MD07G1006400.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006948
Physical Location & Seq
Reverse (-)
607842 .. 611161
3320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1006400.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGAGCAGTCTTTTATACAAATACAATCCTAATTTTAAGGCCTCAGAGGGAGAGCTGAGGAAGAACCACACTGCGGAGTTCATGGATTCCAATATTTTCCAGCAACAGCATCCTCAGCAGCAGCAGAGCTCCAACTTAACGCGGTATCGGTCAGCTCCTAGCTCATTTCTGATGGGGCTTATGGATAACAATGGCGGTAGCGGCGGCGGTGGAGTTGAGGATTTGCGGTATCTTCAACCTTCGAGCCCTGAAGTCGAAACGGTGCTAGCCAGATTCATTTCTTCATGTAATGAACCAGATCACCATGATAATGGTGTGCAGCATCATCAGATTGAAATCGAAGAGAGGCCAGTGAATGTGAAGGAAGAGGCAGGGGACTCTGTTTCTAATCACAATAATGGTTACTCGAATTCTACTCATATGATGTATCAAGCTCCTCAAGCTCCTCTGGCTCAACAAGTTCATGGTTTAGATAGCAGCTCCTTTGCTGCAGTCAACTCTACCGTAATGGAGAATTCGATGATGCAATCGAAAATTGGGGTTGGAAATCGGTCTAATCTTGTTAGACAAAGTAGCTCTCCGGCTGGATTCTTCCCTAACTTGACCGTTGACAATGGCTTCAATGTGATGAAAGACTCGGCAGGATATCGCGCAGGCAATGGTGTAAACGGAGAAGCCAGTCCATCAACTTCTAGGTTGGGTAAGCAACTGAATTTCTCATCTAGGCCATCTTCATACTCACATCGTATGCCTCGGATTGCTGAAAATGAGAATGGGAACTCGGGAGAAGGTAGCCAACCGCAACCGGATCATGGTTTAGGAAATGCTAATGGTAGCAATTCACATTACCACTCTAGCTTCCCAAATGATTCGTGGGTGAATTCTTCATTTAATGACCTCAAAAGAGCCAGAGACAATGAAGGGAACAAGTTTTCTACTTCGACTGCATTTGAATCACATAACAATGATTTTGGAAACCACAATCACGGTTTGACGCAACATTTGAGCTTGCCGAAAGATTTTGAGATGCCCGCTACGGAGAAGTATTTGCAATTTGAAGATTCCATTCCTTGTAAAATTCGTGCCAAAAGAGGCTTCGCCACTCACCCGCGAAGCATTGCAGAGAGGATGAGAAGGATGCGGATTAGTGAAAGAATGAAGAAATTGCAGGATCTTTTCCCGGACATGGACAAGCAAATAAACACGGCAGAAATGTTGGACTTGGCGGTTGAGTTCATAAAAGACCTTCAGAAACAGGTTAAGACACTCAAAGATACGAAGGCGAAGTGCAGTTGTTCGAGCAAACAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

438

Amino Acids

48.62

Weight (kDa)

7.02

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 372 - 417 1.4e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 142, 651, 1111
AclWI GGATC 2 cut(s) 816, 1179
AcsI RAATTY 5 cut(s) 409, 514, 712, 880, 1077
AcuI CTGAAG 2 cut(s) 270, 1232
AfiI CCNNNNNNNGG 3 cut(s) 73, 812, 1186
AgsI TTSAA 5 cut(s) 236, 335, 622, 953, 1058
AjuI GAANNNNNNNTTGG 2 cut(s) 1079, 1111
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 906
AlwI GGATC 2 cut(s) 816, 1179
Ama87I CYCGRG 1 cut(s) 781
AoxI GGCC 3 cut(s) 39, 347, 725
ApeKI GCWGC 5 cut(s) 118, 121, 319, 477, 488
ApoI RAATTY 5 cut(s) 409, 514, 712, 880, 1077
ArsI GACNNNNNNTTYG 2 cut(s) 985, 1017
AspLEI GCGC 1 cut(s) 653
AsuC2I CCSGG 1 cut(s) 1181
AsuHPI GGTGA 3 cut(s) 293, 889, 1097
