspI CCGG 3 cut(s) 270, 284, 1180
MspR9I CCNGG 4 cut(s) 1330, 1496, 1503, 1545
MvaI CCWGG 4 cut(s) 1330, 1496, 1503, 1545
MwoI GCNNNNNNNGC 2 cut(s) 395, 1350
NaeI GCCGGC 2 cut(s) 271, 1181
NcoI CCATGG 1 cut(s) 661
NdeI CATATG 2 cut(s) 850, 1690
NdeII GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
NgoMIV GCCGGC 2 cut(s) 269, 1179
NlaIV GGNNCC 5 cut(s) 74, 372, 373, 1378, 1500
NmuCI GTSAC 4 cut(s) 489, 805, 853, 1711
NsbI TGCGCA 1 cut(s) 443
NsiI ATGCAT 2 cut(s) 891, 933
NspI RCATGY 3 cut(s) 842, 1179, 1433
PaeI GCATGC 1 cut(s) 1433
PagI TCATGA 2 cut(s) 924, 1591
PasI CCCWGGG 1 cut(s) 1329
PceI AGGCCT 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdiI GCCGGC 2 cut(s) 271, 1181
PfeI GAWTC 2 cut(s) 1266, 1310
PflFI GACNNNGTC 4 cut(s) 803, 920, 1205, 1642
PflMI CCANNNNNTGG 2 cut(s) 398, 809
PkrI GCNGC 5 cut(s) 135, 138, 149, 152, 1362
PleI GAGTC 2 cut(s) 236, 1093
PpsI GAGTC 2 cut(s) 236, 1093
PpuMI RGGWCCY 1 cut(s) 1499
Psp5II RGGWCCY 1 cut(s) 1499
Psp6I CCWGG 4 cut(s) 1328, 1494, 1501, 1543
PspGI CCWGG 4 cut(s) 1328, 1494, 1501, 1543
PspN4I GGNNCC 5 cut(s) 74, 372, 373, 1378, 1500
PspOMI GGGCCC 1 cut(s) 371
PspPI GGNCC 5 cut(s) 37, 84, 371, 372, 1499
PspPPI RGGWCCY 1 cut(s) 1499
PstNI CAGNNNCTG 2 cut(s) 398, 809
PsuI RGATCY 2 cut(s) 727, 970
PsyI GACNNNGTC 4 cut(s) 803, 920, 1205, 1642
RsaI GTAC 3 cut(s) 239, 1256, 1373
RsaNI GTAC 3 cut(s) 238, 1255, 1372
SaqAI TTAA 4 cut(s) 498, 549, 1169, 1577
SatI GCNGC 5 cut(s) 134, 137, 148, 151, 1361
Sau3AI GATC 6 cut(s) 5, 159, 727, 970, 981, 1033
Sau96I GGNCC 5 cut(s) 37, 84, 371, 372, 1499
SchI GAGTC 2 cut(s) 236, 1094
ScrFI CCNGG 4 cut(s) 1330, 1496, 1503, 1545
SduI GDGCHC 2 cut(s) 375, 1656
SfaNI GCATC 4 cut(s) 453, 918, 1298, 1621
SfcI CTRYAG 1 cut(s) 1317
SinI GGWCC 3 cut(s) 37, 84, 1499
SmlI CTYRAG 3 cut(s) 140, 497, 548
SmoI CTYRAG 3 cut(s) 140, 497, 548
SphI GCATGC 1 cut(s) 1433
Sse9I AATT 6 cut(s) 527, 942, 960, 1114, 1458, 1670
SseBI AGGCCT 1 cut(s) 1485
SsiI CCGC 4 cut(s) 16, 107, 150, 845
SspI AATATT 2 cut(s) 512, 609
SspMI CTAG 3 cut(s) 674, 1286, 1385
StuI AGGCCT 1 cut(s) 1485
StyD4I CCNGG 4 cut(s) 1328, 1494, 1501, 1543
StyI CCWWGG 4 cut(s) 80, 661, 1398, 1480
TaaI ACNGT 2 cut(s) 739, 1071
TaiI ACGT 3 cut(s) 483, 491, 496
TaqI TCGA 2 cut(s) 114, 1104
TasI AATT 6 cut(s) 527, 942, 960, 1114, 1458, 1670
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 1266, 1310
Tru1I TTAA 4 cut(s) 498, 549, 1169, 1577
Tru9I TTAA 4 cut(s) 498, 549, 1169, 1577
TscAI CASTG 6 cut(s) 74, 413, 787, 814, 1354, 1609
TseFI GTSAC 4 cut(s) 489, 805, 853, 1711
TseI GCWGC 4 cut(s) 133, 136, 147, 1360
Tsp45I GTSAC 4 cut(s) 489, 805, 853, 1711
TspGWI ACGGA 2 cut(s) 29, 1389
TspRI CASTG 6 cut(s) 74, 413, 787, 814, 1354, 1609
Tth111I GACNNNGTC 4 cut(s) 803, 920, 1205, 1642
Van91I CCANNNNNTGG 2 cut(s) 398, 809
Vha464I CTTAAG 2 cut(s) 497, 548
VpaK11BI GGWCC 3 cut(s) 37, 84, 1499
XapI RAATTY 1 cut(s) 527
XceI RCATGY 3 cut(s) 842, 1179, 1433
XspI CTAG 3 cut(s) 674, 1286, 1385
ZraI GACGTC 1 cut(s) 489
Zsp2I ATGCAT 2 cut(s) 891, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.