MD02G1315600.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006948
Physical Location & Seq
Forward (+)
37026833 .. 37029419
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1315600.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGAGCGGTCTTCTATACAAATACAATCCTAATTTTAAGGCCTCGGAGGGAGAGCCGAGGAAGAACCACGCGGCGGAGTTCATGGATTCGAATATTTTCCACCAGCAGCAGAGCTCCGGCTTAACGCGGTATCAGTCAGCTCCAAGCTCATTTCTGATGGAGCAGATGGACAACAATGGCGGCGGAACCCAGGATTTGCTGTATCTTCAACCTTCGAGCCCTGAAGTCGAAACGGTGCTGGCCAGGTTCATTTCTTCATGTAATGAACCAGATCAACGTGACAATGGTGTGCAGCAGCATCAGTTTGAAATCCAAGAGAGGCCGGTAAATGTGAAGGGAGAGGCAGAGGACTCTGTTTCTGAGCACATTAATGGTTACCCGAATTCTACTCATACGATGTACCAAGCTCCTCAAGGTCAGCAAGCTCATGGTTTAGACAGCAGCTCCTTTGCTGCAGTCAACTCTACGGGAATGGAGAATTCAATGATGCAATCGAAAATTGGGGTCGGAAATCGGTCTAATCTTGTTAGACAAAGTAGCTCTCCGGCTGGAGTCTTCCCAAACTCGACCGTTGACGATGGCTTTAACGTGATGAAGGACTCGGCAGGATATCGCGCAGGCAATGGTACAAATGGAGAAGCCAGTCCATCAACTTCTAGGTTTGGTAATCAACTGAGTTTCTCATCAAGGCAGTCTTCATACTCAGGTCGAATGCCTCGGATTGCTGAAGATGAGAATGGGAACTTGGGAGAAGATGAGAATGCTAATGGTAGCAATTCACCTTACCAGTCTAGCTTCCCAGATGATTCGTGGGACGATTCTTCATTTAATGACCTCAAAATAGCCAGAGACACTGATGGGAACAAGTTTTCTACTTCGACTGCATTCGAATCACAGAACAATGATTTTGGACACCGCAATCATGGCTCGACGCAACACTTGAGGTTTTCCAAACATTTTGAGATGCCTGCTATGGAGAAGTATTTGCAATTTGAAGATTCTATTCCTCGTAAAATTCGTGCCAAAAGAGGTTTCGCCACTCACCCGCGAAGCATTGCAGAGAGGATGAGAAGGACGCGGATTAGTGAAAGAATGAAGAAATTGCAGGAGCTTTTCCCAAACATGGACAAGCAAACAAACACGGCAGAAAAGTTGGACTTGGCGGTCGGGTTCATAAAAGACCTTCAGAAACAGGTTCAGACACTCAAGGATACGAAGGCCAAGTGCAGTTGTTCGAGCGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

416

Amino Acids

46.24

Weight (kDa)

5.92

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 351 - 396 2.2e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1163
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 5 cut(s) 71, 127, 615, 1048, 1078
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 3 cut(s) 382, 478, 1014
AcuI CTGAAG 3 cut(s) 243, 747, 1169
AfaI GTAC 2 cut(s) 401, 628
AfiI CCNNNNNNNGG 2 cut(s) 73, 1123
AgsI TTSAA 4 cut(s) 209, 308, 483, 995
AjnI CCWGG 2 cut(s) 189, 242
AjuI GAANNNNNNNTTGG 4 cut(s) 728, 760, 1016, 1048
AluBI AGCT 9 cut(s) 114, 140, 147, 407, 425, 444, 540, 795, 1111
AluI AGCT 9 cut(s) 114, 140, 147, 407, 425, 444, 540, 795, 1111
Alw21I GWGCWC 2 cut(s) 116, 366
Alw26I GTCTC 1 cut(s) 843
AoxI GGCC 4 cut(s) 39, 240, 320, 1218
ApeKI GCWGC 5 cut(s) 106, 292, 295, 441, 452
ApoI RAATTY 3 cut(s) 382, 478, 1014
AseI ATTAAT 1 cut(s) 369
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 617
AsuHPI GGTGA 2 cut(s) 771, 1034
AsuII TTCGAA 2 cut(s) 89, 888
BalI TGGCCA 1 cut(s) 242
BanII GRGCYC 2 cut(s) 116, 221
BbsI GAAGAC 3 cut(s) 2, 547, 687
Bbv12I GWGCWC 2 cut(s) 116, 366
BbvI GCAGC 5 cut(s) 118, 304, 307, 439, 453
BccI CCATC 5 cut(s) 151, 160, 572, 655, 851
BceAI ACGGC 1 cut(s) 1158