AsuNHI GCTAGC 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 781
BanII GRGCYC 2 cut(s) 131, 248
Bbv12I GWGCWC 1 cut(s) 131
BbvCI CCTCAGC 2 cut(s) 56, 114
BbvI GCAGC 5 cut(s) 130, 133, 331, 475, 489
BccI CCATC 3 cut(s) 166, 691, 736
BceAI ACGGC 1 cut(s) 1221
BcnI CCSGG 1 cut(s) 1181
BcoDI GTCTC 1 cut(s) 906
BfaI CTAG 5 cut(s) 159, 266, 693, 723, 855
BfmI CTRYAG 1 cut(s) 489
BisI GCNGC 7 cut(s) 119, 122, 202, 205, 320, 478, 489
BlsI GCNGC 7 cut(s) 120, 123, 203, 206, 321, 479, 490
Bme1390I CCNGG 1 cut(s) 1181
BmeT110I CYCGRG 1 cut(s) 781
BmrFI CCNGG 1 cut(s) 1181
BmsI GCATC 5 cut(s) 118, 331, 513, 1017, 1128
BmtI GCTAGC 1 cut(s) 269
Bpu10I CCTNAGC 2 cut(s) 56, 114
BpuEI CTTGAG 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 1181
BsaBI GATNNNNATC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 752
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 812, 1186
Bse1I ACTGG 2 cut(s) 350, 678
Bse3DI GCAATG 2 cut(s) 664, 1116
Bse8I GATNNNNATC 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 752
BseGI GGATG 3 cut(s) 109, 1134, 1143
BseJI GATNNNNATC 1 cut(s) 335
BseLI CCNNNNNNNGG 3 cut(s) 73, 812, 1186
BseMI GCAATG 2 cut(s) 664, 1116
BseMII CTCAG 3 cut(s) 47, 57, 128
BseNI ACTGG 2 cut(s) 350, 678
BseRI GAGGAG 2 cut(s) 426, 435
BseXI GCAGC 5 cut(s) 130, 133, 331, 475, 489
BsgI GTGCAG 2 cut(s) 338, 1309
Bsh1236I CGCG 3 cut(s) 142, 651, 1111
BshFI GGCC 3 cut(s) 41, 349, 727
BsiHKAI GWGCWC 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 3 cut(s) 581, 806, 1181
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 3 cut(s) 73, 812, 1186
BsmAI GTCTC 1 cut(s) 906
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 3 cut(s) 41, 349, 727
BsoBI CYCGRG 1 cut(s) 781
Bsp1286I GDGCHC 2 cut(s) 131, 248
Bsp143I GATC 3 cut(s) 298, 808, 1171
BspANI GGCC 3 cut(s) 41, 349, 727
BspCNI CTCAG 3 cut(s) 48, 56, 127
BspFNI CGCG 3 cut(s) 142, 651, 1111
BspMAI CTGCAG 1 cut(s) 493
BspOI GCTAGC 1 cut(s) 269
BspPI GGATC 2 cut(s) 816, 1179
BsrDI GCAATG 2 cut(s) 664, 1116
BsrI ACTGG 2 cut(s) 350, 678
BssECI CCNNGG 1 cut(s) 752
BssMI GATC 3 cut(s) 298, 808, 1171
Bst4CI ACNGT 4 cut(s) 262, 505, 607, 989
Bst6I CTCTTC 2 cut(s) 336, 360
BstC8I GCNNGC 4 cut(s) 267, 655, 1010, 1032
BstDEI CTNAG 3 cut(s) 43, 56, 114
BstF5I GGATG 3 cut(s) 109, 1134, 1143
BstFNI CGCG 3 cut(s) 142, 651, 1111
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 3 cut(s) 301, 811, 1174
BstMAI GTCTC 1 cut(s) 906
BstMBI GATC 3 cut(s) 298, 808, 1171
BstMWI GCNNNNNNNGC 4 cut(s) 115, 201, 440, 449
BstSCI CCNGG 1 cut(s) 1179
BstSFI CTRYAG 1 cut(s) 489
BstUI CGCG 3 cut(s) 142, 651, 1111
BstV1I GCAGC 5 cut(s) 130, 133, 331, 475, 489
BstX2I RGATCY 1 cut(s) 1171
BstXI CCANNNNNNTGG 1 cut(s) 311
BstYI RGATCY 1 cut(s) 1171
BsuRI GGCC 3 cut(s) 41, 349, 727
BtsCI GGATG 3 cut(s) 109, 1134, 1143
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 357
Cac8I GCNNGC 4 cut(s) 267, 655, 1010, 1032
CfoI GCGC 1 cut(s) 653
CseI GACGC 1 cut(s) 1003
CviAII CATG 6 cut(s) 82, 285, 305, 464, 812, 1186
DdeI CTNAG 3 cut(s) 43, 56, 114
DpnI GATC 3 cut(s) 300, 810, 1173
DpnII GATC 3 cut(s) 298, 808, 1171
Eam1104I CTCTTC 2 cut(s) 336, 360
EarI CTCTTC 2 cut(s) 336, 360
Ecl136II GAGCTC 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 2 cut(s) 131, 248
Eco32I GATATC 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 270, 1232
Eco88I CYCGRG 1 cut(s) 781
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 3 cut(s) 409, 514, 880
EcoRV GATATC 1 cut(s) 647
EcoT38I GRGCYC 2 cut(s) 131, 248
FaeI CATG 6 cut(s) 85, 288, 308, 467, 815, 1189
FaqI GGGAC 1 cut(s) 389
FatI CATG 6 cut(s) 81, 284, 304, 463, 811, 1185
FauI CCCGC 2 cut(s) 1039, 1116
FauNDI CATATG 1 cut(s) 420
Fnu4HI GCNGC 7 cut(s) 119, 122, 202, 205, 320, 478, 489
FokI GGATG 3 cut(s) 96, 1141, 1150
FriOI GRGCYC 2 cut(s) 131, 248
Fsp4HI GCNGC 7 cut(s) 119, 122, 202, 205, 320, 478, 489
FspBI CTAG 5 cut(s) 159, 266, 693, 723, 855
GlaI GCGC 1 cut(s) 652
GluI GCNGC 7 cut(s) 119, 122, 202, 205, 320, 478, 489
HaeIII GGCC 3 cut(s) 41, 349, 727
HapII CCGG 3 cut(s) 581, 806, 1181
HgaI GACGC 1 cut(s) 1003
HhaI GCGC 1 cut(s) 653
Hin1II CATG 6 cut(s) 85, 288, 308, 467, 815, 1189
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HincII GTYRAC 2 cut(s) 496, 610
HindII GTYRAC 2 cut(s) 496, 610
HinfI GANTC 8 cut(s) 86, 273, 377, 588, 635, 869, 953, 1061
HpaII CCGG 3 cut(s) 581, 806, 1181
HphI GGTGA 3 cut(s) 293, 889, 1097
Hpy166II GTNNAC 3 cut(s) 496, 610, 667
Hpy188I TCNGA 5 cut(s) 46, 171, 330, 756, 1251
Hpy188III TCNNGA 1 cut(s) 783
Hpy8I GTNNAC 3 cut(s) 496, 610, 667
HpyAV CCTTC 7 cut(s) 249, 355, 782, 914, 1128, 1256, 1273
HpyCH4III ACNGT 4 cut(s) 262, 505, 607, 989
HpyCH4V TGCA 8 cut(s) 319, 491, 526, 947, 1051, 1121, 1168, 1290
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 201, 440, 449
HpyF3I CTNAG 3 cut(s) 43, 56, 114
Hsp92II CATG 6 cut(s) 85, 288, 308, 467, 815, 1189