BciT130I CCWGG 2 cut(s) 191, 244
BciVI GTATCC 1 cut(s) 1204
BcoDI GTCTC 1 cut(s) 843
BfaI CTAG 2 cut(s) 657, 792
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 1204
BisI GCNGC 7 cut(s) 72, 107, 181, 293, 296, 442, 453
BlsI GCNGC 7 cut(s) 73, 108, 182, 294, 297, 443, 454
Bme1390I CCNGG 2 cut(s) 191, 244
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 2 cut(s) 191, 244
BmsI GCATC 3 cut(s) 307, 477, 954
BpiI GAAGAC 3 cut(s) 2, 547, 687
BpmI CTGGAG 1 cut(s) 570
Bpu14I TTCGAA 2 cut(s) 89, 888
BpuEI CTTGAG 3 cut(s) 396, 961, 1190
BsaJI CCNNGG 4 cut(s) 42, 56, 189, 716
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 1123
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 642, 787
Bse3DI GCAATG 2 cut(s) 628, 1053
BseBI CCWGG 2 cut(s) 191, 244
BseDI CCNNGG 4 cut(s) 42, 56, 189, 716
BseGI GGATG 1 cut(s) 1071
BseLI CCNNNNNNNGG 2 cut(s) 73, 1123
BseMI GCAATG 2 cut(s) 628, 1053
BseMII CTCAG 3 cut(s) 351, 665, 717
BseNI ACTGG 2 cut(s) 642, 787
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 5 cut(s) 118, 304, 307, 439, 453
BsgI GTGCAG 2 cut(s) 311, 1246
Bsh1236I CGCG 5 cut(s) 71, 127, 615, 1048, 1078
Bsh1285I CGRYCG 2 cut(s) 570, 1167
BshFI GGCC 4 cut(s) 41, 242, 322, 1220
BsiEI CGRYCG 2 cut(s) 570, 1167
BsiHKAI GWGCWC 2 cut(s) 116, 366
BsiSI CCGG 3 cut(s) 117, 323, 545
BslFI GGGAC 1 cut(s) 827
BslI CCNNNNNNNGG 2 cut(s) 73, 1123
BsmAI GTCTC 1 cut(s) 843
BsmFI GGGAC 1 cut(s) 827
BsmI GAATGC 3 cut(s) 717, 766, 884
BsnI GGCC 4 cut(s) 41, 242, 322, 1220
Bsp119I TTCGAA 2 cut(s) 89, 888
Bsp1286I GDGCHC 3 cut(s) 116, 221, 366
Bsp143I GATC 1 cut(s) 271
BspANI GGCC 4 cut(s) 41, 242, 322, 1220
BspCNI CTCAG 3 cut(s) 352, 666, 716
BspFNI CGCG 5 cut(s) 71, 127, 615, 1048, 1078
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 457
BspT104I TTCGAA 2 cut(s) 89, 888
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 628, 1053
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 2 cut(s) 642, 787
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 4 cut(s) 42, 56, 189, 716
BssMI GATC 1 cut(s) 271
Bst2UI CCWGG 2 cut(s) 191, 244
Bst4CI ACNGT 2 cut(s) 235, 571
BstBI TTCGAA 2 cut(s) 89, 888
BstC8I GCNNGC 4 cut(s) 240, 423, 619, 969
BstDEI CTNAG 3 cut(s) 360, 674, 703
BstEII GGTNACC 1 cut(s) 374
BstF5I GGATG 1 cut(s) 1071
BstFNI CGCG 5 cut(s) 71, 127, 615, 1048, 1078
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 843
BstMBI GATC 1 cut(s) 271
BstMCI CGRYCG 2 cut(s) 570, 1167
BstMWI GCNNNNNNNGC 1 cut(s) 924
BstNI CCWGG 2 cut(s) 191, 244
BstPI GGTNACC 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 189, 242
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 5 cut(s) 71, 127, 615, 1048, 1078
BstV1I GCAGC 5 cut(s) 118, 304, 307, 439, 453
BstV2I GAAGAC 3 cut(s) 2, 547, 687
BsuI GTATCC 1 cut(s) 1204
BsuRI GGCC 4 cut(s) 41, 242, 322, 1220