HspAI GCGC 1 cut(s) 651
Kzo9I GATC 3 cut(s) 298, 808, 1171
LmnI GCTCC 5 cut(s) 134, 160, 439, 448, 485
Lsp1109I GCAGC 5 cut(s) 130, 133, 331, 475, 489
LweI GCATC 5 cut(s) 118, 331, 513, 1017, 1128
MaeI CTAG 5 cut(s) 159, 266, 693, 723, 855
MaeIII GTNAC 1 cut(s) 401
MalI GATC 3 cut(s) 300, 810, 1173
MboI GATC 3 cut(s) 298, 808, 1171
MflI RGATCY 1 cut(s) 1171
MhlI GDGCHC 2 cut(s) 131, 248
MlyI GAGTC 2 cut(s) 371, 629
MmeI TCCRAC 3 cut(s) 156, 523, 1197
MseI TTAA 4 cut(s) 36, 137, 891, 1260
MslI CAYNNNNRTG 3 cut(s) 309, 396, 963
MspI CCGG 3 cut(s) 581, 806, 1181
MspR9I CCNGG 1 cut(s) 1181
MvnI CGCG 3 cut(s) 142, 651, 1111
MwoI GCNNNNNNNGC 4 cut(s) 115, 201, 440, 449
NciI CCSGG 1 cut(s) 1181
NdeI CATATG 1 cut(s) 420
NdeII GATC 3 cut(s) 298, 808, 1171
NheI GCTAGC 1 cut(s) 265
NlaIII CATG 6 cut(s) 85, 288, 308, 467, 815, 1189
NmeAIII GCCGAG 1 cut(s) 617
PceI AGGCCT 1 cut(s) 41
PfeI GAWTC 6 cut(s) 86, 273, 588, 869, 953, 1061
PfoI TCCNGGA 1 cut(s) 1179
PkrI GCNGC 7 cut(s) 120, 123, 203, 206, 321, 479, 490
PleI GAGTC 2 cut(s) 371, 629
PpsI GAGTC 2 cut(s) 371, 629
Psp124BI GAGCTC 1 cut(s) 131
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 1171
RseI CAYNNNNRTG 3 cut(s) 309, 396, 963
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 4 cut(s) 36, 137, 891, 1260
SatI GCNGC 7 cut(s) 119, 122, 202, 205, 320, 478, 489
Sau3AI GATC 3 cut(s) 298, 808, 1171
SchI GAGTC 2 cut(s) 371, 629
ScrFI CCNGG 1 cut(s) 1181
SduI GDGCHC 2 cut(s) 131, 248
SfaNI GCATC 5 cut(s) 118, 331, 513, 1017, 1128
SfcI CTRYAG 1 cut(s) 489
SmiMI CAYNNNNRTG 3 cut(s) 309, 396, 963
SmlI CTYRAG 1 cut(s) 438
SmoI CTYRAG 1 cut(s) 438
SseBI AGGCCT 1 cut(s) 41
SspI AATATT 1 cut(s) 94
SspMI CTAG 5 cut(s) 159, 266, 693, 723, 855
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 1179
TaaI ACNGT 4 cut(s) 262, 505, 607, 989
TaqI TCGA 8 cut(s) 242, 255, 339, 407, 518, 530, 941, 1298
TaqII GACCGA 2 cut(s) 138, 540
TauI GCSGC 2 cut(s) 204, 207
TfiI GAWTC 6 cut(s) 86, 273, 588, 869, 953, 1061
Tru1I TTAA 4 cut(s) 36, 137, 891, 1260
Tru9I TTAA 4 cut(s) 36, 137, 891, 1260
TscAI CASTG 2 cut(s) 76, 357
TseI GCWGC 5 cut(s) 118, 121, 319, 477, 488
TspGWI ACGGA 2 cut(s) 684, 1052
TspRI CASTG 2 cut(s) 76, 357
XapI RAATTY 5 cut(s) 409, 514, 712, 880, 1077
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XspI CTAG 5 cut(s) 159, 266, 693, 723, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.