BtsCI GGATG 1 cut(s) 1071
BtsIMutI CAGTG 1 cut(s) 852
Cac8I GCNNGC 4 cut(s) 240, 423, 619, 969
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 1 cut(s) 322
CseI GACGC 2 cut(s) 940, 1084
Csp6I GTAC 2 cut(s) 400, 627
CviAII CATG 5 cut(s) 82, 258, 428, 923, 1123
CviQI GTAC 2 cut(s) 400, 627
DdeI CTNAG 3 cut(s) 360, 674, 703
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 1163
DseDI GACNNNNNNGTC 1 cut(s) 1163
EaeI YGGCCR 1 cut(s) 240
EciI GGCGGA 2 cut(s) 89, 198
Ecl136II GAGCTC 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 2 cut(s) 116, 221
Eco32I GATATC 1 cut(s) 611
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 3 cut(s) 243, 747, 1169
Eco91I GGTNACC 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 114
EcoO65I GGTNACC 1 cut(s) 374
EcoRI GAATTC 2 cut(s) 382, 478
EcoRII CCWGG 2 cut(s) 189, 242
EcoRV GATATC 1 cut(s) 611
EcoT38I GRGCYC 2 cut(s) 116, 221
FaeI CATG 5 cut(s) 85, 261, 431, 926, 1126
FalI AAGNNNNNCTT 4 cut(s) 679, 711, 1142, 1174
FaqI GGGAC 1 cut(s) 827
FatI CATG 5 cut(s) 81, 257, 427, 922, 1122
FauI CCCGC 1 cut(s) 1053
Fnu4HI GCNGC 7 cut(s) 72, 107, 181, 293, 296, 442, 453
FokI GGATG 1 cut(s) 1078
FriOI GRGCYC 2 cut(s) 116, 221
Fsp4HI GCNGC 7 cut(s) 72, 107, 181, 293, 296, 442, 453
FspBI CTAG 2 cut(s) 657, 792
GlaI GCGC 1 cut(s) 616
GluI GCNGC 7 cut(s) 72, 107, 181, 293, 296, 442, 453
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 4 cut(s) 41, 242, 322, 1220
HapII CCGG 3 cut(s) 117, 323, 545
HgaI GACGC 2 cut(s) 940, 1084
HhaI GCGC 1 cut(s) 617
Hin1II CATG 5 cut(s) 85, 261, 431, 926, 1126
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 2 cut(s) 460, 574
HindII GTYRAC 2 cut(s) 460, 574
HinfI GANTC 8 cut(s) 86, 350, 552, 599, 806, 818, 890, 998
HpaII CCGG 3 cut(s) 117, 323, 545
HphI GGTGA 2 cut(s) 771, 1034
Hpy166II GTNNAC 2 cut(s) 460, 574
Hpy188I TCNGA 7 cut(s) 46, 156, 361, 509, 720, 1188, 1200
Hpy8I GTNNAC 2 cut(s) 460, 574
Hpy99I CGWCG 1 cut(s) 934
HpyAV CCTTC 6 cut(s) 222, 328, 589, 1065, 1193, 1210
HpyCH4III ACNGT 2 cut(s) 235, 571
HpyCH4IV ACGT 2 cut(s) 277, 588
HpyCH4V TGCA 8 cut(s) 292, 455, 490, 884, 988, 1058, 1105, 1227
HpyF10VI GCNNNNNNNGC 1 cut(s) 924
HpyF3I CTNAG 3 cut(s) 360, 674, 703
HpySE526I ACGT 2 cut(s) 277, 588
Hsp92II CATG 5 cut(s) 85, 261, 431, 926, 1126
HspAI GCGC 1 cut(s) 615
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 6 cut(s) 119, 145, 160, 412, 449, 1108
Lsp1109I GCAGC 5 cut(s) 118, 304, 307, 439, 453
LweI GCATC 3 cut(s) 307, 477, 954
MaeI CTAG 2 cut(s) 657, 792
MaeII ACGT 2 cut(s) 277, 588
MaeIII GTNAC 2 cut(s) 278, 374
MalI GATC 1 cut(s) 273
MbiI CCGCTC 1 cut(s) 6
MboI GATC 1 cut(s) 271
MhlI GDGCHC 3 cut(s) 116, 221, 366
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 8 cut(s) 31, 382, 478, 498, 775, 989, 1014, 1100
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 3 cut(s) 344, 561, 593
MmeI TCCRAC 2 cut(s) 487, 1134
Mox20I TGGCCA 1 cut(s) 242
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 242
MseI TTAA 5 cut(s) 36, 122, 369, 585, 828
MslI CAYNNNNRTG 1 cut(s) 369
Msp20I TGGCCA 1 cut(s) 242
MspI CCGG 3 cut(s) 117, 323, 545
MspR9I CCNGG 2 cut(s) 191, 244
Mva1269I GAATGC 3 cut(s) 717, 766, 884
MvaI CCWGG 2 cut(s) 191, 244
MvnI CGCG 5 cut(s) 71, 127, 615, 1048, 1078
MwoI GCNNNNNNNGC 1 cut(s) 924
NdeII GATC 1 cut(s) 271
NlaIII CATG 5 cut(s) 85, 261, 431, 926, 1126
NlaIV GGNNCC 1 cut(s) 187
NmeAIII GCCGAG 2 cut(s) 81, 581
NmuCI GTSAC 1 cut(s) 278
NspV TTCGAA 2 cut(s) 89, 888
PceI AGGCCT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 1015
PctI GAATGC 3 cut(s) 717, 766, 884
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 5 cut(s) 86, 806, 818, 890, 998
PkrI GCNGC 7 cut(s) 73, 108, 182, 294, 297, 443, 454
PleI GAGTC 3 cut(s) 344, 560, 593
PpsI GAGTC 3 cut(s) 344, 560, 593
PshBI ATTAAT 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 116
Psp6I CCWGG 2 cut(s) 189, 242
PspEI GGTNACC 1 cut(s) 374
PspGI CCWGG 2 cut(s) 189, 242
PspN4I GGNNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 457
RsaI GTAC 2 cut(s) 401, 628
RsaNI GTAC 2 cut(s) 400, 627
RseI CAYNNNNRTG 1 cut(s) 369
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 5 cut(s) 36, 122, 369, 585, 828
SatI GCNGC 7 cut(s) 72, 107, 181, 293, 296, 442, 453
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 3 cut(s) 344, 561, 593
ScrFI CCNGG 2 cut(s) 191, 244
SduI GDGCHC 3 cut(s) 116, 221, 366
SfaNI GCATC 3 cut(s) 307, 477, 954
SfcI CTRYAG 1 cut(s) 453
SfuI TTCGAA 2 cut(s) 89, 888
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 3 cut(s) 411, 940, 1205
SmoI CTYRAG 3 cut(s) 411, 940, 1205
Sse9I AATT 8 cut(s) 31, 382, 478, 498, 775, 989, 1014, 1100
SseBI AGGCCT 1 cut(s) 41
SspI AATATT 1 cut(s) 94
SspMI CTAG 2 cut(s) 657, 792
SstI GAGCTC 1 cut(s) 116
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 189, 242
TaaI ACNGT 2 cut(s) 235, 571
TaiI ACGT 2 cut(s) 280, 591
TaqII GACCGA 1 cut(s) 504
TasI AATT 8 cut(s) 31, 382, 478, 498, 775, 989, 1014, 1100
TauI GCSGC 2 cut(s) 74, 183
TfiI GAWTC 5 cut(s) 86, 806, 818, 890, 998
Tru1I TTAA 5 cut(s) 36, 122, 369, 585, 828
Tru9I TTAA 5 cut(s) 36, 122, 369, 585, 828
TscAI CASTG 1 cut(s) 859
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 5 cut(s) 106, 292, 295, 441, 452
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 9 cut(s) 70, 238, 246, 279, 608, 687, 813, 1109, 1162
TspRI CASTG 1 cut(s) 859
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 3 cut(s) 382, 478, 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 807
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 2 cut(s) 657, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.